Trypanosoma cruzi Tcp12CKS1 interacts with parasite CRKs and rescues the p13SUC1 fission yeast mutant

The complex mechanism of cell division in trypanosomatids is not completely fully understood. CRKs (cdc2-related kinases), Cyclins and CKSs (cdc2-kinase subunit) are involved in the progression through the cell cycle. The CKS proteins were first described as components of the cell cycle machinery in...

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Autores principales:	Muñoz, Manuel Javier, Santori, María Isabel, Rojas, Federico, Gómez, Eliana Beatriz
Publicado:	2006
Materias:	Cell cycle CKS CRKs Cyclins Trypanosoma cruzi antiserum cyclin dependent kinase cyclin dependent kinase 1 cyclin dependent kinase inhibitor cycline protein kinase protozoal protein Schizosaccharomyces pombe protein cell cycle protein CRK protein, protozoan Suc1 protein, S pombe article cell cycle enzyme activity enzyme inhibition epimastigote immunoprecipitation nonhuman nucleotide sequence priority journal protein analysis protein interaction Schizosaccharomyces pombe trypomastigote Western blotting yeast amino acid sequence animal chemistry DNA sequence enzymology gene expression regulation genetics growth, development and aging metabolism molecular genetics mutation Schizosaccharomyces Trypanosomatidae Amino Acid Sequence Animals CDC2 Protein Kinase Cell Cycle Proteins Gene Expression Regulation Molecular Sequence Data Mutation Protein Kinases Protozoan Proteins Schizosaccharomyces pombe Proteins Sequence Analysis, DNA
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v147_n2_p154_Munoz http://hdl.handle.net/20.500.12110/paper_01666851_v147_n2_p154_Munoz
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	paper:paper_01666851_v147_n2_p154_Munoz2023-06-08T15:15:58Z Trypanosoma cruzi Tcp12CKS1 interacts with parasite CRKs and rescues the p13SUC1 fission yeast mutant Muñoz, Manuel Javier Santori, María Isabel Rojas, Federico Gómez, Eliana Beatriz Cell cycle CKS CRKs Cyclins Trypanosoma cruzi antiserum cyclin dependent kinase cyclin dependent kinase 1 cyclin dependent kinase inhibitor cycline protein kinase protozoal protein Schizosaccharomyces pombe protein cell cycle protein CRK protein, protozoan protein kinase Suc1 protein, S pombe article cell cycle enzyme activity enzyme inhibition epimastigote immunoprecipitation nonhuman nucleotide sequence priority journal protein analysis protein interaction Schizosaccharomyces pombe Trypanosoma cruzi trypomastigote Western blotting yeast amino acid sequence animal chemistry DNA sequence enzymology gene expression regulation genetics growth, development and aging metabolism molecular genetics mutation Schizosaccharomyces Schizosaccharomyces pombe Trypanosoma cruzi Trypanosomatidae Amino Acid Sequence Animals CDC2 Protein Kinase Cell Cycle Proteins Gene Expression Regulation Molecular Sequence Data Mutation Protein Kinases Protozoan Proteins Schizosaccharomyces Schizosaccharomyces pombe Proteins Sequence Analysis, DNA Trypanosoma cruzi The complex mechanism of cell division in trypanosomatids is not completely fully understood. CRKs (cdc2-related kinases), Cyclins and CKSs (cdc2-kinase subunit) are involved in the progression through the cell cycle. The CKS proteins were first described as components of the cell cycle machinery in yeast and their action has been implicated in the regulation of CDK function. In the present work we identified Tcp12CKS1 a member of the CKS family in the parasite Trypanosoma cruzi. TcCKS1 is expressed in the three forms of T. cruzi. By using anti-Tcp12CKS1 antiserum, protein kinase (PK) activities were immunoprecipitated. The PK activity level varies depending on the stage analyzed, being