The Trypanosoma cruzi PIN1 gene encodes a parvulin peptidyl-prolyl cis/trans isomerase able to replace the essential ESS1 in Saccharomyces cerevisiae

Parvulins are a conserved group of peptidyl-prolyl cis/trans isomerases (PPIases) that catalyze the cis/trans isomerization of proline-preceding peptide bonds. Parvulin-class PPIases are structurally unrelated to cyclophilins and FK506-binding proteins that are defined as receptors for immunosuppres...

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Autor principal:	Erben, Esteban Daniel
Publicado:	2007
Materias:	Cell cycle Parvulin Pin1 Prolyl cis/trans isomerization Trypanosoma cruzi Trypanosomatids amino acid derivative peptidylprolyl isomerase Pin1 protein parvulin Saccharomyces cerevisiae protein unclassified drug amino terminal sequence animal cell article catalysis cell structure gene mutation isomerization nonhuman phenotype priority journal protein analysis temperature Western blotting Amino Acid Sequence Animals Cell Cycle Gene Expression Regulation Genetic Complementation Test Humans Molecular Sequence Data Peptidylprolyl Isomerase Protozoan Proteins Recombinant Proteins Sequence Alignment Sequence Analysis, DNA Saccharomyces cerevisiae Trypanosomatidae
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v153_n2_p186_Erben http://hdl.handle.net/20.500.12110/paper_01666851_v153_n2_p186_Erben
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_01666851_v153_n2_p186_Erben
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spelling	paper:paper_01666851_v153_n2_p186_Erben2023-06-08T15:15:58Z The Trypanosoma cruzi PIN1 gene encodes a parvulin peptidyl-prolyl cis/trans isomerase able to replace the essential ESS1 in Saccharomyces cerevisiae Erben, Esteban Daniel Cell cycle Parvulin Pin1 Prolyl cis/trans isomerization Trypanosoma cruzi Trypanosomatids amino acid derivative peptidylprolyl isomerase Pin1 protein parvulin Saccharomyces cerevisiae protein unclassified drug amino terminal sequence animal cell article catalysis cell structure gene mutation isomerization nonhuman phenotype priority journal protein analysis temperature Trypanosoma cruzi Western blotting Amino Acid Sequence Animals Cell Cycle Gene Expression Regulation Genetic Complementation Test Humans Molecular Sequence Data Peptidylprolyl Isomerase Protozoan Proteins Recombinant Proteins Sequence Alignment Sequence Analysis, DNA Trypanosoma cruzi Saccharomyces cerevisiae Trypanosoma cruzi Trypanosomatidae Parvulins are a conserved group of peptidyl-prolyl cis/trans isomerases (PPIases) that catalyze the cis/trans isomerization of proline-preceding peptide bonds. Parvulin-class PPIases are structurally unrelated to cyclophilins and FK506-binding proteins that are defined as receptors for immunosuppressive drugs. In Trypanosoma cruzi we identified parvulin TcPIN1 as a homolog of the human hPin1 PPIase. The 117 amino acids of the TcPIN1 display 40% identity with the catalytic core of hPin1 and exhibit prolyl cis/trans isomerase activity. TcPIN1 lacks the WW domain at the N-terminus, and is able to rescue the temperature-sensitive phenotype on a mutation in the Saccharomyces cerevisiae hPin1 homolog, ESS1/PTF1. Western blot analysis revealed that the enzyme was present both in dividing and non-dividing forms of T. cruzi. In epimastigote cells neither cell growth kinetics nor cell morphology was affected by the overexpression of the small parvulin TcPIN1. These results suggest the occurrence of a supplementary conserved level of post-translational control in trypanosomatids. © 2007 Elsevier B.V. All rights reserved. Fil:Erben, E.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2007 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v153_n2_p186_Erben http://hdl.handle.net/20.500.12110/paper_01666851_v153_n2_p186_Erben
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Cell cycle Parvulin Pin1 Prolyl cis/trans isomerization Trypanosoma cruzi Trypanosomatids amino acid derivative peptidylprolyl isomerase Pin1 protein parvulin Saccharomyces cerevisiae protein unclassified drug amino terminal sequence animal cell article catalysis cell structure gene mutation isomerization nonhuman phenotype priority journal protein analysis temperature Trypanosoma cruzi Western blotting Amino Acid Sequence Animals Cell Cycle Gene Expression Regulation Genetic Complementation Test Humans Molecular Sequence Data Peptidylprolyl Isomerase Protozoan Proteins Recombinant Proteins Sequence Alignment Sequence Analysis, DNA Trypanosoma cruzi Saccharomyces cerevisiae Trypanosoma cruzi Trypanosomatidae
