Anti-tissue transglutaminase antibody inhibits apoptotic cell clearance by macrophages in pregnant NOD mice

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Autoimmunity is a feature of celiac disease (CD) with tissue transglutaminase (tTG) as a major autoantigen. A correlation between gynecological-obstetric disorders in CD patients and the presence of circulating antibodies anti-tTG that inhibited tTG activity was reported. Serum anti-tTG antibodies w...

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Autores principales:	Hauk, Vanesa Cintia, Ramhorst, Rosanna Elizabeth
Publicado:	2014
Materias:	Anti-tissue transglutaminase antibodies Celiac disease NOD mice autoantibody immunoglobulin A immunoglobulin G immunoglobulin G antibody protein glutamine gamma glutamyltransferase protein glutamine gamma glutamyltransferase antibody antibody autoantigen guanine nucleotide binding protein protein glutamine gamma glutamyltransferase 2 animal cell animal experiment apoptosis article celiac disease cell phagocytosis controlled study enzyme activity enzyme linked immunosorbent assay extracellular matrix female first trimester pregnancy immunofluorescence immunohistochemistry male mouse nonhuman nonobese diabetic mouse peritoneum macrophage pregnancy priority journal protein expression animal Bagg albino mouse blood immunology insulin dependent diabetes mellitus macrophage phagocytosis pregnancy outcome Sjoegren syndrome uterus Animals Antibodies Apoptosis Autoantigens Celiac Disease Diabetes Mellitus, Type 1 Female GTP-Binding Proteins Immunoglobulin G Macrophages Mice Mice, Inbred BALB C Mice, Inbred NOD Phagocytosis Pregnancy Pregnancy Outcome Sjogren's Syndrome Transglutaminases Uterus
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01650378_v103_n1_p59_Sonora http://hdl.handle.net/20.500.12110/paper_01650378_v103_n1_p59_Sonora
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_01650378_v103_n1_p59_Sonora
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spelling	paper:paper_01650378_v103_n1_p59_Sonora2023-06-08T15:14:38Z Anti-tissue transglutaminase antibody inhibits apoptotic cell clearance by macrophages in pregnant NOD mice Hauk, Vanesa Cintia Ramhorst, Rosanna Elizabeth Anti-tissue transglutaminase antibodies Celiac disease NOD mice autoantibody immunoglobulin A immunoglobulin G immunoglobulin G antibody protein glutamine gamma glutamyltransferase protein glutamine gamma glutamyltransferase antibody antibody autoantigen guanine nucleotide binding protein immunoglobulin G protein glutamine gamma glutamyltransferase protein glutamine gamma glutamyltransferase 2 animal cell animal experiment apoptosis article celiac disease cell phagocytosis controlled study enzyme activity enzyme linked immunosorbent assay extracellular matrix female first trimester pregnancy immunofluorescence immunohistochemistry male mouse nonhuman nonobese diabetic mouse peritoneum macrophage pregnancy priority journal protein expression animal apoptosis Bagg albino mouse blood immunology insulin dependent diabetes mellitus macrophage phagocytosis pregnancy pregnancy outcome Sjoegren syndrome uterus Animals Antibodies Apoptosis Autoantigens Celiac Disease Diabetes Mellitus, Type 1 Female GTP-Binding Proteins Immunoglobulin G Macrophages Mice Mice, Inbred BALB C Mice, Inbred NOD Phagocytosis Pregnancy Pregnancy Outcome Sjogren's Syndrome Transglutaminases Uterus Autoimmunity is a feature of celiac disease (CD) with tissue transglutaminase (tTG) as a major autoantigen. A correlation between gynecological-obstetric disorders in CD patients and the presence of circulating antibodies anti-tTG that inhibited tTG activity was reported. Serum anti-tTG antibodies were detected in a non-obese diabetic (NOD) mouse model of type I insulin-dependent diabetes mellitus and Sjögren's syndrome, two comorbid states with