Host Epac1 is required for cAMP-mediated invasion by Trypanosoma cruzi

Mechanistic details of the modulation by cAMP of Trypanosoma cruzi host cell invasion remain ill-defined. Here we report that activation of host's Epac1 stimulated invasion, whereas specific pharmacological inhibition or maneuvers that alter Epac1 subcellular localization significantly reduced...

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Autores principales:	Musikant, Alejandro Daniel, Buscaglia, Carlos Andrés, Altschuler, Daniel Leonardo, Edreira, Martín M.
Publicado:	2017
Materias:	cAMP Epac Invasion PKA Trypanosoma cruzi cyclic AMP cyclic AMP dependent protein kinase Epac1 protein protozoal protein unclassified drug Epac-1 protein, rat guanine nucleotide exchange factor Article cellular distribution host cell nonhuman priority journal protein localization protein synthesis signal transduction animal cell culture host parasite interaction metabolism pathogenicity physiology protein transport rat Animals Cells, Cultured Cyclic AMP Cyclic AMP-Dependent Protein Kinases Guanine Nucleotide Exchange Factors Host-Parasite Interactions Protein Transport Rats Signal Transduction
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v211_n_p67_Musikant http://hdl.handle.net/20.500.12110/paper_01666851_v211_n_p67_Musikant
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_01666851_v211_n_p67_Musikant
record_format	dspace
spelling	paper:paper_01666851_v211_n_p67_Musikant2023-06-08T15:16:02Z Host Epac1 is required for cAMP-mediated invasion by Trypanosoma cruzi Musikant, Alejandro Daniel Buscaglia, Carlos Andrés Altschuler, Daniel Leonardo Edreira, Martín M. cAMP Epac Invasion PKA Trypanosoma cruzi cyclic AMP cyclic AMP dependent protein kinase Epac1 protein protozoal protein unclassified drug cyclic AMP Epac-1 protein, rat guanine nucleotide exchange factor Article cellular distribution host cell nonhuman priority journal protein localization protein synthesis signal transduction Trypanosoma cruzi animal cell culture host parasite interaction metabolism pathogenicity physiology protein transport rat Trypanosoma cruzi Animals Cells, Cultured Cyclic AMP Cyclic AMP-Dependent Protein Kinases Guanine Nucleotide Exchange Factors Host-Parasite Interactions Protein Transport Rats Signal Transduction Trypanosoma cruzi Mechanistic details of the modulation by cAMP of Trypanosoma cruzi host cell invasion remain ill-defined. Here we report that activation of host's Epac1 stimulated invasion, whereas specific pharmacological inhibition or maneuvers that alter Epac1 subcellular localization significantly reduced invasion. Furthermore, while specific activation of host cell PKA showed no effect, its inhibition resulted in an increased invasion, revealing a crosstalk between the PKA and Epac signaling pathways during the process of invasion. Therefore, our data suggests that subcellular localization of Epac might be playing an important role during invasion and that specific activation of the host cell cAMP/Epac1 pathway is required for cAMP-mediated invasion. © 2016 Elsevier B.V. Fil:Musikant, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Buscaglia, C.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Altschuler, D.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Edreira, M.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2017 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v211_n_p67_Musikant http://hdl.handle.net/20.500.12110/paper_01666851_v211_n_p67_Musikant
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	cAMP Epac Invasion PKA Trypanosoma cruzi cyclic AMP cyclic AMP dependent protein kinase Epac1 protein protozoal protein unclassified drug cyclic AMP Epac-1 protein, rat guanine nucleotide exchange factor Article cellular distribution host cell nonhuman priority journal protein localization protein synthesis signal transduction Trypanosoma cruzi animal cell culture host parasite interaction metabolism pathogenicity physiology protein transport rat Trypanosoma cruzi Animals Cells, Cultured Cyclic AMP Cyclic AMP-Dependent Protein Kinases Guanine Nucleotide Exchange Factors Host-Parasite Interactions Protein Transport Rats Signal Transduction Trypanosoma cruzi
