Genotyping of Leptospira interrogans strains from Argentina by Multiple-Locus Variable-number tandem repeat Analysis (MLVA)

Leptospirosis outbreaks occur regularly in Argentina and other South American countries, but little is known about their epidemiological relationships. Application of new molecular tools, such as the Multiple-Locus Variable-number tandem repeat Analysis (MLVA) is limited by scant available data on r...

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Detalles Bibliográficos
Autor principal: Pettinari, María Julia
Publicado: 2011
Materias:
Genotyping
Leptospira interrogans
Molecular diversity
Multiple-Locus Variable-number tandem repeat Analysis (MLVA)
Argentina
article
bacterial strain
bacterium isolate
copy number variation
gene cluster
genetic analysis
genetic variability
genotype
geographic distribution
molecular phylogeny
multiple locus variable number of tandem repeat analysis
nonhuman
nucleotide sequence
sequence analysis
Animals
Base Sequence
Genetic Variation
Genotype
Humans
Leptospirosis
Minisatellite Repeats
Molecular Sequence Data
Multilocus Sequence Typing
Phylogeny
Animalia
Didelphidae
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01479571_v34_n2_p135_Pavan
http://hdl.handle.net/20.500.12110/paper_01479571_v34_n2_p135_Pavan
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_01479571_v34_n2_p135_Pavan
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spelling paper:paper_01479571_v34_n2_p135_Pavan2023-06-08T15:13:01Z Genotyping of Leptospira interrogans strains from Argentina by Multiple-Locus Variable-number tandem repeat Analysis (MLVA) Pettinari, María Julia Genotyping Leptospira interrogans Molecular diversity Multiple-Locus Variable-number tandem repeat Analysis (MLVA) Argentina article bacterial strain bacterium isolate copy number variation gene cluster genetic analysis genetic variability genotype geographic distribution Leptospira interrogans molecular phylogeny multiple locus variable number of tandem repeat analysis nonhuman nucleotide sequence sequence analysis Animals Argentina Base Sequence Genetic Variation Genotype Humans Leptospira interrogans Leptospirosis Minisatellite Repeats Molecular Sequence Data Multilocus Sequence Typing Phylogeny Animalia Didelphidae Leptospira interrogans Leptospirosis outbreaks occur regularly in Argentina and other South American countries, but little is known about their epidemiological relationships. Application of new molecular tools, such as the Multiple-Locus Variable-number tandem repeat Analysis (MLVA) is limited by scant available data on regional strains. We have analyzed the genetic diversity of a collection of 31 strains of Leptospira interrogans isolated in Argentina during the past five decades from humans and animals, including a strain from an environmental source and another isolated from an opossum. Genotyping was performed by MLVA using the loci VNTR4, VNTR7, VNTR9, VNTR10, VNTR19, VNTR23 and VNTR31, as described by Majed et al. [Identification of variable-number tandem-repeat loci in Leptospira interrogans sensu stricto. J Clin Microbiol 2005;43:539-45 [1]]. Clustering analysis revealed eight distinct MLVA genotypes, with a dominant one, genotype A. Strains with this genotype were consistently isolated since 1960, representing 55% of the total strains and spanning an extensive geographical distribution. Other seven genotypes were less frequent, and only genotypes A and Hond Utrecht IV were isolated during the last decade. Different kinds of repeat blocks for each VNTR locus were identified by sequence analysis. VNTR copy number differences among genotypes always involved only one of these blocks. MLVA patterns obtained reveal the genetic diversity and relationships between strains, and constitute the framework for the genotyping of leptospires in the region. © 2010 Elsevier Ltd. Fil:Pettinari, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2011 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01479571_v34_n2_p135_Pavan http://hdl.handle.net/20.500.12110/paper_01479571_v34_n2_p135_Pavan
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Genotyping
Leptospira interrogans
Molecular diversity
Multiple-Locus Variable-number tandem repeat Analysis (MLVA)
Argentina
article
bacterial strain
bacterium isolate
copy number variation
gene cluster
genetic analysis
genetic variability
genotype
geographic distribution
Leptospira interrogans
molecular phylogeny
multiple locus variable number of tandem repeat analysis
nonhuman
nucleotide sequence
sequence analysis
Animals
