Resistance in cholinesterase activity after an acute and subchronic exposure to azinphos-methyl in the freshwater gastropod Biomphalaria straminea
Organophosphorous and carbamates insecticides are ones of the most popular classes of pesticides used in agriculture. Its success relies on their high acute toxicity and rapid environmental degradation. These insecticides inhibit cholinesterase and cause severe effects on aquatic non-target species,...
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| Publicado: |
2014
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01476513_v109_n_p85_Bianco http://hdl.handle.net/20.500.12110/paper_01476513_v109_n_p85_Bianco |
| Aporte de: |
| id |
paper:paper_01476513_v109_n_p85_Bianco |
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| record_format |
dspace |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Biomarkers Carboxylesterases Cholinesterases Organophosphorous insecticides Proteins 1,5 bis(4 allyldimethylammoniumphenyl)pentan 3 one dibromide acetylthiocholine azinphos methyl butyrylthiocholine carboxylesterase choline derivative cholinesterase cholinesterase inhibitor iso OMPA physostigmine propionylthiocholine unclassified drug azinphos methyl biological marker carboxylesterase cholinesterase insecticide water pollutant acetylthiocholine biological marker butyrylthiocholine choline derivative fresh water propionylthiocholine azinphos methyl insecticide azinphos methyl biomarker biomonitoring carbamate (ester) concentration (composition) enzyme activity gastropod insecticide protein toxicity nontarget organism pollution exposure animal tissue article bioassay Biomphalaria straminea concentration (parameters) controlled study enzyme activity enzyme inhibition enzyme kinetics exposure hydrolysis IC 50 in vitro study nonhuman protein content sensitivity analysis survival tissue homogenate animal Biomphalaria drug effects environmental exposure environmental monitoring enzymology metabolism toxicity water pollutant Article biological monitoring IC50 neurotoxicity protein analysis protein function protein hydrolysis protein metabolism Argentina Patagonia Animals Azinphosmethyl Biological Markers Biomphalaria Carboxylic Ester Hydrolases Cholinesterases Environmental Exposure Environmental Monitoring Insecticides Water Pollutants, Chemical Biomphalaria straminea Gastropoda |
| spellingShingle |
Biomarkers Carboxylesterases Cholinesterases Organophosphorous insecticides Proteins 1,5 bis(4 allyldimethylammoniumphenyl)pentan 3 one dibromide acetylthiocholine azinphos methyl butyrylthiocholine carboxylesterase choline derivative cholinesterase cholinesterase inhibitor iso OMPA physostigmine propionylthiocholine unclassified drug azinphos methyl biological marker carboxylesterase cholinesterase insecticide water pollutant acetylthiocholine biological marker butyrylthiocholine choline derivative fresh water propionylthiocholine azinphos methyl insecticide azinphos methyl biomarker biomonitoring carbamate (ester) concentration (composition) enzyme activity gastropod insecticide protein toxicity nontarget organism pollution exposure animal tissue article bioassay Biomphalaria straminea concentration (parameters) controlled study enzyme activity enzyme inhibition enzyme kinetics exposure hydrolysis IC 50 in vitro study nonhuman protein content sensitivity analysis survival tissue homogenate animal Biomphalaria drug effects environmental exposure environmental monitoring enzymology metabolism toxicity water pollutant Article biological monitoring IC50 neurotoxicity protein analysis protein function protein hydrolysis protein metabolism Argentina Patagonia Animals Azinphosmethyl Biological Markers Biomphalaria Carboxylic Ester Hydrolases Cholinesterases Environmental Exposure Environmental Monitoring Insecticides Water Pollutants, Chemical Biomphalaria straminea Gastropoda Nahabedian, Daniel Eduardo Kristoff, Gisela Resistance in cholinesterase activity after an acute and subchronic exposure to azinphos-methyl in the freshwater gastropod Biomphalaria straminea |
| topic_facet |
Biomarkers Carboxylesterases Cholinesterases Organophosphorous insecticides Proteins 1,5 bis(4 allyldimethylammoniumphenyl)pentan 3 one dibromide acetylthiocholine azinphos methyl butyrylthiocholine carboxylesterase choline derivative cholinesterase cholinesterase inhibitor iso OMPA physostigmine propionylthiocholine unclassified drug azinphos methyl biological marker carboxylesterase cholinesterase insecticide water pollutant acetylthiocholine biological marker butyrylthiocholine choline derivative fresh water propionylthiocholine azinphos methyl insecticide azinphos methyl biomarker biomonitoring carbamate (ester) concentration (composition) enzyme activity gastropod insecticide protein toxicity nontarget organism pollution exposure animal tissue article bioassay Biomphalaria straminea concentration (parameters) controlled study enzyme activity enzyme inhibition enzyme kinetics exposure hydrolysis IC 50 in vitro study nonhuman protein content sensitivity analysis survival tissue homogenate animal Biomphalaria drug effects environmental exposure environmental monitoring enzymology metabolism toxicity water pollutant Article biological monitoring IC50 neurotoxicity protein analysis protein function protein hydrolysis protein metabolism Argentina Patagonia Animals Azinphosmethyl Biological Markers Biomphalaria Carboxylic Ester Hydrolases Cholinesterases Environmental Exposure Environmental Monitoring Insecticides Water Pollutants, Chemical Biomphalaria straminea Gastropoda |
