Oxidative effects and toxin bioaccumulation after dietary microcystin intoxication in the hepatopancreas of the crab Neohelice (Chasmagnathus) granulata

We studied the accumulation and depuration of microcystin-LR (MCLR) in the hepatopancreas of the crab Neohelice granulata fed twice weekly with either non toxic or MCLR-producing Microcystis aeruginosa (strain NPDC1 or NPJB, respectively) during seven weeks. We also analyzed MCLR effects on the oxid...

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Autores principales:	Sabatini, Sebastián Eduardo, Luquet, Carlos Marcelo
Publicado:	2015
Materias:	ELISA Estuarine crab Hepatopancreas Microcystin depuration Toxin bioaccumulation glutathione glutathione transferase microcystin LR superoxide dismutase thiobarbituric acid reactive substance antioxidant enzyme inhibitor microcystin water pollutant bioaccumulation crab detoxification diet ecotoxicology estuarine ecosystem excretion phytotoxicity toxin adult animal experiment animal tissue antioxidant activity Article bacterial strain clinical trial (topic) controlled study enzyme activity hepatopancreas host resistance long term exposure male Microcystis aeruginosa Neohelice granulata nonhuman oxidative stress toxin analysis xenobiotic metabolism animal Brachyura drug effects lipid peroxidation metabolism Microcystis pharmacokinetics physiology toxicity Chasmagnathus Decapoda (Crustacea) Animals Antioxidants Enzyme Inhibitors Glutathione Glutathione Transferase Lipid Peroxidation Microcystins Oxidative Stress Superoxide Dismutase Water Pollutants, Chemical
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01476513_v120_n_p136_Sabatini http://hdl.handle.net/20.500.12110/paper_01476513_v120_n_p136_Sabatini
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	paper:paper_01476513_v120_n_p136_Sabatini2023-06-08T15:12:51Z Oxidative effects and toxin bioaccumulation after dietary microcystin intoxication in the hepatopancreas of the crab Neohelice (Chasmagnathus) granulata Sabatini, Sebastián Eduardo Luquet, Carlos Marcelo ELISA Estuarine crab Hepatopancreas Microcystin depuration Toxin bioaccumulation glutathione glutathione transferase microcystin LR superoxide dismutase thiobarbituric acid reactive substance antioxidant enzyme inhibitor glutathione glutathione transferase microcystin superoxide dismutase water pollutant antioxidant bioaccumulation crab detoxification diet ecotoxicology estuarine ecosystem excretion phytotoxicity toxin adult animal experiment animal tissue antioxidant activity Article bacterial strain bioaccumulation clinical trial (topic) controlled study crab detoxification enzyme activity excretion hepatopancreas host resistance long term exposure male Microcystis aeruginosa Neohelice granulata nonhuman oxidative stress toxin analysis xenobiotic metabolism animal Brachyura drug effects hepatopancreas lipid peroxidation metabolism Microcystis oxidative stress pharmacokinetics physiology toxicity water pollutant Chasmagnathus Decapoda (Crustacea) Microcystis aeruginosa Animals Antioxidants Brachyura Enzyme Inhibitors Glutathione Glutathione Transferase Hepatopancreas Lipid Peroxidation Microcystins Microcystis Oxidative Stress Superoxide Dismutase Water Pollutants, Chemical We studied the accumulation and depuration of microcystin-LR (MCLR) in the hepatopancreas of the crab Neohelice granulata fed twice weekly with either non toxic or MCLR-producing Microcystis aeruginosa (strain NPDC1 or NPJB, respectively) during seven weeks. We also analyzed MCLR effects on the oxidative stress- and detoxification-related variables, superoxide dismutase and glutathione-S-transferase activities, and the levels of reduced glutathione and lipid peroxidation (as thiobarbituric acid reactive substances, TBARS).Hepatopancreas MCLR content slightly increased during the first three weeks, up to 8.81 ±1.84ng g-1 wet tissue mass (WTM) and then started to decrease to a minimum of 1.57 ±0.74ng g-1 WTM at the seventh week (p <0.05 with respect to that in the first week). TBARS levels were about 55% higher in treated than in control N. granulata (p <0.001 and p <0.05) during the first three