Inhibition of cholinesterases and carboxylesterases of two invertebrate species, Biomphalaria glabrata and Lumbriculus variegatus, by the carbamate pesticide carbaryl

In this study, the effects of sublethal concentrations of the carbamate carbaryl on the cholinesterase (ChE) and carboxylesterase (CES) activities present in the oligochaete Lumbriculus variegatus and in the pigmented Biomphalaria glabrata gastropod were investigated. The results showed that ChE act...

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Autores principales:	Kristoff, Gisela, Cochón, Adriana Cristina
Publicado:	2010
Materias:	Biomphalaria glabrata Carbaryl Carboxylesterases Cholinesterases Lumbriculus variegatus 1 naphthyl acetate 2 naphthyl acetate butyric acid 4 nitrophenyl ester butyric acid derivative carbaril carboxylesterase cholinesterase naphthalene derivative unclassified drug annelid carbamate concentration (composition) electrokinesis enzyme gastropod inhibition pesticide sensitivity analysis sublethal effect animal tissue annelid worm article concentration response controlled study ecotoxicity enzyme activity enzyme assay enzyme inhibition enzyme substrate complex IC 50 in vitro study in vivo study lethal dose nonhuman polyacrylamide gel electrophoresis priority journal species comparison species difference tissue homogenate toxicity testing Animals Biomphalaria Carboxylic Ester Hydrolases Enzyme Inhibitors Inhibitory Concentration 50 Oligochaeta Pesticides Time Factors Water Pollutants, Chemical Gastropoda Invertebrata Oligochaeta (Metazoa)
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0166445X_v96_n2_p115_Kristoff http://hdl.handle.net/20.500.12110/paper_0166445X_v96_n2_p115_Kristoff
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_0166445X_v96_n2_p115_Kristoff
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spelling	paper:paper_0166445X_v96_n2_p115_Kristoff2023-06-08T15:15:51Z Inhibition of cholinesterases and carboxylesterases of two invertebrate species, Biomphalaria glabrata and Lumbriculus variegatus, by the carbamate pesticide carbaryl Kristoff, Gisela Cochón, Adriana Cristina Biomphalaria glabrata Carbaryl Carboxylesterases Cholinesterases Lumbriculus variegatus 1 naphthyl acetate 2 naphthyl acetate butyric acid 4 nitrophenyl ester butyric acid derivative carbaril carboxylesterase cholinesterase naphthalene derivative unclassified drug annelid carbamate concentration (composition) electrokinesis enzyme gastropod inhibition pesticide sensitivity analysis sublethal effect animal tissue annelid worm article Biomphalaria glabrata concentration response controlled study ecotoxicity enzyme activity enzyme assay enzyme inhibition enzyme substrate complex IC 50 in vitro study in vivo study lethal dose Lumbriculus variegatus nonhuman polyacrylamide gel electrophoresis priority journal species comparison species difference tissue homogenate toxicity testing Animals Biomphalaria Carbaryl Carboxylic Ester Hydrolases Cholinesterases Enzyme Inhibitors Inhibitory Concentration 50 Oligochaeta Pesticides Time Factors Water Pollutants, Chemical Biomphalaria glabrata Gastropoda Invertebrata Lumbriculus variegatus Oligochaeta (Metazoa) In this study, the effects of sublethal concentrations of the carbamate carbaryl on the cholinesterase (ChE) and carboxylesterase (CES) activities present in the oligochaete Lumbriculus variegatus and in the pigmented Biomphalaria glabrata gastropod were investigated. The results showed that ChE activity from both species was inhibited by in vivo and in vitro exposure to carbaryl, with EC50 and IC50 values approximately 20 times lower for the oligochaete than for the gastropod. On the other hand, the recovery process in uncontaminated media was more efficient in oligochaetes than in snails. Thus, in only 2 h the oligochaetes showed no inhibition with respect to control values whereas the snails did not reach control values even after 48 h of being in pesticide-free water. CES activity was investigated in whole body soft tissue homogenates using three different substrates: p-nitrophenyl butyrate, 1-naphthyl acetate (NA) and 2-NA. In addition, the presence of multiple CES isozymes in L. variegatus