Inhibition of arenavirus infection by thiuram and aromatic disulfides

A selected group of aromatic disulfides, thiuram disulfides and thiosulfones, provided by the National Cancer Institute, were evaluated in vitro for their inhibitory activity against Junin virus (JUNV), the causative agent of Argentine hemorrhagic fever. The aromatic disulfides NSC4492 and NSC71033...

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Autores principales:	Sepúlveda, Claudia Soledad, García, Cybele Carina, Damonte, Elsa Beatriz
Publicado:	2010
Materias:	Antiviral Arenavirus Disulfides Junin virus Virucidal aromatic disulfide disulfide disulfiram fatty acid binding protein nsc 112801 nsc 1339 nsc 14560 nsc 211 nsc 238936 nsc 255089 nsc 27320 nsc 27360 nsc 314654 nsc 317926 nsc 327174 nsc 342015 nsc 35825 nsc 38069 nsc 402561 nsc 4492 nsc 5239 nsc 58950 nsc 71033 nsc 76302 nsc 83217 nsc 93057 nsc 93058 ribavirin unclassified drug virus antigen animal cell antigen expression antiviral activity arenavirus infection Argentine hemorrhagic fever article cell strain BHK controlled study drug cytotoxicity drug structure human human cell IC 50 immunofluorescence nonhuman priority journal RING finger motif Vero cell virucidal activity virus attachment virus entry virus infectivity virus inhibition Animals Antiviral Agents Cell Line Cercopithecus aethiops Cricetinae Humans Inhibitory Concentration 50 Microbial Sensitivity Tests Microbial Viability Thiram Virus Replication
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01663542_v87_n3_p329_Sepulveda http://hdl.handle.net/20.500.12110/paper_01663542_v87_n3_p329_Sepulveda
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_01663542_v87_n3_p329_Sepulveda
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spelling	paper:paper_01663542_v87_n3_p329_Sepulveda2023-06-08T15:15:41Z Inhibition of arenavirus infection by thiuram and aromatic disulfides Sepúlveda, Claudia Soledad García, Cybele Carina Damonte, Elsa Beatriz Antiviral Arenavirus Disulfides Junin virus Virucidal aromatic disulfide disulfide disulfiram fatty acid binding protein nsc 112801 nsc 1339 nsc 14560 nsc 211 nsc 238936 nsc 255089 nsc 27320 nsc 27360 nsc 314654 nsc 317926 nsc 327174 nsc 342015 nsc 35825 nsc 38069 nsc 402561 nsc 4492 nsc 5239 nsc 58950 nsc 71033 nsc 76302 nsc 83217 nsc 93057 nsc 93058 ribavirin unclassified drug virus antigen animal cell antigen expression antiviral activity Arenavirus arenavirus infection Argentine hemorrhagic fever article cell strain BHK controlled study drug cytotoxicity drug structure human human cell IC 50 immunofluorescence Junin virus nonhuman priority journal RING finger motif Vero cell virucidal activity virus attachment virus entry virus infectivity virus inhibition Animals Antiviral Agents Cell Line Cercopithecus aethiops Cricetinae Disulfides Humans Inhibitory Concentration 50 Junin virus Microbial Sensitivity Tests Microbial Viability Thiram Virus Replication Arenavirus Cricetinae Junin virus A selected group of aromatic disulfides, thiuram disulfides and thiosulfones, provided by the National Cancer Institute, were evaluated in vitro for their inhibitory activity against Junin virus (JUNV), the causative agent of Argentine hemorrhagic fever. The aromatic disulfides NSC4492 and NSC71033 and the thiuram disulfide NSC14560 were, respectively, the more potent virucidal and antiviral agents against JUNV, with inactivating concentration 50% (IC50) values of 0.2-0.5μM for virucidal compounds and antiviral effective concentration 50% (EC50) of 8.5μM for NSC14560. Both types of compounds exhibited inhibitory activity against three arenaviruses. Additionally, a comparable efficacy in the antiviral action of NSC14560 was observed in monkey, hamster or human cells with selectivity indices in the range 55.9-85.7. Time of addition experiments showed that the main antiviral activity of NSC14560 was situated before 5h of infection, but a significant inhibition was still observed when the compound was added up 9h p.i. This compound did not induce a refractory state to infection by cell pretreatment. Nor did it prevent viral entry, but the cytoplasmic and membrane expression of the main viral proteins was inhibited. The possible involvement of the RING finger motif of arenavirus Z protein as target for the thiuram disulfide is discussed. © 2010 Elsevier B.V. Fil:Sepúlveda, C.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:García, C.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Damonte, E.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2010 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01663542_v87_n3_p329_Sepulveda http://hdl.handle.net/20.500.12110/paper_01663542_v87_n3_p329_Sepulveda
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Antiviral Arenavirus Disulfides Junin virus Virucidal aromatic disulfide disulfide disulfiram fatty acid binding protein nsc 112801 nsc 1339 nsc 14560 nsc 211 nsc 238936 nsc 255089 nsc 27320 nsc 27360 nsc 314654 nsc 317926 nsc 327174 nsc 342015 nsc 35825 nsc 38069 nsc 402561 nsc 4492 nsc 5239 nsc 58950 nsc 71033 nsc 76302 nsc 83217 nsc 93057 nsc 93058 ribavirin unclassified drug virus antigen animal cell antigen expression antiviral activity Arenavirus arenavirus infection Argentine hemorrhagic fever article cell strain BHK controlled study drug cytotoxicity drug structure human human cell IC 50 immunofluorescence Junin virus nonhuman priority journal RING finger motif Vero cell virucidal activity virus attachment virus entry virus infectivity virus inhibition Animals Antiviral Agents Cell Line Cercopithecus aethiops Cricetinae Disulfides Humans Inhibitory Concentration 50 Junin virus Microbial Sensitivity Tests Microbial Viability Thiram Virus Replication Arenavirus Cricetinae Junin virus