Argentina
Base Sequence
Genetic Variation
Genotype
Humans
Leptospira interrogans
Leptospirosis
Minisatellite Repeats
Molecular Sequence Data
Multilocus Sequence Typing
Phylogeny
Animalia
Didelphidae
Leptospira interrogans
spellingShingle Genotyping
Leptospira interrogans
Molecular diversity
Multiple-Locus Variable-number tandem repeat Analysis (MLVA)
Argentina
article
bacterial strain
bacterium isolate
copy number variation
gene cluster
genetic analysis
genetic variability
genotype
geographic distribution
Leptospira interrogans
molecular phylogeny
multiple locus variable number of tandem repeat analysis
nonhuman
nucleotide sequence
sequence analysis
Animals
Argentina
Base Sequence
Genetic Variation
Genotype
Humans
Leptospira interrogans
Leptospirosis
Minisatellite Repeats
Molecular Sequence Data
Multilocus Sequence Typing
Phylogeny
Animalia
Didelphidae
Leptospira interrogans
Pettinari, María Julia
Genotyping of Leptospira interrogans strains from Argentina by Multiple-Locus Variable-number tandem repeat Analysis (MLVA)
topic_facet Genotyping
Leptospira interrogans
Molecular diversity
Multiple-Locus Variable-number tandem repeat Analysis (MLVA)
Argentina
article
bacterial strain
bacterium isolate
copy number variation
gene cluster
genetic analysis
genetic variability
genotype
geographic distribution
Leptospira interrogans
molecular phylogeny
multiple locus variable number of tandem repeat analysis
nonhuman
nucleotide sequence
sequence analysis
Animals
Argentina
Base Sequence
Genetic Variation
Genotype
Humans
Leptospira interrogans
Leptospirosis
Minisatellite Repeats
Molecular Sequence Data
Multilocus Sequence Typing
Phylogeny
Animalia
Didelphidae
Leptospira interrogans
description Leptospirosis outbreaks occur regularly in Argentina and other South American countries, but little is known about their epidemiological relationships. Application of new molecular tools, such as the Multiple-Locus Variable-number tandem repeat Analysis (MLVA) is limited by scant available data on regional strains. We have analyzed the genetic diversity of a collection of 31 strains of Leptospira interrogans isolated in Argentina during the past five decades from humans and animals, including a strain from an environmental source and another isolated from an opossum. Genotyping was performed by MLVA using the loci VNTR4, VNTR7, VNTR9, VNTR10, VNTR19, VNTR23 and VNTR31, as described by Majed et al. [Identification of variable-number tandem-repeat loci in Leptospira interrogans sensu stricto. J Clin Microbiol 2005;43:539-45 [1]]. Clustering analysis revealed eight distinct MLVA genotypes, with a dominant one, genotype A. Strains with this genotype were consistently isolated since 1960, representing 55% of the total strains and spanning an extensive geographical distribution. Other seven genotypes were less frequent, and only genotypes A and Hond Utrecht IV were isolated during the last decade. Different kinds of repeat blocks for each VNTR locus were identified by sequence analysis. VNTR copy number differences among genotypes always involved only one of these blocks. MLVA patterns obtained reveal the genetic diversity and relationships between strains, and constitute the framework for the genotyping of leptospires in the region. © 2010 Elsevier Ltd.
author Pettinari, María Julia
author_facet Pettinari, María Julia
author_sort Pettinari, María Julia
title Genotyping of Leptospira interrogans strains from Argentina by Multiple-Locus Variable-number tandem repeat Analysis (MLVA)
title_short Genotyping of Leptospira interrogans strains from Argentina by Multiple-Locus Variable-number tandem repeat Analysis (MLVA)
title_full Genotyping of Leptospira interrogans strains from Argentina by Multiple-Locus Variable-number tandem repeat Analysis (MLVA)
title_fullStr Genotyping of Leptospira interrogans strains from Argentina by Multiple-Locus Variable-number tandem repeat Analysis (MLVA)
title_full_unstemmed Genotyping of Leptospira interrogans strains from Argentina by Multiple-Locus Variable-number tandem repeat Analysis (MLVA)
title_sort genotyping of leptospira interrogans strains from argentina by multiple-locus variable-number tandem repeat analysis (mlva)
publishDate 2011
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01479571_v34_n2_p135_Pavan
http://hdl.handle.net/20.500.12110/paper_01479571_v34_n2_p135_Pavan
work_keys_str_mv AT pettinarimariajulia genotypingofleptospirainterrogansstrainsfromargentinabymultiplelocusvariablenumbertandemrepeatanalysismlva
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