and B. glabrata extracts, with activity towards 1- and 2-NA, was confirmed by native polyacrylamide electrophoresis. In both species, the activities measured using the naphthyl substrates were higher than the activity towards p-nitrophenyl butyrate. In addition, B. glabrata showed a higher CES activity than L. variegatus independently of the substrate used. In L. variegatus, in vivo CES activity towards the different substrates was less sensitive to carbaryl inhibition than ChE activity. In contrast, in B. glabrata, CES activity towards p-nitrophenyl butyrate was inhibited at lower insecticide concentrations than ChE. The results of this study contribute to the knowledge of the sensitivity of non-target freshwater invertebrate Type B-esterases towards pesticides. © 2009 Elsevier B.V. All rights reserved. Fil:Kristoff, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cochón, A.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2010 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0166445X_v96_n2_p115_Kristoff http://hdl.handle.net/20.500.12110/paper_0166445X_v96_n2_p115_Kristoff
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Biomphalaria glabrata Carbaryl Carboxylesterases Cholinesterases Lumbriculus variegatus 1 naphthyl acetate 2 naphthyl acetate butyric acid 4 nitrophenyl ester butyric acid derivative carbaril carboxylesterase cholinesterase naphthalene derivative unclassified drug annelid carbamate concentration (composition) electrokinesis enzyme gastropod inhibition pesticide sensitivity analysis sublethal effect animal tissue annelid worm article Biomphalaria glabrata concentration response controlled study ecotoxicity enzyme activity enzyme assay enzyme inhibition enzyme substrate complex IC 50 in vitro study in vivo study lethal dose Lumbriculus variegatus nonhuman polyacrylamide gel electrophoresis priority journal species comparison species difference tissue homogenate toxicity testing Animals Biomphalaria Carbaryl Carboxylic Ester Hydrolases Cholinesterases Enzyme Inhibitors Inhibitory Concentration 50 Oligochaeta Pesticides Time Factors Water Pollutants, Chemical Biomphalaria glabrata Gastropoda Invertebrata Lumbriculus variegatus Oligochaeta (Metazoa)
spellingShingle	Biomphalaria glabrata Carbaryl Carboxylesterases Cholinesterases Lumbriculus variegatus 1 naphthyl acetate 2 naphthyl acetate butyric acid 4 nitrophenyl ester butyric acid derivative carbaril carboxylesterase cholinesterase naphthalene derivative unclassified drug annelid carbamate concentration (composition) electrokinesis enzyme gastropod inhibition pesticide sensitivity analysis sublethal effect animal tissue annelid worm article Biomphalaria glabrata concentration response controlled study ecotoxicity enzyme activity enzyme assay enzyme inhibition enzyme substrate complex IC 50 in vitro study in vivo study lethal dose Lumbriculus variegatus nonhuman polyacrylamide gel electrophoresis priority journal species comparison species difference tissue homogenate toxicity testing Animals Biomphalaria Carbaryl Carboxylic Ester Hydrolases Cholinesterases Enzyme Inhibitors Inhibitory Concentration 50 Oligochaeta Pesticides Time Factors Water Pollutants, Chemical Biomphalaria glabrata Gastropoda Invertebrata Lumbriculus variegatus Oligochaeta (Metazoa) Kristoff, Gisela Cochón, Adriana Cristina Inhibition of cholinesterases and carboxylesterases of two invertebrate species, Biomphalaria glabrata and Lumbriculus variegatus, by the carbamate pesticide carbaryl
topic_facet	Biomphalaria glabrata Carbaryl Carboxylesterases Cholinesterases Lumbriculus variegatus 1 naphthyl acetate 2 naphthyl acetate butyric acid 4 nitrophenyl ester butyric acid derivative carbaril carboxylesterase cholinesterase naphthalene derivative unclassified drug annelid carbamate concentration (composition) electrokinesis enzyme gastropod inhibition pesticide sensitivity analysis sublethal effect animal tissue annelid worm article Biomphalaria glabrata concentration response controlled study ecotoxicity enzyme activity enzyme assay enzyme inhibition enzyme substrate complex IC 50 in vitro study in vivo study lethal dose Lumbriculus variegatus nonhuman polyacrylamide gel electrophoresis priority journal species comparison species difference tissue homogenate toxicity testing Animals Biomphalaria Carbaryl Carboxylic Ester Hydrolases Cholinesterases Enzyme Inhibitors Inhibitory Concentration 50 Oligochaeta Pesticides Time Factors Water Pollutants, Chemical Biomphalaria glabrata Gastropoda Invertebrata Lumbriculus variegatus Oligochaeta (Metazoa)
