Trypanosoma cruzi poly-zinc finger protein: A novel DNA/RNA-binding CCHC-zinc finger protein
A poly-zinc finger protein, designated PZFP1 was identified in Trypanosoma cruzi for the first time. The protein has 191 amino acids, contains seven motifs Cys(X)2Cys(X)4His(X)4Cys. A recombinant PZFP1 was generated in E. coli and the expected 21kDa polypeptide co-purified with two other inducible p...
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2003
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v131_n1_p35_Espinosa http://hdl.handle.net/20.500.12110/paper_01666851_v131_n1_p35_Espinosa |
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paper:paper_01666851_v131_n1_p35_Espinosa2023-06-08T15:15:55Z Trypanosoma cruzi poly-zinc finger protein: A novel DNA/RNA-binding CCHC-zinc finger protein Espinosa, Joaquín Maximiliano Portal, Daniel Lobo, Guillermo Sebastián Pereira, Claudio Alejandro Alonso, Guillermo Daniel Gómez, Eliana Beatriz Flawiá, Mirtha María Torres, Héctor Norberto CCHC-type zinc finger Poly-zinc finger protein Serine-arginine-rich protein TcPZFP1 TcSR Trypanosoma cruzi arginine cell extract double stranded DNA oligonucleotide protein protein antibody protozoal protein recombinant protein serine zinc finger protein amino acid sequence article cell nucleus cellular distribution cytoplasm Escherichia coli gel mobility shift assay immunofluorescence molecular recognition molecular weight nonhuman nucleotide sequence priority journal protein analysis protein DNA interaction protein induction protein localization protein motif protein protein interaction protein purification protein RNA binding Trypanosoma cruzi two hybrid system Western blotting yeast Trypanosoma Trypanosoma cruzi A poly-zinc finger protein, designated PZFP1 was identified in Trypanosoma cruzi for the first time. The protein has 191 amino acids, contains seven motifs Cys(X)2Cys(X)4His(X)4Cys. A recombinant PZFP1 was generated in E. coli and the expected 21kDa polypeptide co-purified with two other inducible products of about 42 and 63kDa. Western blot analysis of cell extracts using an anti-PZFP1 antibody recognized a major band of 41kDa. Electrophoretic mobility shift analysis demonstrated that both, recombinant and native PZFP1, specifically interact with single-stranded DNA or RNA oligonucleotides carrying recognition sequences of other CCHC proteins. The protein was localized mainly in the cytoplasm and nucleus as observed by indirect immunofluorescence analysis. PZFP1 interacted specifically with a T. cruzi serine-arginine-rich protein (TcSR) in a yeast two-hybrid assay, suggesting a role in pre-mRNA processing. © 2003 Elsevier B.V. All rights reserved. Fil:Espinosa, J.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Portal, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lobo, G.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pereira, C.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Alonso, G.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Gómez, E.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Flawiá, M.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Torres, H.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2003 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v131_n1_p35_Espinosa http://hdl.handle.net/20.500.12110/paper_01666851_v131_n1_p35_Espinosa |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
CCHC-type zinc finger Poly-zinc finger protein Serine-arginine-rich protein TcPZFP1 TcSR Trypanosoma cruzi arginine cell extract double stranded DNA oligonucleotide protein protein antibody protozoal protein recombinant protein serine zinc finger protein amino acid sequence article cell nucleus cellular distribution cytoplasm Escherichia coli gel mobility shift assay immunofluorescence molecular recognition molecular weight nonhuman nucleotide sequence priority journal protein analysis protein DNA interaction protein induction protein localization protein motif protein protein interaction protein purification protein RNA binding Trypanosoma cruzi two hybrid system Western blotting yeast Trypanosoma Trypanosoma cruzi |
