Time-course recovery of estrogen-responsive genes of a cichlid fish exposed to waterborne octylphenol
The aim of this study was to describe the time-course of estrogen-induced gene expression, corresponding plasma protein detection and histological alterations after cessation of octylphenol (OP) exposure of Cichlasoma dimerus, to test differential responses of biomarkers suitable for environmental m...
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2012
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0166445X_v114-115_n_p1_Genovese http://hdl.handle.net/20.500.12110/paper_0166445X_v114-115_n_p1_Genovese |
| Aporte de: |
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paper:paper_0166445X_v114-115_n_p1_Genovese |
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| record_format |
dspace |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Cichlid Gene expression Octylphenol Recovery Vitellogenin Zona pellucida proteins adenosine triphosphatase (potassium sodium) estrogen octylphenol phenol derivative unclassified drug vitellogenin biomarker biomonitoring cichlid estrogenic compound gene expression histology phenolic compound pollution exposure protein animal experiment animal tissue article cichlid controlled study environmental exposure environmental monitoring female gene expression liver cell liver injury male nonhuman nucleotide sequence priority journal protein expression teleost upregulation water pollution zona pellucida Amino Acid Sequence Animals Cichlids Egg Proteins Endocrine Disruptors Estradiol Estrogens Female Gene Expression Regulation Male Membrane Glycoproteins Molecular Sequence Data Phenols Phylogeny Protein Isoforms Protein Subunits Receptors, Cell Surface RNA, Messenger Sodium-Potassium-Exchanging ATPase Water Pollutants, Chemical Cichlasoma Cichlidae Teleostei |
| spellingShingle |
Cichlid Gene expression Octylphenol Recovery Vitellogenin Zona pellucida proteins adenosine triphosphatase (potassium sodium) estrogen octylphenol phenol derivative unclassified drug vitellogenin biomarker biomonitoring cichlid estrogenic compound gene expression histology phenolic compound pollution exposure protein animal experiment animal tissue article cichlid controlled study environmental exposure environmental monitoring female gene expression liver cell liver injury male nonhuman nucleotide sequence priority journal protein expression teleost upregulation water pollution zona pellucida Amino Acid Sequence Animals Cichlids Egg Proteins Endocrine Disruptors Estradiol Estrogens Female Gene Expression Regulation Male Membrane Glycoproteins Molecular Sequence Data Phenols Phylogeny Protein Isoforms Protein Subunits Receptors, Cell Surface RNA, Messenger Sodium-Potassium-Exchanging ATPase Water Pollutants, Chemical Cichlasoma Cichlidae Teleostei Genovese, Griselda Regueira, Mariana Piazza, Yanina Grisel Maggese, María Cristina Irene Lo Nostro, Fabiana Laura Time-course recovery of estrogen-responsive genes of a cichlid fish exposed to waterborne octylphenol |
| topic_facet |
Cichlid Gene expression Octylphenol Recovery Vitellogenin Zona pellucida proteins adenosine triphosphatase (potassium sodium) estrogen octylphenol phenol derivative unclassified drug vitellogenin biomarker biomonitoring cichlid estrogenic compound gene expression histology phenolic compound pollution exposure protein animal experiment animal tissue article cichlid controlled study environmental exposure environmental monitoring female gene expression liver cell liver injury male nonhuman nucleotide sequence priority journal protein expression teleost upregulation water pollution zona pellucida Amino Acid Sequence Animals Cichlids Egg Proteins Endocrine Disruptors Estradiol Estrogens Female Gene Expression Regulation Male Membrane Glycoproteins Molecular Sequence Data Phenols Phylogeny Protein Isoforms Protein Subunits Receptors, Cell Surface RNA, Messenger Sodium-Potassium-Exchanging ATPase Water Pollutants, Chemical Cichlasoma Cichlidae Teleostei |
| description |
