Coupling of foot-and-mouth disease virus to sheep red blood cells using tannic acid for immunological assays

A technique for coupling foot-and-mouth disease virus (FMDV) to tanned sheep red blood cells (SRBC) is reported. Different parameters influencing the procedure were studied. Subtypes C2, C3, O1 and A24 were used as antigens, and guinea pig hyperimmune sera obtained were tested for specific antibody...

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Autores principales:	Pujol, Carlos Alberto, Massouh, Ernesto Jorge
Publicado:	1988
Materias:	virus antibody virus antigen cell culture foot and mouth disease virus hemagglutination nonhuman priority journal serum Animal Antigens, Viral Aphthovirus Erythrocytes Hemagglutination Inhibition Tests Hemagglutination Tests Hemolytic Plaque Technique Immunologic Techniques In Vitro Microbiological Techniques Sheep Support, Non-U.S. Gov't Tannic Acid
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01660934_v20_n4_p275_Pujol http://hdl.handle.net/20.500.12110/paper_01660934_v20_n4_p275_Pujol
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_01660934_v20_n4_p275_Pujol
record_format	dspace
spelling	paper:paper_01660934_v20_n4_p275_Pujol2023-06-08T15:15:06Z Coupling of foot-and-mouth disease virus to sheep red blood cells using tannic acid for immunological assays Pujol, Carlos Alberto Massouh, Ernesto Jorge virus antibody virus antigen cell culture foot and mouth disease virus hemagglutination nonhuman priority journal serum Animal Antigens, Viral Aphthovirus Erythrocytes Hemagglutination Inhibition Tests Hemagglutination Tests Hemolytic Plaque Technique Immunologic Techniques In Vitro Microbiological Techniques Sheep Support, Non-U.S. Gov't Tannic Acid A technique for coupling foot-and-mouth disease virus (FMDV) to tanned sheep red blood cells (SRBC) is reported. Different parameters influencing the procedure were studied. Subtypes C2, C3, O1 and A24 were used as antigens, and guinea pig hyperimmune sera obtained were tested for specific antibody in passive hemagglutination (PH), passive hemagglutination inhibition (PHI) and passive immune hemolysis (PIL) assays. Fresh and SRBC stored in Alsever's solution showed similar behavior when used as indicator cells. Optimal sensitization of erythrocytes was achieved using tannic acid 1:20000 and 20 μg of purified virus/ml at pH 7.6. Specificity of the reaction was confirmed by PH and PHI in homologous and heterologous systems. The coupled antigen-antibody complex was sensitive to complement mediated lysis in a PIL test. Fil:Pujol, C.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Massouh, E.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1988 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01660934_v20_n4_p275_Pujol http://hdl.handle.net/20.500.12110/paper_01660934_v20_n4_p275_Pujol
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	virus antibody virus antigen cell culture foot and mouth disease virus hemagglutination nonhuman priority journal serum Animal Antigens, Viral Aphthovirus Erythrocytes Hemagglutination Inhibition Tests Hemagglutination Tests Hemolytic Plaque Technique Immunologic Techniques In Vitro Microbiological Techniques Sheep Support, Non-U.S. Gov't Tannic Acid
spellingShingle	virus antibody virus antigen cell culture foot and mouth disease virus hemagglutination nonhuman priority journal serum Animal Antigens, Viral Aphthovirus Erythrocytes Hemagglutination Inhibition Tests Hemagglutination Tests Hemolytic Plaque Technique Immunologic Techniques In Vitro Microbiological Techniques Sheep Support, Non-U.S. Gov't Tannic Acid Pujol, Carlos Alberto Massouh, Ernesto Jorge Coupling of foot-and-mouth disease virus to sheep red blood cells using tannic acid for immunological assays
topic_facet	virus antibody virus antigen cell culture foot and mouth disease virus hemagglutination nonhuman priority journal serum Animal Antigens, Viral Aphthovirus Erythrocytes Hemagglutination Inhibition Tests Hemagglutination Tests Hemolytic Plaque Technique Immunologic Techniques In Vitro Microbiological Techniques Sheep Support, Non-U.S. Gov't Tannic Acid
description	A technique for coupling foot-and-mouth disease virus (FMDV) to tanned sheep red blood cells (SRBC) is reported. Different parameters influencing the procedure were studied. Subtypes C2, C3, O1 and A24 were used as antigens, and guinea pig hyperimmune sera obtained were tested for specific antibody in passive hemagglutination (PH), passive hemagglutination inhibition (PHI) and passive immune hemolysis (PIL) assays. Fresh and SRBC stored in Alsever's solution showed similar behavior when used as indicator cells. Optimal sensitization of erythrocytes was achieved using tannic acid 1:20000 and 20 μg of purified virus/ml at pH 7.6. Specificity of the reaction was confirmed by PH and PHI in homologous and heterologous systems. The coupled antigen-antibody complex was sensitive to complement mediated lysis in a PIL test.
author	Pujol, Carlos Alberto Massouh, Ernesto Jorge
author_facet	Pujol, Carlos Alberto Massouh, Ernesto Jorge
author_sort	Pujol, Carlos Alberto
title	Coupling of foot-and-mouth disease virus to sheep red blood cells using tannic acid for immunological assays
title_short	Coupling of foot-and-mouth disease virus to sheep red blood cells using tannic acid for immunological assays
title_full	Coupling of foot-and-mouth disease virus to sheep red blood cells using tannic acid for immunological assays
title_fullStr	Coupling of foot-and-mouth disease virus to sheep red blood cells using tannic acid for immunological assays
title_full_unstemmed	Coupling of foot-and-mouth disease virus to sheep red blood cells using tannic acid for immunological assays
title_sort	coupling of foot-and-mouth disease virus to sheep red blood cells using tannic acid for immunological assays
publishDate	1988
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01660934_v20_n4_p275_Pujol http://hdl.handle.net/20.500.12110/paper_01660934_v20_n4_p275_Pujol
work_keys_str_mv	AT pujolcarlosalberto couplingoffootandmouthdiseasevirustosheepredbloodcellsusingtannicacidforimmunologicalassays AT massouhernestojorge couplingoffootandmouthdiseasevirustosheepredbloodcellsusingtannicacidforimmunologicalassays
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Coupling of foot-and-mouth disease virus to sheep red blood cells using tannic acid for immunological assays

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