Calmodulin and Ca2+-dependent cyclic AMP phosphodiesterase activity in Trypanosoma cruzi

Calmodulin has been purified from Trypanosoma cruzi epimastigote forms by ion-exchange chromatography, gel filtration and affinity chromatography on 2-chloro-10-(3-aminopropyl)phenotiazine-Sepharose. Upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the factor showed a polypeptide band...

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Autores principales:	Torruella, Mónica, Torres, Héctor Norberto
Publicado:	1985
Materias:	Calcium Calmodulin Cyclic AMP Phosphodiesterase Trypanosoma cruzi adenosine phosphate calcium calmodulin enzyme phosphodiesterase 3',5' cyclic nucleotide phosphodiesterase animal cell metabolism nonhuman priority journal protozoon trypanosoma cruzi animal article brain cortex cattle comparative study enzymology isolation and purification kinetics Neurospora crassa 3',5'-Cyclic-Nucleotide Phosphodiesterase Animal Cattle Cerebral Cortex Comparative Study Kinetics Support, Non-U.S. Gov't
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v17_n2_p143_Tellezinon http://hdl.handle.net/20.500.12110/paper_01666851_v17_n2_p143_Tellezinon
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_01666851_v17_n2_p143_Tellezinon
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spelling	paper:paper_01666851_v17_n2_p143_Tellezinon2023-06-08T15:16:01Z Calmodulin and Ca2+-dependent cyclic AMP phosphodiesterase activity in Trypanosoma cruzi Torruella, Mónica Torres, Héctor Norberto Calcium Calmodulin Cyclic AMP Phosphodiesterase Trypanosoma cruzi adenosine phosphate calcium calmodulin enzyme phosphodiesterase 3',5' cyclic nucleotide phosphodiesterase calcium calmodulin animal cell metabolism nonhuman priority journal protozoon trypanosoma cruzi animal article brain cortex cattle comparative study enzymology isolation and purification kinetics Neurospora crassa Trypanosoma cruzi 3',5'-Cyclic-Nucleotide Phosphodiesterase Animal Calcium Calmodulin Cattle Cerebral Cortex Comparative Study Kinetics Neurospora crassa Support, Non-U.S. Gov't Trypanosoma cruzi Calmodulin has been purified from Trypanosoma cruzi epimastigote forms by ion-exchange chromatography, gel filtration and affinity chromatography on 2-chloro-10-(3-aminopropyl)phenotiazine-Sepharose. Upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the factor showed a polypeptide band with an apparent molecular weight of 16 000. In addition, cyclic AMP phosphodiesterase activity from T. cruzi epimastigote forms was purified by ion-exchange chromatography and affinity chromatography on a brain calmodulin-Sepharose column. The enzyme was activated by homologous calmodulin as well as by bovine brain and Neurospora crassa calmodulins. The activation required micromolar concentrations of Ca2+ and it was blocked by EGTA and by some neuroleptic drugs such as chlorpromazine, fluphenazine and compound 48 80. Activations were observed at micromolar concentrations of cyclic AMP as substrate. In addition, T. cruzi calmodulin was also active in bringing about the stimulation of brain phosphodiesterase. © 1985. Fil:Torruella, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Torres, H.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1985 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v17_n2_p143_Tellezinon http://hdl.handle.net/20.500.12110/paper_01666851_v17_n2_p143_Tellezinon
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Calcium Calmodulin Cyclic AMP Phosphodiesterase Trypanosoma cruzi adenosine phosphate calcium calmodulin enzyme phosphodiesterase 3',5' cyclic nucleotide phosphodiesterase calcium calmodulin animal cell metabolism nonhuman priority journal protozoon trypanosoma cruzi animal article brain cortex cattle comparative study enzymology isolation and purification kinetics Neurospora crassa Trypanosoma cruzi 3',5'-Cyclic-Nucleotide Phosphodiesterase Animal Calcium Calmodulin Cattle Cerebral Cortex Comparative Study Kinetics Neurospora crassa Support, Non-U.S. Gov't Trypanosoma cruzi
