An early ancestor in the evolution of splicing: A Trypanosoma cruzi serine-arginine-rich protein (TcSR) is functional in cis-splicing

A novel serine-arginine-rich protein designated TcSR was identified in Trypanosoma cruzi. The deduced amino acid sequence reveals that TcSR is a member of the SR protein family of splicing factors that contains two RNA-binding domains at the N-terminal side and several serine-arginine repeats at the...

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Autores principales:	Portal, Daniel, Espinosa, Joaquín Maximiliano, Lobo, Guillermo Sebastián, Kadener, Sebastián, Pereira, Claudio Alejandro, de la Mata, Manuel, Kornblihtt, Alberto Rodolfo, Baralle, Francisco Ernesto, Flawiá, Mirtha María, Torres, Héctor Norberto
Publicado:	2003
Materias:	cis-Splicing Evolution SR protein trans-Splicing Trypanosoma cruzi arginine fibronectin isoprotein peptide protein kinase protein Prp2 protozoal protein ribonucleoprotein serine serine arginine protein serine arginine protein kinase splicing factor 2 unclassified drug amino acid sequence amino terminal sequence article assay controlled study enhancer region eukaryote evolution exon fungus mutant gene overexpression HeLa cell human human cell in vivo study molecular evolution nonhuman nucleotide sequence phenotype priority journal protein family protein phosphorylation RNA splicing Schizosaccharomyces pombe trans splicing wild type yeast Amino Acid Sequence Animals Arginine Evolution, Molecular Hela Cells Humans Protozoan Proteins RNA Splicing Schizosaccharomyces Sequence Alignment Serine Eukaryota Trypanosoma
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v127_n1_p37_Portal http://hdl.handle.net/20.500.12110/paper_01666851_v127_n1_p37_Portal
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_01666851_v127_n1_p37_Portal
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spelling	paper:paper_01666851_v127_n1_p37_Portal2023-06-08T15:15:53Z An early ancestor in the evolution of splicing: A Trypanosoma cruzi serine-arginine-rich protein (TcSR) is functional in cis-splicing Portal, Daniel Espinosa, Joaquín Maximiliano Lobo, Guillermo Sebastián Kadener, Sebastián Pereira, Claudio Alejandro de la Mata, Manuel Kornblihtt, Alberto Rodolfo Baralle, Francisco Ernesto Flawiá, Mirtha María Torres, Héctor Norberto cis-Splicing Evolution SR protein trans-Splicing Trypanosoma cruzi arginine fibronectin isoprotein peptide protein kinase protein Prp2 protozoal protein ribonucleoprotein serine serine arginine protein serine arginine protein kinase splicing factor 2 unclassified drug amino acid sequence amino terminal sequence article assay controlled study enhancer region eukaryote evolution exon fungus mutant gene overexpression HeLa cell human human cell in vivo study molecular evolution nonhuman nucleotide sequence phenotype priority journal protein family protein phosphorylation RNA splicing Schizosaccharomyces pombe trans splicing Trypanosoma cruzi wild type yeast Amino Acid Sequence Animals Arginine Evolution, Molecular Hela Cells Humans Protozoan Proteins RNA Splicing Schizosaccharomyces Sequence Alignment Serine Trypanosoma cruzi Eukaryota Schizosaccharomyces Schizosaccharomyces pombe Trypanosoma Trypanosoma cruzi A novel serine-arginine-rich protein designated TcSR was identified in Trypanosoma cruzi. The deduced amino acid sequence reveals that TcSR is a member of the SR protein family of splicing factors that contains two RNA-binding domains at the N-terminal side and several serine-arginine repeats at the COOH-terminus. Over expression of either TcSR or the human SR-protein associated splicing factor/splicing factor 2 (ASF/SF2) in wild-type Schizosaccharomyces pombe, provoked an elongated phenotype similar to that of fission yeast over expressing the SR-containing splicing factor Prp2, a U2AF65 orthologue. When a double mutant strain lacking two SR protein-specific protein kinases was used, expression of TcSR