Vitellogenin detection in surface mucus of the South American cichlid fish Cichlasoma dimerus (Heckel, 1840) induced by estradiol-17β. Effects on liver and gonads
During the last decade, special attention has been focused on the consequences of exposure to environmental estrogens on reproduction in wild fish species. For this reason, characterization of biomarkers of such exposures could result in useful tools for these studies. The detection of vitellogenin...
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2003
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0166445X_v63_n2_p127_Moncaut http://hdl.handle.net/20.500.12110/paper_0166445X_v63_n2_p127_Moncaut |
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paper:paper_0166445X_v63_n2_p127_Moncaut2023-06-08T15:15:49Z Vitellogenin detection in surface mucus of the South American cichlid fish Cichlasoma dimerus (Heckel, 1840) induced by estradiol-17β. Effects on liver and gonads Moncaut, Natalia P. Maggese, María Cristina Irene Cichlasoma dimerus Cichlids Estradiol Liver and testes pathology Mucus Vitellogenin antiserum biological marker estradiol protein vitellogenin biomarker estrogenic compound fish pollution exposure reproduction animal cell animal experiment animal tissue article bioaccumulation cell function cell maturation cell structure cichlid controlled study dot hybridization environmental exposure estrogen activity female freshwater environment germ cell histopathology immunocytochemistry immunoreactivity lipogenesis liver liver cell male molecular weight mucus nonhuman plasma priority journal protein synthesis spermatogenesis testis Western blotting Animals Biological Markers Cichlids Environmental Exposure Estradiol Female Gonads Immunohistochemistry Liver Male Mucus Vitellogenins South America Cichlasoma Cichlidae Perciformes Teleostei During the last decade, special attention has been focused on the consequences of exposure to environmental estrogens on reproduction in wild fish species. For this reason, characterization of biomarkers of such exposures could result in useful tools for these studies. The detection of vitellogenin (Vtg), a precursor of yolk proteins, is being intensely studied since its synthesis in the liver is regulated by the estradiol-17β and is influenced by other estrogenic compounds. The aim of this work was to assess the presence of Vtg in the surface mucus of males of Cichlasoma dimerus (Teleostei, Perciformes), a typical South American freshwater cichlid, after hormonal treatment with estradiol-17β (intraperitoneal injections of 10 μg E 2 /g fish). Plasma and mucus from females and treated males analyzed by Western blot revealed that different heterologous antisera against Vtg bind to putative protein of 180 & 120 kDa and 120 & 110 kDa, respectively, whereas no reaction was found in samples of untreated males. The same profile was observed in mucus samples using Dot blot, a very easy and direct technique. Using immunocytochemistry techniques, immunoreactive Vtg (ir-Vtg) producing cells in the liver of females and treated males were detected. Testes and liver tissues were also assessed by histological techniques. Marked alterations in both organs were observed, such as lower sperm production, presence of immature germ cells in the lobular lumen of testes, and some morphology changes in the hepatocytes due to the accumulation of Vtg. This is the first report about the effects of an estrogen in the Vtg synthesis and their consequences on liver and gonads of a South American fresh water cichlid. These results also support the possibility of using Vtg from surface mucus as a potential biomarker for estrogenic compounds through a noninvasive, useful and easy assay to monitor the presence of endocrine disrupting chemicals in the environment. © 2002 Elsevier Science B.V. All rights reserved. Fil:Moncaut, N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Maggese, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2003 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0166445X_v63_n2_p127_Moncaut http://hdl.handle.net/20.500.12110/paper_0166445X_v63_n2_p127_Moncaut |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Cichlasoma dimerus Cichlids Estradiol Liver and testes pathology Mucus Vitellogenin antiserum biological marker estradiol protein vitellogenin biomarker estrogenic compound fish pollution exposure reproduction animal cell animal experiment animal tissue article bioaccumulation cell function cell maturation cell structure cichlid controlled study dot hybridization environmental exposure estrogen activity female freshwater environment germ cell histopathology immunocytochemistry immunoreactivity lipogenesis liver liver cell male molecular weight mucus nonhuman plasma priority journal protein synthesis spermatogenesis testis Western blotting Animals Biological Markers Cichlids Environmental Exposure Estradiol Female Gonads Immunohistochemistry Liver Male Mucus Vitellogenins South America Cichlasoma Cichlidae Perciformes Teleostei |
| spellingShingle |
