Effects of waterborne exposure to the antidepressant fluoxetine on swimming, shoaling and anxiety behaviours of the mosquitofish Gambusia holbrooki
Chemical pollution from pharmaceuticals is increasingly recognized as a major hazard to the aquatic biota. Among the wide variety of pharmaceuticals, fluoxetine (FLX) is one of the most widely prescribed antidepressants, and therefore, it is frequently identified in the aquatic environment. As FLX i...
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2018
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01476513_v163_n_p646_Meijide http://hdl.handle.net/20.500.12110/paper_01476513_v163_n_p646_Meijide |
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paper:paper_01476513_v163_n_p646_Meijide2023-06-08T15:12:54Z Effects of waterborne exposure to the antidepressant fluoxetine on swimming, shoaling and anxiety behaviours of the mosquitofish Gambusia holbrooki Behavioural tests Fluoxetine Gambusia holbrooki Pharmaceutical pollution Poeciliidae Swimming activity fluoxetine antidepressant agent fluoxetine aquatic environment behavioral response cyprinid drug pollution effect pollution exposure swimming testing method adult animal behavior anxiety Article behavior change concentration (parameters) controlled study ecotoxicology environmental exposure female Gambusia holbrooki light dark cycle locomotion nonhuman swimming water analysis animal animal behavior Cyprinodontiformes drug effect physiology swimming toxicity toxicity testing water pollutant Animalia Gambusia affinis Gambusia holbrooki Poeciliidae Vertebrata Animals Antidepressive Agents Behavior, Animal Cyprinodontiformes Female Fluoxetine Swimming Toxicity Tests Water Pollutants, Chemical Chemical pollution from pharmaceuticals is increasingly recognized as a major hazard to the aquatic biota. Among the wide variety of pharmaceuticals, fluoxetine (FLX) is one of the most widely prescribed antidepressants, and therefore, it is frequently identified in the aquatic environment. As FLX is designed to alter human behaviour and many physiological pathways are conserved across vertebrates, this drug may affect the behaviour of fish living in FLX-polluted environments. Here, we exposed groups of female mosquitofish Gambusia holbrooki to waterborne FLX for 14 days, under semi-static conditions with daily renewal of test solutions. Following exposure, we conducted a set of behavioural assays in individual fish, aimed at assessing the effects of FLX on their locomotor activity and behavioural responses. We found that FLX impaired swimming behaviour at high concentrations (25 μg/L and 50 μg/L) but not at low concentrations close to environmental levels (1 μg/L and 5 μg/L). When swimming activity was assessed 5 min after transfer of the focal fish to the testing tank, 50 μg/L FLX was the only concentration showing significant effects. However, when the same trials were performed 24 h later, 25 μg/L FLX turned out to be an effect concentration in addition to 50 μg/L. Interestingly, these concentrations would elicit fish plasma concentrations comprised within the range of human therapeutic doses. When subjected to a light/dark preference test, fish showed tendency to remain less time in the dark area at high FLX concentrations, thus suggesting an anti-anxiety response. Shoaling behaviour was not affected by FLX exposure. Our study contributes to the growing body of literature evaluating the effects of FLX on animal behaviour. Regarding the experimental design used in behavioural testing, our findings suggest that focal fish should be subjected to long habituation periods, namely of at least a few hours, in order to better assess the effects of drug exposure. © 2018 Elsevier Inc. 2018 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01476513_v163_n_p646_Meijide http://hdl.handle.net/20.500.12110/paper_01476513_v163_n_p646_Meijide |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Behavioural tests Fluoxetine Gambusia holbrooki Pharmaceutical pollution Poeciliidae Swimming activity fluoxetine antidepressant agent fluoxetine aquatic environment behavioral response cyprinid drug pollution effect pollution exposure swimming testing method adult animal behavior anxiety Article behavior change concentration (parameters) controlled study ecotoxicology environmental exposure female Gambusia holbrooki light dark cycle locomotion nonhuman swimming water analysis animal animal behavior Cyprinodontiformes drug effect physiology swimming toxicity toxicity testing water pollutant Animalia Gambusia affinis Gambusia holbrooki Poeciliidae Vertebrata Animals Antidepressive Agents Behavior, Animal Cyprinodontiformes Female Fluoxetine Swimming Toxicity Tests Water Pollutants, Chemical |
| spellingShingle |
