Endosulfan affects GnRH cells in sexually differentiated juveniles of the perciform Cichlasoma dimerus

Endosulfan (ES) is an organochlorine pesticide widely used in agriculture despite its high toxicity towards non-target organisms such as fish. It has been demonstrated that ES can cause negative effects on aquatic animals, including disruption of hormonal systems. However, the alterations produced b...

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Autores principales:	Piazza, Yanina Grisel, Pandolfi, Matías, Da Cuña, Rodrigo Hernán, Genovese, Griselda, Lo Nostro, Fabiana Laura
Publicado:	2015
Materias:	Cichlids GnRH Gonadotrops Organochlorine pesticides Reproductive axis endocrine disruptor endosulfan gonadorelin chlorinated hydrocarbon fresh water insecticide progonadoliberin I protein precursor water pollutant ecotoxicology hormone juvenile morphometry perciform pollution effect pollution exposure reproductive behavior sexual maturity adult animal cell animal experiment animal tissue Article biological model body weight brain nerve cell cell nucleus cell structure Cichlasoma dimerus cichlid controlled study cytoplasm development exposure female gonad juvenile animal larva male morphometrics nonhuman nutritional status Perciformes reproductive fitness reproductive toxicity sex differentiation sex ratio analysis of variance animal drug effects metabolism nerve cell physiology toxicity Animalia Cichlasoma Cichlidae Analysis of Variance Animals Endocrine Disruptors Endosulfan Fresh Water Gonadotropin-Releasing Hormone Gonads Hydrocarbons, Chlorinated Insecticides Larva Neurons Protein Precursors Sex Differentiation Water Pollutants, Chemical
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01476513_v116_n_p150_Piazza http://hdl.handle.net/20.500.12110/paper_01476513_v116_n_p150_Piazza
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_01476513_v116_n_p150_Piazza
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spelling	paper:paper_01476513_v116_n_p150_Piazza2023-06-08T15:12:50Z Endosulfan affects GnRH cells in sexually differentiated juveniles of the perciform Cichlasoma dimerus Piazza, Yanina Grisel Pandolfi, Matías Da Cuña, Rodrigo Hernán Genovese, Griselda Lo Nostro, Fabiana Laura Cichlids GnRH Gonadotrops Organochlorine pesticides Reproductive axis endocrine disruptor endosulfan gonadorelin chlorinated hydrocarbon endocrine disruptor endosulfan fresh water gonadorelin insecticide progonadoliberin I protein precursor water pollutant ecotoxicology endocrine disruptor endosulfan hormone juvenile morphometry perciform pollution effect pollution exposure reproductive behavior sexual maturity adult animal cell animal experiment animal tissue Article biological model body weight brain nerve cell cell nucleus cell structure Cichlasoma dimerus cichlid controlled study cytoplasm development exposure female gonad juvenile animal larva male morphometrics nonhuman nutritional status Perciformes reproductive fitness reproductive toxicity sex differentiation sex ratio analysis of variance animal cichlid drug effects metabolism nerve cell physiology toxicity water pollutant Animalia Cichlasoma Cichlidae Perciformes Analysis of Variance Animals Cichlids Endocrine Disruptors Endosulfan Fresh Water Gonadotropin-Releasing Hormone Gonads Hydrocarbons, Chlorinated Insecticides Larva Neurons Protein Precursors Sex Differentiation Water Pollutants, Chemical Endosulfan (ES) is an organochlorine pesticide widely used in agriculture despite its high toxicity towards non-target organisms such as fish. It has been demonstrated that ES can cause negative effects on aquatic animals, including disruption of hormonal systems. However, the alterations produced by this pesticide on the reproductive axis of fish prior to sexual maturity, as well as possible modes of action have hardly been studied. This study aimed at assessing the effect of waterborne exposure to the pesticide ES on the reproductive axis during sexual differentiation of juveniles of the South American freshwater cichlid fish Cichlasoma dimerus. No mortality was observed due to ES subchronic exposure (90 days post-fertilization). Exposure to ES did not affect body weight nor morphometric parameters, indicating that