New methods for spectrofluorometer monochromator wavelength calibration
A method is presented for wavelength calibration of spectrofluorometer monochromators. It is based on the distortion that the characteristics absorption bands of glass filters (holmium or didymium oxide), commonly used for calibration of spectrophotometers, introduce in the emitted fluorescence of f...
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1988
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0165022X_v17_n1_p61_Paladini http://hdl.handle.net/20.500.12110/paper_0165022X_v17_n1_p61_Paladini |
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paper:paper_0165022X_v17_n1_p61_Paladini2023-06-08T15:14:33Z New methods for spectrofluorometer monochromator wavelength calibration Erijman, Leonardo Calibration Didymium filter Holmium filter Instrumentation Monochrmator Wavelength article calibration instrumentation spectrofluorometry standard Calibration Spectrometry, Fluorescence Weights and Measures A method is presented for wavelength calibration of spectrofluorometer monochromators. It is based on the distortion that the characteristics absorption bands of glass filters (holmium or didymium oxide), commonly used for calibration of spectrophotometers, introduce in the emitted fluorescence of fluorophores like indole, diphenyl hexatriene, xylene or rhodamine 6G. Those filters or a well characterized absorber with sharp bands like benzene vapor can be used for the same purpose. The wavelength calibration accuracy obtained with this method is better than 0.1 nm, and requires no modification in the geometry of the spectrofluorometer sample compartment. © 1988. Fil:Erijman, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1988 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0165022X_v17_n1_p61_Paladini http://hdl.handle.net/20.500.12110/paper_0165022X_v17_n1_p61_Paladini |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Calibration Didymium filter Holmium filter Instrumentation Monochrmator Wavelength article calibration instrumentation spectrofluorometry standard Calibration Spectrometry, Fluorescence Weights and Measures |
| spellingShingle |
Calibration Didymium filter Holmium filter Instrumentation Monochrmator Wavelength article calibration instrumentation spectrofluorometry standard Calibration Spectrometry, Fluorescence Weights and Measures Erijman, Leonardo New methods for spectrofluorometer monochromator wavelength calibration |
| topic_facet |
Calibration Didymium filter Holmium filter Instrumentation Monochrmator Wavelength article calibration instrumentation spectrofluorometry standard Calibration Spectrometry, Fluorescence Weights and Measures |
| description |
A method is presented for wavelength calibration of spectrofluorometer monochromators. It is based on the distortion that the characteristics absorption bands of glass filters (holmium or didymium oxide), commonly used for calibration of spectrophotometers, introduce in the emitted fluorescence of fluorophores like indole, diphenyl hexatriene, xylene or rhodamine 6G. Those filters or a well characterized absorber with sharp bands like benzene vapor can be used for the same purpose. The wavelength calibration accuracy obtained with this method is better than 0.1 nm, and requires no modification in the geometry of the spectrofluorometer sample compartment. © 1988. |
| author |
Erijman, Leonardo |
| author_facet |
Erijman, Leonardo |
| author_sort |
Erijman, Leonardo |
| title |
New methods for spectrofluorometer monochromator wavelength calibration |
| title_short |
New methods for spectrofluorometer monochromator wavelength calibration |
| title_full |
New methods for spectrofluorometer monochromator wavelength calibration |
| title_fullStr |
New methods for spectrofluorometer monochromator wavelength calibration |
| title_full_unstemmed |
New methods for spectrofluorometer monochromator wavelength calibration |
| title_sort |
new methods for spectrofluorometer monochromator wavelength calibration |
| publishDate |
1988 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0165022X_v17_n1_p61_Paladini http://hdl.handle.net/20.500.12110/paper_0165022X_v17_n1_p61_Paladini |
| work_keys_str_mv |
AT erijmanleonardo newmethodsforspectrofluorometermonochromatorwavelengthcalibration |
| _version_ |
1768542783913590784 |