TbVps15 is required for vesicular transport and cytokinesis in Trypanosoma brucei
The class III phosphatidylinositol 3-kinase (PI3K) Vps34 is an important regulator of key cellular functions, including cell growth, survival, intracellular trafficking, autophagy and nutrient sensing. In yeast, Vps34 is associated with the putative serine/threonine protein kinase Vps15, however, it...
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2018
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v219_n_p33_Schoijet http://hdl.handle.net/20.500.12110/paper_01666851_v219_n_p33_Schoijet |
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paper:paper_01666851_v219_n_p33_Schoijet2023-06-08T15:16:03Z TbVps15 is required for vesicular transport and cytokinesis in Trypanosoma brucei Cell cycle Endocytosis TbVps15 protein kinase Trypanosoma brucei concanavalin A dextran phosphatidylinositol 3 kinase transferrin phosphatidylinositol 3 kinase protein binding protein serine threonine kinase VPS15 animal cell Article cell growth cell structure cell vacuole controlled study cytokinesis down regulation endocytosis enzyme activity flagellum growth inhibition nonhuman priority journal protein expression protein function RNA interference scanning electron microscopy transmission electron microscopy Trypanosoma brucei atomic force microscopy cytology disease transmission endocytosis enzymology gene knockdown genetics metabolism physiology Trypanosoma brucei brucei Class III Phosphatidylinositol 3-Kinases Cytokinesis Disease Transmission, Infectious Endocytosis Gene Knockdown Techniques Microscopy, Atomic Force Microscopy, Electron, Transmission Phosphatidylinositol 3-Kinase Protein Binding Trypanosoma brucei brucei Vacuolar Sorting Protein VPS15 The class III phosphatidylinositol 3-kinase (PI3K) Vps34 is an important regulator of key cellular functions, including cell growth, survival, intracellular trafficking, autophagy and nutrient sensing. In yeast, Vps34 is associated with the putative serine/threonine protein kinase Vps15, however, its role in signaling has not been deeply evaluated. Here, we have identified the Vps15 orthologue in Trypanosoma brucei, named TbVps15. Knockdown of TbVps15 expression by interference RNA resulted in inhibition of cell growth and blockage of cytokinesis. Scanning electron microcopy revealed a variety of morphological abnormalities, with enlarged parasites and dividing cells that often exhibited a detached flagellum. Transmission electron microscopy analysis of TbVps15 RNAi cells showed an increase in intracellular vacuoles of the endomembrane system and some cells displayed an enlargement of the flagellar pocket, a common feature of cells defective in endocytosis. Moreover, uptake of dextran, transferrin and Concanavalin A was impaired. Finally, TbVps15 downregulation affected the PI3K activity, supporting the hypothesis that TbVps15 and TbVps34 form a complex as occurs in other organisms. In summary, we propose that TbVps15 has a role in the maintenance of cytokinesis, endocytosis and intracellular trafficking in T. brucei. © 2017 Elsevier B.V. 2018 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v219_n_p33_Schoijet http://hdl.handle.net/20.500.12110/paper_01666851_v219_n_p33_Schoijet |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Cell cycle Endocytosis TbVps15 protein kinase Trypanosoma brucei concanavalin A dextran phosphatidylinositol 3 kinase transferrin phosphatidylinositol 3 kinase protein binding protein serine threonine kinase VPS15 animal cell Article cell growth cell structure cell vacuole controlled study cytokinesis down regulation endocytosis enzyme activity flagellum growth inhibition nonhuman priority journal protein expression protein function RNA interference scanning electron microscopy transmission electron microscopy Trypanosoma brucei atomic force microscopy cytology disease transmission endocytosis enzymology gene knockdown genetics metabolism physiology Trypanosoma brucei brucei Class III Phosphatidylinositol 3-Kinases Cytokinesis Disease Transmission, Infectious Endocytosis Gene Knockdown Techniques Microscopy, Atomic Force Microscopy, Electron, Transmission Phosphatidylinositol 3-Kinase Protein Binding Trypanosoma brucei brucei Vacuolar Sorting Protein VPS15 |
