Effects of estradiol and ethinylestradiol on sperm quality, fertilization, and embryo-larval survival of pejerrey fish (Odontesthes bonariensis)
17β-Estradiol (E2) and synthetic 17α-Ethinylestradiol (EE2) are estrogenic compounds present in surface waters as a consequence of municipal sewage discharges. The aim of this study was to evaluate the effects of E2, EE2 and its mixtures on different reproductive parameters and embryo-larval surviva...
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2015
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0166445X_v167_n_p191_Garriz http://hdl.handle.net/20.500.12110/paper_0166445X_v167_n_p191_Garriz |
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paper:paper_0166445X_v167_n_p191_Garriz2023-06-08T15:15:48Z Effects of estradiol and ethinylestradiol on sperm quality, fertilization, and embryo-larval survival of pejerrey fish (Odontesthes bonariensis) Menéndez Helman, Renata Julia Miranda, Leandro Andrés Embryo and larval survival Estradiol Ethinylestradiol Odontesthes bonariensis Sperm quality estradiol ethinylestradiol estradiol ethinylestradiol sewage water pollutant assay concentration (composition) ecotoxicology embryo estrogenic compound fertilization (reproduction) larva sperm survival teleost Argentina Article body movement concentration (parameters) controlled study embryo female fertilization fish hatching lake larva male nonhuman Odontesthes bonariensis priority journal reproductive toxicity sewage disposal sperm quality spermatozoon motility survival rate water contamination animal animal embryo chemistry drug effects fertilization physiology reproduction sewage Smegmamorpha spermatozoon toxicity water pollutant Odontesthes bonariensis Animals Embryo, Nonmammalian Estradiol Ethinyl Estradiol Fertilization Male Reproduction Sewage Smegmamorpha Sperm Motility Spermatozoa Water Pollutants, Chemical 17β-Estradiol (E2) and synthetic 17α-Ethinylestradiol (EE2) are estrogenic compounds present in surface waters as a consequence of municipal sewage discharges. The aim of this study was to evaluate the effects of E2, EE2 and its mixtures on different reproductive parameters and embryo-larval survival in pejerrey fish (Odontesthes bonariensis). In order to analyze the effect of these compounds on sperm quality, fertilization%, embryo-larval survival (%), and the point of no return (PNR), different assays were performed using concentrations 175, 350, 700 and 1400ng/L of E2; 22.5, 45, 90 and 180ng/L of EE2 and mixtures M1 (175 E2+22.5 EE2, ng/L), M2 (350 E2+45 EE2, ng/L), M3 (700 E2+90 EE2, ng/L) and M4 (1400 E2+180 EE2 ng/L). No significant differences in motility parameters were observed between E2 and EE2 treatments and the control group. However, a significant decrease in motility% was recorded for all mixtures tested compared with the control samples. For fertilization%, only sperm activated with M4 showed a significant decrease compared with the control group. In the case of embryo survival, there was only a significant decrease in the highest concentration of EE2 compared with the control group. For the mixtures, M3 is the one that had the most adverse effect on embryo survival. In larval survival, there was a significant decrease in concentration 175 and 700ng/L of E2 compared with the control group. In EE2 treatments, the ones with a significant reduction in larval survival were concentration 45 and 90ng/L. And for the mixture treatments, M1, M3 and M4 had a significantly lower larval survival than the control group. In comparison to other treatments, M1 demonstrated a significant difference in PNR when compared with the control group. The results obtained demonstrated that the exposure to mixtures of E2 and EE2 affected fish sperm motility, fertilization% and, embryo and larval survival even at relevant environmental concentrations highlighting the necessity of considering the effects of pollutants mixtures in ecotoxicological studies. © 2015 Elsevier B.V. Fil:Menéndez-Helman, R.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Miranda, L.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2015 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0166445X_v167_n_p191_Garriz http://hdl.handle.net/20.500.12110/paper_0166445X_v167_n_p191_Garriz |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Embryo and larval survival Estradiol Ethinylestradiol Odontesthes bonariensis Sperm quality estradiol ethinylestradiol estradiol ethinylestradiol sewage water pollutant assay concentration (composition) ecotoxicology embryo estrogenic compound fertilization (reproduction) larva sperm survival teleost Argentina Article body movement concentration (parameters) controlled study embryo female fertilization fish hatching lake larva male nonhuman Odontesthes bonariensis priority journal reproductive toxicity sewage disposal sperm quality spermatozoon motility survival rate water contamination animal animal embryo chemistry drug effects fertilization physiology reproduction sewage Smegmamorpha spermatozoon toxicity water pollutant Odontesthes bonariensis Animals Embryo, Nonmammalian Estradiol Ethinyl Estradiol Fertilization Male Reproduction Sewage Smegmamorpha Sperm Motility Spermatozoa Water Pollutants, Chemical |
| spellingShingle |
