Recovery study of cholinesterases and neurotoxic signs in the non-target freshwater invertebrate Chilina gibbosa after an acute exposure to an environmental concentration of azinphos-methyl
Azinphos-methyl belongs to the class of organophosphate insecticides which are recognized for their anticholinesterase action. It is one of the most frequently used insecticides in the Upper Valley of Río Negro and Río Neuquén in Argentina, where agriculture represents the second most important econ...
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| Publicado: |
2015
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0166445X_v167_n_p248_Cossi http://hdl.handle.net/20.500.12110/paper_0166445X_v167_n_p248_Cossi |
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paper:paper_0166445X_v167_n_p248_Cossi |
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| record_format |
dspace |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Azinphos-methyl B-esterases Biomarkers Invertebrates Pesticides azinphos methyl biological marker carboxylesterase cholinesterase azinphos methyl biological marker carboxylesterase cholinesterase cholinesterase inhibitor fresh water insecticide water pollutant azinphos methyl biomarker concentration (composition) ecotoxicology enzyme activity food web gastropod organophosphorus pesticide pollution exposure pollution tolerance toxicity acute toxicity animal experiment aquatic environment Article Chilina gibbosa concentration (parameters) controlled study disease severity environmental exposure enzyme activity enzyme inhibition freshwater species mollusc neurotoxicity nonhuman priority journal sensitivity analysis South America structure activity relation animal Argentina Chile drug effects enzyme activation enzymology metabolism snail toxicity water pollutant Argentina Chile Patagonia Chilina Invertebrata Animals Argentina Azinphosmethyl Biomarkers Carboxylic Ester Hydrolases Chile Cholinesterase Inhibitors Cholinesterases Enzyme Activation Fresh Water Insecticides Snails Water Pollutants, Chemical |
| spellingShingle |
Azinphos-methyl B-esterases Biomarkers Invertebrates Pesticides azinphos methyl biological marker carboxylesterase cholinesterase azinphos methyl biological marker carboxylesterase cholinesterase cholinesterase inhibitor fresh water insecticide water pollutant azinphos methyl biomarker concentration (composition) ecotoxicology enzyme activity food web gastropod organophosphorus pesticide pollution exposure pollution tolerance toxicity acute toxicity animal experiment aquatic environment Article Chilina gibbosa concentration (parameters) controlled study disease severity environmental exposure enzyme activity enzyme inhibition freshwater species mollusc neurotoxicity nonhuman priority journal sensitivity analysis South America structure activity relation animal Argentina Chile drug effects enzyme activation enzymology metabolism snail toxicity water pollutant Argentina Chile Patagonia Chilina Invertebrata Animals Argentina Azinphosmethyl Biomarkers Carboxylic Ester Hydrolases Chile Cholinesterase Inhibitors Cholinesterases Enzyme Activation Fresh Water Insecticides Snails Water Pollutants, Chemical Kristoff, Gisela Recovery study of cholinesterases and neurotoxic signs in the non-target freshwater invertebrate Chilina gibbosa after an acute exposure to an environmental concentration of azinphos-methyl |
| topic_facet |
Azinphos-methyl B-esterases Biomarkers Invertebrates Pesticides azinphos methyl biological marker carboxylesterase cholinesterase azinphos methyl biological marker carboxylesterase cholinesterase cholinesterase inhibitor fresh water insecticide water pollutant azinphos methyl biomarker concentration (composition) ecotoxicology enzyme activity food web gastropod organophosphorus pesticide pollution exposure pollution tolerance toxicity acute toxicity animal experiment aquatic environment Article Chilina gibbosa concentration (parameters) controlled study disease severity environmental exposure enzyme activity enzyme inhibition freshwater species mollusc neurotoxicity nonhuman priority journal sensitivity analysis South America structure activity relation animal Argentina Chile drug effects enzyme activation enzymology metabolism snail toxicity water pollutant Argentina Chile Patagonia Chilina Invertebrata Animals Argentina Azinphosmethyl Biomarkers Carboxylic Ester Hydrolases Chile Cholinesterase Inhibitors Cholinesterases Enzyme Activation Fresh Water Insecticides Snails Water Pollutants, Chemical |
| description |
