Hepatic damage and oxidative stress induced by griseofulvin in mice

Erythropoietic Protoporphyria (EPP) is a disease associated with ferrochelatase deficiency, which produces accumulation of protoporphyrin IX (PROTO IX) in erythrocytes, liver and skin. In some cases, a severe hepatic failure and cholestasis was observed. Griseofulvin (Gris) develops an experimental...

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Autores principales:	Martínez, María del Carmen, Afonso, Susana Graciela, Buzaleh, Ana María, Batlle, Alcira María del Carmen
Publicado:	2009
Materias:	Erythropoietic Protoporphyria Griseofulvin Heme metabolism Liver damage Oxidative stress 5 aminolevulinate synthase alanine aminotransferase alkaline phosphatase aspartate aminotransferase catalase cytochrome P450 1A2 cytochrome P450 2A6 cytochrome P450 2C9 cytochrome P450 2E1 cytochrome P450 3A4 glutathione glutathione peroxidase glutathione reductase glutathione transferase griseofulvin heme heme oxygenase malonaldehyde protoporphyrin reactive oxygen metabolite superoxide dismutase tryptophan 2,3 dioxygenase alanine aminotransferase blood level alkaline phosphatase blood level animal experiment animal model animal tissue antioxidant activity article aspartate aminotransferase blood level blood level body weight controlled study enzyme activity enzyme induction erythropoietic protoporphyria experimental mouse feces level histopathology liver injury liver level liver weight male mouse nonhuman oxidation reduction reaction oxidative stress Animals Antifungal Agents Aryl Hydrocarbon Hydroxylases Cytochrome P-450 CYP3A Disease Models, Animal Heme Heme Oxygenase (Decyclizing) Immunohistochemistry Liver Male Mice Oxidative Stress Protoporphyria, Erythropoietic Protoporphyrins Tryptophan Oxygenase Animalia Mus
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01455680_v55_n2_p127_Martinez http://hdl.handle.net/20.500.12110/paper_01455680_v55_n2_p127_Martinez
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	paper:paper_01455680_v55_n2_p127_Martinez2023-06-08T15:12:27Z Hepatic damage and oxidative stress induced by griseofulvin in mice Martínez, María del Carmen Afonso, Susana Graciela Buzaleh, Ana María Batlle, Alcira María del Carmen Erythropoietic Protoporphyria Griseofulvin Heme metabolism Liver damage Oxidative stress 5 aminolevulinate synthase alanine aminotransferase alkaline phosphatase aspartate aminotransferase catalase cytochrome P450 1A2 cytochrome P450 2A6 cytochrome P450 2C9 cytochrome P450 2E1 cytochrome P450 3A4 glutathione glutathione peroxidase glutathione reductase glutathione transferase griseofulvin heme heme oxygenase malonaldehyde protoporphyrin reactive oxygen metabolite superoxide dismutase tryptophan 2,3 dioxygenase alanine aminotransferase blood level alkaline phosphatase blood level animal experiment animal model animal tissue antioxidant activity article aspartate aminotransferase blood level blood level body weight controlled study enzyme activity enzyme induction erythropoietic protoporphyria experimental mouse feces level histopathology liver injury liver level liver weight male mouse nonhuman oxidation reduction reaction oxidative stress Animals Antifungal Agents Aryl Hydrocarbon Hydroxylases Cytochrome P-450 CYP3A Disease Models, Animal Griseofulvin Heme Heme Oxygenase (Decyclizing) Immunohistochemistry Liver Male Mice Oxidative Stress Protoporphyria, Erythropoietic Protoporphyrins Tryptophan Oxygenase Animalia Mus Erythropoietic Protoporphyria (EPP) is a disease associated with ferrochelatase deficiency, which produces accumulation of protoporphyrin IX (PROTO IX) in erythrocytes, liver and skin. In some cases, a severe hepatic failure and cholestasis was observed. Griseofulvin (Gris) develops an experimental EPP with hepatic manifestations in animals. The aim of this work was to further characterize this model studying its effect on different metabolisms