In vitro photosensitisation of a murine mammary adenocarcinoma cell line with Verteporfin.
Benzoporphyrin derivative monoacid ring A (BPD-MA) is a second generation hydrophobic photosensitiser for PDT that has been approved for ocular disease treatment. In the present paper we report the results of in vitro studies on the photosensitising activity of Verteporfin (liposomally formulated BP...
Guardado en:
| Autores principales: | , , , |
|---|---|
| Publicado: |
2002
|
| Materias: | |
| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01455680_v48_n8_p931_Casas http://hdl.handle.net/20.500.12110/paper_01455680_v48_n8_p931_Casas |
| Aporte de: |
| id |
paper:paper_01455680_v48_n8_p931_Casas |
|---|---|
| record_format |
dspace |
| spelling |
paper:paper_01455680_v48_n8_p931_Casas2023-06-08T15:12:25Z In vitro photosensitisation of a murine mammary adenocarcinoma cell line with Verteporfin. Casas, Adriana Gabriela Di Venosa, Gabriela Mariana Batlle, Alcira María del Carmen Fukuda, Haydeé acridine orange benzoporphyrin derivative coloring agent fluorescent dye oxygen photosensitizing agent porphyrin reactive oxygen metabolite tetrazolium thiazole derivative thiazolyl blue tryptophan adenocarcinoma animal apoptosis article cell culture dose response experimental neoplasm fluorescence microscopy light metabolism mouse radiation response time Acridine Orange Adenocarcinoma Animals Apoptosis Coloring Agents Dose-Response Relationship, Drug Dose-Response Relationship, Radiation Fluorescent Dyes Light Mammary Neoplasms, Animal Mice Microscopy, Fluorescence Oxygen Photosensitizing Agents Porphyrins Reactive Oxygen Species Tetrazolium Salts Thiazoles Time Factors Tryptophan Tumor Cells, Cultured Benzoporphyrin derivative monoacid ring A (BPD-MA) is a second generation hydrophobic photosensitiser for PDT that has been approved for ocular disease treatment. In the present paper we report the results of in vitro studies on the photosensitising activity of Verteporfin (liposomally formulated BPD-MA) using an adenocarcinoma derived cell line. Our findings show a quick and efficient uptake of Verteporfin by LM3 cells, reaching maxima concentrations after 5 hr exposure to 18 microg Verteporfin/ml. Independently on the concentration, plateau levels are attained 5 hr after exposure to Verteporfin. Exposure of the cells to the photosensitiser appears to be safe in the darkness within a broad range of concentrations. The hydroxyl radical scavenger mannitol afforded the highest protection against PDT, while L-tryptophan, a well known and efficient singlet oxygen quencher was not an effective protector at all, showing scavenging activity only when it was supplemented at concentration as high as 10 mM and when 50% of the cells were affected, showing that in addition to singlet oxygen, which is considered the primary cytotoxic agent in PDT, other interconvertible reactive oxygen specie (ROS), in particular HO are also generated. Verteporfin-PDT also induced morphological features typical of apoptotic cells. Results of the present work show that the LM3 adenocarcinoma cell can be effectively sensitised with Verteporfin-PDT. Fil:Casas, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Di Venosa, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Batlle, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Fukuda, H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2002 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01455680_v48_n8_p931_Casas http://hdl.handle.net/20.500.12110/paper_01455680_v48_n8_p931_Casas |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
acridine orange benzoporphyrin derivative coloring agent fluorescent dye oxygen photosensitizing agent porphyrin reactive oxygen metabolite tetrazolium thiazole derivative thiazolyl blue tryptophan adenocarcinoma animal apoptosis article cell culture dose response experimental neoplasm fluorescence microscopy light metabolism mouse radiation response time Acridine Orange Adenocarcinoma Animals Apoptosis Coloring Agents Dose-Response Relationship, Drug Dose-Response Relationship, Radiation Fluorescent Dyes Light Mammary Neoplasms, Animal Mice Microscopy, Fluorescence Oxygen Photosensitizing Agents Porphyrins Reactive Oxygen Species Tetrazolium Salts Thiazoles Time Factors Tryptophan Tumor Cells, Cultured |
| spellingShingle |
