Oral administration of one dose of cholera toxin induces a systemic immune response prior to a mucosal immune response by a direct presentation in the spleen
In the present report the results indicate that the oral administration of one dose of CT in rats results in an antibody immune response in the spleen 48 h later, whereas no antitoxin antibody forming cells were found in the Peyer patches (PP), mesenteric lymph node (MLN) and lamina propria (LP) of...
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1998
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01652478_v60_n2-3_p149_Benedetti http://hdl.handle.net/20.500.12110/paper_01652478_v60_n2-3_p149_Benedetti |
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paper:paper_01652478_v60_n2-3_p149_Benedetti2023-06-08T15:14:51Z Oral administration of one dose of cholera toxin induces a systemic immune response prior to a mucosal immune response by a direct presentation in the spleen Antigen presenting cells Cholera toxin Oral immunization Primary immune response cholera toxin immunoglobulin a antibody immunoglobulin g antibody immunoglobulin m antibody animal cell animal model antibody response antigen presenting cell article cholera enzyme linked immunosorbent assay gastrointestinal infection humoral immunity immunization lymphocyte culture mesentery lymph node nonhuman oral drug administration peyer patch priority journal rat spleen cell Administration, Oral Animals Antibodies, Bacterial Antigen-Presenting Cells Antitoxins Cholera Toxin Immunity, Mucosal Kinetics Rats Rats, Wistar Spleen Time Factors Vibrio cholerae In the present report the results indicate that the oral administration of one dose of CT in rats results in an antibody immune response in the spleen 48 h later, whereas no antitoxin antibody forming cells were found in the Peyer patches (PP), mesenteric lymph node (MLN) and lamina propria (LP) of the small intestine. At this time the main isotype of the antitoxin antibodies in the spleen were IgG and IgM, 5 days after the priming, few antitoxin AFC were observed in the MLN, IgG being the main isotype, whereas no IgM antitoxin AFC were found. At 1 week after priming the number of antitoxin AFC in the MLN reached similar values to those observed in the spleen. When cells from the spleen of rats primed orally with one dose of CT were cultured during 4 days in the presence of inhibitory doses of anti-Ia MAb (OX6), the number of antitoxin AFC was diminished when compared with that observed when cells were cultured in the absence of anti-Ia. The main isotype of antitoxin AFC observed when cells were analyzed after culture was IgM and it was the isotype most affected by the treatment with MAb anti-Ia. These results strongly suggest that an in situ presentation of the antigen did occur in the spleen. On the other hand, when the secondary immune response was studied 48 h after boosting, antitoxin AFC were found in the PP, MLN, SP and LP and 5 days after the booster a 20-30-fold increase was observed in all lymphoid tissues studied, indicating that the secondary immune response found in the spleen was mainly due to the recruitment of memory cells from Peyer's patches. However, when spleen cells were cultured 48 h after the immunization in the presence of inhibitory doses of anti-Ia a little decrease in the number of AFC was observed when compared with the controls (in absence of anti-Ia). The analysis of the antitoxin antibodies in sera and intestinal fluids were in line with the results presented above. The results shown in this report indicate that the systemic immune response observed after the oral administration of CT could be due in part to an in situ presentation of the antigen in the systemic compartments, especially in the spleen. 1998 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01652478_v60_n2-3_p149_Benedetti http://hdl.handle.net/20.500.12110/paper_01652478_v60_n2-3_p149_Benedetti |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Antigen presenting cells Cholera toxin Oral immunization Primary immune response cholera toxin immunoglobulin a antibody immunoglobulin g antibody immunoglobulin m antibody animal cell animal model antibody response antigen presenting cell article cholera enzyme linked immunosorbent assay gastrointestinal infection humoral immunity immunization lymphocyte culture mesentery lymph node nonhuman oral drug administration peyer patch priority journal rat spleen cell Administration, Oral Animals Antibodies, Bacterial Antigen-Presenting Cells Antitoxins Cholera Toxin Immunity, Mucosal Kinetics Rats Rats, Wistar Spleen Time Factors Vibrio cholerae |
| spellingShingle |