in mice Gris feeding (0-2.5%, 7 and 14 days). PROTO IX accumulation in liver, blood and feces, induction of ALA-S activity, and a low rate of Holo/Apo tryptophan pyrrolase activity was produced, indicating a reduction of free heme pool. The progressive liver injury was reflected by the aspect and the enlargement of liver and the induction of hepatic damage. Liver redox balance was altered due to porphyrin high concentrations; as a consequence, the antioxidant defense system was disrupted. Heme oxygenase was also induced, however, at higher concentrations of antifungal, the free heme pool would be so depleted that this enzyme would not be necessary. In conclusion, our model of Protoporphyria produced liver alterations similar to those found in EPP patients. Copyright © 2009 C.M.B. Edition. Fil:Martinez, M.D.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Afonso, S.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Buzaleh, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Batlle, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2009 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01455680_v55_n2_p127_Martinez http://hdl.handle.net/20.500.12110/paper_01455680_v55_n2_p127_Martinez
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Erythropoietic Protoporphyria Griseofulvin Heme metabolism Liver damage Oxidative stress 5 aminolevulinate synthase alanine aminotransferase alkaline phosphatase aspartate aminotransferase catalase cytochrome P450 1A2 cytochrome P450 2A6 cytochrome P450 2C9 cytochrome P450 2E1 cytochrome P450 3A4 glutathione glutathione peroxidase glutathione reductase glutathione transferase griseofulvin heme heme oxygenase malonaldehyde protoporphyrin reactive oxygen metabolite superoxide dismutase tryptophan 2,3 dioxygenase alanine aminotransferase blood level alkaline phosphatase blood level animal experiment animal model animal tissue antioxidant activity article aspartate aminotransferase blood level blood level body weight controlled study enzyme activity enzyme induction erythropoietic protoporphyria experimental mouse feces level histopathology liver injury liver level liver weight male mouse nonhuman oxidation reduction reaction oxidative stress Animals Antifungal Agents Aryl Hydrocarbon Hydroxylases Cytochrome P-450 CYP3A Disease Models, Animal Griseofulvin Heme Heme Oxygenase (Decyclizing) Immunohistochemistry Liver Male Mice Oxidative Stress Protoporphyria, Erythropoietic Protoporphyrins Tryptophan Oxygenase Animalia Mus
spellingShingle	Erythropoietic Protoporphyria Griseofulvin Heme metabolism Liver damage Oxidative stress 5 aminolevulinate synthase alanine aminotransferase alkaline phosphatase aspartate aminotransferase catalase cytochrome P450 1A2 cytochrome P450 2A6 cytochrome P450 2C9 cytochrome P450 2E1 cytochrome P450 3A4 glutathione glutathione peroxidase glutathione reductase glutathione transferase griseofulvin heme heme oxygenase malonaldehyde protoporphyrin reactive oxygen metabolite superoxide dismutase tryptophan 2,3 dioxygenase alanine aminotransferase blood level alkaline phosphatase blood level animal experiment animal model animal tissue antioxidant activity article aspartate aminotransferase blood level blood level body weight controlled study enzyme activity enzyme induction erythropoietic protoporphyria experimental mouse feces level histopathology liver injury liver level liver weight male mouse nonhuman oxidation reduction reaction oxidative stress Animals Antifungal Agents Aryl Hydrocarbon Hydroxylases Cytochrome P-450 CYP3A Disease Models, Animal Griseofulvin Heme Heme Oxygenase (Decyclizing) Immunohistochemistry Liver Male Mice Oxidative Stress Protoporphyria, Erythropoietic Protoporphyrins Tryptophan Oxygenase Animalia Mus Martínez, María del Carmen Afonso, Susana Graciela Buzaleh, Ana María Batlle, Alcira María del Carmen Hepatic damage and oxidative stress induced by griseofulvin in mice
