Subcellular energy balance of Odontesthes bonariensis exposed to a glyphosate-based herbicide
Water pollution by agrochemicals is currently one of the most critical problems for the conservation of aquatic ecosystems. Glyphosate [N-(phosphonomethyl) glycine); PMG] is the main broad-spectrum post emergence herbicide used for the control of a wide range of pests in soybean crops. Adenylate ene...
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2015
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01476513_v114_n_p157_MenendezHelman http://hdl.handle.net/20.500.12110/paper_01476513_v114_n_p157_MenendezHelman |
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paper:paper_01476513_v114_n_p157_MenendezHelman2023-06-08T15:12:50Z Subcellular energy balance of Odontesthes bonariensis exposed to a glyphosate-based herbicide Menéndez Helman, Renata Julia Miranda, Leandro Andrés Adenylate energy charge (AEC) Based herbicide Biomarkers Glyphosate Odontesthes bonariensis Phosphagens adenosine diphosphate adenosine phosphate adenosine triphosphate creatine phosphate glyphosate adenine nucleotide biological marker glycine glyphosate herbicide water pollutant agrochemical biomarker fish glyphosate pollution exposure sublethal effect adsorption kinetics animal experiment animal tissue Article brain level cell energy concentration (parameters) controlled study degradation kinetics energy balance environmental exposure female fish high performance liquid chromatography liver level long term exposure male muscle level muscle tissue nonhuman Odontesthes bonariensis water pollution analogs and derivatives animal brain dose response drug effects energy metabolism liver metabolism muscle Smegmamorpha toxicity water pollutant Animalia Glycine max Odontesthes bonariensis Adenine Nucleotides Animals Biological Markers Brain Dose-Response Relationship, Drug Energy Metabolism Glycine Herbicides Liver Muscles Phosphocreatine Smegmamorpha Water Pollutants, Chemical Water pollution by agrochemicals is currently one of the most critical problems for the conservation of aquatic ecosystems. Glyphosate [N-(phosphonomethyl) glycine); PMG] is the main broad-spectrum post emergence herbicide used for the control of a wide range of pests in soybean crops. Adenylate energy charge (AEC) reflects the energy balance of the cells, a measure of the energy available from the adenylate pool: adenosine triphosphate (ATP), adenosine diphosphate (ADP) and adenosine monophosphate (AMP). Background adenylates, phosphagens and the AEC index of two year old Odontesthes bonariensis were determined in some tissues by HPLC, and the impact on subcellular energy balance of sublethal glyphosate-based herbicide exposure was analyzed. The doses used were 0 (control tank), 1 or 10mg PMGL-1, trials were carried out during 15 days. AEC values in brain, liver and muscle from control fish were 0.37±0.02, 0.49±0.05 and 0.56±0.03, respectively (means±SEM). While brain ATP concentrations were undetectable (hence low values of AEC), the muscle tissue showed the highest concentrations of the more energetic molecules: 0.18μmole ATPg-1 and 8μmole phosphocreatineg-1 (PCrg-1). In the brain, no significant changes were detected in exposed fish compared to controls. Instead, in both the liver and muscle of animals exposed to the highest concentration of the herbicide, significant changes in the AEC (reduction of 26% and 15%, p<0.05) with respect to the control group were determined. Chronic exposure (15 days) of Odontesthes bonariensis to 1 and 10mgL-1 of formulated glyphosate did not affect brain AEC. However, the highest concentration of the herbicide produced a significant decrease in liver and muscle AEC manifesting adverse sublethal effects on the energy metabolism. These results suggest the usefulness of AEC as a biomarker of fish glyphosate exposure. © 2015 Elsevier Inc. Fil:Menéndez-Helman, R.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Miranda, L.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2015 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01476513_v114_n_p157_MenendezHelman http://hdl.handle.net/20.500.12110/paper_01476513_v114_n_p157_MenendezHelman |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Adenylate energy charge (AEC) Based herbicide Biomarkers Glyphosate Odontesthes bonariensis Phosphagens adenosine diphosphate adenosine phosphate adenosine triphosphate creatine phosphate glyphosate adenine nucleotide biological marker glycine glyphosate herbicide water pollutant agrochemical biomarker fish glyphosate pollution exposure sublethal effect adsorption kinetics animal experiment animal tissue Article brain level cell energy concentration (parameters) controlled study degradation kinetics energy balance environmental exposure female fish high performance liquid chromatography liver level long term exposure male muscle level muscle tissue nonhuman Odontesthes bonariensis water pollution analogs and derivatives animal brain dose response drug effects energy metabolism liver metabolism muscle Smegmamorpha toxicity water pollutant Animalia Glycine max Odontesthes bonariensis Adenine Nucleotides Animals Biological Markers Brain Dose-Response Relationship, Drug Energy Metabolism Glycine Herbicides Liver Muscles Phosphocreatine Smegmamorpha Water Pollutants, Chemical |
| spellingShingle |
