Early expression of zona pellucida proteins under octylphenol exposure in Cichlasoma dimerus (Perciformes, Cichlidae)

An increasing number of widely used industrial and agricultural chemicals are being found to cause endocrine disruption. In fishes, xenoestrogens can induce female proteins, and in some cases, the development of testis-ova, demonstrating feminization of males. In this study we analyzed the effect of...

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Autores principales:	Genovese, Griselda, Da Cuña, Rodrigo Hernán, Maggese, María Cristina Irene, Lo Nostro, Fabiana Laura
Publicado:	2011
Materias:	17β-Estradiol Cichlid fish Gene expression Octylphenol Vitellogenin Zona pellucida proteins alkylphenol cell protein estradiol messenger RNA octylphenol unclassified drug vitellogenin zona pellucida protein B zona pellucida protein C cichlid DNA endocrine disruptor gene expression histology protein RNA animal cell animal experiment animal tissue aquatic environment article Cichlasoma dimerus controlled study ecotoxicology endocrine disease female gene sequence germ cell histopathology liver liver cell male nonhuman nucleotide sequence Perciformes priority journal protein expression reproductive toxicity RNA extraction RNA synthesis spermatozoon count testis zona pellucida Animals Argentina Blotting, Western Cichlids Egg Proteins Estradiol Female Gene Expression Regulation Immunoblotting Liver Male Phenols Vitellogenins Zona Pellucida Cichlasoma Cichlidae Pisces
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0166445X_v101_n1_p175_Genovese http://hdl.handle.net/20.500.12110/paper_0166445X_v101_n1_p175_Genovese
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	paper:paper_0166445X_v101_n1_p175_Genovese2023-06-08T15:15:45Z Early expression of zona pellucida proteins under octylphenol exposure in Cichlasoma dimerus (Perciformes, Cichlidae) Genovese, Griselda Da Cuña, Rodrigo Hernán Maggese, María Cristina Irene Lo Nostro, Fabiana Laura 17β-Estradiol Cichlid fish Gene expression Octylphenol Vitellogenin Zona pellucida proteins alkylphenol cell protein estradiol messenger RNA octylphenol unclassified drug vitellogenin zona pellucida protein B zona pellucida protein C cichlid DNA endocrine disruptor gene expression histology protein RNA animal cell animal experiment animal tissue aquatic environment article Cichlasoma dimerus controlled study ecotoxicology endocrine disease female gene expression gene sequence germ cell histopathology liver liver cell male nonhuman nucleotide sequence Perciformes priority journal protein expression reproductive toxicity RNA extraction RNA synthesis spermatozoon count testis zona pellucida Animals Argentina Blotting, Western Cichlids Egg Proteins Estradiol Female Gene Expression Regulation Immunoblotting Liver Male Phenols Vitellogenins Zona Pellucida Cichlasoma Cichlidae Perciformes Pisces An increasing number of widely used industrial and agricultural chemicals are being found to cause endocrine disruption. In fishes, xenoestrogens can induce female proteins, and in some cases, the development of testis-ova, demonstrating feminization of males. In this study we analyzed the effect of an acute exposure of adult male Cichlasoma dimerus fish to estradiol (E2) and octylphenol (OP). E2 and OP were injected at 10 and 50μg/g body weight doses, respectively. After a single OP dose, liver was processed for RNA extraction at 1, 3, 12, 24, and 72h. PCR was performed using cDNA and primers for egg coat or zona pellucida proteins (ZP). Genes encoding ZPB and ZPC isoforms were sequenced. E2-induced fish were sacrificed at 72h. Using multiple OP or E2 injections, blood and surface mucus were sampled on days 0, 3, 6, 9, and 13. On day 13 fish were sacrificed for liver and testis dissection. Histological examination of E2 and OP-treated fish livers showed cellular disarray and intense cytoplasmatic basophilia within hepatocytes, probably due to increased mRNA synthesis, as well as hypertrophied euchromatic nuclei, and conspicuous nucleoli, indicative of augmented cell