In vitro and in vivo studies of cholinesterases and carboxylesterases in Planorbarius corneus exposed to a phosphorodithioate insecticide: Finding the most sensitive combination of enzymes, substrates, tissues and recovery capacity
Organophosphate insecticides (OPs) continue to be an important class of agrochemicals used in modern agriculture worldwide. Even though these pesticides persist in the environment for a relatively short time, they show a high acute toxicity that may represent a serious hazard for wildlife. Sub-letha...
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2016
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0166445X_v180_n_p186_Otero http://hdl.handle.net/20.500.12110/paper_0166445X_v180_n_p186_Otero |
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paper:paper_0166445X_v180_n_p186_Otero |
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dspace |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Azinphos-methyl B-esterases Biomarkers Freshwater invertebrates 1 naphthyl acetate 2 naphthyl acetate 4 nitrophenyl acetate 4 nitrophenyl butyrate acetic acid derivative acetylthiocholine azinphos methyl butyric acid derivative butyrylthiocholine carboxylesterase choline derivative cholinesterase naphthalene derivative physostigmine propionylthiocholine unclassified drug 4-nitrophenyl acetate 4-nitrophenyl butyrate azinphos methyl biological marker carboxylesterase cholinesterase insecticide nitrophenol organophosphorus compound water pollutant biomarker enzyme activity freshwater ecosystem inhibition insecticide invertebrate organophosphorus pesticide pest control substrate animal experiment animal tissue Article controlled study ecotoxicology environmental exposure enzyme activity enzyme analysis enzyme inhibition enzyme kinetics enzyme substrate complex exocrine gland foot freshwater species gastropod gonad head in vitro study in vivo study lung nonhuman Planorbarius corneus priority journal sensitivity analysis soft tissue tissue specificity water contamination animal antagonists and inhibitors chemistry drug effects enzyme specificity enzymology IC50 kinetics metabolism snail toxicity water pollutant Gastropoda Invertebrata Planorbarius corneus Vertebrata Animals Azinphosmethyl Biomarkers Butyrates Carboxylic Ester Hydrolases Cholinesterases Inhibitory Concentration 50 Insecticides Kinetics Nitrophenols Organophosphorus Compounds Snails Substrate Specificity Water Pollutants, Chemical |
| spellingShingle |
Azinphos-methyl B-esterases Biomarkers Freshwater invertebrates 1 naphthyl acetate 2 naphthyl acetate 4 nitrophenyl acetate 4 nitrophenyl butyrate acetic acid derivative acetylthiocholine azinphos methyl butyric acid derivative butyrylthiocholine carboxylesterase choline derivative cholinesterase naphthalene derivative physostigmine propionylthiocholine unclassified drug 4-nitrophenyl acetate 4-nitrophenyl butyrate azinphos methyl biological marker carboxylesterase cholinesterase insecticide nitrophenol organophosphorus compound water pollutant biomarker enzyme activity freshwater ecosystem inhibition insecticide invertebrate organophosphorus pesticide pest control substrate animal experiment animal tissue Article controlled study ecotoxicology environmental exposure enzyme activity enzyme analysis enzyme inhibition enzyme kinetics enzyme substrate complex exocrine gland foot freshwater species gastropod gonad head in vitro study in vivo study lung nonhuman Planorbarius corneus priority journal sensitivity analysis soft tissue tissue specificity water contamination animal antagonists and inhibitors chemistry drug effects enzyme specificity enzymology IC50 kinetics metabolism snail toxicity water pollutant Gastropoda Invertebrata Planorbarius corneus Vertebrata Animals Azinphosmethyl Biomarkers Butyrates Carboxylic Ester Hydrolases Cholinesterases Inhibitory Concentration 50 Insecticides Kinetics Nitrophenols Organophosphorus Compounds Snails Substrate Specificity Water Pollutants, Chemical Kristoff, Gisela In vitro and in vivo studies of cholinesterases and carboxylesterases in Planorbarius corneus exposed to a phosphorodithioate insecticide: Finding the most sensitive combination of enzymes, substrates, tissues and recovery capacity |
| topic_facet |
Azinphos-methyl B-esterases Biomarkers Freshwater invertebrates 1 naphthyl acetate 2 naphthyl acetate 4 nitrophenyl acetate 4 nitrophenyl butyrate acetic acid derivative acetylthiocholine azinphos methyl butyric acid derivative butyrylthiocholine carboxylesterase choline derivative cholinesterase naphthalene derivative physostigmine propionylthiocholine unclassified drug 4-nitrophenyl acetate 4-nitrophenyl butyrate azinphos methyl biological marker carboxylesterase cholinesterase insecticide nitrophenol organophosphorus compound water pollutant biomarker enzyme activity freshwater ecosystem inhibition insecticide invertebrate organophosphorus pesticide pest control substrate animal experiment animal tissue Article controlled study ecotoxicology environmental exposure enzyme activity enzyme analysis enzyme inhibition enzyme kinetics enzyme substrate complex exocrine gland foot freshwater species gastropod gonad head in vitro study in vivo study lung nonhuman Planorbarius corneus priority journal sensitivity analysis soft tissue tissue specificity water contamination animal antagonists and inhibitors chemistry drug effects enzyme specificity enzymology IC50 kinetics metabolism snail toxicity water pollutant Gastropoda Invertebrata Planorbarius corneus Vertebrata Animals Azinphosmethyl Biomarkers Butyrates Carboxylic Ester Hydrolases Cholinesterases Inhibitory Concentration 50 Insecticides Kinetics Nitrophenols Organophosphorus Compounds Snails Substrate Specificity Water Pollutants, Chemical |