spellingShingle	Antiviral Arenavirus Disulfides Junin virus Virucidal aromatic disulfide disulfide disulfiram fatty acid binding protein nsc 112801 nsc 1339 nsc 14560 nsc 211 nsc 238936 nsc 255089 nsc 27320 nsc 27360 nsc 314654 nsc 317926 nsc 327174 nsc 342015 nsc 35825 nsc 38069 nsc 402561 nsc 4492 nsc 5239 nsc 58950 nsc 71033 nsc 76302 nsc 83217 nsc 93057 nsc 93058 ribavirin unclassified drug virus antigen animal cell antigen expression antiviral activity Arenavirus arenavirus infection Argentine hemorrhagic fever article cell strain BHK controlled study drug cytotoxicity drug structure human human cell IC 50 immunofluorescence Junin virus nonhuman priority journal RING finger motif Vero cell virucidal activity virus attachment virus entry virus infectivity virus inhibition Animals Antiviral Agents Cell Line Cercopithecus aethiops Cricetinae Disulfides Humans Inhibitory Concentration 50 Junin virus Microbial Sensitivity Tests Microbial Viability Thiram Virus Replication Arenavirus Cricetinae Junin virus Sepúlveda, Claudia Soledad García, Cybele Carina Damonte, Elsa Beatriz Inhibition of arenavirus infection by thiuram and aromatic disulfides
topic_facet	Antiviral Arenavirus Disulfides Junin virus Virucidal aromatic disulfide disulfide disulfiram fatty acid binding protein nsc 112801 nsc 1339 nsc 14560 nsc 211 nsc 238936 nsc 255089 nsc 27320 nsc 27360 nsc 314654 nsc 317926 nsc 327174 nsc 342015 nsc 35825 nsc 38069 nsc 402561 nsc 4492 nsc 5239 nsc 58950 nsc 71033 nsc 76302 nsc 83217 nsc 93057 nsc 93058 ribavirin unclassified drug virus antigen animal cell antigen expression antiviral activity Arenavirus arenavirus infection Argentine hemorrhagic fever article cell strain BHK controlled study drug cytotoxicity drug structure human human cell IC 50 immunofluorescence Junin virus nonhuman priority journal RING finger motif Vero cell virucidal activity virus attachment virus entry virus infectivity virus inhibition Animals Antiviral Agents Cell Line Cercopithecus aethiops Cricetinae Disulfides Humans Inhibitory Concentration 50 Junin virus Microbial Sensitivity Tests Microbial Viability Thiram Virus Replication Arenavirus Cricetinae Junin virus
description	A selected group of aromatic disulfides, thiuram disulfides and thiosulfones, provided by the National Cancer Institute, were evaluated in vitro for their inhibitory activity against Junin virus (JUNV), the causative agent of Argentine hemorrhagic fever. The aromatic disulfides NSC4492 and NSC71033 and the thiuram disulfide NSC14560 were, respectively, the more potent virucidal and antiviral agents against JUNV, with inactivating concentration 50% (IC50) values of 0.2-0.5μM for virucidal compounds and antiviral effective concentration 50% (EC50) of 8.5μM for NSC14560. Both types of compounds exhibited inhibitory activity against three arenaviruses. Additionally, a comparable efficacy in the antiviral action of NSC14560 was observed in monkey, hamster or human cells with selectivity indices in the range 55.9-85.7. Time of addition experiments showed that the main antiviral activity of NSC14560 was situated before 5h of infection, but a significant inhibition was still observed when the compound was added up 9h p.i. This compound did not induce a refractory state to infection by cell pretreatment. Nor did it prevent viral entry, but the cytoplasmic and membrane expression of the main viral proteins was inhibited. The possible involvement of the RING finger motif of arenavirus Z protein as target for the thiuram disulfide is discussed. © 2010 Elsevier B.V.
author	Sepúlveda, Claudia Soledad García, Cybele Carina Damonte, Elsa Beatriz
author_facet	Sepúlveda, Claudia Soledad García, Cybele Carina Damonte, Elsa Beatriz
author_sort	Sepúlveda, Claudia Soledad
title	Inhibition of arenavirus infection by thiuram and aromatic disulfides
title_short	Inhibition of arenavirus infection by thiuram and aromatic disulfides
title_full	Inhibition of arenavirus infection by thiuram and aromatic disulfides
title_fullStr	Inhibition of arenavirus infection by thiuram and aromatic disulfides
title_full_unstemmed	Inhibition of arenavirus infection by thiuram and aromatic disulfides
title_sort	inhibition of arenavirus infection by thiuram and aromatic disulfides
publishDate	2010
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01663542_v87_n3_p329_Sepulveda http://hdl.handle.net/20.500.12110/paper_01663542_v87_n3_p329_Sepulveda
work_keys_str_mv	AT sepulvedaclaudiasoledad inhibitionofarenavirusinfectionbythiuramandaromaticdisulfides AT garciacybelecarina inhibitionofarenavirusinfectionbythiuramandaromaticdisulfides AT damonteelsabeatriz inhibitionofarenavirusinfectionbythiuramandaromaticdisulfides
_version_	1768542547714506752

Inhibition of arenavirus infection by thiuram and aromatic disulfides

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