| description |
Organophosphorous and carbamates insecticides are ones of the most popular classes of pesticides used in agriculture. Its success relies on their high acute toxicity and rapid environmental degradation. These insecticides inhibit cholinesterase and cause severe effects on aquatic non-target species, particularly in invertebrates. Since the properties of cholinesterases may differ between species, it is necessary to characterize them before their use as biomarkers. Also organophosphorous and carbamates inhibit carboxylesterases and the use of both enzymes for biomonitoring is suggested. Azinphos-methyl is an organophosphorous insecticide used in several parts of the word. In Argentina, it is the most applied insecticide in fruit production in the north Patagonian region. It was detected with the highest frequency in superficial and groundwater of the region. This work aims to evaluate the sensitivity of B. straminea cholinesterases and carboxylesterases to the OP azinphos-methyl including estimations of 48h NOEC and IC50 of the pesticide and subchronic effects at environmentally relevant concentrations. These will allow us to evaluate the possibility of using cholinesterase and carboxylesterase of B. straminea as sensitive biomarkers. Previously a partial characterization of these enzymes will be performed. As in most invertebrates, acetylthiocholine was the preferred hydrolyzed substrate of B. straminea ChE, followed by propionylthiocholine and being butyrylthiocholine hydrolysis very low. Cholinesterase activity of B. straminea was significantly inhibited by the selective cholinesterases inhibitor (eserine) and by the selective inhibitor of mammalian acethylcholinesterase (BW284c51). In contrast, iso-OMPA, a specific inhibitor of butyrylcholinesterase, did not inhibit cholinesterase activity. These results suggest that cholinesterase activity in total soft tissue of B. straminea corresponds to acethylcholinesterase. Carboxylesterases activity was one order of magnitude higher than cholinesterase. A greater efficiency (Vmax/Km) was obtained using acetylthiocholine and p-nitrophenyl butyrate. Acute exposure to azinphos-methyl did not cause inhibition of cholinesterase activity until 10mgL-1 used. Carboxylesterases towards p-nitrophenyl butyrate was inhibited by azinphos-methyl being the IC502.20±0.75mgL-1 of azinphos-methyl. Subchronic exposure to environmental concentrations of azinphos-methyl (0.02 and 0.2mgL-1) produced a decrease in survival, protein content and carboxylesterases activity despite no inhibition of cholinesterase activity was observed. B. straminea cholinesterase is not a sensible biomarker. On the contrary, carboxylesterases activity was inhibited by azinphos-methyl. Carboxylesterases could be protecting cholinesterase activity and therefore, protecting the organism from neurotoxicity. This work confirms the advantages of measuring cholinesterases and carboxylesterases jointly in aquatic biomonitoring of pesticide contamination. This becomes relevant in order to find more sensitive biomarkers and new strategies to protect non-target aquatic organisms from pesticide contamination. © 2014 Elsevier Inc. |
| author |
Nahabedian, Daniel Eduardo Kristoff, Gisela |
| author_facet |
Nahabedian, Daniel Eduardo Kristoff, Gisela |
| author_sort |
Nahabedian, Daniel Eduardo |
| title |
Resistance in cholinesterase activity after an acute and subchronic exposure to azinphos-methyl in the freshwater gastropod Biomphalaria straminea |
| title_short |
Resistance in cholinesterase activity after an acute and subchronic exposure to azinphos-methyl in the freshwater gastropod Biomphalaria straminea |
| title_full |
Resistance in cholinesterase activity after an acute and subchronic exposure to azinphos-methyl in the freshwater gastropod Biomphalaria straminea |
| title_fullStr |
Resistance in cholinesterase activity after an acute and subchronic exposure to azinphos-methyl in the freshwater gastropod Biomphalaria straminea |
| title_full_unstemmed |
Resistance in cholinesterase activity after an acute and subchronic exposure to azinphos-methyl in the freshwater gastropod Biomphalaria straminea |
| title_sort |
resistance in cholinesterase activity after an acute and subchronic exposure to azinphos-methyl in the freshwater gastropod biomphalaria straminea |
| publishDate |
2014 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01476513_v109_n_p85_Bianco http://hdl.handle.net/20.500.12110/paper_01476513_v109_n_p85_Bianco |
| work_keys_str_mv |
AT nahabediandanieleduardo resistanceincholinesteraseactivityafteranacuteandsubchronicexposuretoazinphosmethylinthefreshwatergastropodbiomphalariastraminea AT kristoffgisela resistanceincholinesteraseactivityafteranacuteandsubchronicexposuretoazinphosmethylinthefreshwatergastropodbiomphalariastraminea |
| _version_ |
