Effects of atrazine on vitellogenesis, steroid levels and lipid peroxidation, in female red swamp crayfish Procambarus clarkii
Atrazine, a widely use herbicide, has been classified as a potential endocrine disruptor, especially for freshwater species. In this study, we tested the hypothesis that atrazine can affect reproduction in crayfish through dysregulation of vitellogenin expression and hormone synthesis. Adult female...
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2018
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0166445X_v197_n_p136_Silveyra http://hdl.handle.net/20.500.12110/paper_0166445X_v197_n_p136_Silveyra |
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paper:paper_0166445X_v197_n_p136_Silveyra2023-06-08T15:15:49Z Effects of atrazine on vitellogenesis, steroid levels and lipid peroxidation, in female red swamp crayfish Procambarus clarkii Atrazine Crayfish Lipid peroxidation Ovary Sexual steroids Vitellogenin atrazine glutathione steroid thiobarbituric acid reactive substance vitellogenin atrazine estradiol glucose herbicide lactic acid messenger RNA steroid testosterone thiobarbituric acid reactive substance vitellogenin atrazine crayfish endocrine disruptor histology hormone lipid pollution effect pollution exposure reproductive biology steroid animal tissue Article controlled study environmental exposure female hemolymph histopathology hormone synthesis lipid peroxidation metabolic stress nonhuman priority journal Procambarus clarkii protein content protein expression reproductive toxicity survival analysis toxicity testing vitellogenesis animal crayfish drug effects gene expression regulation genetics hepatopancreas lipid peroxidation metabolism metabolome oocyte ovary reproducibility reproduction toxicity vitellogenesis water pollutant Astacoidea Procambarus clarkii Animals Astacoidea Atrazine Estradiol Female Gene Expression Regulation Glucose Hemolymph Hepatopancreas Herbicides Lactic Acid Lipid Peroxidation Metabolome Oocytes Ovary Reproducibility of Results Reproduction RNA, Messenger Steroids Survival Analysis Testosterone Thiobarbituric Acid Reactive Substances Vitellogenesis Vitellogenins Water Pollutants, Chemical Atrazine, a widely use herbicide, has been classified as a potential endocrine disruptor, especially for freshwater species. In this study, we tested the hypothesis that atrazine can affect reproduction in crayfish through dysregulation of vitellogenin expression and hormone synthesis. Adult female crayfish (Procambarus clarkii) were exposed during one month to atrazine at concentrations of either 1 or 5 mg/L. At the end of the exposure, ovaries, hepatopancreas, and hemolymph samples were harvested for analysis of vitellogenin expression and steroid hormone levels. Ovarian tissue was also sampled for both biochemical and histological analyses. Our results show that atrazine-exposed crayfish had a lower expression of vitellogenin in the ovary and hepatopancreas, as well as smaller oocytes, and reduced vitellogenin content in the ovary. Despite these effects, circulating levels of estradiol increased in females exposed to 5 mg/L of atrazine, showing that the inhibiting effect of atrazine on vitellogenin production was not related to a lower secretion of sexual steroids. Instead, some early stimulating effects of estradiol on vitellogenesis could have occurred, particularly in the hepatopancreas. On the other hand, atrazine caused a higher metabolic effort, in terms of lactate production, presumably triggered to provide the energy needed to face the unspecific stress produced by the herbicide. Lipid peroxidation was not affected by atrazine, but glutathione levels were significantly increased. © 2018 Elsevier B.V. 2018 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0166445X_v197_n_p136_Silveyra http://hdl.handle.net/20.500.12110/paper_0166445X_v197_n_p136_Silveyra |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Atrazine Crayfish Lipid peroxidation Ovary Sexual steroids Vitellogenin atrazine glutathione steroid thiobarbituric acid reactive substance vitellogenin atrazine estradiol glucose herbicide lactic acid messenger RNA steroid testosterone thiobarbituric acid reactive substance vitellogenin atrazine crayfish endocrine disruptor histology hormone lipid pollution effect pollution exposure reproductive biology steroid animal tissue Article controlled study environmental exposure female hemolymph histopathology hormone synthesis lipid peroxidation metabolic stress nonhuman priority journal Procambarus clarkii protein content protein expression reproductive toxicity survival analysis toxicity testing vitellogenesis animal crayfish drug effects gene expression regulation genetics hepatopancreas lipid peroxidation metabolism metabolome oocyte ovary reproducibility reproduction toxicity vitellogenesis water pollutant Astacoidea Procambarus clarkii Animals Astacoidea Atrazine Estradiol Female Gene Expression Regulation Glucose Hemolymph Hepatopancreas Herbicides Lactic Acid Lipid Peroxidation Metabolome Oocytes Ovary Reproducibility of Results Reproduction RNA, Messenger Steroids Survival Analysis Testosterone Thiobarbituric Acid Reactive Substances Vitellogenesis Vitellogenins Water Pollutants, Chemical |
| spellingShingle |
