Inositolphosphoceramide is not a substrate for the first steps in the biosynthesis of glycoinositolphospholipids in Trypanosoma cruzi

The major free glycoinositolphospholipids and protein-linked glycoinositolphospholipids in Trypanosoma cruzi contain ceramide as the lipid moiety. Ceramide was not found in mammalian glycosylphosphatidylinositol (GPI)-anchors. An alkylglycerol, either as a lyso species or acylated has been also foun...

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Publicado:	2004
Materias:	Ceramide Glycoinositolphospholipids GPI anchors Inositolphosphoceramide T. cruzi ceramide derivative inositol derivative inositol phosphoceramide unclassified drug acetylation article biosynthesis chemical structure controlled study enzyme degradation epimastigote nonhuman priority journal saponification Trypanosoma cruzi Mammalia Trypanosoma
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v133_n1_p71_Bertello http://hdl.handle.net/20.500.12110/paper_01666851_v133_n1_p71_Bertello
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_01666851_v133_n1_p71_Bertello
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spelling	paper:paper_01666851_v133_n1_p71_Bertello2023-06-08T15:15:55Z Inositolphosphoceramide is not a substrate for the first steps in the biosynthesis of glycoinositolphospholipids in Trypanosoma cruzi Ceramide Glycoinositolphospholipids GPI anchors Inositolphosphoceramide T. cruzi ceramide derivative inositol derivative inositol phosphoceramide unclassified drug acetylation article biosynthesis chemical structure controlled study enzyme degradation epimastigote nonhuman priority journal saponification Trypanosoma cruzi Mammalia Trypanosoma Trypanosoma cruzi The major free glycoinositolphospholipids and protein-linked glycoinositolphospholipids in Trypanosoma cruzi contain ceramide as the lipid moiety. Ceramide was not found in mammalian glycosylphosphatidylinositol (GPI)-anchors. An alkylglycerol, either as a lyso species or acylated has been also found in T. cruzi anchors. However, unlike African trypanosomes, no diacylglycerol was detected in the GPI-anchors. Using a membrane preparation from epimastigotes upon labelling with UDP[3H]GlcNAc we identified [3H]GlcNAcPI as the first step of GPI biosynthesis. Both, alkylacylglycerol (major) and diacylglycerol are constituents of the lipid. Although inositolphosphoceramide is the main inositolphospholipid in epimastigotes, it does not incorporate GlcNAc. The de-N-acetylation step afforded [3H]GlcN(alkylacylglycerol)PI and we also detected the [3H]GlcN(lysoacyl)PI. A new metabolite, phosphoGlcN(lysoacyl)PI, which was formed on long incorporation times, was characterized by chemical and enzymatic degradations. Several [3H]-Man labelled GPI precursors were obtained by in vitro GDP[3H]-Man labelling in the presence of UDPGlcNAc. All of them were sensitive to PI-PLC and to saponification conditions, thus, supporting a glycerolipid structure. © 2003 Elsevier B.V. All rights reserved. 2004 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v133_n1_p71_Bertello http://hdl.handle.net/20.500.12110/paper_01666851_v133_n1_p71_Bertello
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Ceramide Glycoinositolphospholipids GPI anchors Inositolphosphoceramide T. cruzi ceramide derivative inositol derivative inositol phosphoceramide unclassified drug acetylation article biosynthesis chemical structure controlled study enzyme degradation epimastigote nonhuman priority journal saponification Trypanosoma cruzi Mammalia Trypanosoma Trypanosoma cruzi
spellingShingle	Ceramide Glycoinositolphospholipids GPI anchors Inositolphosphoceramide T. cruzi ceramide derivative inositol derivative inositol phosphoceramide unclassified drug acetylation article biosynthesis chemical structure controlled study enzyme degradation epimastigote nonhuman priority journal saponification Trypanosoma cruzi Mammalia Trypanosoma Trypanosoma cruzi Inositolphosphoceramide is not a substrate for the first steps in the biosynthesis of glycoinositolphospholipids in Trypanosoma cruzi
topic_facet	Ceramide Glycoinositolphospholipids GPI anchors Inositolphosphoceramide T. cruzi ceramide derivative inositol derivative inositol phosphoceramide unclassified drug acetylation article biosynthesis chemical structure controlled study enzyme degradation epimastigote nonhuman priority journal saponification Trypanosoma cruzi Mammalia Trypanosoma Trypanosoma cruzi
description	The major free glycoinositolphospholipids and protein-linked glycoinositolphospholipids in Trypanosoma cruzi contain ceramide as the lipid moiety. Ceramide was not found in mammalian glycosylphosphatidylinositol (GPI)-anchors. An alkylglycerol, either as a lyso species or acylated has been also found in T. cruzi anchors. However, unlike African trypanosomes, no diacylglycerol was detected in the GPI-anchors. Using a membrane preparation from epimastigotes upon labelling with UDP[3H]GlcNAc we identified [3H]GlcNAcPI as the first step of GPI biosynthesis. Both, alkylacylglycerol (major) and diacylglycerol are constituents of the lipid. Although inositolphosphoceramide is the main inositolphospholipid in epimastigotes, it does not incorporate GlcNAc. The de-N-acetylation step afforded [3H]GlcN(alkylacylglycerol)PI and we also detected the [3H]GlcN(lysoacyl)PI. A new metabolite, phosphoGlcN(lysoacyl)PI, which was formed on long incorporation times, was characterized by chemical and enzymatic degradations. Several [3H]-Man labelled GPI precursors were obtained by in vitro GDP[3H]-Man labelling in the presence of UDPGlcNAc. All of them were sensitive to PI-PLC and to saponification conditions, thus, supporting a glycerolipid structure. © 2003 Elsevier B.V. All rights reserved.
title	Inositolphosphoceramide is not a substrate for the first steps in the biosynthesis of glycoinositolphospholipids in Trypanosoma cruzi
title_short	Inositolphosphoceramide is not a substrate for the first steps in the biosynthesis of glycoinositolphospholipids in Trypanosoma cruzi
title_full	Inositolphosphoceramide is not a substrate for the first steps in the biosynthesis of glycoinositolphospholipids in Trypanosoma cruzi
title_fullStr	Inositolphosphoceramide is not a substrate for the first steps in the biosynthesis of glycoinositolphospholipids in Trypanosoma cruzi
title_full_unstemmed	Inositolphosphoceramide is not a substrate for the first steps in the biosynthesis of glycoinositolphospholipids in Trypanosoma cruzi
title_sort	inositolphosphoceramide is not a substrate for the first steps in the biosynthesis of glycoinositolphospholipids in trypanosoma cruzi
publishDate	2004
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v133_n1_p71_Bertello http://hdl.handle.net/20.500.12110/paper_01666851_v133_n1_p71_Bertello
_version_	1768544906220929024

Inositolphosphoceramide is not a substrate for the first steps in the biosynthesis of glycoinositolphospholipids in Trypanosoma cruzi

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