Nonmonotonic response of vitellogenin and estrogen receptor α gene expression after octylphenol exposure of Cichlasoma dimerus (Perciformes, Cichlidae)

In oviparous vertebrates, vitellogenin (VTG) is mainly produced by the liver in response to estrogen (E 2 ) and its synthesis is traditionally coupled to estrogen receptor alpha induction. Even though VTG is a female-specific protein, chemicals that mimic natural estrogens, known as xenoestrogens, c...

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Autores principales:	Genovese, Griselda, Regueira, Mariana, Da Cuña, Rodrigo Hernán, Lo Nostro, Fabiana Laura
Publicado:	2014
Materias:	Cichlid fish Estrogen receptors Nonmonotonic dose-response Octylphenol Vitellogenin gene expression estrogen receptor alpha octylphenol phenol derivative unclassified drug vitellogenin adenosine triphosphatase (potassium sodium) alkylphenol estrogen receptor beta messenger RNA endocrine disruptor water pollutant blood cichlid concentration (composition) cytoplasm dose-response relationship estrogenic compound experimental design gene expression laboratory method phenol protein adult animal experiment animal tissue article basophil Cichlasoma dimerus concentration (parameters) controlled study down regulation environmental exposure eosinophil estrogen receptor alpha gene estrogen receptor beta2 gene female gene expression regulation gene function genetic transcription genotype environment interaction histopathology male nonhuman Perciformes priority journal receptor gene risk management transactivation upregulation vitellogenesis vitellogenin gene water analysis Article basophilia concentration response liver cell mucus nucleotide sequence plasma sperm animal drug effects genetics liver metabolism testis toxicity Cichlasoma Cichlidae Animals Cichlids Endocrine Disruptors Estrogen Receptor alpha Female Gene Expression Regulation Hepatocytes Liver Male Phenols Testis Vitellogenins Water Pollutants, Chemical
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0166445X_v156_n_p30_Genovese http://hdl.handle.net/20.500.12110/paper_0166445X_v156_n_p30_Genovese
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_0166445X_v156_n_p30_Genovese
record_format	dspace
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Cichlid fish Estrogen receptors Nonmonotonic dose-response Octylphenol Vitellogenin gene expression estrogen receptor alpha octylphenol phenol derivative unclassified drug vitellogenin adenosine triphosphatase (potassium sodium) alkylphenol estrogen receptor alpha estrogen receptor beta messenger RNA octylphenol vitellogenin endocrine disruptor estrogen receptor alpha octylphenol phenol derivative vitellogenin water pollutant endocrine disruptor phenol derivative blood cichlid concentration (composition) cytoplasm dose-response relationship endocrine disruptor estrogenic compound experimental design gene expression laboratory method phenol protein adult animal experiment animal tissue article basophil Cichlasoma dimerus concentration (parameters) controlled study down regulation environmental exposure eosinophil estrogen receptor alpha gene estrogen receptor beta2 gene female gene expression regulation gene function genetic transcription genotype environment interaction histopathology male nonhuman Perciformes priority journal receptor gene risk management transactivation upregulation vitellogenesis vitellogenin gene water analysis Article basophilia Cichlasoma dimerus concentration response cytoplasm gene expression liver cell mucus nucleotide sequence Perciformes plasma sperm animal cichlid drug effects genetics liver metabolism testis toxicity water pollutant toxicity Cichlasoma Cichlidae Perciformes Animals Cichlids Endocrine Disruptors Estrogen Receptor alpha Female Gene Expression Regulation Hepatocytes Liver Male Phenols Testis Vitellogenins Water Pollutants, Chemical
spellingShingle	Cichlid fish Estrogen receptors Nonmonotonic dose-response Octylphenol Vitellogenin gene expression estrogen receptor alpha octylphenol phenol derivative unclassified drug vitellogenin adenosine triphosphatase (potassium sodium) alkylphenol estrogen receptor alpha estrogen receptor beta messenger RNA octylphenol vitellogenin endocrine disruptor estrogen receptor alpha octylphenol phenol derivative vitellogenin water pollutant endocrine disruptor phenol derivative blood cichlid concentration (composition) cytoplasm dose-response relationship endocrine disruptor estrogenic compound experimental design gene expression laboratory method phenol protein adult animal experiment animal tissue article basophil Cichlasoma dimerus concentration (parameters) controlled study down regulation environmental exposure eosinophil estrogen receptor alpha gene estrogen receptor beta2 gene female gene expression regulation gene function genetic transcription genotype environment interaction histopathology male nonhuman Perciformes priority journal receptor gene risk management transactivation upregulation vitellogenesis vitellogenin gene water analysis Article basophilia Cichlasoma dimerus concentration response cytoplasm gene expression liver cell mucus nucleotide sequence Perciformes plasma sperm animal cichlid drug effects genetics liver metabolism testis toxicity water pollutant toxicity Cichlasoma Cichlidae Perciformes Animals Cichlids Endocrine Disruptors Estrogen Receptor alpha Female Gene Expression Regulation Hepatocytes Liver Male Phenols Testis Vitellogenins Water Pollutants, Chemical Genovese, Griselda Regueira, Mariana Da Cuña, Rodrigo Hernán Lo Nostro, Fabiana Laura Nonmonotonic response of vitellogenin and estrogen receptor α gene expression after octylphenol exposure of Cichlasoma dimerus (Perciformes, Cichlidae)