Atrazine Crayfish Lipid peroxidation Ovary Sexual steroids Vitellogenin atrazine glutathione steroid thiobarbituric acid reactive substance vitellogenin atrazine estradiol glucose herbicide lactic acid messenger RNA steroid testosterone thiobarbituric acid reactive substance vitellogenin atrazine crayfish endocrine disruptor histology hormone lipid pollution effect pollution exposure reproductive biology steroid animal tissue Article controlled study environmental exposure female hemolymph histopathology hormone synthesis lipid peroxidation metabolic stress nonhuman priority journal Procambarus clarkii protein content protein expression reproductive toxicity survival analysis toxicity testing vitellogenesis animal crayfish drug effects gene expression regulation genetics hepatopancreas lipid peroxidation metabolism metabolome oocyte ovary reproducibility reproduction toxicity vitellogenesis water pollutant Astacoidea Procambarus clarkii Animals Astacoidea Atrazine Estradiol Female Gene Expression Regulation Glucose Hemolymph Hepatopancreas Herbicides Lactic Acid Lipid Peroxidation Metabolome Oocytes Ovary Reproducibility of Results Reproduction RNA, Messenger Steroids Survival Analysis Testosterone Thiobarbituric Acid Reactive Substances Vitellogenesis Vitellogenins Water Pollutants, Chemical Effects of atrazine on vitellogenesis, steroid levels and lipid peroxidation, in female red swamp crayfish Procambarus clarkii |
| topic_facet |
Atrazine Crayfish Lipid peroxidation Ovary Sexual steroids Vitellogenin atrazine glutathione steroid thiobarbituric acid reactive substance vitellogenin atrazine estradiol glucose herbicide lactic acid messenger RNA steroid testosterone thiobarbituric acid reactive substance vitellogenin atrazine crayfish endocrine disruptor histology hormone lipid pollution effect pollution exposure reproductive biology steroid animal tissue Article controlled study environmental exposure female hemolymph histopathology hormone synthesis lipid peroxidation metabolic stress nonhuman priority journal Procambarus clarkii protein content protein expression reproductive toxicity survival analysis toxicity testing vitellogenesis animal crayfish drug effects gene expression regulation genetics hepatopancreas lipid peroxidation metabolism metabolome oocyte ovary reproducibility reproduction toxicity vitellogenesis water pollutant Astacoidea Procambarus clarkii Animals Astacoidea Atrazine Estradiol Female Gene Expression Regulation Glucose Hemolymph Hepatopancreas Herbicides Lactic Acid Lipid Peroxidation Metabolome Oocytes Ovary Reproducibility of Results Reproduction RNA, Messenger Steroids Survival Analysis Testosterone Thiobarbituric Acid Reactive Substances Vitellogenesis Vitellogenins Water Pollutants, Chemical |
| description |
Atrazine, a widely use herbicide, has been classified as a potential endocrine disruptor, especially for freshwater species. In this study, we tested the hypothesis that atrazine can affect reproduction in crayfish through dysregulation of vitellogenin expression and hormone synthesis. Adult female crayfish (Procambarus clarkii) were exposed during one month to atrazine at concentrations of either 1 or 5 mg/L. At the end of the exposure, ovaries, hepatopancreas, and hemolymph samples were harvested for analysis of vitellogenin expression and steroid hormone levels. Ovarian tissue was also sampled for both biochemical and histological analyses. Our results show that atrazine-exposed crayfish had a lower expression of vitellogenin in the ovary and hepatopancreas, as well as smaller oocytes, and reduced vitellogenin content in the ovary. Despite these effects, circulating levels of estradiol increased in females exposed to 5 mg/L of atrazine, showing that the inhibiting effect of atrazine on vitellogenin production was not related to a lower secretion of sexual steroids. Instead, some early stimulating effects of estradiol on vitellogenesis could have occurred, particularly in the hepatopancreas. On the other hand, atrazine caused a higher metabolic effort, in terms of lactate production, presumably triggered to provide the energy needed to face the unspecific stress produced by the herbicide. Lipid peroxidation was not affected by atrazine, but glutathione levels were significantly increased. © 2018 Elsevier B.V. |
| title |
Effects of atrazine on vitellogenesis, steroid levels and lipid peroxidation, in female red swamp crayfish Procambarus clarkii |
| title_short |
Effects of atrazine on vitellogenesis, steroid levels and lipid peroxidation, in female red swamp crayfish Procambarus clarkii |
| title_full |
Effects of atrazine on vitellogenesis, steroid levels and lipid peroxidation, in female red swamp crayfish Procambarus clarkii |
| title_fullStr |
Effects of atrazine on vitellogenesis, steroid levels and lipid peroxidation, in female red swamp crayfish Procambarus clarkii |
| title_full_unstemmed |
Effects of atrazine on vitellogenesis, steroid levels and lipid peroxidation, in female red swamp crayfish Procambarus clarkii |
| title_sort |
effects of atrazine on vitellogenesis, steroid levels and lipid peroxidation, in female red swamp crayfish procambarus clarkii |
| publishDate |
2018 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0166445X_v197_n_p136_Silveyra http://hdl.handle.net/20.500.12110/paper_0166445X_v197_n_p136_Silveyra |
| _version_ |
1768544590578581504 |