Characterization of the Trypanosoma cruzi Cdc2p-related protein kinase 1 and identification of three novel associating cyclins
Several Cdc2p-related protein kinases (CRKs) have been described in trypanosomatids but their role in the control of the cell cycle nor their biological functions have been addressed. In Trypanosoma cruzi two CRKs have been identified, TzCRK1 and TzCRK3. In this work we further characterize T. cruzi...
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2001
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Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v113_n1_p97_Gomez http://hdl.handle.net/20.500.12110/paper_01666851_v113_n1_p97_Gomez |
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paper:paper_01666851_v113_n1_p97_Gomez2023-06-08T15:15:52Z Characterization of the Trypanosoma cruzi Cdc2p-related protein kinase 1 and identification of three novel associating cyclins Cdc2p-related kinases Cell cycle Cyclins Kinetoplast Trypanosoma cruzi Two-hybrid system cyclin dependent kinase cyclin dependent kinase inhibitor cycline flavopiridol mitochondrial DNA olomoucine protein kinase protein kinase inhibitor article cell cycle cell organelle cellular distribution controlled study enzyme activity enzyme inhibition epimastigote immunoprecipitation nonhuman priority journal protein analysis protein localization protein protein interaction Trypanosoma cruzi Amino Acid Sequence Animals CDC2 Protein Kinase CDC2-CDC28 Kinases Cyclin-Dependent Kinases Cyclins Cytoplasm Enzyme Inhibitors Flavonoids Histones Immunoglobulin G Immunohistochemistry Kinetin Mitochondria Molecular Sequence Data Piperidines Precipitin Tests Protein Kinases Protozoan Proteins Purines Retinoblastoma Protein Sequence Alignment Trypanosoma cruzi Trypanosoma Trypanosoma cruzi Trypanosomatidae Several Cdc2p-related protein kinases (CRKs) have been described in trypanosomatids but their role in the control of the cell cycle nor their biological functions have been addressed. In Trypanosoma cruzi two CRKs have been identified, TzCRK1 and TzCRK3. In this work we further characterize T. cruzi CRK1 and report the identification of three novel associating cyclins. We demonstrate that CRK1 levels and localization do not vary during the cell cycle, and show that it is localized in the cytoplasm, discrete regions of the nucleus, and is highly concentrated in the mitochondrion DNA (kinetoplast), suggesting a putative control function in this organelle. Using purified anti-CRK1 IgGs, we immunoprecipitated from the soluble fraction of T. cruzi epimastigote forms a protein kinase activity which is not inhibited by CDK inhibitors. In addition, we co-precipitated with p13Suc1p beads a kinase activity that was inhibited by the CDK inhibitor flavopiridol and olomoucine. Lastly, using the yeast two-hybrid system we identified three novel cyclin-like proteins able to associate with TzCRK1, and demonstrate that two of these cyclins also bind the T. cruzi CRK3 protein, indicating that these two CRKs are cyclin-dependent kinases. © 2001 Elsevier Science B.V. 2001 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v113_n1_p97_Gomez http://hdl.handle.net/20.500.12110/paper_01666851_v113_n1_p97_Gomez |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
Cdc2p-related kinases Cell cycle Cyclins Kinetoplast Trypanosoma cruzi Two-hybrid system cyclin dependent kinase cyclin dependent kinase inhibitor cycline flavopiridol mitochondrial DNA olomoucine protein kinase protein kinase inhibitor article cell cycle cell organelle cellular distribution controlled study enzyme activity enzyme inhibition epimastigote immunoprecipitation nonhuman priority journal protein analysis protein localization protein protein interaction Trypanosoma cruzi Amino Acid Sequence Animals CDC2 Protein Kinase CDC2-CDC28 Kinases Cyclin-Dependent Kinases Cyclins Cytoplasm Enzyme Inhibitors Flavonoids Histones Immunoglobulin G Immunohistochemistry Kinetin Mitochondria Molecular Sequence Data Piperidines Precipitin Tests Protein Kinases Protozoan Proteins Purines Retinoblastoma Protein Sequence Alignment Trypanosoma cruzi Trypanosoma Trypanosoma cruzi Trypanosomatidae |
spellingShingle |
