Colchicine blocks β adrenoceptor and class I antigen-specific interactions
Previously we have demonstrated a molecular relationship between H-2 class I antigens and β adrenoceptors from cardiac tissue. Here we show this type of interaction taking place with β adrenoceptors from splenic cells and their purified membranes and the participation of cytoskeletal proteins in the...
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1989
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01615890_v26_n7_p601_Cremaschi http://hdl.handle.net/20.500.12110/paper_01615890_v26_n7_p601_Cremaschi |
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paper:paper_01615890_v26_n7_p601_Cremaschi |
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paper:paper_01615890_v26_n7_p601_Cremaschi2023-06-08T15:13:29Z Colchicine blocks β adrenoceptor and class I antigen-specific interactions alloantibody alloantigens colchicine cytoskeletal proteins β adrenoceptor alloantibody beta adrenergic receptor colchicine cyclic amp cytoskeleton protein hormone receptor immunoglobulin g major histocompatibility antigen class 1 animal cell animal experiment biological model cell culture cytochemistry heart immunization lymphocyte mouse nonhuman priority journal spleen cell Animal Binding, Competitive Colchicine Cyclic AMP Cytochalasin B H-2 Antigens Immunoglobulin G Isoantibodies Mice Mice, Inbred BALB C Mice, Inbred C3H Receptors, Adrenergic, beta Spleen Support, Non-U.S. Gov't Previously we have demonstrated a molecular relationship between H-2 class I antigens and β adrenoceptors from cardiac tissue. Here we show this type of interaction taking place with β adrenoceptors from splenic cells and their purified membranes and the participation of cytoskeletal proteins in the phenomenon. Alloimmune, as well as anti-class I but not anti-class II, antibodies were able to inhibit in a competitive manner the binding of (-)-[3 H]dihydroalprenolol to splenic lymphocytes and their purified membranes, and to increase cyclic AMP levels in intact cells as a consequence of β adrenoceptor activation. Furthermore, colchicine (a microtubule disrupting drug), but not cytochalasine B (a microfilament disrupting drug), was able to abrogate alloimmune antibody inhibition over the β radioligand binding to its receptor on both intact splenocytes and their membranes. Alloantibody actions were significantly diminished by peripheral protein solubilization in purified spleen cell membranes. These data pointed indirectly to the participation of a colchicine binding protein in class I antigen and hormone-receptor associations. © 1989. 1989 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01615890_v26_n7_p601_Cremaschi http://hdl.handle.net/20.500.12110/paper_01615890_v26_n7_p601_Cremaschi |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
alloantibody alloantigens colchicine cytoskeletal proteins β adrenoceptor alloantibody beta adrenergic receptor colchicine cyclic amp cytoskeleton protein hormone receptor immunoglobulin g major histocompatibility antigen class 1 animal cell animal experiment biological model cell culture cytochemistry heart immunization lymphocyte mouse nonhuman priority journal spleen cell Animal Binding, Competitive Colchicine Cyclic AMP Cytochalasin B H-2 Antigens Immunoglobulin G Isoantibodies Mice Mice, Inbred BALB C Mice, Inbred C3H Receptors, Adrenergic, beta Spleen Support, Non-U.S. Gov't |
| spellingShingle |
alloantibody alloantigens colchicine cytoskeletal proteins β adrenoceptor alloantibody beta adrenergic receptor colchicine cyclic amp cytoskeleton protein hormone receptor immunoglobulin g major histocompatibility antigen class 1 animal cell animal experiment biological model cell culture cytochemistry heart immunization lymphocyte mouse nonhuman priority journal spleen cell Animal Binding, Competitive Colchicine Cyclic AMP Cytochalasin B H-2 Antigens Immunoglobulin G Isoantibodies Mice Mice, Inbred BALB C Mice, Inbred C3H Receptors, Adrenergic, beta Spleen Support, Non-U.S. Gov't Colchicine blocks β adrenoceptor and class I antigen-specific interactions |
| topic_facet |
alloantibody alloantigens colchicine cytoskeletal proteins β adrenoceptor alloantibody beta adrenergic receptor colchicine cyclic amp cytoskeleton protein hormone receptor immunoglobulin g major histocompatibility antigen class 1 animal cell animal experiment biological model cell culture cytochemistry heart immunization lymphocyte mouse nonhuman priority journal spleen cell Animal Binding, Competitive Colchicine Cyclic AMP Cytochalasin B H-2 Antigens Immunoglobulin G Isoantibodies Mice Mice, Inbred BALB C Mice, Inbred C3H Receptors, Adrenergic, beta Spleen Support, Non-U.S. Gov't |
| description |
Previously we have demonstrated a molecular relationship between H-2 class I antigens and β adrenoceptors from cardiac tissue. Here we show this type of interaction taking place with β adrenoceptors from splenic cells and their purified membranes and the participation of cytoskeletal proteins in the phenomenon. Alloimmune, as well as anti-class I but not anti-class II, antibodies were able to inhibit in a competitive manner the binding of (-)-[3 H]dihydroalprenolol to splenic lymphocytes and their purified membranes, and to increase cyclic AMP levels in intact cells as a consequence of β adrenoceptor activation. Furthermore, colchicine (a microtubule disrupting drug), but not cytochalasine B (a microfilament disrupting drug), was able to abrogate alloimmune antibody inhibition over the β radioligand binding to its receptor on both intact splenocytes and their membranes. Alloantibody actions were significantly diminished by peripheral protein solubilization in purified spleen cell membranes. These data pointed indirectly to the participation of a colchicine binding protein in class I antigen and hormone-receptor associations. © 1989. |
| title |
Colchicine blocks β adrenoceptor and class I antigen-specific interactions |
| title_short |
Colchicine blocks β adrenoceptor and class I antigen-specific interactions |
| title_full |
Colchicine blocks β adrenoceptor and class I antigen-specific interactions |
| title_fullStr |
Colchicine blocks β adrenoceptor and class I antigen-specific interactions |
| title_full_unstemmed |
Colchicine blocks β adrenoceptor and class I antigen-specific interactions |
| title_sort |
colchicine blocks β adrenoceptor and class i antigen-specific interactions |
| publishDate |
1989 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01615890_v26_n7_p601_Cremaschi http://hdl.handle.net/20.500.12110/paper_01615890_v26_n7_p601_Cremaschi |
| _version_ |
1768545323471339520 |