topic_facet	Cichlid fish Estrogen receptors Nonmonotonic dose-response Octylphenol Vitellogenin gene expression estrogen receptor alpha octylphenol phenol derivative unclassified drug vitellogenin adenosine triphosphatase (potassium sodium) alkylphenol estrogen receptor alpha estrogen receptor beta messenger RNA octylphenol vitellogenin endocrine disruptor estrogen receptor alpha octylphenol phenol derivative vitellogenin water pollutant endocrine disruptor phenol derivative blood cichlid concentration (composition) cytoplasm dose-response relationship endocrine disruptor estrogenic compound experimental design gene expression laboratory method phenol protein adult animal experiment animal tissue article basophil Cichlasoma dimerus concentration (parameters) controlled study down regulation environmental exposure eosinophil estrogen receptor alpha gene estrogen receptor beta2 gene female gene expression regulation gene function genetic transcription genotype environment interaction histopathology male nonhuman Perciformes priority journal receptor gene risk management transactivation upregulation vitellogenesis vitellogenin gene water analysis Article basophilia Cichlasoma dimerus concentration response cytoplasm gene expression liver cell mucus nucleotide sequence Perciformes plasma sperm animal cichlid drug effects genetics liver metabolism testis toxicity water pollutant toxicity Cichlasoma Cichlidae Perciformes Animals Cichlids Endocrine Disruptors Estrogen Receptor alpha Female Gene Expression Regulation Hepatocytes Liver Male Phenols Testis Vitellogenins Water Pollutants, Chemical
description	In oviparous vertebrates, vitellogenin (VTG) is mainly produced by the liver in response to estrogen (E 2 ) and its synthesis is traditionally coupled to estrogen receptor alpha induction. Even though VTG is a female-specific protein, chemicals that mimic natural estrogens, known as xenoestrogens, can activate its expression in males causing endocrine disruption to wildlife and humans. Alkylphenols such as nonylphenol (NP) and octylphenol (OP) are industrial additives used in the manufacture of a wide variety of plastics and detergents, and can disrupt endocrine functions in exposed animals. For more than a decade, the freshwater cichlid fish Cichlasoma dimerus has been used for ecotoxicological studies in our laboratory. We recently found an up-regulation of VTG gene expression in livers of male fish exposed to OP, from a silent state to values similar to those of E 2 -induced fish. To better understand the underlying mechanisms behind the action of xenoestrogens, the aim of this study was to analyze the dose-response relationship of C. dimerus VTG and estrogen receptors (ERs) gene expression after waterborne exposure to 0.15, 1.5, 15, and 150μg/L OP for up to 1 month (0, 1, 3, 7, 14, 21, and 28 days). At the end of the experiment, histological features of exposed fish included active hepatocytes with basophilic cytoplasm and high eosinophilic content in their vascular system due to augmented expression of VTG. In testis, high preponderance of sperm was found in fish exposed to 150μg/L OP. A classic dose-response down-regulation of the expression of Na + /K + -ATPase, a "non-gender specific gene" used for comparison, was found with increasing OP concentrations. No VTG and very low levels of ERα were detected in control male livers, but an up-regulation of both genes was found in males exposed to 0.15 or 150μg/L OP. Moreover, VTG transcripts were significant as early as day 3 or day 1 of exposure to these OP concentrations, respectively. Nearly no response was detected in 1.5 and 15μg/L OP exposed-fish. Data was curve-fitted evidencing a nonmonotonic dose-response curve. Interestingly, ERβ2 mRNA expression was augmented above baseline levels only when males were exposed to the lowest OP concentration. We speculate that genomic control of vitellogenesis is under control of multiple steroid receptors with different affinities for ligands. ERβ isoform, only up-regulated with very low concentrations of ligand, would act as a sensors of OP (or E 2 ) to induce ERα and VTG. With high OP concentrations, the expression of ERα isoform is promptly augmented, with the concomitant VTG transactivation. © 2014 Elsevier B.V.