| description |
Organophosphate insecticides (OPs) continue to be an important class of agrochemicals used in modern agriculture worldwide. Even though these pesticides persist in the environment for a relatively short time, they show a high acute toxicity that may represent a serious hazard for wildlife. Sub-lethal effects on non-target species are a focus in pest management programs and should be used as biomarkers. Cholinesterases (ChEs) are the most used biomarker of OP exposure in vertebrate and invertebrate species. However, the combined monitoring of ChE and carboxylesterase (CE) activities may provide a more useful indication of exposure and effect of the organisms. The objective of the present work was to find the most sensitive combination of enzyme, substrate, tissue and capacity to recovery of B-esterases in the freshwater gastropod Planorbarius corneus exposed to the OP azinphos-methyl. For this purpose, ChE and CE activities in different tissues of P. corneus (head-foot, pulmonary region, digestive gland, gonads and whole organism soft tissue) were studied. Measurements of ChE activity were performed using three substrates: acetylthiocholine, propionylthiocholine and butyrylthiocholine and CE activity using four different substrates: p-nitrophenyl acetate, p-nitrophenyl butyrate, 1-naphthyl acetate, and 2-naphthyl acetate in control and exposed organisms. Finally, the recovery rates of ChE and CE activities following 48 h exposure to azinphos-methyl were analyzed. Our results show a preference for acetylthiocholine as substrate, a high inhibition with eserine (a selective ChE inhibitor) and inhibition with excess of substrate in all the analyzed tissues. The highest ChE and CE activity was found in the pulmonary region and in the digestive gland, respectively. The highest CE Vmax was obtained with 1 and 2-naphthyl acetate in all the tissues. CEs were more sensitive than ChE to azinphos-methyl exposure. The highest sensitivity was found using p-nitrophenyl acetate and butyrate as substrates. On the other hand, CEs of the digestive gland and the pulmonary region were more sensitive than CEs of the whole organism soft tissue. Regarding the recovery of enzyme activities after 48 h exposure, ChE and CEs with p-nitrophenyl butyrate reached control values after 14 days in the digestive gland and after 21 days in the pulmonary region. Our results show marked differences in P. corneus basal ChE and CE activities depending on substrates and the tissue. Also, both tissue-dependent and substrate-dependent variations in sensitivity to azinphos-methyl exposure and recovery were obtained. CEs measured with p-nitrophenyl butyrate in the pulmonary region were the best combination to be used as biomarker of exposure to azinphos-methyl due to their sensitivity and low recovery capacity. Environmental concentrations of azinphos-methyl inhibited CE activity so they could be used as effective biomarkers of aquatic contamination. © 2016 Elsevier B.V. |
| author |
Kristoff, Gisela |
| author_facet |
Kristoff, Gisela |
| author_sort |
Kristoff, Gisela |
| title |
In vitro and in vivo studies of cholinesterases and carboxylesterases in Planorbarius corneus exposed to a phosphorodithioate insecticide: Finding the most sensitive combination of enzymes, substrates, tissues and recovery capacity |
| title_short |
In vitro and in vivo studies of cholinesterases and carboxylesterases in Planorbarius corneus exposed to a phosphorodithioate insecticide: Finding the most sensitive combination of enzymes, substrates, tissues and recovery capacity |
| title_full |
In vitro and in vivo studies of cholinesterases and carboxylesterases in Planorbarius corneus exposed to a phosphorodithioate insecticide: Finding the most sensitive combination of enzymes, substrates, tissues and recovery capacity |
| title_fullStr |
In vitro and in vivo studies of cholinesterases and carboxylesterases in Planorbarius corneus exposed to a phosphorodithioate insecticide: Finding the most sensitive combination of enzymes, substrates, tissues and recovery capacity |
| title_full_unstemmed |
In vitro and in vivo studies of cholinesterases and carboxylesterases in Planorbarius corneus exposed to a phosphorodithioate insecticide: Finding the most sensitive combination of enzymes, substrates, tissues and recovery capacity |
| title_sort |
in vitro and in vivo studies of cholinesterases and carboxylesterases in planorbarius corneus exposed to a phosphorodithioate insecticide: finding the most sensitive combination of enzymes, substrates, tissues and recovery capacity |
| publishDate |
2016 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0166445X_v180_n_p186_Otero http://hdl.handle.net/20.500.12110/paper_0166445X_v180_n_p186_Otero |
| work_keys_str_mv |
