The polyhydroxyalkanoate genes of a stress resistant Antarctic Pseudomonas are situated within a genomic island
Pseudomonas sp. 14-3 is an Antarctic bacterium that shows high stress resistance in association with high polyhydroxybutyrate (PHB) production. In this paper genes involved in PHB biosynthesis (phaRBAC) were found within a genomic island named pha-GI. Numerous mobile elements or proteins associated...
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2007
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paper:paper_0147619X_v58_n3_p240_Ayub2023-06-08T15:12:47Z The polyhydroxyalkanoate genes of a stress resistant Antarctic Pseudomonas are situated within a genomic island Ayub, Nicolás Daniel Pettinari, María Julia Méndez, Beatriz Silvia Antarctica Genomic islands Polyhydroxyalkanoates Pseudomonas bacterial protein polyhydroxyalkanoic acid adaptation syndrome Antarctica article Azotobacter vinelandii bacterial gene bacterial genome bacterial strain Burkholderia codon usage gene insertion sequence gene sequence genetic variability horizontal gene transfer intron molecular phylogeny nucleotide sequence open reading frame plasmid protein secretion Proteobacteria sequence homology unindexed sequence Acyltransferases Antarctic Regions Bacterial Proteins Base Composition Base Sequence Cloning, Molecular DNA-Binding Proteins Genes, Bacterial Genomic Islands Hydroxybutyrates Molecular Sequence Data Open Reading Frames Phylogeny Polyhydroxyalkanoates Pseudomonas Repressor Proteins Sequence Alignment Azotobacter vinelandii Bacteria (microorganisms) Burkholderiales Proteobacteria Pseudomonas Pseudomonas sp. 14-3 Pseudomonas sp. 14-3 is an Antarctic bacterium that shows high stress resistance in association with high polyhydroxybutyrate (PHB) production. In this paper genes involved in PHB biosynthesis (phaRBAC) were found within a genomic island named pha-GI. Numerous mobile elements or proteins associated with them, such as an integrase, insertion sequences, a bacterial group II intron, a complete Type I protein secretion system and IncP plasmid-related proteins were detected among the 28 ORFs identified in this large genetic element (32.3 kb). The G + C distribution was not homogeneous, likely reflecting a mosaic structure that contains regions from diverse origins. pha-GI has strong similarities with genomic islands found in diverse Proteobacteria, including Burkholderiales species and Azotobacter vinelandii. The G + C content, phylogeny inference and codon usage analysis showed that the phaBAC cluster itself has a complex mosaic structure and indicated that the phaB and phaC genes were acquired by horizontal transfer, probably derived from Burkholderiales. These results describe for the first time a pha cluster located within a genomic island, and suggest that horizontal transfer of pha genes is a mechanism of adaptability to stress conditions such as those found in the extreme Antarctic environment. © 2007 Elsevier Inc. All rights reserved. Fil:Ayub, N.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pettinari, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Méndez, B.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2007 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0147619X_v58_n3_p240_Ayub http://hdl.handle.net/20.500.12110/paper_0147619X_v58_n3_p240_Ayub |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Antarctica Genomic islands Polyhydroxyalkanoates Pseudomonas bacterial protein polyhydroxyalkanoic acid adaptation syndrome Antarctica article Azotobacter vinelandii bacterial gene bacterial genome bacterial strain Burkholderia codon usage gene insertion sequence gene sequence genetic variability horizontal gene transfer intron molecular phylogeny nucleotide sequence open reading frame plasmid protein secretion Proteobacteria sequence homology unindexed sequence Acyltransferases Antarctic Regions Bacterial Proteins Base Composition Base Sequence Cloning, Molecular DNA-Binding Proteins Genes, Bacterial Genomic Islands Hydroxybutyrates Molecular Sequence Data Open Reading Frames Phylogeny Polyhydroxyalkanoates Pseudomonas Repressor Proteins Sequence Alignment Azotobacter vinelandii Bacteria (microorganisms) Burkholderiales Proteobacteria Pseudomonas Pseudomonas sp. 14-3 |
| spellingShingle |