The aim of this study was to describe the time-course of estrogen-induced gene expression, corresponding plasma protein detection and histological alterations after cessation of octylphenol (OP) exposure of Cichlasoma dimerus, to test differential responses of biomarkers suitable for environmental monitoring. Male fish were exposed to a nominal concentration of 150μg/L OP for 28 days, and later transferred to OP-free water aquaria for 1, 3, 7, 14, 21 or 28 days. Blood and mucus samples were obtained in order to analyze vitellogenin (VTG) and zona pellucida (ZP) proteins by Western blot; liver samples were used for gene expression and to assess tissue damage and further recovery of all the analyzed endpoints. Partial sequences of C. dimerus VTG and Na +/K +-ATPase were obtained. Comparison with VTGs of several teleosts supports that the partial sequence obtained for C. dimerus belongs to VTGAb type. ZP and VTG expression was highly up-regulated by OP. Immunoreactive (ir-) bands of 62, 52 and 50kDa for ZP and 140, 103, 75 and 64kDa for VTG, were detected after 28 days of OP exposure in plasma and mucus samples. After transfer of treated fish to clean water, ZP ir-bands in plasma disappeared rapidly (day 3), while VTG ir-bands decreased gradually; no ir-bands were detected on day 28 of recovery. Similarly, ZPB transcripts abruptly returned to background levels (day 3), earlier than for ZPC (day 7) or VTG (day 14). Liver from OP treated fish showed tissue disarrangement, eccentric and euchromatic hepatocytes nuclei and intense perinuclear basophilia. After the recovery period, these changes were still evident though less pronounced, accounting for irreversibility of tissue damage or the requirement for a longer period of depuration. The present results confirm that for biochemical and molecular biomarkers, such as induction of female proteins in male fish exposed to OP, complete recovery is achieved after adequate time of depuration (28 days). Male ZPB expression reflects a recent exposure to estrogenic contaminants, while VTG may reveal past exposures. The combination of biomarkers with different temporal responses such as C. dimerus ZP and VTG provides a more comprehensive interpretation of pollution status. © 2012 Elsevier B.V. |
| author |
Genovese, Griselda Regueira, Mariana Piazza, Yanina Grisel Maggese, María Cristina Irene Lo Nostro, Fabiana Laura |
| author_facet |
Genovese, Griselda Regueira, Mariana Piazza, Yanina Grisel Maggese, María Cristina Irene Lo Nostro, Fabiana Laura |
| author_sort |
Genovese, Griselda |
| title |
Time-course recovery of estrogen-responsive genes of a cichlid fish exposed to waterborne octylphenol |
| title_short |
Time-course recovery of estrogen-responsive genes of a cichlid fish exposed to waterborne octylphenol |
| title_full |
Time-course recovery of estrogen-responsive genes of a cichlid fish exposed to waterborne octylphenol |
| title_fullStr |
Time-course recovery of estrogen-responsive genes of a cichlid fish exposed to waterborne octylphenol |
| title_full_unstemmed |
Time-course recovery of estrogen-responsive genes of a cichlid fish exposed to waterborne octylphenol |
| title_sort |
time-course recovery of estrogen-responsive genes of a cichlid fish exposed to waterborne octylphenol |
| publishDate |
2012 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0166445X_v114-115_n_p1_Genovese http://hdl.handle.net/20.500.12110/paper_0166445X_v114-115_n_p1_Genovese |
| work_keys_str_mv |
AT genovesegriselda timecourserecoveryofestrogenresponsivegenesofacichlidfishexposedtowaterborneoctylphenol AT regueiramariana timecourserecoveryofestrogenresponsivegenesofacichlidfishexposedtowaterborneoctylphenol AT piazzayaninagrisel timecourserecoveryofestrogenresponsivegenesofacichlidfishexposedtowaterborneoctylphenol AT maggesemariacristinairene timecourserecoveryofestrogenresponsivegenesofacichlidfishexposedtowaterborneoctylphenol AT lonostrofabianalaura timecourserecoveryofestrogenresponsivegenesofacichlidfishexposedtowaterborneoctylphenol |