Embryo and larval survival Estradiol Ethinylestradiol Odontesthes bonariensis Sperm quality estradiol ethinylestradiol estradiol ethinylestradiol sewage water pollutant assay concentration (composition) ecotoxicology embryo estrogenic compound fertilization (reproduction) larva sperm survival teleost Argentina Article body movement concentration (parameters) controlled study embryo female fertilization fish hatching lake larva male nonhuman Odontesthes bonariensis priority journal reproductive toxicity sewage disposal sperm quality spermatozoon motility survival rate water contamination animal animal embryo chemistry drug effects fertilization physiology reproduction sewage Smegmamorpha spermatozoon toxicity water pollutant Odontesthes bonariensis Animals Embryo, Nonmammalian Estradiol Ethinyl Estradiol Fertilization Male Reproduction Sewage Smegmamorpha Sperm Motility Spermatozoa Water Pollutants, Chemical Menéndez Helman, Renata Julia Miranda, Leandro Andrés Effects of estradiol and ethinylestradiol on sperm quality, fertilization, and embryo-larval survival of pejerrey fish (Odontesthes bonariensis) |
| topic_facet |
Embryo and larval survival Estradiol Ethinylestradiol Odontesthes bonariensis Sperm quality estradiol ethinylestradiol estradiol ethinylestradiol sewage water pollutant assay concentration (composition) ecotoxicology embryo estrogenic compound fertilization (reproduction) larva sperm survival teleost Argentina Article body movement concentration (parameters) controlled study embryo female fertilization fish hatching lake larva male nonhuman Odontesthes bonariensis priority journal reproductive toxicity sewage disposal sperm quality spermatozoon motility survival rate water contamination animal animal embryo chemistry drug effects fertilization physiology reproduction sewage Smegmamorpha spermatozoon toxicity water pollutant Odontesthes bonariensis Animals Embryo, Nonmammalian Estradiol Ethinyl Estradiol Fertilization Male Reproduction Sewage Smegmamorpha Sperm Motility Spermatozoa Water Pollutants, Chemical |
| description |
17β-Estradiol (E2) and synthetic 17α-Ethinylestradiol (EE2) are estrogenic compounds present in surface waters as a consequence of municipal sewage discharges. The aim of this study was to evaluate the effects of E2, EE2 and its mixtures on different reproductive parameters and embryo-larval survival in pejerrey fish (Odontesthes bonariensis). In order to analyze the effect of these compounds on sperm quality, fertilization%, embryo-larval survival (%), and the point of no return (PNR), different assays were performed using concentrations 175, 350, 700 and 1400ng/L of E2; 22.5, 45, 90 and 180ng/L of EE2 and mixtures M1 (175 E2+22.5 EE2, ng/L), M2 (350 E2+45 EE2, ng/L), M3 (700 E2+90 EE2, ng/L) and M4 (1400 E2+180 EE2 ng/L). No significant differences in motility parameters were observed between E2 and EE2 treatments and the control group. However, a significant decrease in motility% was recorded for all mixtures tested compared with the control samples. For fertilization%, only sperm activated with M4 showed a significant decrease compared with the control group. In the case of embryo survival, there was only a significant decrease in the highest concentration of EE2 compared with the control group. For the mixtures, M3 is the one that had the most adverse effect on embryo survival. In larval survival, there was a significant decrease in concentration 175 and 700ng/L of E2 compared with the control group. In EE2 treatments, the ones with a significant reduction in larval survival were concentration 45 and 90ng/L. And for the mixture treatments, M1, M3 and M4 had a significantly lower larval survival than the control group. In comparison to other treatments, M1 demonstrated a significant difference in PNR when compared with the control group. The results obtained demonstrated that the exposure to mixtures of E2 and EE2 affected fish sperm motility, fertilization% and, embryo and larval survival even at relevant environmental concentrations highlighting the necessity of considering the effects of pollutants mixtures in ecotoxicological studies. © 2015 Elsevier B.V. |
| author |
Menéndez Helman, Renata Julia Miranda, Leandro Andrés |
| author_facet |
Menéndez Helman, Renata Julia Miranda, Leandro Andrés |
| author_sort |
Menéndez Helman, Renata Julia |
| title |
Effects of estradiol and ethinylestradiol on sperm quality, fertilization, and embryo-larval survival of pejerrey fish (Odontesthes bonariensis) |
| title_short |
Effects of estradiol and ethinylestradiol on sperm quality, fertilization, and embryo-larval survival of pejerrey fish (Odontesthes bonariensis) |
| title_full |
Effects of estradiol and ethinylestradiol on sperm quality, fertilization, and embryo-larval survival of pejerrey fish (Odontesthes bonariensis) |
| title_fullStr |
Effects of estradiol and ethinylestradiol on sperm quality, fertilization, and embryo-larval survival of pejerrey fish (Odontesthes bonariensis) |
| title_full_unstemmed |
Effects of estradiol and ethinylestradiol on sperm quality, fertilization, and embryo-larval survival of pejerrey fish (Odontesthes bonariensis) |
| title_sort |
effects of estradiol and ethinylestradiol on sperm quality, fertilization, and embryo-larval survival of pejerrey fish (odontesthes bonariensis) |
| publishDate |
2015 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0166445X_v167_n_p191_Garriz http://hdl.handle.net/20.500.12110/paper_0166445X_v167_n_p191_Garriz |
| work_keys_str_mv |
AT menendezhelmanrenatajulia effectsofestradiolandethinylestradiolonspermqualityfertilizationandembryolarvalsurvivalofpejerreyfishodontesthesbonariensis AT mirandaleandroandres effectsofestradiolandethinylestradiolonspermqualityfertilizationandembryolarvalsurvivalofpejerreyfishodontesthesbonariensis |
| _version_ |
1768542687571476480 |