Behavioural tests Fluoxetine Gambusia holbrooki Pharmaceutical pollution Poeciliidae Swimming activity fluoxetine antidepressant agent fluoxetine aquatic environment behavioral response cyprinid drug pollution effect pollution exposure swimming testing method adult animal behavior anxiety Article behavior change concentration (parameters) controlled study ecotoxicology environmental exposure female Gambusia holbrooki light dark cycle locomotion nonhuman swimming water analysis animal animal behavior Cyprinodontiformes drug effect physiology swimming toxicity toxicity testing water pollutant Animalia Gambusia affinis Gambusia holbrooki Poeciliidae Vertebrata Animals Antidepressive Agents Behavior, Animal Cyprinodontiformes Female Fluoxetine Swimming Toxicity Tests Water Pollutants, Chemical Effects of waterborne exposure to the antidepressant fluoxetine on swimming, shoaling and anxiety behaviours of the mosquitofish Gambusia holbrooki |
| topic_facet |
Behavioural tests Fluoxetine Gambusia holbrooki Pharmaceutical pollution Poeciliidae Swimming activity fluoxetine antidepressant agent fluoxetine aquatic environment behavioral response cyprinid drug pollution effect pollution exposure swimming testing method adult animal behavior anxiety Article behavior change concentration (parameters) controlled study ecotoxicology environmental exposure female Gambusia holbrooki light dark cycle locomotion nonhuman swimming water analysis animal animal behavior Cyprinodontiformes drug effect physiology swimming toxicity toxicity testing water pollutant Animalia Gambusia affinis Gambusia holbrooki Poeciliidae Vertebrata Animals Antidepressive Agents Behavior, Animal Cyprinodontiformes Female Fluoxetine Swimming Toxicity Tests Water Pollutants, Chemical |
| description |
Chemical pollution from pharmaceuticals is increasingly recognized as a major hazard to the aquatic biota. Among the wide variety of pharmaceuticals, fluoxetine (FLX) is one of the most widely prescribed antidepressants, and therefore, it is frequently identified in the aquatic environment. As FLX is designed to alter human behaviour and many physiological pathways are conserved across vertebrates, this drug may affect the behaviour of fish living in FLX-polluted environments. Here, we exposed groups of female mosquitofish Gambusia holbrooki to waterborne FLX for 14 days, under semi-static conditions with daily renewal of test solutions. Following exposure, we conducted a set of behavioural assays in individual fish, aimed at assessing the effects of FLX on their locomotor activity and behavioural responses. We found that FLX impaired swimming behaviour at high concentrations (25 μg/L and 50 μg/L) but not at low concentrations close to environmental levels (1 μg/L and 5 μg/L). When swimming activity was assessed 5 min after transfer of the focal fish to the testing tank, 50 μg/L FLX was the only concentration showing significant effects. However, when the same trials were performed 24 h later, 25 μg/L FLX turned out to be an effect concentration in addition to 50 μg/L. Interestingly, these concentrations would elicit fish plasma concentrations comprised within the range of human therapeutic doses. When subjected to a light/dark preference test, fish showed tendency to remain less time in the dark area at high FLX concentrations, thus suggesting an anti-anxiety response. Shoaling behaviour was not affected by FLX exposure. Our study contributes to the growing body of literature evaluating the effects of FLX on animal behaviour. Regarding the experimental design used in behavioural testing, our findings suggest that focal fish should be subjected to long habituation periods, namely of at least a few hours, in order to better assess the effects of drug exposure. © 2018 Elsevier Inc. |
| title |
Effects of waterborne exposure to the antidepressant fluoxetine on swimming, shoaling and anxiety behaviours of the mosquitofish Gambusia holbrooki |
| title_short |
Effects of waterborne exposure to the antidepressant fluoxetine on swimming, shoaling and anxiety behaviours of the mosquitofish Gambusia holbrooki |
| title_full |
Effects of waterborne exposure to the antidepressant fluoxetine on swimming, shoaling and anxiety behaviours of the mosquitofish Gambusia holbrooki |
| title_fullStr |
Effects of waterborne exposure to the antidepressant fluoxetine on swimming, shoaling and anxiety behaviours of the mosquitofish Gambusia holbrooki |
| title_full_unstemmed |
Effects of waterborne exposure to the antidepressant fluoxetine on swimming, shoaling and anxiety behaviours of the mosquitofish Gambusia holbrooki |
| title_sort |
effects of waterborne exposure to the antidepressant fluoxetine on swimming, shoaling and anxiety behaviours of the mosquitofish gambusia holbrooki |
| publishDate |
2018 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01476513_v163_n_p646_Meijide http://hdl.handle.net/20.500.12110/paper_01476513_v163_n_p646_Meijide |
| _version_ |
1768544634773962752 |