spellingShingle	Calcium Calmodulin Cyclic AMP Phosphodiesterase Trypanosoma cruzi adenosine phosphate calcium calmodulin enzyme phosphodiesterase 3',5' cyclic nucleotide phosphodiesterase calcium calmodulin animal cell metabolism nonhuman priority journal protozoon trypanosoma cruzi animal article brain cortex cattle comparative study enzymology isolation and purification kinetics Neurospora crassa Trypanosoma cruzi 3',5'-Cyclic-Nucleotide Phosphodiesterase Animal Calcium Calmodulin Cattle Cerebral Cortex Comparative Study Kinetics Neurospora crassa Support, Non-U.S. Gov't Trypanosoma cruzi Torruella, Mónica Torres, Héctor Norberto Calmodulin and Ca2+-dependent cyclic AMP phosphodiesterase activity in Trypanosoma cruzi
topic_facet	Calcium Calmodulin Cyclic AMP Phosphodiesterase Trypanosoma cruzi adenosine phosphate calcium calmodulin enzyme phosphodiesterase 3',5' cyclic nucleotide phosphodiesterase calcium calmodulin animal cell metabolism nonhuman priority journal protozoon trypanosoma cruzi animal article brain cortex cattle comparative study enzymology isolation and purification kinetics Neurospora crassa Trypanosoma cruzi 3',5'-Cyclic-Nucleotide Phosphodiesterase Animal Calcium Calmodulin Cattle Cerebral Cortex Comparative Study Kinetics Neurospora crassa Support, Non-U.S. Gov't Trypanosoma cruzi
description	Calmodulin has been purified from Trypanosoma cruzi epimastigote forms by ion-exchange chromatography, gel filtration and affinity chromatography on 2-chloro-10-(3-aminopropyl)phenotiazine-Sepharose. Upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the factor showed a polypeptide band with an apparent molecular weight of 16 000. In addition, cyclic AMP phosphodiesterase activity from T. cruzi epimastigote forms was purified by ion-exchange chromatography and affinity chromatography on a brain calmodulin-Sepharose column. The enzyme was activated by homologous calmodulin as well as by bovine brain and Neurospora crassa calmodulins. The activation required micromolar concentrations of Ca2+ and it was blocked by EGTA and by some neuroleptic drugs such as chlorpromazine, fluphenazine and compound 48 80. Activations were observed at micromolar concentrations of cyclic AMP as substrate. In addition, T. cruzi calmodulin was also active in bringing about the stimulation of brain phosphodiesterase. © 1985.
author	Torruella, Mónica Torres, Héctor Norberto
author_facet	Torruella, Mónica Torres, Héctor Norberto
author_sort	Torruella, Mónica
title	Calmodulin and Ca2+-dependent cyclic AMP phosphodiesterase activity in Trypanosoma cruzi
title_short	Calmodulin and Ca2+-dependent cyclic AMP phosphodiesterase activity in Trypanosoma cruzi
title_full	Calmodulin and Ca2+-dependent cyclic AMP phosphodiesterase activity in Trypanosoma cruzi
title_fullStr	Calmodulin and Ca2+-dependent cyclic AMP phosphodiesterase activity in Trypanosoma cruzi
title_full_unstemmed	Calmodulin and Ca2+-dependent cyclic AMP phosphodiesterase activity in Trypanosoma cruzi
title_sort	calmodulin and ca2+-dependent cyclic amp phosphodiesterase activity in trypanosoma cruzi
publishDate	1985
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v17_n2_p143_Tellezinon http://hdl.handle.net/20.500.12110/paper_01666851_v17_n2_p143_Tellezinon
work_keys_str_mv	AT torruellamonica calmodulinandca2dependentcyclicampphosphodiesteraseactivityintrypanosomacruzi AT torreshectornorberto calmodulinandca2dependentcyclicampphosphodiesteraseactivityintrypanosomacruzi
_version_	1768545369433571328

Calmodulin and Ca2+-dependent cyclic AMP phosphodiesterase activity in Trypanosoma cruzi

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