ROS production by endogenously generated protoporphyrin IX in murine leukemia cells
Endogenous production of Protoporphyrin IX (PpIX) is successfully exploited for photodynamic therapy (PDT) on malignant cells, following 5-aminolevulinic acid (ALA) administration and light irradiation. This treatment kills cancer cells by damaging organelles and impairing metabolic pathways via cel...
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Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01455680_v55_n2_p15_Diez http://hdl.handle.net/20.500.12110/paper_01455680_v55_n2_p15_Diez |
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paper:paper_01455680_v55_n2_p15_Diez2023-06-08T15:12:28Z ROS production by endogenously generated protoporphyrin IX in murine leukemia cells Diez, Berenice Andrea Teijo, María Julieta Batlle, Alcira María del Carmen Fukuda, Haydeé 5-aminolevulinic acid Photodynamic therapy Porphyrins aminolevulinic acid dichlorodihydrofluorescein diacetate doxorubicin hydroethidine protoporphyrin reactive oxygen metabolite superoxide vincristine apoptosis article cancer cell culture cell damage controlled study flow cytometry human human cell leukemia cell mitochondrial membrane potential mitochondrion photodynamic therapy Aminolevulinic Acid Animals Apoptosis Cell Line, Tumor Drug Resistance, Neoplasm Leukemia Membrane Potential, Mitochondrial Mice Mitochondria Photochemotherapy Protoporphyrins Reactive Oxygen Species Superoxides Ultraviolet Rays Murinae Endogenous production of Protoporphyrin IX (PpIX) is successfully exploited for photodynamic therapy (PDT) on malignant cells, following 5-aminolevulinic acid (ALA) administration and light irradiation. This treatment kills cancer cells by damaging organelles and impairing metabolic pathways via cellular reactive oxygen species (ROS) generation. We studied the efficiency of PpIX synthetized from ALA on ROS generation, in the Vincristine resistant (LBR-V160), Doxorubicin resistant (LBR-D160) and sensitive (LBR-) murine leukemia cell lines. Cells were incubated 4 hr with 1 mM ALA and then irradiated during different times with fluorescent light. One hour later, production of ROS was analyzed by flow cytometry using different fluorescent probes: Hydroethidine (HE) for superoxide anion, 2',7' Dichlorodihydrofluorescein diacetate (DCFH-DA) for hydrogen peroxide; mitochondrial damage was examined with 3,3' Dihexyloxacarbocyanine iodide (DiOC6). We found that superoxide anion production in the three cell lines increased with irradiation time whereas no peroxide hydrogen was detected. Mitochondrial damage also increased in an irradiation time dependent manner, being higher in the Vincristine resistant line. Previous studies have demonstrated that apoptotic cell death increased with irradiation time, which is consistent with these results, indicating that ROS are critical in ALA-PDT efficiency to kill malignant cells. Copyright © 2009 C.M.B. Edition. Fil:Diez, B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Teijo, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Batlle, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Fukuda, H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2009 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01455680_v55_n2_p15_Diez http://hdl.handle.net/20.500.12110/paper_01455680_v55_n2_p15_Diez |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
5-aminolevulinic acid Photodynamic therapy Porphyrins aminolevulinic acid dichlorodihydrofluorescein diacetate doxorubicin hydroethidine protoporphyrin reactive oxygen metabolite superoxide vincristine apoptosis article cancer cell culture cell damage controlled study flow cytometry human human cell leukemia cell mitochondrial membrane potential mitochondrion photodynamic therapy Aminolevulinic Acid Animals Apoptosis Cell Line, Tumor Drug Resistance, Neoplasm Leukemia Membrane Potential, Mitochondrial Mice Mitochondria Photochemotherapy Protoporphyrins Reactive Oxygen Species Superoxides Ultraviolet Rays Murinae |
spellingShingle |