Antigen presenting cells Cholera toxin Oral immunization Primary immune response cholera toxin immunoglobulin a antibody immunoglobulin g antibody immunoglobulin m antibody animal cell animal model antibody response antigen presenting cell article cholera enzyme linked immunosorbent assay gastrointestinal infection humoral immunity immunization lymphocyte culture mesentery lymph node nonhuman oral drug administration peyer patch priority journal rat spleen cell Administration, Oral Animals Antibodies, Bacterial Antigen-Presenting Cells Antitoxins Cholera Toxin Immunity, Mucosal Kinetics Rats Rats, Wistar Spleen Time Factors Vibrio cholerae Oral administration of one dose of cholera toxin induces a systemic immune response prior to a mucosal immune response by a direct presentation in the spleen |
| topic_facet |
Antigen presenting cells Cholera toxin Oral immunization Primary immune response cholera toxin immunoglobulin a antibody immunoglobulin g antibody immunoglobulin m antibody animal cell animal model antibody response antigen presenting cell article cholera enzyme linked immunosorbent assay gastrointestinal infection humoral immunity immunization lymphocyte culture mesentery lymph node nonhuman oral drug administration peyer patch priority journal rat spleen cell Administration, Oral Animals Antibodies, Bacterial Antigen-Presenting Cells Antitoxins Cholera Toxin Immunity, Mucosal Kinetics Rats Rats, Wistar Spleen Time Factors Vibrio cholerae |
| description |
In the present report the results indicate that the oral administration of one dose of CT in rats results in an antibody immune response in the spleen 48 h later, whereas no antitoxin antibody forming cells were found in the Peyer patches (PP), mesenteric lymph node (MLN) and lamina propria (LP) of the small intestine. At this time the main isotype of the antitoxin antibodies in the spleen were IgG and IgM, 5 days after the priming, few antitoxin AFC were observed in the MLN, IgG being the main isotype, whereas no IgM antitoxin AFC were found. At 1 week after priming the number of antitoxin AFC in the MLN reached similar values to those observed in the spleen. When cells from the spleen of rats primed orally with one dose of CT were cultured during 4 days in the presence of inhibitory doses of anti-Ia MAb (OX6), the number of antitoxin AFC was diminished when compared with that observed when cells were cultured in the absence of anti-Ia. The main isotype of antitoxin AFC observed when cells were analyzed after culture was IgM and it was the isotype most affected by the treatment with MAb anti-Ia. These results strongly suggest that an in situ presentation of the antigen did occur in the spleen. On the other hand, when the secondary immune response was studied 48 h after boosting, antitoxin AFC were found in the PP, MLN, SP and LP and 5 days after the booster a 20-30-fold increase was observed in all lymphoid tissues studied, indicating that the secondary immune response found in the spleen was mainly due to the recruitment of memory cells from Peyer's patches. However, when spleen cells were cultured 48 h after the immunization in the presence of inhibitory doses of anti-Ia a little decrease in the number of AFC was observed when compared with the controls (in absence of anti-Ia). The analysis of the antitoxin antibodies in sera and intestinal fluids were in line with the results presented above. The results shown in this report indicate that the systemic immune response observed after the oral administration of CT could be due in part to an in situ presentation of the antigen in the systemic compartments, especially in the spleen. |
| title |
Oral administration of one dose of cholera toxin induces a systemic immune response prior to a mucosal immune response by a direct presentation in the spleen |
| title_short |
Oral administration of one dose of cholera toxin induces a systemic immune response prior to a mucosal immune response by a direct presentation in the spleen |
| title_full |
Oral administration of one dose of cholera toxin induces a systemic immune response prior to a mucosal immune response by a direct presentation in the spleen |
| title_fullStr |
Oral administration of one dose of cholera toxin induces a systemic immune response prior to a mucosal immune response by a direct presentation in the spleen |
| title_full_unstemmed |
Oral administration of one dose of cholera toxin induces a systemic immune response prior to a mucosal immune response by a direct presentation in the spleen |
| title_sort |
oral administration of one dose of cholera toxin induces a systemic immune response prior to a mucosal immune response by a direct presentation in the spleen |
| publishDate |
1998 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01652478_v60_n2-3_p149_Benedetti http://hdl.handle.net/20.500.12110/paper_01652478_v60_n2-3_p149_Benedetti |
| _version_ |
1768545921331625984 |