Effect of polyamines on plasmid-mediated kanamycin resistance and kanamycin phosphotransferase gene expression in Escherichia coli.

The emergence of kanamycin resistance in a polyamine-deficient mutant of E. coli transformed with a plasmid encoding the kanamycin phosphotransferase gene has been studied. The initial inhibition of growth and protein synthesis caused by the addition of the antibiotic could be reversed earlier in po...

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Detalles Bibliográficos
Autores principales: Nastri, Horacio Gabriel, Algranati, Israel David
Publicado: 1996
Materias:
bacterial protein
bacterial RNA
kanamycin kinase
messenger RNA
phosphotransferase
polyamine
putrescine
antibiotic resistance
article
bacterial gene
biosynthesis
drug effect
enzymology
Escherichia coli
gene expression
genetic transformation
genetics
kinetics
metabolism
plasmid
Bacterial Proteins
Gene Expression
Genes, Bacterial
Kanamycin Kinase
Kanamycin Resistance
Kinetics
Phosphotransferases (Alcohol Group Acceptor)
Plasmids
Polyamines
Putrescine
RNA, Bacterial
RNA, Messenger
Transformation, Genetic
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01455680_v42_n5_p711_Nastri
http://hdl.handle.net/20.500.12110/paper_01455680_v42_n5_p711_Nastri
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_01455680_v42_n5_p711_Nastri
record_format dspace
spelling paper:paper_01455680_v42_n5_p711_Nastri2023-06-08T15:12:21Z Effect of polyamines on plasmid-mediated kanamycin resistance and kanamycin phosphotransferase gene expression in Escherichia coli. Nastri, Horacio Gabriel Algranati, Israel David bacterial protein bacterial RNA kanamycin kinase messenger RNA phosphotransferase polyamine putrescine antibiotic resistance article bacterial gene biosynthesis drug effect enzymology Escherichia coli gene expression genetic transformation genetics kinetics metabolism plasmid Bacterial Proteins Escherichia coli Gene Expression Genes, Bacterial Kanamycin Kinase Kanamycin Resistance Kinetics Phosphotransferases (Alcohol Group Acceptor) Plasmids Polyamines Putrescine RNA, Bacterial RNA, Messenger Transformation, Genetic The emergence of kanamycin resistance in a polyamine-deficient mutant of E. coli transformed with a plasmid encoding the kanamycin phosphotransferase gene has been studied. The initial inhibition of growth and protein synthesis caused by the addition of the antibiotic could be reversed earlier in polyamine-supplemented bacteria than in those depleted of the organic bases. Concomitantly, we have observed that the increase of kanamycin phosphotransferase activity evoking the antibiotic resistance was higher in bacteria cultivated in the presence of putrescine. This result seems to depend exclusively on the enhanced capacity of the translation process in bacteria grown with polyamines since the transcription of phosphotransferase gene was higher in cells subjected to polyamine starvation. Fil:Nastri, H.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Algranati, I.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1996 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01455680_v42_n5_p711_Nastri http://hdl.handle.net/20.500.12110/paper_01455680_v42_n5_p711_Nastri
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic bacterial protein
bacterial RNA
kanamycin kinase
messenger RNA
phosphotransferase
polyamine
putrescine
antibiotic resistance
article
bacterial gene
biosynthesis
drug effect
enzymology
Escherichia coli
gene expression
genetic transformation
genetics
kinetics
metabolism
plasmid
Bacterial Proteins
Escherichia coli
Gene Expression
Genes, Bacterial
Kanamycin Kinase
Kanamycin Resistance
Kinetics
Phosphotransferases (Alcohol Group Acceptor)
Plasmids
Polyamines
Putrescine
RNA, Bacterial
RNA, Messenger
Transformation, Genetic
spellingShingle bacterial protein
bacterial RNA
kanamycin kinase
messenger RNA
phosphotransferase
polyamine
putrescine
antibiotic resistance
article
bacterial gene
biosynthesis
drug effect
enzymology
Escherichia coli
gene expression
genetic transformation
genetics
kinetics
metabolism
plasmid
Bacterial Proteins
Escherichia coli
Gene Expression
Genes, Bacterial
Kanamycin Kinase
Kanamycin Resistance
Kinetics
Phosphotransferases (Alcohol Group Acceptor)
Plasmids
Polyamines
Putrescine
RNA, Bacterial
RNA, Messenger
Transformation, Genetic
Nastri, Horacio Gabriel
Algranati, Israel David
Effect of polyamines on plasmid-mediated kanamycin resistance and kanamycin phosphotransferase gene expression in Escherichia coli.
topic_facet bacterial protein
bacterial RNA
kanamycin kinase
messenger RNA
phosphotransferase
polyamine
putrescine
antibiotic resistance
article
bacterial gene
biosynthesis
drug effect
enzymology
Escherichia coli
gene expression
genetic transformation
genetics
kinetics
metabolism
plasmid
Bacterial Proteins
Escherichia coli
Gene Expression
Genes, Bacterial
Kanamycin Kinase
Kanamycin Resistance
Kinetics
Phosphotransferases (Alcohol Group Acceptor)
Plasmids
Polyamines
Putrescine
RNA, Bacterial
RNA, Messenger
Transformation, Genetic
description The emergence of kanamycin resistance in a polyamine-deficient mutant of E. coli transformed with a plasmid encoding the kanamycin phosphotransferase gene has been studied. The initial inhibition of growth and protein synthesis caused by the addition of the antibiotic could be reversed earlier in polyamine-supplemented bacteria than in those depleted of the organic bases. Concomitantly, we have observed that the increase of kanamycin phosphotransferase activity evoking the antibiotic resistance was higher in bacteria cultivated in the presence of putrescine. This result seems to depend exclusively on the enhanced capacity of the translation process in bacteria grown with polyamines since the transcription of phosphotransferase gene was higher in cells subjected to polyamine starvation.
author Nastri, Horacio Gabriel
Algranati, Israel David
author_facet Nastri, Horacio Gabriel
Algranati, Israel David
author_sort Nastri, Horacio Gabriel
title Effect of polyamines on plasmid-mediated kanamycin resistance and kanamycin phosphotransferase gene expression in Escherichia coli.
title_short Effect of polyamines on plasmid-mediated kanamycin resistance and kanamycin phosphotransferase gene expression in Escherichia coli.
title_full Effect of polyamines on plasmid-mediated kanamycin resistance and kanamycin phosphotransferase gene expression in Escherichia coli.
title_fullStr Effect of polyamines on plasmid-mediated kanamycin resistance and kanamycin phosphotransferase gene expression in Escherichia coli.
title_full_unstemmed Effect of polyamines on plasmid-mediated kanamycin resistance and kanamycin phosphotransferase gene expression in Escherichia coli.
title_sort effect of polyamines on plasmid-mediated kanamycin resistance and kanamycin phosphotransferase gene expression in escherichia coli.
publishDate 1996
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01455680_v42_n5_p711_Nastri
http://hdl.handle.net/20.500.12110/paper_01455680_v42_n5_p711_Nastri
work_keys_str_mv AT nastrihoraciogabriel effectofpolyaminesonplasmidmediatedkanamycinresistanceandkanamycinphosphotransferasegeneexpressioninescherichiacoli
AT algranatiisraeldavid effectofpolyaminesonplasmidmediatedkanamycinresistanceandkanamycinphosphotransferasegeneexpressioninescherichiacoli
_version_ 1768543554702934016

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