CD. Since pregnancy complications have been described in NOD mice, we evaluated the ability of anti-tTG antibodies to affect the functions of tTG relevant to the normal course of an early pregnancy like extracellular matrix assembling and apoptotic cell phagocytosis by macrophages. Circulating IgG antibodies against tTG were detected in NOD mice with titers that decreased at early pregnancy; interestingly, the in vitro transamidating activity of tTG was reduced by NOD serum samples. Particularly, anti-tTG antibody inhibited apoptotic cell phagocytosis by peritoneal macrophages from pregnant NOD mice that express the enzyme on surface. Evidence provided support for a role for anti-tTG antibodies through reduced transamidating activity and reduced apoptotic cell clearance by the macrophages of pregnant NOD mice. © 2013 Elsevier Ireland Ltd. Fil:Hauk, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ramhorst, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2014 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01650378_v103_n1_p59_Sonora http://hdl.handle.net/20.500.12110/paper_01650378_v103_n1_p59_Sonora
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Anti-tissue transglutaminase antibodies Celiac disease NOD mice autoantibody immunoglobulin A immunoglobulin G immunoglobulin G antibody protein glutamine gamma glutamyltransferase protein glutamine gamma glutamyltransferase antibody antibody autoantigen guanine nucleotide binding protein immunoglobulin G protein glutamine gamma glutamyltransferase protein glutamine gamma glutamyltransferase 2 animal cell animal experiment apoptosis article celiac disease cell phagocytosis controlled study enzyme activity enzyme linked immunosorbent assay extracellular matrix female first trimester pregnancy immunofluorescence immunohistochemistry male mouse nonhuman nonobese diabetic mouse peritoneum macrophage pregnancy priority journal protein expression animal apoptosis Bagg albino mouse blood immunology insulin dependent diabetes mellitus macrophage phagocytosis pregnancy pregnancy outcome Sjoegren syndrome uterus Animals Antibodies Apoptosis Autoantigens Celiac Disease Diabetes Mellitus, Type 1 Female GTP-Binding Proteins Immunoglobulin G Macrophages Mice Mice, Inbred BALB C Mice, Inbred NOD Phagocytosis Pregnancy Pregnancy Outcome Sjogren's Syndrome Transglutaminases Uterus
spellingShingle	Anti-tissue transglutaminase antibodies Celiac disease NOD mice autoantibody immunoglobulin A immunoglobulin G immunoglobulin G antibody protein glutamine gamma glutamyltransferase protein glutamine gamma glutamyltransferase antibody antibody autoantigen guanine nucleotide binding protein immunoglobulin G protein glutamine gamma glutamyltransferase protein glutamine gamma glutamyltransferase 2 animal cell animal experiment apoptosis article celiac disease cell phagocytosis controlled study enzyme activity enzyme linked immunosorbent assay extracellular matrix female first trimester pregnancy immunofluorescence immunohistochemistry male mouse nonhuman nonobese diabetic mouse peritoneum macrophage pregnancy priority journal protein expression animal apoptosis Bagg albino mouse blood immunology insulin dependent diabetes mellitus macrophage phagocytosis pregnancy pregnancy outcome Sjoegren syndrome uterus Animals Antibodies Apoptosis Autoantigens Celiac Disease Diabetes Mellitus, Type 1 Female GTP-Binding Proteins Immunoglobulin G Macrophages Mice Mice, Inbred BALB C Mice, Inbred NOD Phagocytosis Pregnancy Pregnancy Outcome Sjogren's Syndrome Transglutaminases Uterus Hauk, Vanesa Cintia Ramhorst, Rosanna Elizabeth Anti-tissue transglutaminase antibody inhibits apoptotic cell clearance by macrophages in pregnant NOD mice