larvae nutritional state was not affected. Timing of sexual differentiation, gonadal morphology and sex ratio were likewise not altered by ES. However, ES acted as an endocrine disrupting chemical in this species as the morphometry of gonadotropin-releasing hormones (GnRH) producing cells was altered. Exposure to ES altered nuclear area, cell area and nucleus/cytoplasm ratio of GnRH II neurons, and cell and nuclear area and diameter of GnRH III neurons. Interestingly, in our previous study, exposure before sex differentiation (30 day exposure) caused no alteration to GnRH II and III, and did alter GnRH I and FSH cells. These alterations could lead to changes in circulating hormone levels, especially when fish are exposed for prolonged periods, ultimately impairing reproductive fitness. C. dimerus juveniles can be an interesting biological model to perform toxicological studies with the intent to assess early disruption endpoints in the reproductive axis during development. © 2015 Elsevier Inc. Fil:Piazza, Y. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pandolfi, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Da Cuña, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Genovese, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lo Nostro, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2015 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01476513_v116_n_p150_Piazza http://hdl.handle.net/20.500.12110/paper_01476513_v116_n_p150_Piazza
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Cichlids GnRH Gonadotrops Organochlorine pesticides Reproductive axis endocrine disruptor endosulfan gonadorelin chlorinated hydrocarbon endocrine disruptor endosulfan fresh water gonadorelin insecticide progonadoliberin I protein precursor water pollutant ecotoxicology endocrine disruptor endosulfan hormone juvenile morphometry perciform pollution effect pollution exposure reproductive behavior sexual maturity adult animal cell animal experiment animal tissue Article biological model body weight brain nerve cell cell nucleus cell structure Cichlasoma dimerus cichlid controlled study cytoplasm development exposure female gonad juvenile animal larva male morphometrics nonhuman nutritional status Perciformes reproductive fitness reproductive toxicity sex differentiation sex ratio analysis of variance animal cichlid drug effects metabolism nerve cell physiology toxicity water pollutant Animalia Cichlasoma Cichlidae Perciformes Analysis of Variance Animals Cichlids Endocrine Disruptors Endosulfan Fresh Water Gonadotropin-Releasing Hormone Gonads Hydrocarbons, Chlorinated Insecticides Larva Neurons Protein Precursors Sex Differentiation Water Pollutants, Chemical
spellingShingle	Cichlids GnRH Gonadotrops Organochlorine pesticides Reproductive axis endocrine disruptor endosulfan gonadorelin chlorinated hydrocarbon endocrine disruptor endosulfan fresh water gonadorelin insecticide progonadoliberin I protein precursor water pollutant ecotoxicology endocrine disruptor endosulfan hormone juvenile morphometry perciform pollution effect pollution exposure reproductive behavior sexual maturity adult animal cell animal experiment animal tissue Article biological model body weight brain nerve cell cell nucleus cell structure Cichlasoma dimerus cichlid controlled study cytoplasm development exposure female gonad juvenile animal larva male morphometrics nonhuman nutritional status Perciformes reproductive fitness reproductive toxicity sex differentiation sex ratio analysis of variance animal cichlid drug effects metabolism nerve cell physiology toxicity water pollutant Animalia Cichlasoma Cichlidae Perciformes Analysis of Variance Animals Cichlids Endocrine Disruptors Endosulfan Fresh Water Gonadotropin-Releasing Hormone Gonads Hydrocarbons, Chlorinated Insecticides Larva Neurons Protein Precursors Sex Differentiation Water Pollutants, Chemical Piazza, Yanina Grisel Pandolfi, Matías Da Cuña, Rodrigo Hernán Genovese, Griselda Lo Nostro, Fabiana Laura Endosulfan affects GnRH cells in sexually differentiated juveniles of the perciform Cichlasoma dimerus