or human SR ASF/SF2 splicing factor reverted the mutant to a wild-type phenotype. Transient expression of TcSR in HeLa cells stimulated the inclusion of the EDI exon of human fibronectin in an in vivo functional alternative cis-splicing assay. Inclusion was dependent on a splicing enhancer sequence present in the EDI exon. In addition, TcSR and peptides carrying TcSR-RS domain sequences were phosphorylated by a human SR protein kinase. These results indicate that TcSR is a member of the SR splicing network and that some components common to the trans- and cis-splicing machineries evolved from the early origins of the eukaryotic lineage. © 2002 Elsevier Science B.V. All rights reserved. Fil:Portal, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Espinosa, J.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lobo, G.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Kadener, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pereira, C.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:De La Mata, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Kornblihtt, A.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Baralle, F.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Flawiá, M.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Torres, H.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2003 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v127_n1_p37_Portal http://hdl.handle.net/20.500.12110/paper_01666851_v127_n1_p37_Portal
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	cis-Splicing Evolution SR protein trans-Splicing Trypanosoma cruzi arginine fibronectin isoprotein peptide protein kinase protein Prp2 protozoal protein ribonucleoprotein serine serine arginine protein serine arginine protein kinase splicing factor 2 unclassified drug amino acid sequence amino terminal sequence article assay controlled study enhancer region eukaryote evolution exon fungus mutant gene overexpression HeLa cell human human cell in vivo study molecular evolution nonhuman nucleotide sequence phenotype priority journal protein family protein phosphorylation RNA splicing Schizosaccharomyces pombe trans splicing Trypanosoma cruzi wild type yeast Amino Acid Sequence Animals Arginine Evolution, Molecular Hela Cells Humans Protozoan Proteins RNA Splicing Schizosaccharomyces Sequence Alignment Serine Trypanosoma cruzi Eukaryota Schizosaccharomyces Schizosaccharomyces pombe Trypanosoma Trypanosoma cruzi
spellingShingle	cis-Splicing Evolution SR protein trans-Splicing Trypanosoma cruzi arginine fibronectin isoprotein peptide protein kinase protein Prp2 protozoal protein ribonucleoprotein serine serine arginine protein serine arginine protein kinase splicing factor 2 unclassified drug amino acid sequence amino terminal sequence article assay controlled study enhancer region eukaryote evolution exon fungus mutant gene overexpression HeLa cell human human cell in vivo study molecular evolution nonhuman nucleotide sequence phenotype priority journal protein family protein phosphorylation RNA splicing Schizosaccharomyces pombe trans splicing Trypanosoma cruzi wild type yeast Amino Acid Sequence Animals Arginine Evolution, Molecular Hela Cells Humans Protozoan Proteins RNA Splicing Schizosaccharomyces Sequence Alignment Serine Trypanosoma cruzi Eukaryota Schizosaccharomyces Schizosaccharomyces pombe Trypanosoma Trypanosoma cruzi Portal, Daniel Espinosa, Joaquín Maximiliano Lobo, Guillermo Sebastián Kadener, Sebastián Pereira, Claudio Alejandro de la Mata, Manuel Kornblihtt, Alberto Rodolfo Baralle, Francisco Ernesto Flawiá, Mirtha María Torres, Héctor Norberto An early ancestor in the evolution of splicing: A Trypanosoma cruzi serine-arginine-rich protein (TcSR) is functional in cis-splicing