weeks of the experimental period. GSH content became 50% lower than in control individuals (p <0.01) during weeks 6 and 7. SOD activity was increased by about 2-fold (p <0.05 or p<0.001) from week 3 to 7 in treated crabs with respect to control ones, while GST activity was about 70% higher in treated than in control crabs from week 4 to week 7 (p <0.05).Our data suggest that in the hepatopancreas of N. granulata MCLR accumulation and oxidative damage are limited and reversed by detoxification-excretion and antioxidant mechanisms. The activation of these defensive mechanisms becomes evident at 3-4 weeks after the start of the intoxication. © 2015 Elsevier Inc. Fil:Sabatini, S.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Luquet, C.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2015 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01476513_v120_n_p136_Sabatini http://hdl.handle.net/20.500.12110/paper_01476513_v120_n_p136_Sabatini
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	ELISA Estuarine crab Hepatopancreas Microcystin depuration Toxin bioaccumulation glutathione glutathione transferase microcystin LR superoxide dismutase thiobarbituric acid reactive substance antioxidant enzyme inhibitor glutathione glutathione transferase microcystin superoxide dismutase water pollutant antioxidant bioaccumulation crab detoxification diet ecotoxicology estuarine ecosystem excretion phytotoxicity toxin adult animal experiment animal tissue antioxidant activity Article bacterial strain bioaccumulation clinical trial (topic) controlled study crab detoxification enzyme activity excretion hepatopancreas host resistance long term exposure male Microcystis aeruginosa Neohelice granulata nonhuman oxidative stress toxin analysis xenobiotic metabolism animal Brachyura drug effects hepatopancreas lipid peroxidation metabolism Microcystis oxidative stress pharmacokinetics physiology toxicity water pollutant Chasmagnathus Decapoda (Crustacea) Microcystis aeruginosa Animals Antioxidants Brachyura Enzyme Inhibitors Glutathione Glutathione Transferase Hepatopancreas Lipid Peroxidation Microcystins Microcystis Oxidative Stress Superoxide Dismutase Water Pollutants, Chemical
spellingShingle	ELISA Estuarine crab Hepatopancreas Microcystin depuration Toxin bioaccumulation glutathione glutathione transferase microcystin LR superoxide dismutase thiobarbituric acid reactive substance antioxidant enzyme inhibitor glutathione glutathione transferase microcystin superoxide dismutase water pollutant antioxidant bioaccumulation crab detoxification diet ecotoxicology estuarine ecosystem excretion phytotoxicity toxin adult animal experiment animal tissue antioxidant activity Article bacterial strain bioaccumulation clinical trial (topic) controlled study crab detoxification enzyme activity excretion hepatopancreas host resistance long term exposure male Microcystis aeruginosa Neohelice granulata nonhuman oxidative stress toxin analysis xenobiotic metabolism animal Brachyura drug effects hepatopancreas lipid peroxidation metabolism Microcystis oxidative stress pharmacokinetics physiology toxicity water pollutant Chasmagnathus Decapoda (Crustacea) Microcystis aeruginosa Animals Antioxidants Brachyura Enzyme Inhibitors Glutathione Glutathione Transferase Hepatopancreas Lipid Peroxidation Microcystins Microcystis Oxidative Stress Superoxide Dismutase Water Pollutants, Chemical Sabatini, Sebastián Eduardo Luquet, Carlos Marcelo Oxidative effects and toxin bioaccumulation after dietary microcystin intoxication in the hepatopancreas of the crab Neohelice (Chasmagnathus) granulata