acridine orange benzoporphyrin derivative coloring agent fluorescent dye oxygen photosensitizing agent porphyrin reactive oxygen metabolite tetrazolium thiazole derivative thiazolyl blue tryptophan adenocarcinoma animal apoptosis article cell culture dose response experimental neoplasm fluorescence microscopy light metabolism mouse radiation response time Acridine Orange Adenocarcinoma Animals Apoptosis Coloring Agents Dose-Response Relationship, Drug Dose-Response Relationship, Radiation Fluorescent Dyes Light Mammary Neoplasms, Animal Mice Microscopy, Fluorescence Oxygen Photosensitizing Agents Porphyrins Reactive Oxygen Species Tetrazolium Salts Thiazoles Time Factors Tryptophan Tumor Cells, Cultured Casas, Adriana Gabriela Di Venosa, Gabriela Mariana Batlle, Alcira María del Carmen Fukuda, Haydeé In vitro photosensitisation of a murine mammary adenocarcinoma cell line with Verteporfin. |
| topic_facet |
acridine orange benzoporphyrin derivative coloring agent fluorescent dye oxygen photosensitizing agent porphyrin reactive oxygen metabolite tetrazolium thiazole derivative thiazolyl blue tryptophan adenocarcinoma animal apoptosis article cell culture dose response experimental neoplasm fluorescence microscopy light metabolism mouse radiation response time Acridine Orange Adenocarcinoma Animals Apoptosis Coloring Agents Dose-Response Relationship, Drug Dose-Response Relationship, Radiation Fluorescent Dyes Light Mammary Neoplasms, Animal Mice Microscopy, Fluorescence Oxygen Photosensitizing Agents Porphyrins Reactive Oxygen Species Tetrazolium Salts Thiazoles Time Factors Tryptophan Tumor Cells, Cultured |
| description |
Benzoporphyrin derivative monoacid ring A (BPD-MA) is a second generation hydrophobic photosensitiser for PDT that has been approved for ocular disease treatment. In the present paper we report the results of in vitro studies on the photosensitising activity of Verteporfin (liposomally formulated BPD-MA) using an adenocarcinoma derived cell line. Our findings show a quick and efficient uptake of Verteporfin by LM3 cells, reaching maxima concentrations after 5 hr exposure to 18 microg Verteporfin/ml. Independently on the concentration, plateau levels are attained 5 hr after exposure to Verteporfin. Exposure of the cells to the photosensitiser appears to be safe in the darkness within a broad range of concentrations. The hydroxyl radical scavenger mannitol afforded the highest protection against PDT, while L-tryptophan, a well known and efficient singlet oxygen quencher was not an effective protector at all, showing scavenging activity only when it was supplemented at concentration as high as 10 mM and when 50% of the cells were affected, showing that in addition to singlet oxygen, which is considered the primary cytotoxic agent in PDT, other interconvertible reactive oxygen specie (ROS), in particular HO are also generated. Verteporfin-PDT also induced morphological features typical of apoptotic cells. Results of the present work show that the LM3 adenocarcinoma cell can be effectively sensitised with Verteporfin-PDT. |
| author |
Casas, Adriana Gabriela Di Venosa, Gabriela Mariana Batlle, Alcira María del Carmen Fukuda, Haydeé |
| author_facet |
Casas, Adriana Gabriela Di Venosa, Gabriela Mariana Batlle, Alcira María del Carmen Fukuda, Haydeé |
| author_sort |
Casas, Adriana Gabriela |
| title |
In vitro photosensitisation of a murine mammary adenocarcinoma cell line with Verteporfin. |
| title_short |
In vitro photosensitisation of a murine mammary adenocarcinoma cell line with Verteporfin. |
| title_full |
In vitro photosensitisation of a murine mammary adenocarcinoma cell line with Verteporfin. |
| title_fullStr |
In vitro photosensitisation of a murine mammary adenocarcinoma cell line with Verteporfin. |
| title_full_unstemmed |
In vitro photosensitisation of a murine mammary adenocarcinoma cell line with Verteporfin. |
| title_sort |
in vitro photosensitisation of a murine mammary adenocarcinoma cell line with verteporfin. |
| publishDate |
2002 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01455680_v48_n8_p931_Casas http://hdl.handle.net/20.500.12110/paper_01455680_v48_n8_p931_Casas |
| work_keys_str_mv |
AT casasadrianagabriela invitrophotosensitisationofamurinemammaryadenocarcinomacelllinewithverteporfin AT divenosagabrielamariana invitrophotosensitisationofamurinemammaryadenocarcinomacelllinewithverteporfin AT batllealciramariadelcarmen invitrophotosensitisationofamurinemammaryadenocarcinomacelllinewithverteporfin AT fukudahaydee invitrophotosensitisationofamurinemammaryadenocarcinomacelllinewithverteporfin |
| _version_ |
1768545368368218112 |