author	Genovese, Griselda Regueira, Mariana Da Cuña, Rodrigo Hernán Lo Nostro, Fabiana Laura
author_facet	Genovese, Griselda Regueira, Mariana Da Cuña, Rodrigo Hernán Lo Nostro, Fabiana Laura
author_sort	Genovese, Griselda
title	Nonmonotonic response of vitellogenin and estrogen receptor α gene expression after octylphenol exposure of Cichlasoma dimerus (Perciformes, Cichlidae)
title_short	Nonmonotonic response of vitellogenin and estrogen receptor α gene expression after octylphenol exposure of Cichlasoma dimerus (Perciformes, Cichlidae)
title_full	Nonmonotonic response of vitellogenin and estrogen receptor α gene expression after octylphenol exposure of Cichlasoma dimerus (Perciformes, Cichlidae)
title_fullStr	Nonmonotonic response of vitellogenin and estrogen receptor α gene expression after octylphenol exposure of Cichlasoma dimerus (Perciformes, Cichlidae)
title_full_unstemmed	Nonmonotonic response of vitellogenin and estrogen receptor α gene expression after octylphenol exposure of Cichlasoma dimerus (Perciformes, Cichlidae)
title_sort	nonmonotonic response of vitellogenin and estrogen receptor α gene expression after octylphenol exposure of cichlasoma dimerus (perciformes, cichlidae)
publishDate	2014
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0166445X_v156_n_p30_Genovese http://hdl.handle.net/20.500.12110/paper_0166445X_v156_n_p30_Genovese
work_keys_str_mv	AT genovesegriselda nonmonotonicresponseofvitellogeninandestrogenreceptorageneexpressionafteroctylphenolexposureofcichlasomadimerusperciformescichlidae AT regueiramariana nonmonotonicresponseofvitellogeninandestrogenreceptorageneexpressionafteroctylphenolexposureofcichlasomadimerusperciformescichlidae AT dacunarodrigohernan nonmonotonicresponseofvitellogeninandestrogenreceptorageneexpressionafteroctylphenolexposureofcichlasomadimerusperciformescichlidae AT lonostrofabianalaura nonmonotonicresponseofvitellogeninandestrogenreceptorageneexpressionafteroctylphenolexposureofcichlasomadimerusperciformescichlidae
_version_	1768545550187102208
spelling	paper:paper_0166445X_v156_n_p30_Genovese2023-06-08T15:15:47Z Nonmonotonic response of vitellogenin and estrogen receptor α gene expression after octylphenol exposure of Cichlasoma dimerus (Perciformes, Cichlidae) Genovese, Griselda Regueira, Mariana Da Cuña, Rodrigo Hernán Lo Nostro, Fabiana Laura Cichlid fish Estrogen receptors Nonmonotonic dose-response Octylphenol Vitellogenin gene expression estrogen receptor alpha octylphenol phenol derivative unclassified drug vitellogenin adenosine triphosphatase (potassium sodium) alkylphenol estrogen receptor alpha estrogen receptor beta messenger RNA octylphenol vitellogenin endocrine disruptor estrogen receptor alpha octylphenol phenol derivative vitellogenin water pollutant endocrine disruptor phenol derivative blood cichlid concentration (composition) cytoplasm dose-response relationship endocrine disruptor estrogenic compound experimental design gene expression laboratory method phenol protein adult animal experiment animal tissue article basophil Cichlasoma dimerus concentration (parameters) controlled study down regulation environmental exposure eosinophil estrogen receptor alpha gene estrogen receptor beta2 gene female gene expression regulation gene function genetic transcription genotype environment interaction histopathology male nonhuman Perciformes priority journal receptor gene risk management transactivation upregulation vitellogenesis vitellogenin gene water analysis Article basophilia Cichlasoma dimerus concentration response cytoplasm gene expression liver cell mucus nucleotide