Adenylate energy charge (AEC) Based herbicide Biomarkers Glyphosate Odontesthes bonariensis Phosphagens adenosine diphosphate adenosine phosphate adenosine triphosphate creatine phosphate glyphosate adenine nucleotide biological marker glycine glyphosate herbicide water pollutant agrochemical biomarker fish glyphosate pollution exposure sublethal effect adsorption kinetics animal experiment animal tissue Article brain level cell energy concentration (parameters) controlled study degradation kinetics energy balance environmental exposure female fish high performance liquid chromatography liver level long term exposure male muscle level muscle tissue nonhuman Odontesthes bonariensis water pollution analogs and derivatives animal brain dose response drug effects energy metabolism liver metabolism muscle Smegmamorpha toxicity water pollutant Animalia Glycine max Odontesthes bonariensis Adenine Nucleotides Animals Biological Markers Brain Dose-Response Relationship, Drug Energy Metabolism Glycine Herbicides Liver Muscles Phosphocreatine Smegmamorpha Water Pollutants, Chemical Menéndez Helman, Renata Julia Miranda, Leandro Andrés Subcellular energy balance of Odontesthes bonariensis exposed to a glyphosate-based herbicide |
| topic_facet |
Adenylate energy charge (AEC) Based herbicide Biomarkers Glyphosate Odontesthes bonariensis Phosphagens adenosine diphosphate adenosine phosphate adenosine triphosphate creatine phosphate glyphosate adenine nucleotide biological marker glycine glyphosate herbicide water pollutant agrochemical biomarker fish glyphosate pollution exposure sublethal effect adsorption kinetics animal experiment animal tissue Article brain level cell energy concentration (parameters) controlled study degradation kinetics energy balance environmental exposure female fish high performance liquid chromatography liver level long term exposure male muscle level muscle tissue nonhuman Odontesthes bonariensis water pollution analogs and derivatives animal brain dose response drug effects energy metabolism liver metabolism muscle Smegmamorpha toxicity water pollutant Animalia Glycine max Odontesthes bonariensis Adenine Nucleotides Animals Biological Markers Brain Dose-Response Relationship, Drug Energy Metabolism Glycine Herbicides Liver Muscles Phosphocreatine Smegmamorpha Water Pollutants, Chemical |
| description |
Water pollution by agrochemicals is currently one of the most critical problems for the conservation of aquatic ecosystems. Glyphosate [N-(phosphonomethyl) glycine); PMG] is the main broad-spectrum post emergence herbicide used for the control of a wide range of pests in soybean crops. Adenylate energy charge (AEC) reflects the energy balance of the cells, a measure of the energy available from the adenylate pool: adenosine triphosphate (ATP), adenosine diphosphate (ADP) and adenosine monophosphate (AMP). Background adenylates, phosphagens and the AEC index of two year old Odontesthes bonariensis were determined in some tissues by HPLC, and the impact on subcellular energy balance of sublethal glyphosate-based herbicide exposure was analyzed. The doses used were 0 (control tank), 1 or 10mg PMGL-1, trials were carried out during 15 days. AEC values in brain, liver and muscle from control fish were 0.37±0.02, 0.49±0.05 and 0.56±0.03, respectively (means±SEM). While brain ATP concentrations were undetectable (hence low values of AEC), the muscle tissue showed the highest concentrations of the more energetic molecules: 0.18μmole ATPg-1 and 8μmole phosphocreatineg-1 (PCrg-1). In the brain, no significant changes were detected in exposed fish compared to controls. Instead, in both the liver and muscle of animals exposed to the highest concentration of the herbicide, significant changes in the AEC (reduction of 26% and 15%, p<0.05) with respect to the control group were determined. Chronic exposure (15 days) of Odontesthes bonariensis to 1 and 10mgL-1 of formulated glyphosate did not affect brain AEC. However, the highest concentration of the herbicide produced a significant decrease in liver and muscle AEC manifesting adverse sublethal effects on the energy metabolism. These results suggest the usefulness of AEC as a biomarker of fish glyphosate exposure. © 2015 Elsevier Inc. |
| author |
Menéndez Helman, Renata Julia Miranda, Leandro Andrés |
| author_facet |
Menéndez Helman, Renata Julia Miranda, Leandro Andrés |
| author_sort |
Menéndez Helman, Renata Julia |
| title |
Subcellular energy balance of Odontesthes bonariensis exposed to a glyphosate-based herbicide |
| title_short |
Subcellular energy balance of Odontesthes bonariensis exposed to a glyphosate-based herbicide |
| title_full |
Subcellular energy balance of Odontesthes bonariensis exposed to a glyphosate-based herbicide |
| title_fullStr |
Subcellular energy balance of Odontesthes bonariensis exposed to a glyphosate-based herbicide |
| title_full_unstemmed |
Subcellular energy balance of Odontesthes bonariensis exposed to a glyphosate-based herbicide |
| title_sort |
subcellular energy balance of odontesthes bonariensis exposed to a glyphosate-based herbicide |
| publishDate |
2015 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01476513_v114_n_p157_MenendezHelman http://hdl.handle.net/20.500.12110/paper_01476513_v114_n_p157_MenendezHelman |
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AT menendezhelmanrenatajulia subcellularenergybalanceofodontesthesbonariensisexposedtoaglyphosatebasedherbicide AT mirandaleandroandres subcellularenergybalanceofodontesthesbonariensisexposedtoaglyphosatebasedherbicide |
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