Regulation of putrescine uptake in Leishmania mexicana promastigotes.
Putrescine uptake of Leishmania mexicana promastigotes is tightly regulated by polyamine intracellular concentrations. This uptake, markedly stimulated after parasite treatment with alpha-difluoromethylornithine (DFMO) for 48 to 72 hrs., was strongly repressed by exposure of Leishmania cultures to e...
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1994
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Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01455680_v40_n7_p907_Gonzalez http://hdl.handle.net/20.500.12110/paper_01455680_v40_n7_p907_Gonzalez |
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paper:paper_01455680_v40_n7_p907_Gonzalez2023-06-08T15:12:19Z Regulation of putrescine uptake in Leishmania mexicana promastigotes. González, Nélida Susana Algranati, Israel David cycloheximide eflornithine polyamine putrescine active transport animal article drug effect feedback system growth, development and aging kinetics Leishmania mexicana metabolism Animals Biological Transport, Active Cycloheximide Eflornithine Feedback Kinetics Leishmania mexicana Polyamines Putrescine Putrescine uptake of Leishmania mexicana promastigotes is tightly regulated by polyamine intracellular concentrations. This uptake, markedly stimulated after parasite treatment with alpha-difluoromethylornithine (DFMO) for 48 to 72 hrs., was strongly repressed by exposure of Leishmania cultures to exogenous putrescine or its derivative 1,4-dimethylputrescine. In contrast, spermidine, spermine, diaminopropane and cadaverine were unable to decrease putrescine transport. Both, the uptake induction as well as its specific feedback repression by increased levels of endogenous putrescine requires protein synthesis since they were abolished after addition of cycloheximide for several hours. Our results seem to indicate that putrescine transporter is a stable and specific protein which can be reversibly inactivated by a relatively unstable repressor. Fil:Gonzalez, N.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Algranati, I.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1994 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01455680_v40_n7_p907_Gonzalez http://hdl.handle.net/20.500.12110/paper_01455680_v40_n7_p907_Gonzalez |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
cycloheximide eflornithine polyamine putrescine active transport animal article drug effect feedback system growth, development and aging kinetics Leishmania mexicana metabolism Animals Biological Transport, Active Cycloheximide Eflornithine Feedback Kinetics Leishmania mexicana Polyamines Putrescine |
spellingShingle |
cycloheximide eflornithine polyamine putrescine active transport animal article drug effect feedback system growth, development and aging kinetics Leishmania mexicana metabolism Animals Biological Transport, Active Cycloheximide Eflornithine Feedback Kinetics Leishmania mexicana Polyamines Putrescine González, Nélida Susana Algranati, Israel David Regulation of putrescine uptake in Leishmania mexicana promastigotes. |
topic_facet |
cycloheximide eflornithine polyamine putrescine active transport animal article drug effect feedback system growth, development and aging kinetics Leishmania mexicana metabolism Animals Biological Transport, Active Cycloheximide Eflornithine Feedback Kinetics Leishmania mexicana Polyamines Putrescine |
description |
Putrescine uptake of Leishmania mexicana promastigotes is tightly regulated by polyamine intracellular concentrations. This uptake, markedly stimulated after parasite treatment with alpha-difluoromethylornithine (DFMO) for 48 to 72 hrs., was strongly repressed by exposure of Leishmania cultures to exogenous putrescine or its derivative 1,4-dimethylputrescine. In contrast, spermidine, spermine, diaminopropane and cadaverine were unable to decrease putrescine transport. Both, the uptake induction as well as its specific feedback repression by increased levels of endogenous putrescine requires protein synthesis since they were abolished after addition of cycloheximide for several hours. Our results seem to indicate that putrescine transporter is a stable and specific protein which can be reversibly inactivated by a relatively unstable repressor. |
author |
González, Nélida Susana Algranati, Israel David |
author_facet |
González, Nélida Susana Algranati, Israel David |
author_sort |
González, Nélida Susana |
title |
Regulation of putrescine uptake in Leishmania mexicana promastigotes. |
title_short |
Regulation of putrescine uptake in Leishmania mexicana promastigotes. |
title_full |
Regulation of putrescine uptake in Leishmania mexicana promastigotes. |
title_fullStr |
Regulation of putrescine uptake in Leishmania mexicana promastigotes. |
title_full_unstemmed |
Regulation of putrescine uptake in Leishmania mexicana promastigotes. |
title_sort |
regulation of putrescine uptake in leishmania mexicana promastigotes. |
publishDate |
1994 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01455680_v40_n7_p907_Gonzalez http://hdl.handle.net/20.500.12110/paper_01455680_v40_n7_p907_Gonzalez |
work_keys_str_mv |
AT gonzaleznelidasusana regulationofputrescineuptakeinleishmaniamexicanapromastigotes AT algranatiisraeldavid regulationofputrescineuptakeinleishmaniamexicanapromastigotes |
_version_ |
1768544725461106688 |