activity. An abnormal amount of sperm and immature germ cells within the testis lumen were seen in treated fish, suggesting reproductive impairment. Both plasma and mucus revealed the presence of ZP (and vitellogenin) at day 3 and thereafter with E2 treatment, using Western and Dot blot techniques; OP effects were delayed in time. These results validate the analysis of mucus by Dot blot as an easy and rapid technique to address endocrine disruption caused by OP. Quantitative gene expression showed induction of liver ZPB and ZPC upon OP injection; muscle, brain, and intestine did not express any ZP. Both ZPs were induced at 1h post injection, but only ZPB expression was statistically significant. At 12h, both ZPs increased significantly, reaching the same levels of E2-challenged males after 72h. Therefore, OP mimicked the action of E2 with a prompt and strong xenoestrogenic effect, evidenced by the early response through mRNA and protein expression of ZP and the concomitant histological liver and testis alterations. © 2010 Elsevier B.V. Fil:Genovese, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Da Cuña, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Maggese, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lo Nostro, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2011 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0166445X_v101_n1_p175_Genovese http://hdl.handle.net/20.500.12110/paper_0166445X_v101_n1_p175_Genovese
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	17β-Estradiol Cichlid fish Gene expression Octylphenol Vitellogenin Zona pellucida proteins alkylphenol cell protein estradiol messenger RNA octylphenol unclassified drug vitellogenin zona pellucida protein B zona pellucida protein C cichlid DNA endocrine disruptor gene expression histology protein RNA animal cell animal experiment animal tissue aquatic environment article Cichlasoma dimerus controlled study ecotoxicology endocrine disease female gene expression gene sequence germ cell histopathology liver liver cell male nonhuman nucleotide sequence Perciformes priority journal protein expression reproductive toxicity RNA extraction RNA synthesis spermatozoon count testis zona pellucida Animals Argentina Blotting, Western Cichlids Egg Proteins Estradiol Female Gene Expression Regulation Immunoblotting Liver Male Phenols Vitellogenins Zona Pellucida Cichlasoma Cichlidae Perciformes Pisces
spellingShingle	17β-Estradiol Cichlid fish Gene expression Octylphenol Vitellogenin Zona pellucida proteins alkylphenol cell protein estradiol messenger RNA octylphenol unclassified drug vitellogenin zona pellucida protein B zona pellucida protein C cichlid DNA endocrine disruptor gene expression histology protein RNA animal cell animal experiment animal tissue aquatic environment article Cichlasoma dimerus controlled study ecotoxicology endocrine disease female gene expression gene sequence germ cell histopathology liver liver cell male nonhuman nucleotide sequence Perciformes priority journal protein expression reproductive toxicity RNA extraction RNA synthesis spermatozoon count testis zona pellucida Animals Argentina Blotting, Western Cichlids Egg Proteins Estradiol Female Gene Expression Regulation Immunoblotting Liver Male Phenols Vitellogenins Zona Pellucida Cichlasoma Cichlidae Perciformes Pisces Genovese, Griselda Da Cuña, Rodrigo Hernán Maggese, María Cristina Irene Lo Nostro, Fabiana Laura Early expression of zona pellucida proteins under octylphenol exposure in Cichlasoma dimerus (Perciformes, Cichlidae)
topic_facet	17β-Estradiol Cichlid fish Gene expression Octylphenol Vitellogenin Zona pellucida proteins alkylphenol cell protein estradiol messenger RNA octylphenol unclassified drug vitellogenin zona pellucida protein B zona pellucida protein C cichlid DNA endocrine disruptor gene expression histology protein RNA animal cell animal experiment animal tissue aquatic environment article Cichlasoma dimerus controlled study ecotoxicology endocrine disease female gene expression gene sequence germ cell histopathology liver liver cell male nonhuman nucleotide sequence Perciformes priority journal protein expression reproductive toxicity RNA extraction RNA synthesis spermatozoon count testis zona pellucida Animals Argentina Blotting, Western Cichlids Egg Proteins Estradiol Female Gene Expression Regulation Immunoblotting Liver Male Phenols Vitellogenins Zona Pellucida Cichlasoma Cichlidae Perciformes Pisces