Azinphos-methyl belongs to the class of organophosphate insecticides which are recognized for their anticholinesterase action. It is one of the most frequently used insecticides in the Upper Valley of Río Negro and Río Neuquén in Argentina, where agriculture represents the second most important economic activity. It has been detected in water from this North Patagonian region throughout the year and the maximum concentration found was 22.48μgL-1 during the application period. Chilina gibbosa is a freshwater gastropod widely distributed in South America, particularly in Patagonia, Argentina and in Southern Chile. Toxicological studies performed with C. gibbosa in our laboratory have reported neurotoxicity signs and cholinesterase inhibition after exposure to azinphos-methyl for 48h. Recovery studies together with characterization of the enzyme and sensitivity of the enzyme to pesticides can improve the toxicological evaluation. However, little is known about recovery patterns in organisms exposed to organophosphates. The aim of the present work was to evaluate the recovery capacity (during 21 days in pesticide-free water) of cholinesterase activity and neurotoxicity in C. gibbosa after 48h of exposure to azinphos-methyl. Also, lethality and carboxylesterase activity were registered during the recovery period. Regarding enzyme activities, after a 48-h exposure to 20μgL-1 of azinphos-methyl, cholinesterases showed an inhibition of 85% with respect to control, while carboxylesterases were not affected. After 21 days in pesticide-free water, cholinesterases continued to be inhibited (70%). Severe neurotoxicity signs were observed after exposure: 82% of the snails presented lack of adherence to vessels, 11% showed weak adherence, and 96% exhibited an abnormal protrusion of the head-foot region from shell. After 21 days in pesticide-free water, only 15% of the snails presented severe signs of neurotoxicity. However, during the recovery period significant lethality (30%) was registered in treated snails. C. gibbosa is a very sensitive organism to azinphos-methyl. These snails play an important role in the structure and function of aquatic food webs in this region. Thus, a decline of this species' population would probably have an impact on aquatic and non-aquatic communities. Our results show that C. gibbosa is a relevant sentinel species for studying exposure and effects of azinphos-methyl using behavioral and biochemical biomarkers. Neurotoxic behavioral signs are very sensitive, non-destructive biomarkers, which can be easily detected for about one week after acute exposure. Cholinesterse activity is a very useful biomarker showing a high sensitivity and a slow recovery capacity increasing the possibility to indirectly detect organophosphates for long periods after a contaminant event. © 2015 Elsevier B.V. |
| author |
Kristoff, Gisela |
| author_facet |
Kristoff, Gisela |
| author_sort |
Kristoff, Gisela |
| title |
Recovery study of cholinesterases and neurotoxic signs in the non-target freshwater invertebrate Chilina gibbosa after an acute exposure to an environmental concentration of azinphos-methyl |
| title_short |
Recovery study of cholinesterases and neurotoxic signs in the non-target freshwater invertebrate Chilina gibbosa after an acute exposure to an environmental concentration of azinphos-methyl |
| title_full |
Recovery study of cholinesterases and neurotoxic signs in the non-target freshwater invertebrate Chilina gibbosa after an acute exposure to an environmental concentration of azinphos-methyl |
| title_fullStr |
Recovery study of cholinesterases and neurotoxic signs in the non-target freshwater invertebrate Chilina gibbosa after an acute exposure to an environmental concentration of azinphos-methyl |
| title_full_unstemmed |
Recovery study of cholinesterases and neurotoxic signs in the non-target freshwater invertebrate Chilina gibbosa after an acute exposure to an environmental concentration of azinphos-methyl |
| title_sort |
recovery study of cholinesterases and neurotoxic signs in the non-target freshwater invertebrate chilina gibbosa after an acute exposure to an environmental concentration of azinphos-methyl |
| publishDate |
2015 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0166445X_v167_n_p248_Cossi http://hdl.handle.net/20.500.12110/paper_0166445X_v167_n_p248_Cossi |
| work_keys_str_mv |
AT kristoffgisela recoverystudyofcholinesterasesandneurotoxicsignsinthenontargetfreshwaterinvertebratechilinagibbosaafteranacuteexposuretoanenvironmentalconcentrationofazinphosmethyl |