Cichlasoma dimerus Cichlids Estradiol Liver and testes pathology Mucus Vitellogenin antiserum biological marker estradiol protein vitellogenin biomarker estrogenic compound fish pollution exposure reproduction animal cell animal experiment animal tissue article bioaccumulation cell function cell maturation cell structure cichlid controlled study dot hybridization environmental exposure estrogen activity female freshwater environment germ cell histopathology immunocytochemistry immunoreactivity lipogenesis liver liver cell male molecular weight mucus nonhuman plasma priority journal protein synthesis spermatogenesis testis Western blotting Animals Biological Markers Cichlids Environmental Exposure Estradiol Female Gonads Immunohistochemistry Liver Male Mucus Vitellogenins South America Cichlasoma Cichlidae Perciformes Teleostei Moncaut, Natalia P. Maggese, María Cristina Irene Vitellogenin detection in surface mucus of the South American cichlid fish Cichlasoma dimerus (Heckel, 1840) induced by estradiol-17β. Effects on liver and gonads |
| topic_facet |
Cichlasoma dimerus Cichlids Estradiol Liver and testes pathology Mucus Vitellogenin antiserum biological marker estradiol protein vitellogenin biomarker estrogenic compound fish pollution exposure reproduction animal cell animal experiment animal tissue article bioaccumulation cell function cell maturation cell structure cichlid controlled study dot hybridization environmental exposure estrogen activity female freshwater environment germ cell histopathology immunocytochemistry immunoreactivity lipogenesis liver liver cell male molecular weight mucus nonhuman plasma priority journal protein synthesis spermatogenesis testis Western blotting Animals Biological Markers Cichlids Environmental Exposure Estradiol Female Gonads Immunohistochemistry Liver Male Mucus Vitellogenins South America Cichlasoma Cichlidae Perciformes Teleostei |
| description |
During the last decade, special attention has been focused on the consequences of exposure to environmental estrogens on reproduction in wild fish species. For this reason, characterization of biomarkers of such exposures could result in useful tools for these studies. The detection of vitellogenin (Vtg), a precursor of yolk proteins, is being intensely studied since its synthesis in the liver is regulated by the estradiol-17β and is influenced by other estrogenic compounds. The aim of this work was to assess the presence of Vtg in the surface mucus of males of Cichlasoma dimerus (Teleostei, Perciformes), a typical South American freshwater cichlid, after hormonal treatment with estradiol-17β (intraperitoneal injections of 10 μg E 2 /g fish). Plasma and mucus from females and treated males analyzed by Western blot revealed that different heterologous antisera against Vtg bind to putative protein of 180 & 120 kDa and 120 & 110 kDa, respectively, whereas no reaction was found in samples of untreated males. The same profile was observed in mucus samples using Dot blot, a very easy and direct technique. Using immunocytochemistry techniques, immunoreactive Vtg (ir-Vtg) producing cells in the liver of females and treated males were detected. Testes and liver tissues were also assessed by histological techniques. Marked alterations in both organs were observed, such as lower sperm production, presence of immature germ cells in the lobular lumen of testes, and some morphology changes in the hepatocytes due to the accumulation of Vtg. This is the first report about the effects of an estrogen in the Vtg synthesis and their consequences on liver and gonads of a South American fresh water cichlid. These results also support the possibility of using Vtg from surface mucus as a potential biomarker for estrogenic compounds through a noninvasive, useful and easy assay to monitor the presence of endocrine disrupting chemicals in the environment. © 2002 Elsevier Science B.V. All rights reserved. |
| author |
Moncaut, Natalia P. Maggese, María Cristina Irene |
| author_facet |
Moncaut, Natalia P. Maggese, María Cristina Irene |
| author_sort |
Moncaut, Natalia P. |
| title |
Vitellogenin detection in surface mucus of the South American cichlid fish Cichlasoma dimerus (Heckel, 1840) induced by estradiol-17β. Effects on liver and gonads |
| title_short |
Vitellogenin detection in surface mucus of the South American cichlid fish Cichlasoma dimerus (Heckel, 1840) induced by estradiol-17β. Effects on liver and gonads |
| title_full |
Vitellogenin detection in surface mucus of the South American cichlid fish Cichlasoma dimerus (Heckel, 1840) induced by estradiol-17β. Effects on liver and gonads |
| title_fullStr |
Vitellogenin detection in surface mucus of the South American cichlid fish Cichlasoma dimerus (Heckel, 1840) induced by estradiol-17β. Effects on liver and gonads |
| title_full_unstemmed |
Vitellogenin detection in surface mucus of the South American cichlid fish Cichlasoma dimerus (Heckel, 1840) induced by estradiol-17β. Effects on liver and gonads |
| title_sort |
vitellogenin detection in surface mucus of the south american cichlid fish cichlasoma dimerus (heckel, 1840) induced by estradiol-17β. effects on liver and gonads |
| publishDate |
2003 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0166445X_v63_n2_p127_Moncaut http://hdl.handle.net/20.500.12110/paper_0166445X_v63_n2_p127_Moncaut |
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AT moncautnataliap vitellogenindetectioninsurfacemucusofthesouthamericancichlidfishcichlasomadimerusheckel1840inducedbyestradiol17beffectsonliverandgonads AT maggesemariacristinairene vitellogenindetectioninsurfacemucusofthesouthamericancichlidfishcichlasomadimerusheckel1840inducedbyestradiol17beffectsonliverandgonads |
| _version_ |
1768543841296580608 |