A major transcript in the response of Neurospora crassa to protein synthesis inhibition by cycloheximide
In Neurospora crassa grown in minimal medium with 2 μM cycloheximide, the expression of several genes was shown to be turned on. We have isolated one of those genes coding for a major transcript in this response, which newly appears after addition of cycloheximide and remains present at a high level...
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1989
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Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01475975_v13_n2_p121_Aisemberg http://hdl.handle.net/20.500.12110/paper_01475975_v13_n2_p121_Aisemberg |
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paper:paper_01475975_v13_n2_p121_Aisemberg2023-06-08T15:12:44Z A major transcript in the response of Neurospora crassa to protein synthesis inhibition by cycloheximide Aisemberg, Gabriel Oscar Grotewold, Erich Taccioli, Guillermo Judewicz, Norberto D. cycloheximide gene expression Neurospora crassa stress proteins In Neurospora crassa grown in minimal medium with 2 μM cycloheximide, the expression of several genes was shown to be turned on. We have isolated one of those genes coding for a major transcript in this response, which newly appears after addition of cycloheximide and remains present at a high level for a long period. An open coding region for a 70-kDa protein was demonstrated by hybrid-selected translation, in agreement with the in vivo labeling of a protein with the same mobility in cycloheximide-induced cultures. Even 48 h after cycloheximide addition, CI-1-specific mRNA was bound to polysomal fractions. Our results also suggest that DNA sequences analogous to N. crassa CI-1 gene are present in other species. In N. crassa, CI-1 gene would not be induced by heat shock or starvation. © 1989. Fil:Aisemberg, G.O. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Grotewold, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Taccioli, G.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Judewicz, N.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1989 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01475975_v13_n2_p121_Aisemberg http://hdl.handle.net/20.500.12110/paper_01475975_v13_n2_p121_Aisemberg |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
cycloheximide gene expression Neurospora crassa stress proteins |
spellingShingle |
cycloheximide gene expression Neurospora crassa stress proteins Aisemberg, Gabriel Oscar Grotewold, Erich Taccioli, Guillermo Judewicz, Norberto D. A major transcript in the response of Neurospora crassa to protein synthesis inhibition by cycloheximide |
topic_facet |
cycloheximide gene expression Neurospora crassa stress proteins |
description |
In Neurospora crassa grown in minimal medium with 2 μM cycloheximide, the expression of several genes was shown to be turned on. We have isolated one of those genes coding for a major transcript in this response, which newly appears after addition of cycloheximide and remains present at a high level for a long period. An open coding region for a 70-kDa protein was demonstrated by hybrid-selected translation, in agreement with the in vivo labeling of a protein with the same mobility in cycloheximide-induced cultures. Even 48 h after cycloheximide addition, CI-1-specific mRNA was bound to polysomal fractions. Our results also suggest that DNA sequences analogous to N. crassa CI-1 gene are present in other species. In N. crassa, CI-1 gene would not be induced by heat shock or starvation. © 1989. |
author |
Aisemberg, Gabriel Oscar Grotewold, Erich Taccioli, Guillermo Judewicz, Norberto D. |
author_facet |
Aisemberg, Gabriel Oscar Grotewold, Erich Taccioli, Guillermo Judewicz, Norberto D. |
author_sort |
Aisemberg, Gabriel Oscar |
title |
A major transcript in the response of Neurospora crassa to protein synthesis inhibition by cycloheximide |
title_short |
A major transcript in the response of Neurospora crassa to protein synthesis inhibition by cycloheximide |
title_full |
A major transcript in the response of Neurospora crassa to protein synthesis inhibition by cycloheximide |
title_fullStr |
A major transcript in the response of Neurospora crassa to protein synthesis inhibition by cycloheximide |
title_full_unstemmed |
A major transcript in the response of Neurospora crassa to protein synthesis inhibition by cycloheximide |
title_sort |
major transcript in the response of neurospora crassa to protein synthesis inhibition by cycloheximide |
publishDate |
1989 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01475975_v13_n2_p121_Aisemberg http://hdl.handle.net/20.500.12110/paper_01475975_v13_n2_p121_Aisemberg |
work_keys_str_mv |
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