1768545641482420224 |
| spelling |
paper:paper_01476513_v109_n_p85_Bianco2023-06-08T15:12:48Z Resistance in cholinesterase activity after an acute and subchronic exposure to azinphos-methyl in the freshwater gastropod Biomphalaria straminea Nahabedian, Daniel Eduardo Kristoff, Gisela Biomarkers Carboxylesterases Cholinesterases Organophosphorous insecticides Proteins 1,5 bis(4 allyldimethylammoniumphenyl)pentan 3 one dibromide acetylthiocholine azinphos methyl butyrylthiocholine carboxylesterase choline derivative cholinesterase cholinesterase inhibitor iso OMPA physostigmine propionylthiocholine unclassified drug azinphos methyl biological marker carboxylesterase cholinesterase insecticide water pollutant acetylthiocholine biological marker butyrylthiocholine choline derivative fresh water propionylthiocholine azinphos methyl insecticide azinphos methyl biomarker biomonitoring carbamate (ester) concentration (composition) enzyme activity gastropod insecticide protein toxicity nontarget organism pollution exposure animal tissue article bioassay Biomphalaria straminea concentration (parameters) controlled study enzyme activity enzyme inhibition enzyme kinetics exposure hydrolysis IC 50 in vitro study nonhuman protein content sensitivity analysis survival tissue homogenate animal Biomphalaria drug effects environmental exposure environmental monitoring enzymology metabolism toxicity water pollutant Article biological monitoring IC50 neurotoxicity protein analysis protein function protein hydrolysis protein metabolism Argentina Patagonia Animals Azinphosmethyl Biological Markers Biomphalaria Carboxylic Ester Hydrolases Cholinesterases Environmental Exposure Environmental Monitoring Insecticides Water Pollutants, Chemical Biomphalaria straminea Gastropoda Organophosphorous and carbamates insecticides are ones of the most popular classes of pesticides used in agriculture. Its success relies on their high acute toxicity and rapid environmental degradation. These insecticides inhibit cholinesterase and cause severe effects on aquatic non-target species, particularly in invertebrates. Since the properties of cholinesterases may differ between species, it is necessary to characterize them before their use as biomarkers. Also organophosphorous and carbamates inhibit carboxylesterases and the use of both enzymes for biomonitoring is suggested. Azinphos-methyl is an organophosphorous insecticide used in several parts of the word. In Argentina, it is the most applied insecticide in fruit production in the north Patagonian region. It was detected with the highest frequency in superficial and groundwater of the region. This work aims to evaluate the sensitivity of B. straminea cholinesterases and carboxylesterases to the OP azinphos-methyl including estimations of 48h NOEC and IC50 of the pesticide and subchronic effects at environmentally relevant concentrations. These will allow us to evaluate the possibility of using cholinesterase and carboxylesterase of B. straminea as sensitive biomarkers. Previously a partial characterization of these enzymes will be performed. As in most invertebrates, acetylthiocholine was the preferred hydrolyzed substrate of B. straminea ChE, followed by propionylthiocholine and being butyrylthiocholine hydrolysis very low. Cholinesterase activity of B. straminea was significantly inhibited by the selective cholinesterases inhibitor (eserine) and by the selective inhibitor of mammalian acethylcholinesterase (BW284c51). In contrast, iso-OMPA, a specific inhibitor of butyrylcholinesterase, did not inhibit cholinesterase activity. These results suggest that cholinesterase activity in total soft tissue of B. straminea corresponds to acethylcholinesterase. Carboxylesterases activity was one order of magnitude higher than cholinesterase. A greater efficiency (Vmax/Km) was obtained using acetylthiocholine and p-nitrophenyl butyrate. Acute exposure to azinphos-methyl did not cause inhibition of cholinesterase activity until 10mgL-1 used. Carboxylesterases towards p-nitrophenyl butyrate was inhibited by azinphos-methyl being the IC502.20±0.75mgL-1 of azinphos-methyl. Subchronic exposure to environmental concentrations of azinphos-methyl (0.02 and 0.2mgL-1) produced a decrease in survival, protein content and carboxylesterases activity despite no inhibition of cholinesterase activity was observed. B. straminea cholinesterase is not a sensible biomarker. On the contrary, carboxylesterases activity was inhibited by azinphos-methyl. Carboxylesterases could be protecting cholinesterase activity and therefore, protecting the organism from neurotoxicity. This work confirms the advantages of measuring cholinesterases and carboxylesterases jointly in aquatic biomonitoring of pesticide contamination. This becomes relevant in order to find more sensitive biomarkers and new strategies to protect non-target aquatic organisms from pesticide contamination. © 2014 Elsevier Inc. Fil:Nahabedian, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Kristoff, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2014 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01476513_v109_n_p85_Bianco http://hdl.handle.net/20.500.12110/paper_01476513_v109_n_p85_Bianco |