5-aminolevulinic acid Photodynamic therapy Porphyrins aminolevulinic acid dichlorodihydrofluorescein diacetate doxorubicin hydroethidine protoporphyrin reactive oxygen metabolite superoxide vincristine apoptosis article cancer cell culture cell damage controlled study flow cytometry human human cell leukemia cell mitochondrial membrane potential mitochondrion photodynamic therapy Aminolevulinic Acid Animals Apoptosis Cell Line, Tumor Drug Resistance, Neoplasm Leukemia Membrane Potential, Mitochondrial Mice Mitochondria Photochemotherapy Protoporphyrins Reactive Oxygen Species Superoxides Ultraviolet Rays Murinae Diez, Berenice Andrea Teijo, María Julieta Batlle, Alcira María del Carmen Fukuda, Haydeé ROS production by endogenously generated protoporphyrin IX in murine leukemia cells |
topic_facet |
5-aminolevulinic acid Photodynamic therapy Porphyrins aminolevulinic acid dichlorodihydrofluorescein diacetate doxorubicin hydroethidine protoporphyrin reactive oxygen metabolite superoxide vincristine apoptosis article cancer cell culture cell damage controlled study flow cytometry human human cell leukemia cell mitochondrial membrane potential mitochondrion photodynamic therapy Aminolevulinic Acid Animals Apoptosis Cell Line, Tumor Drug Resistance, Neoplasm Leukemia Membrane Potential, Mitochondrial Mice Mitochondria Photochemotherapy Protoporphyrins Reactive Oxygen Species Superoxides Ultraviolet Rays Murinae |
description |
Endogenous production of Protoporphyrin IX (PpIX) is successfully exploited for photodynamic therapy (PDT) on malignant cells, following 5-aminolevulinic acid (ALA) administration and light irradiation. This treatment kills cancer cells by damaging organelles and impairing metabolic pathways via cellular reactive oxygen species (ROS) generation. We studied the efficiency of PpIX synthetized from ALA on ROS generation, in the Vincristine resistant (LBR-V160), Doxorubicin resistant (LBR-D160) and sensitive (LBR-) murine leukemia cell lines. Cells were incubated 4 hr with 1 mM ALA and then irradiated during different times with fluorescent light. One hour later, production of ROS was analyzed by flow cytometry using different fluorescent probes: Hydroethidine (HE) for superoxide anion, 2',7' Dichlorodihydrofluorescein diacetate (DCFH-DA) for hydrogen peroxide; mitochondrial damage was examined with 3,3' Dihexyloxacarbocyanine iodide (DiOC6). We found that superoxide anion production in the three cell lines increased with irradiation time whereas no peroxide hydrogen was detected. Mitochondrial damage also increased in an irradiation time dependent manner, being higher in the Vincristine resistant line. Previous studies have demonstrated that apoptotic cell death increased with irradiation time, which is consistent with these results, indicating that ROS are critical in ALA-PDT efficiency to kill malignant cells. Copyright © 2009 C.M.B. Edition. |
author |
Diez, Berenice Andrea Teijo, María Julieta Batlle, Alcira María del Carmen Fukuda, Haydeé |
author_facet |
Diez, Berenice Andrea Teijo, María Julieta Batlle, Alcira María del Carmen Fukuda, Haydeé |
author_sort |
Diez, Berenice Andrea |
title |
ROS production by endogenously generated protoporphyrin IX in murine leukemia cells |
title_short |
ROS production by endogenously generated protoporphyrin IX in murine leukemia cells |
title_full |
ROS production by endogenously generated protoporphyrin IX in murine leukemia cells |
title_fullStr |
ROS production by endogenously generated protoporphyrin IX in murine leukemia cells |
title_full_unstemmed |
ROS production by endogenously generated protoporphyrin IX in murine leukemia cells |
title_sort |
ros production by endogenously generated protoporphyrin ix in murine leukemia cells |
publishDate |
2009 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01455680_v55_n2_p15_Diez http://hdl.handle.net/20.500.12110/paper_01455680_v55_n2_p15_Diez |
work_keys_str_mv |
AT diezbereniceandrea rosproductionbyendogenouslygeneratedprotoporphyrinixinmurineleukemiacells AT teijomariajulieta rosproductionbyendogenouslygeneratedprotoporphyrinixinmurineleukemiacells AT batllealciramariadelcarmen rosproductionbyendogenouslygeneratedprotoporphyrinixinmurineleukemiacells AT fukudahaydee rosproductionbyendogenouslygeneratedprotoporphyrinixinmurineleukemiacells |
_version_ |
1768546203056734208 |