Endogenous and exogenous porphyrins as photosensitizers in the Hep-2 human carcinoma cell line

The photodynamic activity of three photosensitizers (PS): ALA-induced PPIX, the porphyrin derivative 5-(4-trimethylammoniumphenyl)-10, 5, 20-tris (2,4,6- trimethoxyphenyl) porphyrin (CP) and the molecular dyad porphyrin-C60 (P-C60), the last two incorporated into liposomal vesicles, was evaluated on...

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Autores principales:	Batlle, Alcira María del Carmen, Fukuda, Haydeé
Publicado:	2009
Materias:	5 aminolevulinic acid Apoptosis Cancer Necrosis Photodynamic therapy Photosensitization Phototherapeutic agents PPIX 5 (4 trimethylammoniumphenyl) 10,5,20 tris (2,4,6 trimethoxyphenyl) porphyrin antineoplastic agent unclassified drug apoptosis article cancer cell culture cell death cell structure cellular distribution controlled study cytoplasm HEp 2 cell human human cell photodynamic therapy photosensitization Aminolevulinic Acid Carcinoma Cell Line, Tumor Cell Survival Fullerenes Humans Laryngeal Neoplasms Light Liposomes Microscopy, Fluorescence Photochemotherapy Photosensitizing Agents Porphyrins Protoporphyrins
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01455680_v55_n2_p8_Alvarez http://hdl.handle.net/20.500.12110/paper_01455680_v55_n2_p8_Alvarez
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	paper:paper_01455680_v55_n2_p8_Alvarez2023-06-08T15:12:28Z Endogenous and exogenous porphyrins as photosensitizers in the Hep-2 human carcinoma cell line Batlle, Alcira María del Carmen Fukuda, Haydeé 5 aminolevulinic acid Apoptosis Cancer Necrosis Photodynamic therapy Photosensitization Phototherapeutic agents PPIX 5 (4 trimethylammoniumphenyl) 10,5,20 tris (2,4,6 trimethoxyphenyl) porphyrin antineoplastic agent unclassified drug apoptosis article cancer cell culture cell death cell structure cellular distribution controlled study cytoplasm HEp 2 cell human human cell photodynamic therapy photosensitization Aminolevulinic Acid Carcinoma Cell Line, Tumor Cell Survival Fullerenes Humans Laryngeal Neoplasms Light Liposomes Microscopy, Fluorescence Photochemotherapy Photosensitizing Agents Porphyrins Protoporphyrins The photodynamic activity of three photosensitizers (PS): ALA-induced PPIX, the porphyrin derivative 5-(4-trimethylammoniumphenyl)-10, 5, 20-tris (2,4,6- trimethoxyphenyl) porphyrin (CP) and the molecular dyad porphyrin-C60 (P-C60), the last two incorporated into liposomal vesicles, was evaluated on Hep-2 human larynx carcinoma cell line. ALAinduced accumulation of the endogenous PS PPIX, reached saturation values between 5 and 24 h incubation time; the maximal PPIX content was 5.7 nmol/106 cells. The same intracellular level was accumulated when the cationic porphyrin CP was used, while the amount of P-C60 attained was 1.5 nmol/106 cells. Under violet-blue exciting light, the fluorescence of PPIX and P-C60 was found in the cytoplasm showing a granular appearance indicating lysosomal localization. CP was mainly detected as a filamentous pattern characteristic of mitochondrial localization. No dark cytotoxicity was observed using 1mM ALA, 5 μM CP and 1 μM P-C60 after 24 h incubation. Cell morphology was analyzed using Hoechst-33258, toluidine blue staining, TUNEL assay and DNA fragmentation, 24 h after irradiation with 54 J/cm2. When photosensitized with ALA and P-C60, chromatine condensation characteristic of apoptotic cell death was found; instead, 58 % of necrotic cells were observed with CP. The results show that in the Hep-2 cells, of the three PS analyzed, the molecular dyad P-C60 was more efficient than CP and PPIX, and confirm that PDT can induce different mechanisms of cell death depending on the PS and the irradiation dose. Copyright © 2009 C.M.B. Edition. Fil:Batlle, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Fukuda, H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2009 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01455680_v55_n2_p8_Alvarez http://hdl.handle.net/20.500.12110/paper_01455680_v55_n2_p8_Alvarez
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	5 aminolevulinic acid Apoptosis Cancer Necrosis Photodynamic therapy Photosensitization Phototherapeutic agents PPIX 5 (4 trimethylammoniumphenyl) 10,5,20 tris (2,4,6 trimethoxyphenyl) porphyrin antineoplastic agent unclassified drug apoptosis article cancer cell culture cell death cell structure cellular distribution controlled study cytoplasm HEp 2 cell human human cell photodynamic therapy photosensitization Aminolevulinic Acid Carcinoma Cell Line, Tumor Cell Survival Fullerenes Humans Laryngeal Neoplasms Light Liposomes Microscopy, Fluorescence Photochemotherapy Photosensitizing Agents Porphyrins Protoporphyrins