| _version_ |
1768546295947984896 |
| spelling |
paper:paper_0166445X_v167_n_p248_Cossi2023-06-08T15:15:48Z Recovery study of cholinesterases and neurotoxic signs in the non-target freshwater invertebrate Chilina gibbosa after an acute exposure to an environmental concentration of azinphos-methyl Kristoff, Gisela Azinphos-methyl B-esterases Biomarkers Invertebrates Pesticides azinphos methyl biological marker carboxylesterase cholinesterase azinphos methyl biological marker carboxylesterase cholinesterase cholinesterase inhibitor fresh water insecticide water pollutant azinphos methyl biomarker concentration (composition) ecotoxicology enzyme activity food web gastropod organophosphorus pesticide pollution exposure pollution tolerance toxicity acute toxicity animal experiment aquatic environment Article Chilina gibbosa concentration (parameters) controlled study disease severity environmental exposure enzyme activity enzyme inhibition freshwater species mollusc neurotoxicity nonhuman priority journal sensitivity analysis South America structure activity relation animal Argentina Chile drug effects enzyme activation enzymology metabolism snail toxicity water pollutant Argentina Chile Patagonia Chilina Invertebrata Animals Argentina Azinphosmethyl Biomarkers Carboxylic Ester Hydrolases Chile Cholinesterase Inhibitors Cholinesterases Enzyme Activation Fresh Water Insecticides Snails Water Pollutants, Chemical Azinphos-methyl belongs to the class of organophosphate insecticides which are recognized for their anticholinesterase action. It is one of the most frequently used insecticides in the Upper Valley of Río Negro and Río Neuquén in Argentina, where agriculture represents the second most important economic activity. It has been detected in water from this North Patagonian region throughout the year and the maximum concentration found was 22.48μgL-1 during the application period. Chilina gibbosa is a freshwater gastropod widely distributed in South America, particularly in Patagonia, Argentina and in Southern Chile. Toxicological studies performed with C. gibbosa in our laboratory have reported neurotoxicity signs and cholinesterase inhibition after exposure to azinphos-methyl for 48h. Recovery studies together with characterization of the enzyme and sensitivity of the enzyme to pesticides can improve the toxicological evaluation. However, little is known about recovery patterns in organisms exposed to organophosphates. The aim of the present work was to evaluate the recovery capacity (during 21 days in pesticide-free water) of cholinesterase activity and neurotoxicity in C. gibbosa after 48h of exposure to azinphos-methyl. Also, lethality and carboxylesterase activity were registered during the recovery period. Regarding enzyme activities, after a 48-h exposure to 20μgL-1 of azinphos-methyl, cholinesterases showed an inhibition of 85% with respect to control, while carboxylesterases were not affected. After 21 days in pesticide-free water, cholinesterases continued to be inhibited (70%). Severe neurotoxicity signs were observed after exposure: 82% of the snails presented lack of adherence to vessels, 11% showed weak adherence, and 96% exhibited an abnormal protrusion of the head-foot region from shell. After 21 days in pesticide-free water, only 15% of the snails presented severe signs of neurotoxicity. However, during the recovery period significant lethality (30%) was registered in treated snails. C. gibbosa is a very sensitive organism to azinphos-methyl. These snails play an important role in the structure and function of aquatic food webs in this region. Thus, a decline of this species' population would probably have an impact on aquatic and non-aquatic communities. Our results show that C. gibbosa is a relevant sentinel species for studying exposure and effects of azinphos-methyl using behavioral and biochemical biomarkers. Neurotoxic behavioral signs are very sensitive, non-destructive biomarkers, which can be easily detected for about one week after acute exposure. Cholinesterse activity is a very useful biomarker showing a high sensitivity and a slow recovery capacity increasing the possibility to indirectly detect organophosphates for long periods after a contaminant event. © 2015 Elsevier B.V. Fil:Kristoff, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2015 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0166445X_v167_n_p248_Cossi http://hdl.handle.net/20.500.12110/paper_0166445X_v167_n_p248_Cossi |