lower in trypomastigotes and thus suggesting that different stages have different CKS-CRK complexes. Moreover, these PK activities were inhibited by using Flavopiridol, a known CDKs inhibitor. Western blot analyses demonstrated that in the epimastigote stage, p12CKS1 stably interacts with TcCRK1 and TcCRK3. In addition, Tcp12CKS1 was able to rescue the p13SUC1 null mutant of S. pombe. The functional complementation between the CKS proteins of two evolutionary distant organisms supports the role of Tcp12CKS1 as a key regulator in T. cruzi cell cycle. © 2006 Elsevier B.V. All rights reserved. Fil:Muñoz, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Santori, M.I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rojas, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Gómez, E.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2006 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v147_n2_p154_Munoz http://hdl.handle.net/20.500.12110/paper_01666851_v147_n2_p154_Munoz
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Cell cycle CKS CRKs Cyclins Trypanosoma cruzi antiserum cyclin dependent kinase cyclin dependent kinase 1 cyclin dependent kinase inhibitor cycline protein kinase protozoal protein Schizosaccharomyces pombe protein cell cycle protein CRK protein, protozoan protein kinase Suc1 protein, S pombe article cell cycle enzyme activity enzyme inhibition epimastigote immunoprecipitation nonhuman nucleotide sequence priority journal protein analysis protein interaction Schizosaccharomyces pombe Trypanosoma cruzi trypomastigote Western blotting yeast amino acid sequence animal chemistry DNA sequence enzymology gene expression regulation genetics growth, development and aging metabolism molecular genetics mutation Schizosaccharomyces Schizosaccharomyces pombe Trypanosoma cruzi Trypanosomatidae Amino Acid Sequence Animals CDC2 Protein Kinase Cell Cycle Proteins Gene Expression Regulation Molecular Sequence Data Mutation Protein Kinases Protozoan Proteins Schizosaccharomyces Schizosaccharomyces pombe Proteins Sequence Analysis, DNA Trypanosoma cruzi
spellingShingle	Cell cycle CKS CRKs Cyclins Trypanosoma cruzi antiserum cyclin dependent kinase cyclin dependent kinase 1 cyclin dependent kinase inhibitor cycline protein kinase protozoal protein Schizosaccharomyces pombe protein cell cycle protein CRK protein, protozoan protein kinase Suc1 protein, S pombe article cell cycle enzyme activity enzyme inhibition epimastigote immunoprecipitation nonhuman nucleotide sequence priority journal protein analysis protein interaction Schizosaccharomyces pombe Trypanosoma cruzi trypomastigote Western blotting yeast amino acid sequence animal chemistry DNA sequence enzymology gene expression regulation genetics growth, development and aging metabolism molecular genetics mutation Schizosaccharomyces Schizosaccharomyces pombe Trypanosoma cruzi Trypanosomatidae Amino Acid Sequence Animals CDC2 Protein Kinase Cell Cycle Proteins Gene Expression Regulation Molecular Sequence Data Mutation Protein Kinases Protozoan Proteins Schizosaccharomyces Schizosaccharomyces pombe Proteins Sequence Analysis, DNA Trypanosoma cruzi Muñoz, Manuel Javier Santori, María Isabel Rojas, Federico Gómez, Eliana Beatriz Trypanosoma cruzi Tcp12CKS1 interacts with parasite CRKs and rescues the p13SUC1 fission yeast mutant