topic_facet	Anti-tissue transglutaminase antibodies Celiac disease NOD mice autoantibody immunoglobulin A immunoglobulin G immunoglobulin G antibody protein glutamine gamma glutamyltransferase protein glutamine gamma glutamyltransferase antibody antibody autoantigen guanine nucleotide binding protein immunoglobulin G protein glutamine gamma glutamyltransferase protein glutamine gamma glutamyltransferase 2 animal cell animal experiment apoptosis article celiac disease cell phagocytosis controlled study enzyme activity enzyme linked immunosorbent assay extracellular matrix female first trimester pregnancy immunofluorescence immunohistochemistry male mouse nonhuman nonobese diabetic mouse peritoneum macrophage pregnancy priority journal protein expression animal apoptosis Bagg albino mouse blood immunology insulin dependent diabetes mellitus macrophage phagocytosis pregnancy pregnancy outcome Sjoegren syndrome uterus Animals Antibodies Apoptosis Autoantigens Celiac Disease Diabetes Mellitus, Type 1 Female GTP-Binding Proteins Immunoglobulin G Macrophages Mice Mice, Inbred BALB C Mice, Inbred NOD Phagocytosis Pregnancy Pregnancy Outcome Sjogren's Syndrome Transglutaminases Uterus
description	Autoimmunity is a feature of celiac disease (CD) with tissue transglutaminase (tTG) as a major autoantigen. A correlation between gynecological-obstetric disorders in CD patients and the presence of circulating antibodies anti-tTG that inhibited tTG activity was reported. Serum anti-tTG antibodies were detected in a non-obese diabetic (NOD) mouse model of type I insulin-dependent diabetes mellitus and Sjögren's syndrome, two comorbid states with CD. Since pregnancy complications have been described in NOD mice, we evaluated the ability of anti-tTG antibodies to affect the functions of tTG relevant to the normal course of an early pregnancy like extracellular matrix assembling and apoptotic cell phagocytosis by macrophages. Circulating IgG antibodies against tTG were detected in NOD mice with titers that decreased at early pregnancy; interestingly, the in vitro transamidating activity of tTG was reduced by NOD serum samples. Particularly, anti-tTG antibody inhibited apoptotic cell phagocytosis by peritoneal macrophages from pregnant NOD mice that express the enzyme on surface. Evidence provided support for a role for anti-tTG antibodies through reduced transamidating activity and reduced apoptotic cell clearance by the macrophages of pregnant NOD mice. © 2013 Elsevier Ireland Ltd.
author	Hauk, Vanesa Cintia Ramhorst, Rosanna Elizabeth
author_facet	Hauk, Vanesa Cintia Ramhorst, Rosanna Elizabeth
author_sort	Hauk, Vanesa Cintia
title	Anti-tissue transglutaminase antibody inhibits apoptotic cell clearance by macrophages in pregnant NOD mice
title_short	Anti-tissue transglutaminase antibody inhibits apoptotic cell clearance by macrophages in pregnant NOD mice
title_full	Anti-tissue transglutaminase antibody inhibits apoptotic cell clearance by macrophages in pregnant NOD mice
title_fullStr	Anti-tissue transglutaminase antibody inhibits apoptotic cell clearance by macrophages in pregnant NOD mice
title_full_unstemmed	Anti-tissue transglutaminase antibody inhibits apoptotic cell clearance by macrophages in pregnant NOD mice
title_sort	anti-tissue transglutaminase antibody inhibits apoptotic cell clearance by macrophages in pregnant nod mice
publishDate	2014
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01650378_v103_n1_p59_Sonora http://hdl.handle.net/20.500.12110/paper_01650378_v103_n1_p59_Sonora
work_keys_str_mv	AT haukvanesacintia antitissuetransglutaminaseantibodyinhibitsapoptoticcellclearancebymacrophagesinpregnantnodmice AT ramhorstrosannaelizabeth antitissuetransglutaminaseantibodyinhibitsapoptoticcellclearancebymacrophagesinpregnantnodmice
_version_	1768543510186688512

Anti-tissue transglutaminase antibody inhibits apoptotic cell clearance by macrophages in pregnant NOD mice

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