| spellingShingle |
Cell cycle Endocytosis TbVps15 protein kinase Trypanosoma brucei concanavalin A dextran phosphatidylinositol 3 kinase transferrin phosphatidylinositol 3 kinase protein binding protein serine threonine kinase VPS15 animal cell Article cell growth cell structure cell vacuole controlled study cytokinesis down regulation endocytosis enzyme activity flagellum growth inhibition nonhuman priority journal protein expression protein function RNA interference scanning electron microscopy transmission electron microscopy Trypanosoma brucei atomic force microscopy cytology disease transmission endocytosis enzymology gene knockdown genetics metabolism physiology Trypanosoma brucei brucei Class III Phosphatidylinositol 3-Kinases Cytokinesis Disease Transmission, Infectious Endocytosis Gene Knockdown Techniques Microscopy, Atomic Force Microscopy, Electron, Transmission Phosphatidylinositol 3-Kinase Protein Binding Trypanosoma brucei brucei Vacuolar Sorting Protein VPS15 TbVps15 is required for vesicular transport and cytokinesis in Trypanosoma brucei |
| topic_facet |
Cell cycle Endocytosis TbVps15 protein kinase Trypanosoma brucei concanavalin A dextran phosphatidylinositol 3 kinase transferrin phosphatidylinositol 3 kinase protein binding protein serine threonine kinase VPS15 animal cell Article cell growth cell structure cell vacuole controlled study cytokinesis down regulation endocytosis enzyme activity flagellum growth inhibition nonhuman priority journal protein expression protein function RNA interference scanning electron microscopy transmission electron microscopy Trypanosoma brucei atomic force microscopy cytology disease transmission endocytosis enzymology gene knockdown genetics metabolism physiology Trypanosoma brucei brucei Class III Phosphatidylinositol 3-Kinases Cytokinesis Disease Transmission, Infectious Endocytosis Gene Knockdown Techniques Microscopy, Atomic Force Microscopy, Electron, Transmission Phosphatidylinositol 3-Kinase Protein Binding Trypanosoma brucei brucei Vacuolar Sorting Protein VPS15 |
| description |
The class III phosphatidylinositol 3-kinase (PI3K) Vps34 is an important regulator of key cellular functions, including cell growth, survival, intracellular trafficking, autophagy and nutrient sensing. In yeast, Vps34 is associated with the putative serine/threonine protein kinase Vps15, however, its role in signaling has not been deeply evaluated. Here, we have identified the Vps15 orthologue in Trypanosoma brucei, named TbVps15. Knockdown of TbVps15 expression by interference RNA resulted in inhibition of cell growth and blockage of cytokinesis. Scanning electron microcopy revealed a variety of morphological abnormalities, with enlarged parasites and dividing cells that often exhibited a detached flagellum. Transmission electron microscopy analysis of TbVps15 RNAi cells showed an increase in intracellular vacuoles of the endomembrane system and some cells displayed an enlargement of the flagellar pocket, a common feature of cells defective in endocytosis. Moreover, uptake of dextran, transferrin and Concanavalin A was impaired. Finally, TbVps15 downregulation affected the PI3K activity, supporting the hypothesis that TbVps15 and TbVps34 form a complex as occurs in other organisms. In summary, we propose that TbVps15 has a role in the maintenance of cytokinesis, endocytosis and intracellular trafficking in T. brucei. © 2017 Elsevier B.V. |
| title |
TbVps15 is required for vesicular transport and cytokinesis in Trypanosoma brucei |
| title_short |
TbVps15 is required for vesicular transport and cytokinesis in Trypanosoma brucei |
| title_full |
TbVps15 is required for vesicular transport and cytokinesis in Trypanosoma brucei |
| title_fullStr |
TbVps15 is required for vesicular transport and cytokinesis in Trypanosoma brucei |
| title_full_unstemmed |
TbVps15 is required for vesicular transport and cytokinesis in Trypanosoma brucei |
| title_sort |
tbvps15 is required for vesicular transport and cytokinesis in trypanosoma brucei |
| publishDate |
2018 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v219_n_p33_Schoijet http://hdl.handle.net/20.500.12110/paper_01666851_v219_n_p33_Schoijet |
| _version_ |
1768542215748976640 |