spellingShingle	Cell cycle Parvulin Pin1 Prolyl cis/trans isomerization Trypanosoma cruzi Trypanosomatids amino acid derivative peptidylprolyl isomerase Pin1 protein parvulin Saccharomyces cerevisiae protein unclassified drug amino terminal sequence animal cell article catalysis cell structure gene mutation isomerization nonhuman phenotype priority journal protein analysis temperature Trypanosoma cruzi Western blotting Amino Acid Sequence Animals Cell Cycle Gene Expression Regulation Genetic Complementation Test Humans Molecular Sequence Data Peptidylprolyl Isomerase Protozoan Proteins Recombinant Proteins Sequence Alignment Sequence Analysis, DNA Trypanosoma cruzi Saccharomyces cerevisiae Trypanosoma cruzi Trypanosomatidae Erben, Esteban Daniel The Trypanosoma cruzi PIN1 gene encodes a parvulin peptidyl-prolyl cis/trans isomerase able to replace the essential ESS1 in Saccharomyces cerevisiae
topic_facet	Cell cycle Parvulin Pin1 Prolyl cis/trans isomerization Trypanosoma cruzi Trypanosomatids amino acid derivative peptidylprolyl isomerase Pin1 protein parvulin Saccharomyces cerevisiae protein unclassified drug amino terminal sequence animal cell article catalysis cell structure gene mutation isomerization nonhuman phenotype priority journal protein analysis temperature Trypanosoma cruzi Western blotting Amino Acid Sequence Animals Cell Cycle Gene Expression Regulation Genetic Complementation Test Humans Molecular Sequence Data Peptidylprolyl Isomerase Protozoan Proteins Recombinant Proteins Sequence Alignment Sequence Analysis, DNA Trypanosoma cruzi Saccharomyces cerevisiae Trypanosoma cruzi Trypanosomatidae
description	Parvulins are a conserved group of peptidyl-prolyl cis/trans isomerases (PPIases) that catalyze the cis/trans isomerization of proline-preceding peptide bonds. Parvulin-class PPIases are structurally unrelated to cyclophilins and FK506-binding proteins that are defined as receptors for immunosuppressive drugs. In Trypanosoma cruzi we identified parvulin TcPIN1 as a homolog of the human hPin1 PPIase. The 117 amino acids of the TcPIN1 display 40% identity with the catalytic core of hPin1 and exhibit prolyl cis/trans isomerase activity. TcPIN1 lacks the WW domain at the N-terminus, and is able to rescue the temperature-sensitive phenotype on a mutation in the Saccharomyces cerevisiae hPin1 homolog, ESS1/PTF1. Western blot analysis revealed that the enzyme was present both in dividing and non-dividing forms of T. cruzi. In epimastigote cells neither cell growth kinetics nor cell morphology was affected by the overexpression of the small parvulin TcPIN1. These results suggest the occurrence of a supplementary conserved level of post-translational control in trypanosomatids. © 2007 Elsevier B.V. All rights reserved.
author	Erben, Esteban Daniel
author_facet	Erben, Esteban Daniel
author_sort	Erben, Esteban Daniel
title	The Trypanosoma cruzi PIN1 gene encodes a parvulin peptidyl-prolyl cis/trans isomerase able to replace the essential ESS1 in Saccharomyces cerevisiae
title_short	The Trypanosoma cruzi PIN1 gene encodes a parvulin peptidyl-prolyl cis/trans isomerase able to replace the essential ESS1 in Saccharomyces cerevisiae
title_full	The Trypanosoma cruzi PIN1 gene encodes a parvulin peptidyl-prolyl cis/trans isomerase able to replace the essential ESS1 in Saccharomyces cerevisiae
title_fullStr	The Trypanosoma cruzi PIN1 gene encodes a parvulin peptidyl-prolyl cis/trans isomerase able to replace the essential ESS1 in Saccharomyces cerevisiae
title_full_unstemmed	The Trypanosoma cruzi PIN1 gene encodes a parvulin peptidyl-prolyl cis/trans isomerase able to replace the essential ESS1 in Saccharomyces cerevisiae
title_sort	trypanosoma cruzi pin1 gene encodes a parvulin peptidyl-prolyl cis/trans isomerase able to replace the essential ess1 in saccharomyces cerevisiae
publishDate	2007
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v153_n2_p186_Erben http://hdl.handle.net/20.500.12110/paper_01666851_v153_n2_p186_Erben
work_keys_str_mv	AT erbenestebandaniel thetrypanosomacruzipin1geneencodesaparvulinpeptidylprolylcistransisomeraseabletoreplacetheessentialess1insaccharomycescerevisiae AT erbenestebandaniel trypanosomacruzipin1geneencodesaparvulinpeptidylprolylcistransisomeraseabletoreplacetheessentialess1insaccharomycescerevisiae
_version_	1768544128315949056

The Trypanosoma cruzi PIN1 gene encodes a parvulin peptidyl-prolyl cis/trans isomerase able to replace the essential ESS1 in Saccharomyces cerevisiae

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