topic_facet	Cichlids GnRH Gonadotrops Organochlorine pesticides Reproductive axis endocrine disruptor endosulfan gonadorelin chlorinated hydrocarbon endocrine disruptor endosulfan fresh water gonadorelin insecticide progonadoliberin I protein precursor water pollutant ecotoxicology endocrine disruptor endosulfan hormone juvenile morphometry perciform pollution effect pollution exposure reproductive behavior sexual maturity adult animal cell animal experiment animal tissue Article biological model body weight brain nerve cell cell nucleus cell structure Cichlasoma dimerus cichlid controlled study cytoplasm development exposure female gonad juvenile animal larva male morphometrics nonhuman nutritional status Perciformes reproductive fitness reproductive toxicity sex differentiation sex ratio analysis of variance animal cichlid drug effects metabolism nerve cell physiology toxicity water pollutant Animalia Cichlasoma Cichlidae Perciformes Analysis of Variance Animals Cichlids Endocrine Disruptors Endosulfan Fresh Water Gonadotropin-Releasing Hormone Gonads Hydrocarbons, Chlorinated Insecticides Larva Neurons Protein Precursors Sex Differentiation Water Pollutants, Chemical
description	Endosulfan (ES) is an organochlorine pesticide widely used in agriculture despite its high toxicity towards non-target organisms such as fish. It has been demonstrated that ES can cause negative effects on aquatic animals, including disruption of hormonal systems. However, the alterations produced by this pesticide on the reproductive axis of fish prior to sexual maturity, as well as possible modes of action have hardly been studied. This study aimed at assessing the effect of waterborne exposure to the pesticide ES on the reproductive axis during sexual differentiation of juveniles of the South American freshwater cichlid fish Cichlasoma dimerus. No mortality was observed due to ES subchronic exposure (90 days post-fertilization). Exposure to ES did not affect body weight nor morphometric parameters, indicating that larvae nutritional state was not affected. Timing of sexual differentiation, gonadal morphology and sex ratio were likewise not altered by ES. However, ES acted as an endocrine disrupting chemical in this species as the morphometry of gonadotropin-releasing hormones (GnRH) producing cells was altered. Exposure to ES altered nuclear area, cell area and nucleus/cytoplasm ratio of GnRH II neurons, and cell and nuclear area and diameter of GnRH III neurons. Interestingly, in our previous study, exposure before sex differentiation (30 day exposure) caused no alteration to GnRH II and III, and did alter GnRH I and FSH cells. These alterations could lead to changes in circulating hormone levels, especially when fish are exposed for prolonged periods, ultimately impairing reproductive fitness. C. dimerus juveniles can be an interesting biological model to perform toxicological studies with the intent to assess early disruption endpoints in the reproductive axis during development. © 2015 Elsevier Inc.
author	Piazza, Yanina Grisel Pandolfi, Matías Da Cuña, Rodrigo Hernán Genovese, Griselda Lo Nostro, Fabiana Laura
author_facet	Piazza, Yanina Grisel Pandolfi, Matías Da Cuña, Rodrigo Hernán Genovese, Griselda Lo Nostro, Fabiana Laura
author_sort	Piazza, Yanina Grisel
title	Endosulfan affects GnRH cells in sexually differentiated juveniles of the perciform Cichlasoma dimerus
title_short	Endosulfan affects GnRH cells in sexually differentiated juveniles of the perciform Cichlasoma dimerus
title_full	Endosulfan affects GnRH cells in sexually differentiated juveniles of the perciform Cichlasoma dimerus
title_fullStr	Endosulfan affects GnRH cells in sexually differentiated juveniles of the perciform Cichlasoma dimerus
title_full_unstemmed	Endosulfan affects GnRH cells in sexually differentiated juveniles of the perciform Cichlasoma dimerus
title_sort	endosulfan affects gnrh cells in sexually differentiated juveniles of the perciform cichlasoma dimerus
publishDate	2015
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01476513_v116_n_p150_Piazza http://hdl.handle.net/20.500.12110/paper_01476513_v116_n_p150_Piazza
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Endosulfan affects GnRH cells in sexually differentiated juveniles of the perciform Cichlasoma dimerus

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