topic_facet	cis-Splicing Evolution SR protein trans-Splicing Trypanosoma cruzi arginine fibronectin isoprotein peptide protein kinase protein Prp2 protozoal protein ribonucleoprotein serine serine arginine protein serine arginine protein kinase splicing factor 2 unclassified drug amino acid sequence amino terminal sequence article assay controlled study enhancer region eukaryote evolution exon fungus mutant gene overexpression HeLa cell human human cell in vivo study molecular evolution nonhuman nucleotide sequence phenotype priority journal protein family protein phosphorylation RNA splicing Schizosaccharomyces pombe trans splicing Trypanosoma cruzi wild type yeast Amino Acid Sequence Animals Arginine Evolution, Molecular Hela Cells Humans Protozoan Proteins RNA Splicing Schizosaccharomyces Sequence Alignment Serine Trypanosoma cruzi Eukaryota Schizosaccharomyces Schizosaccharomyces pombe Trypanosoma Trypanosoma cruzi
description	A novel serine-arginine-rich protein designated TcSR was identified in Trypanosoma cruzi. The deduced amino acid sequence reveals that TcSR is a member of the SR protein family of splicing factors that contains two RNA-binding domains at the N-terminal side and several serine-arginine repeats at the COOH-terminus. Over expression of either TcSR or the human SR-protein associated splicing factor/splicing factor 2 (ASF/SF2) in wild-type Schizosaccharomyces pombe, provoked an elongated phenotype similar to that of fission yeast over expressing the SR-containing splicing factor Prp2, a U2AF65 orthologue. When a double mutant strain lacking two SR protein-specific protein kinases was used, expression of TcSR or human SR ASF/SF2 splicing factor reverted the mutant to a wild-type phenotype. Transient expression of TcSR in HeLa cells stimulated the inclusion of the EDI exon of human fibronectin in an in vivo functional alternative cis-splicing assay. Inclusion was dependent on a splicing enhancer sequence present in the EDI exon. In addition, TcSR and peptides carrying TcSR-RS domain sequences were phosphorylated by a human SR protein kinase. These results indicate that TcSR is a member of the SR splicing network and that some components common to the trans- and cis-splicing machineries evolved from the early origins of the eukaryotic lineage. © 2002 Elsevier Science B.V. All rights reserved.
author	Portal, Daniel Espinosa, Joaquín Maximiliano Lobo, Guillermo Sebastián Kadener, Sebastián Pereira, Claudio Alejandro de la Mata, Manuel Kornblihtt, Alberto Rodolfo Baralle, Francisco Ernesto Flawiá, Mirtha María Torres, Héctor Norberto
author_facet	Portal, Daniel Espinosa, Joaquín Maximiliano Lobo, Guillermo Sebastián Kadener, Sebastián Pereira, Claudio Alejandro de la Mata, Manuel Kornblihtt, Alberto Rodolfo Baralle, Francisco Ernesto Flawiá, Mirtha María Torres, Héctor Norberto
author_sort	Portal, Daniel
title	An early ancestor in the evolution of splicing: A Trypanosoma cruzi serine-arginine-rich protein (TcSR) is functional in cis-splicing
title_short	An early ancestor in the evolution of splicing: A Trypanosoma cruzi serine-arginine-rich protein (TcSR) is functional in cis-splicing
title_full	An early ancestor in the evolution of splicing: A Trypanosoma cruzi serine-arginine-rich protein (TcSR) is functional in cis-splicing
title_fullStr	An early ancestor in the evolution of splicing: A Trypanosoma cruzi serine-arginine-rich protein (TcSR) is functional in cis-splicing
title_full_unstemmed	An early ancestor in the evolution of splicing: A Trypanosoma cruzi serine-arginine-rich protein (TcSR) is functional in cis-splicing
title_sort	early ancestor in the evolution of splicing: a trypanosoma cruzi serine-arginine-rich protein (tcsr) is functional in cis-splicing
publishDate	2003
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v127_n1_p37_Portal http://hdl.handle.net/20.500.12110/paper_01666851_v127_n1_p37_Portal
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An early ancestor in the evolution of splicing: A Trypanosoma cruzi serine-arginine-rich protein (TcSR) is functional in cis-splicing

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