sequence Perciformes plasma sperm animal cichlid drug effects genetics liver metabolism testis toxicity water pollutant toxicity Cichlasoma Cichlidae Perciformes Animals Cichlids Endocrine Disruptors Estrogen Receptor alpha Female Gene Expression Regulation Hepatocytes Liver Male Phenols Testis Vitellogenins Water Pollutants, Chemical In oviparous vertebrates, vitellogenin (VTG) is mainly produced by the liver in response to estrogen (E 2 ) and its synthesis is traditionally coupled to estrogen receptor alpha induction. Even though VTG is a female-specific protein, chemicals that mimic natural estrogens, known as xenoestrogens, can activate its expression in males causing endocrine disruption to wildlife and humans. Alkylphenols such as nonylphenol (NP) and octylphenol (OP) are industrial additives used in the manufacture of a wide variety of plastics and detergents, and can disrupt endocrine functions in exposed animals. For more than a decade, the freshwater cichlid fish Cichlasoma dimerus has been used for ecotoxicological studies in our laboratory. We recently found an up-regulation of VTG gene expression in livers of male fish exposed to OP, from a silent state to values similar to those of E 2 -induced fish. To better understand the underlying mechanisms behind the action of xenoestrogens, the aim of this study was to analyze the dose-response relationship of C. dimerus VTG and estrogen receptors (ERs) gene expression after waterborne exposure to 0.15, 1.5, 15, and 150μg/L OP for up to 1 month (0, 1, 3, 7, 14, 21, and 28 days). At the end of the experiment, histological features of exposed fish included active hepatocytes with basophilic cytoplasm and high eosinophilic content in their vascular system due to augmented expression of VTG. In testis, high preponderance of sperm was found in fish exposed to 150μg/L OP. A classic dose-response down-regulation of the expression of Na + /K + -ATPase, a "non-gender specific gene" used for comparison, was found with increasing OP concentrations. No VTG and very low levels of ERα were detected in control male livers, but an up-regulation of both genes was found in males exposed to 0.15 or 150μg/L OP. Moreover, VTG transcripts were significant as early as day 3 or day 1 of exposure to these OP concentrations, respectively. Nearly no response was detected in 1.5 and 15μg/L OP exposed-fish. Data was curve-fitted evidencing a nonmonotonic dose-response curve. Interestingly, ERβ2 mRNA expression was augmented above baseline levels only when males were exposed to the lowest OP concentration. We speculate that genomic control of vitellogenesis is under control of multiple steroid receptors with different affinities for ligands. ERβ isoform, only up-regulated with very low concentrations of ligand, would act as a sensors of OP (or E 2 ) to induce ERα and VTG. With high OP concentrations, the expression of ERα isoform is promptly augmented, with the concomitant VTG transactivation. © 2014 Elsevier B.V. Fil:Genovese, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Regueira, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Da Cuña, R.H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lo Nostro, F.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2014 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0166445X_v156_n_p30_Genovese http://hdl.handle.net/20.500.12110/paper_0166445X_v156_n_p30_Genovese

Nonmonotonic response of vitellogenin and estrogen receptor α gene expression after octylphenol exposure of Cichlasoma dimerus (Perciformes, Cichlidae)

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