topic_facet	ELISA Estuarine crab Hepatopancreas Microcystin depuration Toxin bioaccumulation glutathione glutathione transferase microcystin LR superoxide dismutase thiobarbituric acid reactive substance antioxidant enzyme inhibitor glutathione glutathione transferase microcystin superoxide dismutase water pollutant antioxidant bioaccumulation crab detoxification diet ecotoxicology estuarine ecosystem excretion phytotoxicity toxin adult animal experiment animal tissue antioxidant activity Article bacterial strain bioaccumulation clinical trial (topic) controlled study crab detoxification enzyme activity excretion hepatopancreas host resistance long term exposure male Microcystis aeruginosa Neohelice granulata nonhuman oxidative stress toxin analysis xenobiotic metabolism animal Brachyura drug effects hepatopancreas lipid peroxidation metabolism Microcystis oxidative stress pharmacokinetics physiology toxicity water pollutant Chasmagnathus Decapoda (Crustacea) Microcystis aeruginosa Animals Antioxidants Brachyura Enzyme Inhibitors Glutathione Glutathione Transferase Hepatopancreas Lipid Peroxidation Microcystins Microcystis Oxidative Stress Superoxide Dismutase Water Pollutants, Chemical
description	We studied the accumulation and depuration of microcystin-LR (MCLR) in the hepatopancreas of the crab Neohelice granulata fed twice weekly with either non toxic or MCLR-producing Microcystis aeruginosa (strain NPDC1 or NPJB, respectively) during seven weeks. We also analyzed MCLR effects on the oxidative stress- and detoxification-related variables, superoxide dismutase and glutathione-S-transferase activities, and the levels of reduced glutathione and lipid peroxidation (as thiobarbituric acid reactive substances, TBARS).Hepatopancreas MCLR content slightly increased during the first three weeks, up to 8.81 ±1.84ng g-1 wet tissue mass (WTM) and then started to decrease to a minimum of 1.57 ±0.74ng g-1 WTM at the seventh week (p <0.05 with respect to that in the first week). TBARS levels were about 55% higher in treated than in control N. granulata (p <0.001 and p <0.05) during the first three weeks of the experimental period. GSH content became 50% lower than in control individuals (p <0.01) during weeks 6 and 7. SOD activity was increased by about 2-fold (p <0.05 or p<0.001) from week 3 to 7 in treated crabs with respect to control ones, while GST activity was about 70% higher in treated than in control crabs from week 4 to week 7 (p <0.05).Our data suggest that in the hepatopancreas of N. granulata MCLR accumulation and oxidative damage are limited and reversed by detoxification-excretion and antioxidant mechanisms. The activation of these defensive mechanisms becomes evident at 3-4 weeks after the start of the intoxication. © 2015 Elsevier Inc.
author	Sabatini, Sebastián Eduardo Luquet, Carlos Marcelo
author_facet	Sabatini, Sebastián Eduardo Luquet, Carlos Marcelo
author_sort	Sabatini, Sebastián Eduardo
title	Oxidative effects and toxin bioaccumulation after dietary microcystin intoxication in the hepatopancreas of the crab Neohelice (Chasmagnathus) granulata
title_short	Oxidative effects and toxin bioaccumulation after dietary microcystin intoxication in the hepatopancreas of the crab Neohelice (Chasmagnathus) granulata
title_full	Oxidative effects and toxin bioaccumulation after dietary microcystin intoxication in the hepatopancreas of the crab Neohelice (Chasmagnathus) granulata
title_fullStr	Oxidative effects and toxin bioaccumulation after dietary microcystin intoxication in the hepatopancreas of the crab Neohelice (Chasmagnathus) granulata
title_full_unstemmed	Oxidative effects and toxin bioaccumulation after dietary microcystin intoxication in the hepatopancreas of the crab Neohelice (Chasmagnathus) granulata
title_sort	oxidative effects and toxin bioaccumulation after dietary microcystin intoxication in the hepatopancreas of the crab neohelice (chasmagnathus) granulata
publishDate	2015
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01476513_v120_n_p136_Sabatini http://hdl.handle.net/20.500.12110/paper_01476513_v120_n_p136_Sabatini
work_keys_str_mv	AT sabatinisebastianeduardo oxidativeeffectsandtoxinbioaccumulationafterdietarymicrocystinintoxicationinthehepatopancreasofthecrabneohelicechasmagnathusgranulata AT luquetcarlosmarcelo oxidativeeffectsandtoxinbioaccumulationafterdietarymicrocystinintoxicationinthehepatopancreasofthecrabneohelicechasmagnathusgranulata
_version_	1768543987280379904

Oxidative effects and toxin bioaccumulation after dietary microcystin intoxication in the hepatopancreas of the crab Neohelice (Chasmagnathus) granulata

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