topic_facet	Cell cycle CKS CRKs Cyclins Trypanosoma cruzi antiserum cyclin dependent kinase cyclin dependent kinase 1 cyclin dependent kinase inhibitor cycline protein kinase protozoal protein Schizosaccharomyces pombe protein cell cycle protein CRK protein, protozoan protein kinase Suc1 protein, S pombe article cell cycle enzyme activity enzyme inhibition epimastigote immunoprecipitation nonhuman nucleotide sequence priority journal protein analysis protein interaction Schizosaccharomyces pombe Trypanosoma cruzi trypomastigote Western blotting yeast amino acid sequence animal chemistry DNA sequence enzymology gene expression regulation genetics growth, development and aging metabolism molecular genetics mutation Schizosaccharomyces Schizosaccharomyces pombe Trypanosoma cruzi Trypanosomatidae Amino Acid Sequence Animals CDC2 Protein Kinase Cell Cycle Proteins Gene Expression Regulation Molecular Sequence Data Mutation Protein Kinases Protozoan Proteins Schizosaccharomyces Schizosaccharomyces pombe Proteins Sequence Analysis, DNA Trypanosoma cruzi
description	The complex mechanism of cell division in trypanosomatids is not completely fully understood. CRKs (cdc2-related kinases), Cyclins and CKSs (cdc2-kinase subunit) are involved in the progression through the cell cycle. The CKS proteins were first described as components of the cell cycle machinery in yeast and their action has been implicated in the regulation of CDK function. In the present work we identified Tcp12CKS1 a member of the CKS family in the parasite Trypanosoma cruzi. TcCKS1 is expressed in the three forms of T. cruzi. By using anti-Tcp12CKS1 antiserum, protein kinase (PK) activities were immunoprecipitated. The PK activity level varies depending on the stage analyzed, being lower in trypomastigotes and thus suggesting that different stages have different CKS-CRK complexes. Moreover, these PK activities were inhibited by using Flavopiridol, a known CDKs inhibitor. Western blot analyses demonstrated that in the epimastigote stage, p12CKS1 stably interacts with TcCRK1 and TcCRK3. In addition, Tcp12CKS1 was able to rescue the p13SUC1 null mutant of S. pombe. The functional complementation between the CKS proteins of two evolutionary distant organisms supports the role of Tcp12CKS1 as a key regulator in T. cruzi cell cycle. © 2006 Elsevier B.V. All rights reserved.
author	Muñoz, Manuel Javier Santori, María Isabel Rojas, Federico Gómez, Eliana Beatriz
author_facet	Muñoz, Manuel Javier Santori, María Isabel Rojas, Federico Gómez, Eliana Beatriz
author_sort	Muñoz, Manuel Javier
title	Trypanosoma cruzi Tcp12CKS1 interacts with parasite CRKs and rescues the p13SUC1 fission yeast mutant
title_short	Trypanosoma cruzi Tcp12CKS1 interacts with parasite CRKs and rescues the p13SUC1 fission yeast mutant
title_full	Trypanosoma cruzi Tcp12CKS1 interacts with parasite CRKs and rescues the p13SUC1 fission yeast mutant
title_fullStr	Trypanosoma cruzi Tcp12CKS1 interacts with parasite CRKs and rescues the p13SUC1 fission yeast mutant
title_full_unstemmed	Trypanosoma cruzi Tcp12CKS1 interacts with parasite CRKs and rescues the p13SUC1 fission yeast mutant
title_sort	trypanosoma cruzi tcp12cks1 interacts with parasite crks and rescues the p13suc1 fission yeast mutant
publishDate	2006
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v147_n2_p154_Munoz http://hdl.handle.net/20.500.12110/paper_01666851_v147_n2_p154_Munoz
work_keys_str_mv	AT munozmanueljavier trypanosomacruzitcp12cks1interactswithparasitecrksandrescuesthep13suc1fissionyeastmutant AT santorimariaisabel trypanosomacruzitcp12cks1interactswithparasitecrksandrescuesthep13suc1fissionyeastmutant AT rojasfederico trypanosomacruzitcp12cks1interactswithparasitecrksandrescuesthep13suc1fissionyeastmutant AT gomezelianabeatriz trypanosomacruzitcp12cks1interactswithparasitecrksandrescuesthep13suc1fissionyeastmutant
_version_	1768541983139168256

Trypanosoma cruzi Tcp12CKS1 interacts with parasite CRKs and rescues the p13SUC1 fission yeast mutant

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