spellingShingle	5 aminolevulinic acid Apoptosis Cancer Necrosis Photodynamic therapy Photosensitization Phototherapeutic agents PPIX 5 (4 trimethylammoniumphenyl) 10,5,20 tris (2,4,6 trimethoxyphenyl) porphyrin antineoplastic agent unclassified drug apoptosis article cancer cell culture cell death cell structure cellular distribution controlled study cytoplasm HEp 2 cell human human cell photodynamic therapy photosensitization Aminolevulinic Acid Carcinoma Cell Line, Tumor Cell Survival Fullerenes Humans Laryngeal Neoplasms Light Liposomes Microscopy, Fluorescence Photochemotherapy Photosensitizing Agents Porphyrins Protoporphyrins Batlle, Alcira María del Carmen Fukuda, Haydeé Endogenous and exogenous porphyrins as photosensitizers in the Hep-2 human carcinoma cell line
topic_facet	5 aminolevulinic acid Apoptosis Cancer Necrosis Photodynamic therapy Photosensitization Phototherapeutic agents PPIX 5 (4 trimethylammoniumphenyl) 10,5,20 tris (2,4,6 trimethoxyphenyl) porphyrin antineoplastic agent unclassified drug apoptosis article cancer cell culture cell death cell structure cellular distribution controlled study cytoplasm HEp 2 cell human human cell photodynamic therapy photosensitization Aminolevulinic Acid Carcinoma Cell Line, Tumor Cell Survival Fullerenes Humans Laryngeal Neoplasms Light Liposomes Microscopy, Fluorescence Photochemotherapy Photosensitizing Agents Porphyrins Protoporphyrins
description	The photodynamic activity of three photosensitizers (PS): ALA-induced PPIX, the porphyrin derivative 5-(4-trimethylammoniumphenyl)-10, 5, 20-tris (2,4,6- trimethoxyphenyl) porphyrin (CP) and the molecular dyad porphyrin-C60 (P-C60), the last two incorporated into liposomal vesicles, was evaluated on Hep-2 human larynx carcinoma cell line. ALAinduced accumulation of the endogenous PS PPIX, reached saturation values between 5 and 24 h incubation time; the maximal PPIX content was 5.7 nmol/106 cells. The same intracellular level was accumulated when the cationic porphyrin CP was used, while the amount of P-C60 attained was 1.5 nmol/106 cells. Under violet-blue exciting light, the fluorescence of PPIX and P-C60 was found in the cytoplasm showing a granular appearance indicating lysosomal localization. CP was mainly detected as a filamentous pattern characteristic of mitochondrial localization. No dark cytotoxicity was observed using 1mM ALA, 5 μM CP and 1 μM P-C60 after 24 h incubation. Cell morphology was analyzed using Hoechst-33258, toluidine blue staining, TUNEL assay and DNA fragmentation, 24 h after irradiation with 54 J/cm2. When photosensitized with ALA and P-C60, chromatine condensation characteristic of apoptotic cell death was found; instead, 58 % of necrotic cells were observed with CP. The results show that in the Hep-2 cells, of the three PS analyzed, the molecular dyad P-C60 was more efficient than CP and PPIX, and confirm that PDT can induce different mechanisms of cell death depending on the PS and the irradiation dose. Copyright © 2009 C.M.B. Edition.
author	Batlle, Alcira María del Carmen Fukuda, Haydeé
author_facet	Batlle, Alcira María del Carmen Fukuda, Haydeé
author_sort	Batlle, Alcira María del Carmen
title	Endogenous and exogenous porphyrins as photosensitizers in the Hep-2 human carcinoma cell line
title_short	Endogenous and exogenous porphyrins as photosensitizers in the Hep-2 human carcinoma cell line
title_full	Endogenous and exogenous porphyrins as photosensitizers in the Hep-2 human carcinoma cell line
title_fullStr	Endogenous and exogenous porphyrins as photosensitizers in the Hep-2 human carcinoma cell line
title_full_unstemmed	Endogenous and exogenous porphyrins as photosensitizers in the Hep-2 human carcinoma cell line
title_sort	endogenous and exogenous porphyrins as photosensitizers in the hep-2 human carcinoma cell line
publishDate	2009
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01455680_v55_n2_p8_Alvarez http://hdl.handle.net/20.500.12110/paper_01455680_v55_n2_p8_Alvarez
work_keys_str_mv	AT batllealciramariadelcarmen endogenousandexogenousporphyrinsasphotosensitizersinthehep2humancarcinomacellline AT fukudahaydee endogenousandexogenousporphyrinsasphotosensitizersinthehep2humancarcinomacellline
_version_	1768546203245477888

Endogenous and exogenous porphyrins as photosensitizers in the Hep-2 human carcinoma cell line

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