description	In this study, the effects of sublethal concentrations of the carbamate carbaryl on the cholinesterase (ChE) and carboxylesterase (CES) activities present in the oligochaete Lumbriculus variegatus and in the pigmented Biomphalaria glabrata gastropod were investigated. The results showed that ChE activity from both species was inhibited by in vivo and in vitro exposure to carbaryl, with EC50 and IC50 values approximately 20 times lower for the oligochaete than for the gastropod. On the other hand, the recovery process in uncontaminated media was more efficient in oligochaetes than in snails. Thus, in only 2 h the oligochaetes showed no inhibition with respect to control values whereas the snails did not reach control values even after 48 h of being in pesticide-free water. CES activity was investigated in whole body soft tissue homogenates using three different substrates: p-nitrophenyl butyrate, 1-naphthyl acetate (NA) and 2-NA. In addition, the presence of multiple CES isozymes in L. variegatus and B. glabrata extracts, with activity towards 1- and 2-NA, was confirmed by native polyacrylamide electrophoresis. In both species, the activities measured using the naphthyl substrates were higher than the activity towards p-nitrophenyl butyrate. In addition, B. glabrata showed a higher CES activity than L. variegatus independently of the substrate used. In L. variegatus, in vivo CES activity towards the different substrates was less sensitive to carbaryl inhibition than ChE activity. In contrast, in B. glabrata, CES activity towards p-nitrophenyl butyrate was inhibited at lower insecticide concentrations than ChE. The results of this study contribute to the knowledge of the sensitivity of non-target freshwater invertebrate Type B-esterases towards pesticides. © 2009 Elsevier B.V. All rights reserved.
author	Kristoff, Gisela Cochón, Adriana Cristina
author_facet	Kristoff, Gisela Cochón, Adriana Cristina
author_sort	Kristoff, Gisela
title	Inhibition of cholinesterases and carboxylesterases of two invertebrate species, Biomphalaria glabrata and Lumbriculus variegatus, by the carbamate pesticide carbaryl
title_short	Inhibition of cholinesterases and carboxylesterases of two invertebrate species, Biomphalaria glabrata and Lumbriculus variegatus, by the carbamate pesticide carbaryl
title_full	Inhibition of cholinesterases and carboxylesterases of two invertebrate species, Biomphalaria glabrata and Lumbriculus variegatus, by the carbamate pesticide carbaryl
title_fullStr	Inhibition of cholinesterases and carboxylesterases of two invertebrate species, Biomphalaria glabrata and Lumbriculus variegatus, by the carbamate pesticide carbaryl
title_full_unstemmed	Inhibition of cholinesterases and carboxylesterases of two invertebrate species, Biomphalaria glabrata and Lumbriculus variegatus, by the carbamate pesticide carbaryl
title_sort	inhibition of cholinesterases and carboxylesterases of two invertebrate species, biomphalaria glabrata and lumbriculus variegatus, by the carbamate pesticide carbaryl
publishDate	2010
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0166445X_v96_n2_p115_Kristoff http://hdl.handle.net/20.500.12110/paper_0166445X_v96_n2_p115_Kristoff
work_keys_str_mv	AT kristoffgisela inhibitionofcholinesterasesandcarboxylesterasesoftwoinvertebratespeciesbiomphalariaglabrataandlumbriculusvariegatusbythecarbamatepesticidecarbaryl AT cochonadrianacristina inhibitionofcholinesterasesandcarboxylesterasesoftwoinvertebratespeciesbiomphalariaglabrataandlumbriculusvariegatusbythecarbamatepesticidecarbaryl
_version_	1768544636237774848

Inhibition of cholinesterases and carboxylesterases of two invertebrate species, Biomphalaria glabrata and Lumbriculus variegatus, by the carbamate pesticide carbaryl

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