description	An increasing number of widely used industrial and agricultural chemicals are being found to cause endocrine disruption. In fishes, xenoestrogens can induce female proteins, and in some cases, the development of testis-ova, demonstrating feminization of males. In this study we analyzed the effect of an acute exposure of adult male Cichlasoma dimerus fish to estradiol (E2) and octylphenol (OP). E2 and OP were injected at 10 and 50μg/g body weight doses, respectively. After a single OP dose, liver was processed for RNA extraction at 1, 3, 12, 24, and 72h. PCR was performed using cDNA and primers for egg coat or zona pellucida proteins (ZP). Genes encoding ZPB and ZPC isoforms were sequenced. E2-induced fish were sacrificed at 72h. Using multiple OP or E2 injections, blood and surface mucus were sampled on days 0, 3, 6, 9, and 13. On day 13 fish were sacrificed for liver and testis dissection. Histological examination of E2 and OP-treated fish livers showed cellular disarray and intense cytoplasmatic basophilia within hepatocytes, probably due to increased mRNA synthesis, as well as hypertrophied euchromatic nuclei, and conspicuous nucleoli, indicative of augmented cell activity. An abnormal amount of sperm and immature germ cells within the testis lumen were seen in treated fish, suggesting reproductive impairment. Both plasma and mucus revealed the presence of ZP (and vitellogenin) at day 3 and thereafter with E2 treatment, using Western and Dot blot techniques; OP effects were delayed in time. These results validate the analysis of mucus by Dot blot as an easy and rapid technique to address endocrine disruption caused by OP. Quantitative gene expression showed induction of liver ZPB and ZPC upon OP injection; muscle, brain, and intestine did not express any ZP. Both ZPs were induced at 1h post injection, but only ZPB expression was statistically significant. At 12h, both ZPs increased significantly, reaching the same levels of E2-challenged males after 72h. Therefore, OP mimicked the action of E2 with a prompt and strong xenoestrogenic effect, evidenced by the early response through mRNA and protein expression of ZP and the concomitant histological liver and testis alterations. © 2010 Elsevier B.V.
author	Genovese, Griselda Da Cuña, Rodrigo Hernán Maggese, María Cristina Irene Lo Nostro, Fabiana Laura
author_facet	Genovese, Griselda Da Cuña, Rodrigo Hernán Maggese, María Cristina Irene Lo Nostro, Fabiana Laura
author_sort	Genovese, Griselda
title	Early expression of zona pellucida proteins under octylphenol exposure in Cichlasoma dimerus (Perciformes, Cichlidae)
title_short	Early expression of zona pellucida proteins under octylphenol exposure in Cichlasoma dimerus (Perciformes, Cichlidae)
title_full	Early expression of zona pellucida proteins under octylphenol exposure in Cichlasoma dimerus (Perciformes, Cichlidae)
title_fullStr	Early expression of zona pellucida proteins under octylphenol exposure in Cichlasoma dimerus (Perciformes, Cichlidae)
title_full_unstemmed	Early expression of zona pellucida proteins under octylphenol exposure in Cichlasoma dimerus (Perciformes, Cichlidae)
title_sort	early expression of zona pellucida proteins under octylphenol exposure in cichlasoma dimerus (perciformes, cichlidae)
publishDate	2011
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0166445X_v101_n1_p175_Genovese http://hdl.handle.net/20.500.12110/paper_0166445X_v101_n1_p175_Genovese
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Early expression of zona pellucida proteins under octylphenol exposure in Cichlasoma dimerus (Perciformes, Cichlidae)

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