Cdc2p-related kinases Cell cycle Cyclins Kinetoplast Trypanosoma cruzi Two-hybrid system cyclin dependent kinase cyclin dependent kinase inhibitor cycline flavopiridol mitochondrial DNA olomoucine protein kinase protein kinase inhibitor article cell cycle cell organelle cellular distribution controlled study enzyme activity enzyme inhibition epimastigote immunoprecipitation nonhuman priority journal protein analysis protein localization protein protein interaction Trypanosoma cruzi Amino Acid Sequence Animals CDC2 Protein Kinase CDC2-CDC28 Kinases Cyclin-Dependent Kinases Cyclins Cytoplasm Enzyme Inhibitors Flavonoids Histones Immunoglobulin G Immunohistochemistry Kinetin Mitochondria Molecular Sequence Data Piperidines Precipitin Tests Protein Kinases Protozoan Proteins Purines Retinoblastoma Protein Sequence Alignment Trypanosoma cruzi Trypanosoma Trypanosoma cruzi Trypanosomatidae Characterization of the Trypanosoma cruzi Cdc2p-related protein kinase 1 and identification of three novel associating cyclins |
topic_facet |
Cdc2p-related kinases Cell cycle Cyclins Kinetoplast Trypanosoma cruzi Two-hybrid system cyclin dependent kinase cyclin dependent kinase inhibitor cycline flavopiridol mitochondrial DNA olomoucine protein kinase protein kinase inhibitor article cell cycle cell organelle cellular distribution controlled study enzyme activity enzyme inhibition epimastigote immunoprecipitation nonhuman priority journal protein analysis protein localization protein protein interaction Trypanosoma cruzi Amino Acid Sequence Animals CDC2 Protein Kinase CDC2-CDC28 Kinases Cyclin-Dependent Kinases Cyclins Cytoplasm Enzyme Inhibitors Flavonoids Histones Immunoglobulin G Immunohistochemistry Kinetin Mitochondria Molecular Sequence Data Piperidines Precipitin Tests Protein Kinases Protozoan Proteins Purines Retinoblastoma Protein Sequence Alignment Trypanosoma cruzi Trypanosoma Trypanosoma cruzi Trypanosomatidae |
description |
Several Cdc2p-related protein kinases (CRKs) have been described in trypanosomatids but their role in the control of the cell cycle nor their biological functions have been addressed. In Trypanosoma cruzi two CRKs have been identified, TzCRK1 and TzCRK3. In this work we further characterize T. cruzi CRK1 and report the identification of three novel associating cyclins. We demonstrate that CRK1 levels and localization do not vary during the cell cycle, and show that it is localized in the cytoplasm, discrete regions of the nucleus, and is highly concentrated in the mitochondrion DNA (kinetoplast), suggesting a putative control function in this organelle. Using purified anti-CRK1 IgGs, we immunoprecipitated from the soluble fraction of T. cruzi epimastigote forms a protein kinase activity which is not inhibited by CDK inhibitors. In addition, we co-precipitated with p13Suc1p beads a kinase activity that was inhibited by the CDK inhibitor flavopiridol and olomoucine. Lastly, using the yeast two-hybrid system we identified three novel cyclin-like proteins able to associate with TzCRK1, and demonstrate that two of these cyclins also bind the T. cruzi CRK3 protein, indicating that these two CRKs are cyclin-dependent kinases. © 2001 Elsevier Science B.V. |
title |
Characterization of the Trypanosoma cruzi Cdc2p-related protein kinase 1 and identification of three novel associating cyclins |
title_short |
Characterization of the Trypanosoma cruzi Cdc2p-related protein kinase 1 and identification of three novel associating cyclins |
title_full |
Characterization of the Trypanosoma cruzi Cdc2p-related protein kinase 1 and identification of three novel associating cyclins |
title_fullStr |
Characterization of the Trypanosoma cruzi Cdc2p-related protein kinase 1 and identification of three novel associating cyclins |
title_full_unstemmed |
Characterization of the Trypanosoma cruzi Cdc2p-related protein kinase 1 and identification of three novel associating cyclins |
title_sort |
characterization of the trypanosoma cruzi cdc2p-related protein kinase 1 and identification of three novel associating cyclins |
publishDate |
2001 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v113_n1_p97_Gomez http://hdl.handle.net/20.500.12110/paper_01666851_v113_n1_p97_Gomez |
_version_ |
1768542733078626304 |