AT kristoffgisela invitroandinvivostudiesofcholinesterasesandcarboxylesterasesinplanorbariuscorneusexposedtoaphosphorodithioateinsecticidefindingthemostsensitivecombinationofenzymessubstratestissuesandrecoverycapacity |
| _version_ |
1768545460996276224 |
| spelling |
paper:paper_0166445X_v180_n_p186_Otero2023-06-08T15:15:49Z In vitro and in vivo studies of cholinesterases and carboxylesterases in Planorbarius corneus exposed to a phosphorodithioate insecticide: Finding the most sensitive combination of enzymes, substrates, tissues and recovery capacity Kristoff, Gisela Azinphos-methyl B-esterases Biomarkers Freshwater invertebrates 1 naphthyl acetate 2 naphthyl acetate 4 nitrophenyl acetate 4 nitrophenyl butyrate acetic acid derivative acetylthiocholine azinphos methyl butyric acid derivative butyrylthiocholine carboxylesterase choline derivative cholinesterase naphthalene derivative physostigmine propionylthiocholine unclassified drug 4-nitrophenyl acetate 4-nitrophenyl butyrate azinphos methyl biological marker carboxylesterase cholinesterase insecticide nitrophenol organophosphorus compound water pollutant biomarker enzyme activity freshwater ecosystem inhibition insecticide invertebrate organophosphorus pesticide pest control substrate animal experiment animal tissue Article controlled study ecotoxicology environmental exposure enzyme activity enzyme analysis enzyme inhibition enzyme kinetics enzyme substrate complex exocrine gland foot freshwater species gastropod gonad head in vitro study in vivo study lung nonhuman Planorbarius corneus priority journal sensitivity analysis soft tissue tissue specificity water contamination animal antagonists and inhibitors chemistry drug effects enzyme specificity enzymology IC50 kinetics metabolism snail toxicity water pollutant Gastropoda Invertebrata Planorbarius corneus Vertebrata Animals Azinphosmethyl Biomarkers Butyrates Carboxylic Ester Hydrolases Cholinesterases Inhibitory Concentration 50 Insecticides Kinetics Nitrophenols Organophosphorus Compounds Snails Substrate Specificity Water Pollutants, Chemical Organophosphate insecticides (OPs) continue to be an important class of agrochemicals used in modern agriculture worldwide. Even though these pesticides persist in the environment for a relatively short time, they show a high acute toxicity that may represent a serious hazard for wildlife. Sub-lethal effects on non-target species are a focus in pest management programs and should be used as biomarkers. Cholinesterases (ChEs) are the most used biomarker of OP exposure in vertebrate and invertebrate species. However, the combined monitoring of ChE and carboxylesterase (CE) activities may provide a more useful indication of exposure and effect of the organisms. The objective of the present work was to find the most sensitive combination of enzyme, substrate, tissue and capacity to recovery of B-esterases in the freshwater gastropod Planorbarius corneus exposed to the OP azinphos-methyl. For this purpose, ChE and CE activities in different tissues of P. corneus (head-foot, pulmonary region, digestive gland, gonads and whole organism soft tissue) were studied. Measurements of ChE activity were performed using three substrates: acetylthiocholine, propionylthiocholine and butyrylthiocholine and CE activity using four different substrates: p-nitrophenyl acetate, p-nitrophenyl butyrate, 1-naphthyl acetate, and 2-naphthyl acetate in control and exposed organisms. Finally, the recovery rates of ChE and CE activities following 48 h exposure to azinphos-methyl were analyzed. Our results show a preference for acetylthiocholine as substrate, a high inhibition with eserine (a selective ChE inhibitor) and inhibition with excess of substrate in all the analyzed tissues. The highest ChE and CE activity was found in the pulmonary region and in the digestive gland, respectively. The highest CE Vmax was obtained with 1 and 2-naphthyl acetate in all the tissues. CEs were more sensitive than ChE to azinphos-methyl exposure. The highest sensitivity was found using p-nitrophenyl acetate and butyrate as substrates. On the other hand, CEs of the digestive gland and the pulmonary region were more sensitive than CEs of the whole organism soft tissue. Regarding the recovery of enzyme activities after 48 h exposure, ChE and CEs with p-nitrophenyl butyrate reached control values after 14 days in the digestive gland and after 21 days in the pulmonary region. Our results show marked differences in P. corneus basal ChE and CE activities depending on substrates and the tissue. Also, both tissue-dependent and substrate-dependent variations in sensitivity to azinphos-methyl exposure and recovery were obtained. CEs measured with p-nitrophenyl butyrate in the pulmonary region were the best combination to be used as biomarker of exposure to azinphos-methyl due to their sensitivity and low recovery capacity. Environmental concentrations of azinphos-methyl inhibited CE activity so they could be used as effective biomarkers of aquatic contamination. © 2016 Elsevier B.V. Fil:Kristoff, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2016 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0166445X_v180_n_p186_Otero http://hdl.handle.net/20.500.12110/paper_0166445X_v180_n_p186_Otero |