Cytogenetic damage in peripheral blood cultures of Chaetophractus villosus exposed in vivo to a glyphosate formulation (Roundup)
Different concentrations of a glyphosate formulation, Roundup® Full II (66.2% glyphosate) were tested in culture peripheral blood of armadillo Chaetophractus villosus with cytogenetic biomarkers like mitotic index (MI), chromosomal aberrations (CA), sister chromatid exchange (SCE) and cell prolifera...
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2018
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01476513_v157_n_p121_Rossi http://hdl.handle.net/20.500.12110/paper_01476513_v157_n_p121_Rossi |
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paper:paper_01476513_v157_n_p121_Rossi2023-06-08T15:12:53Z Cytogenetic damage in peripheral blood cultures of Chaetophractus villosus exposed in vivo to a glyphosate formulation (Roundup) Chromosomal aberrations Genotoxicity Replication index Roundup Sister chromatid exchange glyphosate glycine glyphosate mutagenic agent biomarker blood chromosome cytogenetics genotoxicity glyphosate mammal adult animal cell animal experiment armadillo Article cell proliferation Chaetophractus villosus chromosome aberration chromosome breakage concentration (parameters) controlled study cytogenetics female genotoxicity in vivo study long term exposure lymphocyte culture male mitosis index nonhuman sister chromatid exchange analogs and derivatives animal blood chemically induced chromosome analysis drug effect human lymphocyte Animalia Armadillo Chaetophractus villosus Mammalia Animals Armadillos Cell Proliferation Chromosome Aberrations Cytogenetic Analysis Female Glycine Humans Lymphocytes Male Mitotic Index Mutagens Sister Chromatid Exchange Different concentrations of a glyphosate formulation, Roundup® Full II (66.2% glyphosate) were tested in culture peripheral blood of armadillo Chaetophractus villosus with cytogenetic biomarkers like mitotic index (MI), chromosomal aberrations (CA), sister chromatid exchange (SCE) and cell proliferation kinetics (CPK) by means of replication index. Adults animals of both sexes were exposed to RU at four concentrations ranging from 0.026 mL RU solution to 0.379 mL RU daily in oral treatment with the same volume (0.2 mL) during 7 days. We analyzed the induced damage at different times considering T0 as control value, one (T1), seven (T7) and 30 days (T30). One day after, only the higher concentration shows MI significant differences (p < 0.05), at T7 the frequency increases and at T30 it decreases reaching T0 values. The analysis of CA frequencies shows that only 0.106 mL RU/day exhibit significant differences vs T0 values. A great variability is expressed in the values of standard deviation (SD) and in the wide confidence intervals of the media. One day after treatments (T1) all four concentrations shows significant differences in SCE vs T0 values. Replication Index (RI) does not show significant differences. The dose-response behavior was not observed in either CA or SCE. The consistency of the findings obtained with the same biomarkers in vitro support the idea of expanding studies in order to characterize the risk doses for these mammals. © 2018 Elsevier Inc. 2018 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01476513_v157_n_p121_Rossi http://hdl.handle.net/20.500.12110/paper_01476513_v157_n_p121_Rossi |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Chromosomal aberrations Genotoxicity Replication index Roundup Sister chromatid exchange glyphosate glycine glyphosate mutagenic agent biomarker blood chromosome cytogenetics genotoxicity glyphosate mammal adult animal cell animal experiment armadillo Article cell proliferation Chaetophractus villosus chromosome aberration chromosome breakage concentration (parameters) controlled study cytogenetics female genotoxicity in vivo study long term exposure lymphocyte culture male mitosis index nonhuman sister chromatid exchange analogs and derivatives animal blood chemically induced chromosome analysis drug effect human lymphocyte Animalia Armadillo Chaetophractus villosus Mammalia Animals Armadillos Cell Proliferation Chromosome Aberrations Cytogenetic Analysis Female Glycine Humans Lymphocytes Male Mitotic Index Mutagens Sister Chromatid Exchange |
| spellingShingle |
