Mode of inactivation of arenaviruses by disulfide-based compounds

Several disulfide-based compounds, including intermolecular aromatic disulfides of the type Ph-S-S-Ph and dithianes with the sulfur atoms tethered in a ring structure, have shown effective inhibitory activity against the arenaviruses Junin (JUNV), agent of Argentine hemorrhagic fever, and Tacaribe (...

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Publicado:	2002
Materias:	Antiviral Arenaviruses Disulfides Junin virus Tacaribe virus Virus inactivation antivirus agent disulfide n 2 100 6 nsc 20625 nsc 4493 nsc 624125 nsc 624151 nsc 624152 nsc 677459 unclassified drug virus protein animal cell Arenavirus article binding kinetics concentration response controlled study IC 50 nonhuman priority journal protein synthesis time Vero cell virion virus inactivation virus particle virus replication Animals Antiviral Agents Arenaviruses, New World Cercopithecus aethiops Vero Cells Viral Proteins Virion Virus Replication Argentina (fish)
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01663542_v55_n3_p437_Garcia http://hdl.handle.net/20.500.12110/paper_01663542_v55_n3_p437_Garcia
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_01663542_v55_n3_p437_Garcia
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spelling	paper:paper_01663542_v55_n3_p437_Garcia2023-06-08T15:15:39Z Mode of inactivation of arenaviruses by disulfide-based compounds Antiviral Arenaviruses Disulfides Junin virus Tacaribe virus Virus inactivation antivirus agent disulfide n 2 100 6 nsc 20625 nsc 4493 nsc 624125 nsc 624151 nsc 624152 nsc 677459 unclassified drug virus protein animal cell Arenavirus article binding kinetics concentration response controlled study IC 50 Junin virus nonhuman priority journal protein synthesis time Vero cell virion virus inactivation virus particle virus replication Animals Antiviral Agents Arenaviruses, New World Cercopithecus aethiops Disulfides Junin virus Vero Cells Viral Proteins Virion Virus Replication Argentina (fish) Junin virus Tacaribe virus Several disulfide-based compounds, including intermolecular aromatic disulfides of the type Ph-S-S-Ph and dithianes with the sulfur atoms tethered in a ring structure, have shown effective inhibitory activity against the arenaviruses Junin (JUNV), agent of Argentine hemorrhagic fever, and Tacaribe (TCRV). These compounds showed a strong virucidal effect with inactivating concentration 50% (IC50) values in the range 0.6-5.0 μM, and also were effective to reduce virus yields from infected cells. The mode of inactivating action of two active compounds, the aromatic bis disulfide NSC20625 and the dithiane NSC624152, was further studied. Both compounds were able to inactivate arenaviruses after a few minutes of direct contact with virions, in a concentration- and time-dependent manner. The ability of drug-treated virus to perform several steps of the replication cycle was analyzed. The killed virus particles were found to bind and enter to Vero cells with the same efficacy as infectious native virions, but the ability of inactivated virions to synthesize viral proteins in Vero cells was abolished. Thus, treatment of JUNV and TCRV with these compounds destroyed virion infectivity, generating particles which entered the host cell but were unable to complete the viral biosynthetic processes. © 2002 Elsevier Science B.V. All rights reserved. 2002 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01663542_v55_n3_p437_Garcia http://hdl.handle.net/20.500.12110/paper_01663542_v55_n3_p437_Garcia
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Antiviral Arenaviruses Disulfides Junin virus Tacaribe virus Virus inactivation antivirus agent disulfide n 2 100 6 nsc 20625 nsc 4493 nsc 624125 nsc 624151 nsc 624152 nsc 677459 unclassified drug virus protein animal cell Arenavirus article binding kinetics concentration response controlled study IC 50 Junin virus nonhuman priority journal protein synthesis time Vero cell virion virus inactivation virus particle virus replication Animals Antiviral Agents Arenaviruses, New World Cercopithecus aethiops Disulfides Junin virus Vero Cells Viral Proteins Virion Virus Replication Argentina (fish) Junin virus Tacaribe virus
spellingShingle	Antiviral Arenaviruses Disulfides Junin virus Tacaribe virus Virus inactivation antivirus agent disulfide n 2 100 6 nsc 20625 nsc 4493 nsc 624125 nsc 624151 nsc 624152 nsc 677459 unclassified drug virus protein animal cell Arenavirus article binding kinetics concentration response controlled study IC 50 Junin virus nonhuman priority journal protein synthesis time Vero cell virion virus inactivation virus particle virus replication Animals Antiviral Agents Arenaviruses, New World Cercopithecus aethiops Disulfides Junin virus Vero Cells Viral Proteins Virion Virus Replication Argentina (fish) Junin virus Tacaribe virus Mode of inactivation of arenaviruses by disulfide-based compounds
topic_facet	Antiviral Arenaviruses Disulfides Junin virus Tacaribe virus Virus inactivation antivirus agent disulfide n 2 100 6 nsc 20625 nsc 4493 nsc 624125 nsc 624151 nsc 624152 nsc 677459 unclassified drug virus protein animal cell Arenavirus article binding kinetics concentration response controlled study IC 50 Junin virus nonhuman priority journal protein synthesis time Vero cell virion virus inactivation virus particle virus replication Animals Antiviral Agents Arenaviruses, New World Cercopithecus aethiops Disulfides Junin virus Vero Cells Viral Proteins Virion Virus Replication Argentina (fish) Junin virus Tacaribe virus
description	Several disulfide-based compounds, including intermolecular aromatic disulfides of the type Ph-S-S-Ph and dithianes with the sulfur atoms tethered in a ring structure, have shown effective inhibitory activity against the arenaviruses Junin (JUNV), agent of Argentine hemorrhagic fever, and Tacaribe (TCRV). These compounds showed a strong virucidal effect with inactivating concentration 50% (IC50) values in the range 0.6-5.0 μM, and also were effective to reduce virus yields from infected cells. The mode of inactivating action of two active compounds, the aromatic bis disulfide NSC20625 and the dithiane NSC624152, was further studied. Both compounds were able to inactivate arenaviruses after a few minutes of direct contact with virions, in a concentration- and time-dependent manner. The ability of drug-treated virus to perform several steps of the replication cycle was analyzed. The killed virus particles were found to bind and enter to Vero cells with the same efficacy as infectious native virions, but the ability of inactivated virions to synthesize viral proteins in Vero cells was abolished. Thus, treatment of JUNV and TCRV with these compounds destroyed virion infectivity, generating particles which entered the host cell but were unable to complete the viral biosynthetic processes. © 2002 Elsevier Science B.V. All rights reserved.
title	Mode of inactivation of arenaviruses by disulfide-based compounds
title_short	Mode of inactivation of arenaviruses by disulfide-based compounds
title_full	Mode of inactivation of arenaviruses by disulfide-based compounds
title_fullStr	Mode of inactivation of arenaviruses by disulfide-based compounds
title_full_unstemmed	Mode of inactivation of arenaviruses by disulfide-based compounds
title_sort	mode of inactivation of arenaviruses by disulfide-based compounds
publishDate	2002
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01663542_v55_n3_p437_Garcia http://hdl.handle.net/20.500.12110/paper_01663542_v55_n3_p437_Garcia
_version_	1768544590194802688

Mode of inactivation of arenaviruses by disulfide-based compounds

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