topic_facet	Erythropoietic Protoporphyria Griseofulvin Heme metabolism Liver damage Oxidative stress 5 aminolevulinate synthase alanine aminotransferase alkaline phosphatase aspartate aminotransferase catalase cytochrome P450 1A2 cytochrome P450 2A6 cytochrome P450 2C9 cytochrome P450 2E1 cytochrome P450 3A4 glutathione glutathione peroxidase glutathione reductase glutathione transferase griseofulvin heme heme oxygenase malonaldehyde protoporphyrin reactive oxygen metabolite superoxide dismutase tryptophan 2,3 dioxygenase alanine aminotransferase blood level alkaline phosphatase blood level animal experiment animal model animal tissue antioxidant activity article aspartate aminotransferase blood level blood level body weight controlled study enzyme activity enzyme induction erythropoietic protoporphyria experimental mouse feces level histopathology liver injury liver level liver weight male mouse nonhuman oxidation reduction reaction oxidative stress Animals Antifungal Agents Aryl Hydrocarbon Hydroxylases Cytochrome P-450 CYP3A Disease Models, Animal Griseofulvin Heme Heme Oxygenase (Decyclizing) Immunohistochemistry Liver Male Mice Oxidative Stress Protoporphyria, Erythropoietic Protoporphyrins Tryptophan Oxygenase Animalia Mus
description	Erythropoietic Protoporphyria (EPP) is a disease associated with ferrochelatase deficiency, which produces accumulation of protoporphyrin IX (PROTO IX) in erythrocytes, liver and skin. In some cases, a severe hepatic failure and cholestasis was observed. Griseofulvin (Gris) develops an experimental EPP with hepatic manifestations in animals. The aim of this work was to further characterize this model studying its effect on different metabolisms in mice Gris feeding (0-2.5%, 7 and 14 days). PROTO IX accumulation in liver, blood and feces, induction of ALA-S activity, and a low rate of Holo/Apo tryptophan pyrrolase activity was produced, indicating a reduction of free heme pool. The progressive liver injury was reflected by the aspect and the enlargement of liver and the induction of hepatic damage. Liver redox balance was altered due to porphyrin high concentrations; as a consequence, the antioxidant defense system was disrupted. Heme oxygenase was also induced, however, at higher concentrations of antifungal, the free heme pool would be so depleted that this enzyme would not be necessary. In conclusion, our model of Protoporphyria produced liver alterations similar to those found in EPP patients. Copyright © 2009 C.M.B. Edition.
author	Martínez, María del Carmen Afonso, Susana Graciela Buzaleh, Ana María Batlle, Alcira María del Carmen
author_facet	Martínez, María del Carmen Afonso, Susana Graciela Buzaleh, Ana María Batlle, Alcira María del Carmen
author_sort	Martínez, María del Carmen
title	Hepatic damage and oxidative stress induced by griseofulvin in mice
title_short	Hepatic damage and oxidative stress induced by griseofulvin in mice
title_full	Hepatic damage and oxidative stress induced by griseofulvin in mice
title_fullStr	Hepatic damage and oxidative stress induced by griseofulvin in mice
title_full_unstemmed	Hepatic damage and oxidative stress induced by griseofulvin in mice
title_sort	hepatic damage and oxidative stress induced by griseofulvin in mice
publishDate	2009
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01455680_v55_n2_p127_Martinez http://hdl.handle.net/20.500.12110/paper_01455680_v55_n2_p127_Martinez
work_keys_str_mv	AT martinezmariadelcarmen hepaticdamageandoxidativestressinducedbygriseofulvininmice AT afonsosusanagraciela hepaticdamageandoxidativestressinducedbygriseofulvininmice AT buzalehanamaria hepaticdamageandoxidativestressinducedbygriseofulvininmice AT batllealciramariadelcarmen hepaticdamageandoxidativestressinducedbygriseofulvininmice
_version_	1768544034312159232

Hepatic damage and oxidative stress induced by griseofulvin in mice

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