| spellingShingle |
CCHC-type zinc finger Poly-zinc finger protein Serine-arginine-rich protein TcPZFP1 TcSR Trypanosoma cruzi arginine cell extract double stranded DNA oligonucleotide protein protein antibody protozoal protein recombinant protein serine zinc finger protein amino acid sequence article cell nucleus cellular distribution cytoplasm Escherichia coli gel mobility shift assay immunofluorescence molecular recognition molecular weight nonhuman nucleotide sequence priority journal protein analysis protein DNA interaction protein induction protein localization protein motif protein protein interaction protein purification protein RNA binding Trypanosoma cruzi two hybrid system Western blotting yeast Trypanosoma Trypanosoma cruzi Espinosa, Joaquín Maximiliano Portal, Daniel Lobo, Guillermo Sebastián Pereira, Claudio Alejandro Alonso, Guillermo Daniel Gómez, Eliana Beatriz Flawiá, Mirtha María Torres, Héctor Norberto Trypanosoma cruzi poly-zinc finger protein: A novel DNA/RNA-binding CCHC-zinc finger protein |
| topic_facet |
CCHC-type zinc finger Poly-zinc finger protein Serine-arginine-rich protein TcPZFP1 TcSR Trypanosoma cruzi arginine cell extract double stranded DNA oligonucleotide protein protein antibody protozoal protein recombinant protein serine zinc finger protein amino acid sequence article cell nucleus cellular distribution cytoplasm Escherichia coli gel mobility shift assay immunofluorescence molecular recognition molecular weight nonhuman nucleotide sequence priority journal protein analysis protein DNA interaction protein induction protein localization protein motif protein protein interaction protein purification protein RNA binding Trypanosoma cruzi two hybrid system Western blotting yeast Trypanosoma Trypanosoma cruzi |
| description |
A poly-zinc finger protein, designated PZFP1 was identified in Trypanosoma cruzi for the first time. The protein has 191 amino acids, contains seven motifs Cys(X)2Cys(X)4His(X)4Cys. A recombinant PZFP1 was generated in E. coli and the expected 21kDa polypeptide co-purified with two other inducible products of about 42 and 63kDa. Western blot analysis of cell extracts using an anti-PZFP1 antibody recognized a major band of 41kDa. Electrophoretic mobility shift analysis demonstrated that both, recombinant and native PZFP1, specifically interact with single-stranded DNA or RNA oligonucleotides carrying recognition sequences of other CCHC proteins. The protein was localized mainly in the cytoplasm and nucleus as observed by indirect immunofluorescence analysis. PZFP1 interacted specifically with a T. cruzi serine-arginine-rich protein (TcSR) in a yeast two-hybrid assay, suggesting a role in pre-mRNA processing. © 2003 Elsevier B.V. All rights reserved. |
| author |
Espinosa, Joaquín Maximiliano Portal, Daniel Lobo, Guillermo Sebastián Pereira, Claudio Alejandro Alonso, Guillermo Daniel Gómez, Eliana Beatriz Flawiá, Mirtha María Torres, Héctor Norberto |
| author_facet |
Espinosa, Joaquín Maximiliano Portal, Daniel Lobo, Guillermo Sebastián Pereira, Claudio Alejandro Alonso, Guillermo Daniel Gómez, Eliana Beatriz Flawiá, Mirtha María Torres, Héctor Norberto |
| author_sort |
Espinosa, Joaquín Maximiliano |
| title |
Trypanosoma cruzi poly-zinc finger protein: A novel DNA/RNA-binding CCHC-zinc finger protein |
| title_short |
Trypanosoma cruzi poly-zinc finger protein: A novel DNA/RNA-binding CCHC-zinc finger protein |
| title_full |
Trypanosoma cruzi poly-zinc finger protein: A novel DNA/RNA-binding CCHC-zinc finger protein |
| title_fullStr |
Trypanosoma cruzi poly-zinc finger protein: A novel DNA/RNA-binding CCHC-zinc finger protein |
| title_full_unstemmed |
Trypanosoma cruzi poly-zinc finger protein: A novel DNA/RNA-binding CCHC-zinc finger protein |
| title_sort |
trypanosoma cruzi poly-zinc finger protein: a novel dna/rna-binding cchc-zinc finger protein |
| publishDate |
2003 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v131_n1_p35_Espinosa http://hdl.handle.net/20.500.12110/paper_01666851_v131_n1_p35_Espinosa |
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