Chromosomal aberrations Genotoxicity Replication index Roundup Sister chromatid exchange glyphosate glycine glyphosate mutagenic agent biomarker blood chromosome cytogenetics genotoxicity glyphosate mammal adult animal cell animal experiment armadillo Article cell proliferation Chaetophractus villosus chromosome aberration chromosome breakage concentration (parameters) controlled study cytogenetics female genotoxicity in vivo study long term exposure lymphocyte culture male mitosis index nonhuman sister chromatid exchange analogs and derivatives animal blood chemically induced chromosome analysis drug effect human lymphocyte Animalia Armadillo Chaetophractus villosus Mammalia Animals Armadillos Cell Proliferation Chromosome Aberrations Cytogenetic Analysis Female Glycine Humans Lymphocytes Male Mitotic Index Mutagens Sister Chromatid Exchange Cytogenetic damage in peripheral blood cultures of Chaetophractus villosus exposed in vivo to a glyphosate formulation (Roundup) |
| topic_facet |
Chromosomal aberrations Genotoxicity Replication index Roundup Sister chromatid exchange glyphosate glycine glyphosate mutagenic agent biomarker blood chromosome cytogenetics genotoxicity glyphosate mammal adult animal cell animal experiment armadillo Article cell proliferation Chaetophractus villosus chromosome aberration chromosome breakage concentration (parameters) controlled study cytogenetics female genotoxicity in vivo study long term exposure lymphocyte culture male mitosis index nonhuman sister chromatid exchange analogs and derivatives animal blood chemically induced chromosome analysis drug effect human lymphocyte Animalia Armadillo Chaetophractus villosus Mammalia Animals Armadillos Cell Proliferation Chromosome Aberrations Cytogenetic Analysis Female Glycine Humans Lymphocytes Male Mitotic Index Mutagens Sister Chromatid Exchange |
| description |
Different concentrations of a glyphosate formulation, Roundup® Full II (66.2% glyphosate) were tested in culture peripheral blood of armadillo Chaetophractus villosus with cytogenetic biomarkers like mitotic index (MI), chromosomal aberrations (CA), sister chromatid exchange (SCE) and cell proliferation kinetics (CPK) by means of replication index. Adults animals of both sexes were exposed to RU at four concentrations ranging from 0.026 mL RU solution to 0.379 mL RU daily in oral treatment with the same volume (0.2 mL) during 7 days. We analyzed the induced damage at different times considering T0 as control value, one (T1), seven (T7) and 30 days (T30). One day after, only the higher concentration shows MI significant differences (p < 0.05), at T7 the frequency increases and at T30 it decreases reaching T0 values. The analysis of CA frequencies shows that only 0.106 mL RU/day exhibit significant differences vs T0 values. A great variability is expressed in the values of standard deviation (SD) and in the wide confidence intervals of the media. One day after treatments (T1) all four concentrations shows significant differences in SCE vs T0 values. Replication Index (RI) does not show significant differences. The dose-response behavior was not observed in either CA or SCE. The consistency of the findings obtained with the same biomarkers in vitro support the idea of expanding studies in order to characterize the risk doses for these mammals. © 2018 Elsevier Inc. |
| title |
Cytogenetic damage in peripheral blood cultures of Chaetophractus villosus exposed in vivo to a glyphosate formulation (Roundup) |
| title_short |
Cytogenetic damage in peripheral blood cultures of Chaetophractus villosus exposed in vivo to a glyphosate formulation (Roundup) |
| title_full |
Cytogenetic damage in peripheral blood cultures of Chaetophractus villosus exposed in vivo to a glyphosate formulation (Roundup) |
| title_fullStr |
Cytogenetic damage in peripheral blood cultures of Chaetophractus villosus exposed in vivo to a glyphosate formulation (Roundup) |
| title_full_unstemmed |
Cytogenetic damage in peripheral blood cultures of Chaetophractus villosus exposed in vivo to a glyphosate formulation (Roundup) |
| title_sort |
cytogenetic damage in peripheral blood cultures of chaetophractus villosus exposed in vivo to a glyphosate formulation (roundup) |
| publishDate |
2018 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01476513_v157_n_p121_Rossi http://hdl.handle.net/20.500.12110/paper_01476513_v157_n_p121_Rossi |
| _version_ |
1768543939791421440 |