Antiviral effects of Melia azedarach L. leaves extracts on Sindbis virus-infected cells

Partially purified extracts from leaves of Melia azedarach L. (MA) exert a broad range of antiviral effects on DNA and RNA viruses. The effect of MA on different stages of Sindbis virus replicative cycle in BHK cells was investigated. Under one-step growth conditions MA afforded a greater than 90% i...

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Autores principales: Wachsman, Mónica Beatriz, Damonte, Elsa Beatriz, Coto, Celia Esther
Publicado: 1987
Materias:
Melia azedarach L
Sindbis virus
antivirus agent
plant extract
cell culture
drug analysis
drug mechanism
electrophoresis
higher plant
in vitro study
melia azedarach
nonhuman
protein synthesis
rna synthesis
sindbis virus
therapy
virus cell interaction
virus infection
Antiviral Agents
Cell Adhesion
Cell Line
Plant Extracts
RNA, Viral
Sindbis Virus
Support, Non-U.S. Gov't
Viral Proteins
Virus Replication
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01663542_v8_n1_p1_Wachsman
http://hdl.handle.net/20.500.12110/paper_01663542_v8_n1_p1_Wachsman
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_01663542_v8_n1_p1_Wachsman
record_format dspace
spelling paper:paper_01663542_v8_n1_p1_Wachsman2023-06-08T15:15:41Z Antiviral effects of Melia azedarach L. leaves extracts on Sindbis virus-infected cells Wachsman, Mónica Beatriz Damonte, Elsa Beatriz Coto, Celia Esther Melia azedarach L Sindbis virus antivirus agent plant extract cell culture drug analysis drug mechanism electrophoresis higher plant in vitro study melia azedarach nonhuman protein synthesis rna synthesis sindbis virus therapy virus cell interaction virus infection Antiviral Agents Cell Adhesion Cell Line Plant Extracts RNA, Viral Sindbis Virus Support, Non-U.S. Gov't Viral Proteins Virus Replication Partially purified extracts from leaves of Melia azedarach L. (MA) exert a broad range of antiviral effects on DNA and RNA viruses. The effect of MA on different stages of Sindbis virus replicative cycle in BHK cells was investigated. Under one-step growth conditions MA afforded a greater than 90% inhibition in virus yield if added to the cell cultures 2 h before or after infection, and when added 4 h after infection MA still caused a greater than 80% inhibition. Analysis of early events following Sindbis virus infection showed that MA did not affect viral adsorption to or penetration in BHK cell. In contrast, viral RNA and protein synthesis was almost totally inhibited in cells pretreated with MA 2 h before infection, while cellular macromolecular synthesis was similar in MA-treated and untreated cell cultures. © 1987. Fil:Wachsman, M.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Damonte, E.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Coto, C.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1987 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01663542_v8_n1_p1_Wachsman http://hdl.handle.net/20.500.12110/paper_01663542_v8_n1_p1_Wachsman
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Melia azedarach L
Sindbis virus
antivirus agent
plant extract
cell culture
drug analysis
drug mechanism
electrophoresis
higher plant
in vitro study
melia azedarach
nonhuman
protein synthesis
rna synthesis
sindbis virus
therapy
virus cell interaction
virus infection
Antiviral Agents
Cell Adhesion
Cell Line
Plant Extracts
RNA, Viral
Sindbis Virus
Support, Non-U.S. Gov't
Viral Proteins
Virus Replication
spellingShingle Melia azedarach L
Sindbis virus
antivirus agent
plant extract
cell culture
drug analysis
drug mechanism
electrophoresis
higher plant
in vitro study
melia azedarach
nonhuman
protein synthesis
rna synthesis
sindbis virus
therapy
virus cell interaction
virus infection
Antiviral Agents
Cell Adhesion
Cell Line
Plant Extracts
RNA, Viral
Sindbis Virus
Support, Non-U.S. Gov't
Viral Proteins
Virus Replication
Wachsman, Mónica Beatriz
Damonte, Elsa Beatriz
Coto, Celia Esther
Antiviral effects of Melia azedarach L. leaves extracts on Sindbis virus-infected cells
topic_facet Melia azedarach L
Sindbis virus
antivirus agent
plant extract
cell culture
drug analysis
drug mechanism
electrophoresis
higher plant
in vitro study
melia azedarach
nonhuman
protein synthesis
rna synthesis
sindbis virus
therapy
virus cell interaction
virus infection
Antiviral Agents
Cell Adhesion
Cell Line
Plant Extracts
RNA, Viral
Sindbis Virus
Support, Non-U.S. Gov't
Viral Proteins
Virus Replication
description Partially purified extracts from leaves of Melia azedarach L. (MA) exert a broad range of antiviral effects on DNA and RNA viruses. The effect of MA on different stages of Sindbis virus replicative cycle in BHK cells was investigated. Under one-step growth conditions MA afforded a greater than 90% inhibition in virus yield if added to the cell cultures 2 h before or after infection, and when added 4 h after infection MA still caused a greater than 80% inhibition. Analysis of early events following Sindbis virus infection showed that MA did not affect viral adsorption to or penetration in BHK cell. In contrast, viral RNA and protein synthesis was almost totally inhibited in cells pretreated with MA 2 h before infection, while cellular macromolecular synthesis was similar in MA-treated and untreated cell cultures. © 1987.
author Wachsman, Mónica Beatriz
Damonte, Elsa Beatriz
Coto, Celia Esther
author_facet Wachsman, Mónica Beatriz
Damonte, Elsa Beatriz
Coto, Celia Esther
author_sort Wachsman, Mónica Beatriz
title Antiviral effects of Melia azedarach L. leaves extracts on Sindbis virus-infected cells
title_short Antiviral effects of Melia azedarach L. leaves extracts on Sindbis virus-infected cells
title_full Antiviral effects of Melia azedarach L. leaves extracts on Sindbis virus-infected cells
title_fullStr Antiviral effects of Melia azedarach L. leaves extracts on Sindbis virus-infected cells
title_full_unstemmed Antiviral effects of Melia azedarach L. leaves extracts on Sindbis virus-infected cells
title_sort antiviral effects of melia azedarach l. leaves extracts on sindbis virus-infected cells
publishDate 1987
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01663542_v8_n1_p1_Wachsman
http://hdl.handle.net/20.500.12110/paper_01663542_v8_n1_p1_Wachsman
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AT damonteelsabeatriz antiviraleffectsofmeliaazedarachlleavesextractsonsindbisvirusinfectedcells
AT cotoceliaesther antiviraleffectsofmeliaazedarachlleavesextractsonsindbisvirusinfectedcells
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