spellingShingle	cAMP Epac Invasion PKA Trypanosoma cruzi cyclic AMP cyclic AMP dependent protein kinase Epac1 protein protozoal protein unclassified drug cyclic AMP Epac-1 protein, rat guanine nucleotide exchange factor Article cellular distribution host cell nonhuman priority journal protein localization protein synthesis signal transduction Trypanosoma cruzi animal cell culture host parasite interaction metabolism pathogenicity physiology protein transport rat Trypanosoma cruzi Animals Cells, Cultured Cyclic AMP Cyclic AMP-Dependent Protein Kinases Guanine Nucleotide Exchange Factors Host-Parasite Interactions Protein Transport Rats Signal Transduction Trypanosoma cruzi Musikant, Alejandro Daniel Buscaglia, Carlos Andrés Altschuler, Daniel Leonardo Edreira, Martín M. Host Epac1 is required for cAMP-mediated invasion by Trypanosoma cruzi
topic_facet	cAMP Epac Invasion PKA Trypanosoma cruzi cyclic AMP cyclic AMP dependent protein kinase Epac1 protein protozoal protein unclassified drug cyclic AMP Epac-1 protein, rat guanine nucleotide exchange factor Article cellular distribution host cell nonhuman priority journal protein localization protein synthesis signal transduction Trypanosoma cruzi animal cell culture host parasite interaction metabolism pathogenicity physiology protein transport rat Trypanosoma cruzi Animals Cells, Cultured Cyclic AMP Cyclic AMP-Dependent Protein Kinases Guanine Nucleotide Exchange Factors Host-Parasite Interactions Protein Transport Rats Signal Transduction Trypanosoma cruzi
description	Mechanistic details of the modulation by cAMP of Trypanosoma cruzi host cell invasion remain ill-defined. Here we report that activation of host's Epac1 stimulated invasion, whereas specific pharmacological inhibition or maneuvers that alter Epac1 subcellular localization significantly reduced invasion. Furthermore, while specific activation of host cell PKA showed no effect, its inhibition resulted in an increased invasion, revealing a crosstalk between the PKA and Epac signaling pathways during the process of invasion. Therefore, our data suggests that subcellular localization of Epac might be playing an important role during invasion and that specific activation of the host cell cAMP/Epac1 pathway is required for cAMP-mediated invasion. © 2016 Elsevier B.V.
author	Musikant, Alejandro Daniel Buscaglia, Carlos Andrés Altschuler, Daniel Leonardo Edreira, Martín M.
author_facet	Musikant, Alejandro Daniel Buscaglia, Carlos Andrés Altschuler, Daniel Leonardo Edreira, Martín M.
author_sort	Musikant, Alejandro Daniel
title	Host Epac1 is required for cAMP-mediated invasion by Trypanosoma cruzi
title_short	Host Epac1 is required for cAMP-mediated invasion by Trypanosoma cruzi
title_full	Host Epac1 is required for cAMP-mediated invasion by Trypanosoma cruzi
title_fullStr	Host Epac1 is required for cAMP-mediated invasion by Trypanosoma cruzi
title_full_unstemmed	Host Epac1 is required for cAMP-mediated invasion by Trypanosoma cruzi
title_sort	host epac1 is required for camp-mediated invasion by trypanosoma cruzi
publishDate	2017
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v211_n_p67_Musikant http://hdl.handle.net/20.500.12110/paper_01666851_v211_n_p67_Musikant
work_keys_str_mv	AT musikantalejandrodaniel hostepac1isrequiredforcampmediatedinvasionbytrypanosomacruzi AT buscagliacarlosandres hostepac1isrequiredforcampmediatedinvasionbytrypanosomacruzi AT altschulerdanielleonardo hostepac1isrequiredforcampmediatedinvasionbytrypanosomacruzi AT edreiramartinm hostepac1isrequiredforcampmediatedinvasionbytrypanosomacruzi
_version_	1768545000016052224

Host Epac1 is required for cAMP-mediated invasion by Trypanosoma cruzi

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