Antarctica Genomic islands Polyhydroxyalkanoates Pseudomonas bacterial protein polyhydroxyalkanoic acid adaptation syndrome Antarctica article Azotobacter vinelandii bacterial gene bacterial genome bacterial strain Burkholderia codon usage gene insertion sequence gene sequence genetic variability horizontal gene transfer intron molecular phylogeny nucleotide sequence open reading frame plasmid protein secretion Proteobacteria sequence homology unindexed sequence Acyltransferases Antarctic Regions Bacterial Proteins Base Composition Base Sequence Cloning, Molecular DNA-Binding Proteins Genes, Bacterial Genomic Islands Hydroxybutyrates Molecular Sequence Data Open Reading Frames Phylogeny Polyhydroxyalkanoates Pseudomonas Repressor Proteins Sequence Alignment Azotobacter vinelandii Bacteria (microorganisms) Burkholderiales Proteobacteria Pseudomonas Pseudomonas sp. 14-3 Ayub, Nicolás Daniel Pettinari, María Julia Méndez, Beatriz Silvia The polyhydroxyalkanoate genes of a stress resistant Antarctic Pseudomonas are situated within a genomic island |
| topic_facet |
Antarctica Genomic islands Polyhydroxyalkanoates Pseudomonas bacterial protein polyhydroxyalkanoic acid adaptation syndrome Antarctica article Azotobacter vinelandii bacterial gene bacterial genome bacterial strain Burkholderia codon usage gene insertion sequence gene sequence genetic variability horizontal gene transfer intron molecular phylogeny nucleotide sequence open reading frame plasmid protein secretion Proteobacteria sequence homology unindexed sequence Acyltransferases Antarctic Regions Bacterial Proteins Base Composition Base Sequence Cloning, Molecular DNA-Binding Proteins Genes, Bacterial Genomic Islands Hydroxybutyrates Molecular Sequence Data Open Reading Frames Phylogeny Polyhydroxyalkanoates Pseudomonas Repressor Proteins Sequence Alignment Azotobacter vinelandii Bacteria (microorganisms) Burkholderiales Proteobacteria Pseudomonas Pseudomonas sp. 14-3 |
| description |
Pseudomonas sp. 14-3 is an Antarctic bacterium that shows high stress resistance in association with high polyhydroxybutyrate (PHB) production. In this paper genes involved in PHB biosynthesis (phaRBAC) were found within a genomic island named pha-GI. Numerous mobile elements or proteins associated with them, such as an integrase, insertion sequences, a bacterial group II intron, a complete Type I protein secretion system and IncP plasmid-related proteins were detected among the 28 ORFs identified in this large genetic element (32.3 kb). The G + C distribution was not homogeneous, likely reflecting a mosaic structure that contains regions from diverse origins. pha-GI has strong similarities with genomic islands found in diverse Proteobacteria, including Burkholderiales species and Azotobacter vinelandii. The G + C content, phylogeny inference and codon usage analysis showed that the phaBAC cluster itself has a complex mosaic structure and indicated that the phaB and phaC genes were acquired by horizontal transfer, probably derived from Burkholderiales. These results describe for the first time a pha cluster located within a genomic island, and suggest that horizontal transfer of pha genes is a mechanism of adaptability to stress conditions such as those found in the extreme Antarctic environment. © 2007 Elsevier Inc. All rights reserved. |
| author |
Ayub, Nicolás Daniel Pettinari, María Julia Méndez, Beatriz Silvia |
| author_facet |
Ayub, Nicolás Daniel Pettinari, María Julia Méndez, Beatriz Silvia |
| author_sort |
Ayub, Nicolás Daniel |
| title |
The polyhydroxyalkanoate genes of a stress resistant Antarctic Pseudomonas are situated within a genomic island |
| title_short |
The polyhydroxyalkanoate genes of a stress resistant Antarctic Pseudomonas are situated within a genomic island |
| title_full |
The polyhydroxyalkanoate genes of a stress resistant Antarctic Pseudomonas are situated within a genomic island |
| title_fullStr |
The polyhydroxyalkanoate genes of a stress resistant Antarctic Pseudomonas are situated within a genomic island |
| title_full_unstemmed |
The polyhydroxyalkanoate genes of a stress resistant Antarctic Pseudomonas are situated within a genomic island |
| title_sort |
polyhydroxyalkanoate genes of a stress resistant antarctic pseudomonas are situated within a genomic island |
| publishDate |
2007 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0147619X_v58_n3_p240_Ayub http://hdl.handle.net/20.500.12110/paper_0147619X_v58_n3_p240_Ayub |
| work_keys_str_mv |
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| _version_ |
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