| _version_ |
1768544405297299456 |
| spelling |
paper:paper_0166445X_v114-115_n_p1_Genovese2023-06-08T15:15:46Z Time-course recovery of estrogen-responsive genes of a cichlid fish exposed to waterborne octylphenol Genovese, Griselda Regueira, Mariana Piazza, Yanina Grisel Maggese, María Cristina Irene Lo Nostro, Fabiana Laura Cichlid Gene expression Octylphenol Recovery Vitellogenin Zona pellucida proteins adenosine triphosphatase (potassium sodium) estrogen octylphenol phenol derivative unclassified drug vitellogenin biomarker biomonitoring cichlid estrogenic compound gene expression histology phenolic compound pollution exposure protein animal experiment animal tissue article cichlid controlled study environmental exposure environmental monitoring female gene expression liver cell liver injury male nonhuman nucleotide sequence priority journal protein expression teleost upregulation water pollution zona pellucida Amino Acid Sequence Animals Cichlids Egg Proteins Endocrine Disruptors Estradiol Estrogens Female Gene Expression Regulation Male Membrane Glycoproteins Molecular Sequence Data Phenols Phylogeny Protein Isoforms Protein Subunits Receptors, Cell Surface RNA, Messenger Sodium-Potassium-Exchanging ATPase Water Pollutants, Chemical Cichlasoma Cichlidae Teleostei The aim of this study was to describe the time-course of estrogen-induced gene expression, corresponding plasma protein detection and histological alterations after cessation of octylphenol (OP) exposure of Cichlasoma dimerus, to test differential responses of biomarkers suitable for environmental monitoring. Male fish were exposed to a nominal concentration of 150μg/L OP for 28 days, and later transferred to OP-free water aquaria for 1, 3, 7, 14, 21 or 28 days. Blood and mucus samples were obtained in order to analyze vitellogenin (VTG) and zona pellucida (ZP) proteins by Western blot; liver samples were used for gene expression and to assess tissue damage and further recovery of all the analyzed endpoints. Partial sequences of C. dimerus VTG and Na +/K +-ATPase were obtained. Comparison with VTGs of several teleosts supports that the partial sequence obtained for C. dimerus belongs to VTGAb type. ZP and VTG expression was highly up-regulated by OP. Immunoreactive (ir-) bands of 62, 52 and 50kDa for ZP and 140, 103, 75 and 64kDa for VTG, were detected after 28 days of OP exposure in plasma and mucus samples. After transfer of treated fish to clean water, ZP ir-bands in plasma disappeared rapidly (day 3), while VTG ir-bands decreased gradually; no ir-bands were detected on day 28 of recovery. Similarly, ZPB transcripts abruptly returned to background levels (day 3), earlier than for ZPC (day 7) or VTG (day 14). Liver from OP treated fish showed tissue disarrangement, eccentric and euchromatic hepatocytes nuclei and intense perinuclear basophilia. After the recovery period, these changes were still evident though less pronounced, accounting for irreversibility of tissue damage or the requirement for a longer period of depuration. The present results confirm that for biochemical and molecular biomarkers, such as induction of female proteins in male fish exposed to OP, complete recovery is achieved after adequate time of depuration (28 days). Male ZPB expression reflects a recent exposure to estrogenic contaminants, while VTG may reveal past exposures. The combination of biomarkers with different temporal responses such as C. dimerus ZP and VTG provides a more comprehensive interpretation of pollution status. © 2012 Elsevier B.V. Fil:Genovese, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Regueira, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Piazza, Y. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Maggese, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lo Nostro, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2012 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0166445X_v114-115_n_p1_Genovese http://hdl.handle.net/20.500.12110/paper_0166445X_v114-115_n_p1_Genovese |