Identity of the major cysteine proteinase (cruzipain) from Trypanosoma cruzi and an antigen (Ag163B6) isolated with a monoclonal antibody
Cruzipain, purified by conventional methods, and Ag163B6, isolated by affinity chromatography with a monoclonal antibody raised against a T. cruzi extract, are glycoproteins with a similar electrophoretic mobility, which reacted with sera from most chronic chagasic patients. Their behaviour in SDS-P...
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Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01652478_v35_n1_p59_Malchiodi http://hdl.handle.net/20.500.12110/paper_01652478_v35_n1_p59_Malchiodi |
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paper:paper_01652478_v35_n1_p59_Malchiodi2023-06-08T15:14:50Z Identity of the major cysteine proteinase (cruzipain) from Trypanosoma cruzi and an antigen (Ag163B6) isolated with a monoclonal antibody Martinez, Javier Augusto Ag163B6 Cruzipain monoclonal antibody parasite antigen affinity chromatography article mouse nonhuman priority journal trypanosoma cruzi Animal Antibodies, Monoclonal Antigens, Protozoan Blotting, Western Cysteine Endopeptidases Electrophoresis, Gel, Two-Dimensional Immunodiffusion Isoelectric Focusing Support, Non-U.S. Gov't Trypanosoma cruzi Cruzipain, purified by conventional methods, and Ag163B6, isolated by affinity chromatography with a monoclonal antibody raised against a T. cruzi extract, are glycoproteins with a similar electrophoretic mobility, which reacted with sera from most chronic chagasic patients. Their behaviour in SDS-PAGE, Western blotting, isoelectric focusing, two-dimensional electrophoresis (IEF and SDS-PAGE), Ouchterlony's double diffusion, and enzyme activity in SDS-PAGE gels containing 0.1% gelatin suggests that they are identical. © 1993. Fil:Martinez, J.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1993 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01652478_v35_n1_p59_Malchiodi http://hdl.handle.net/20.500.12110/paper_01652478_v35_n1_p59_Malchiodi |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
Ag163B6 Cruzipain monoclonal antibody parasite antigen affinity chromatography article mouse nonhuman priority journal trypanosoma cruzi Animal Antibodies, Monoclonal Antigens, Protozoan Blotting, Western Cysteine Endopeptidases Electrophoresis, Gel, Two-Dimensional Immunodiffusion Isoelectric Focusing Support, Non-U.S. Gov't Trypanosoma cruzi |
spellingShingle |
Ag163B6 Cruzipain monoclonal antibody parasite antigen affinity chromatography article mouse nonhuman priority journal trypanosoma cruzi Animal Antibodies, Monoclonal Antigens, Protozoan Blotting, Western Cysteine Endopeptidases Electrophoresis, Gel, Two-Dimensional Immunodiffusion Isoelectric Focusing Support, Non-U.S. Gov't Trypanosoma cruzi Martinez, Javier Augusto Identity of the major cysteine proteinase (cruzipain) from Trypanosoma cruzi and an antigen (Ag163B6) isolated with a monoclonal antibody |
topic_facet |
Ag163B6 Cruzipain monoclonal antibody parasite antigen affinity chromatography article mouse nonhuman priority journal trypanosoma cruzi Animal Antibodies, Monoclonal Antigens, Protozoan Blotting, Western Cysteine Endopeptidases Electrophoresis, Gel, Two-Dimensional Immunodiffusion Isoelectric Focusing Support, Non-U.S. Gov't Trypanosoma cruzi |
description |
Cruzipain, purified by conventional methods, and Ag163B6, isolated by affinity chromatography with a monoclonal antibody raised against a T. cruzi extract, are glycoproteins with a similar electrophoretic mobility, which reacted with sera from most chronic chagasic patients. Their behaviour in SDS-PAGE, Western blotting, isoelectric focusing, two-dimensional electrophoresis (IEF and SDS-PAGE), Ouchterlony's double diffusion, and enzyme activity in SDS-PAGE gels containing 0.1% gelatin suggests that they are identical. © 1993. |
author |
Martinez, Javier Augusto |
author_facet |
Martinez, Javier Augusto |
author_sort |
Martinez, Javier Augusto |
title |
Identity of the major cysteine proteinase (cruzipain) from Trypanosoma cruzi and an antigen (Ag163B6) isolated with a monoclonal antibody |
title_short |
Identity of the major cysteine proteinase (cruzipain) from Trypanosoma cruzi and an antigen (Ag163B6) isolated with a monoclonal antibody |
title_full |
Identity of the major cysteine proteinase (cruzipain) from Trypanosoma cruzi and an antigen (Ag163B6) isolated with a monoclonal antibody |
title_fullStr |
Identity of the major cysteine proteinase (cruzipain) from Trypanosoma cruzi and an antigen (Ag163B6) isolated with a monoclonal antibody |
title_full_unstemmed |
Identity of the major cysteine proteinase (cruzipain) from Trypanosoma cruzi and an antigen (Ag163B6) isolated with a monoclonal antibody |
title_sort |
identity of the major cysteine proteinase (cruzipain) from trypanosoma cruzi and an antigen (ag163b6) isolated with a monoclonal antibody |
publishDate |
1993 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01652478_v35_n1_p59_Malchiodi http://hdl.handle.net/20.500.12110/paper_01652478_v35_n1_p59_Malchiodi |
work_keys_str_mv |
AT martinezjavieraugusto identityofthemajorcysteineproteinasecruzipainfromtrypanosomacruziandanantigenag163b6isolatedwithamonoclonalantibody |
_version_ |
1768546718646796288 |