The in vitro biosynthesis of functional nodulation factors (Nod Rm) produced by Rhizobium meliloti 1021.
Rhizobium meliloti associates symbiotically with alfalfa by forming root nodules in which the bacteria reduce atmospheric N2 into products useful to both organisms. Nod factors are signal molecules, lipooligosaccharides, produced by the bacteria that trigger nodule formation in the plant host. Nod R...
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1994
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Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01455680_v40_n7_p1029_Semino http://hdl.handle.net/20.500.12110/paper_01455680_v40_n7_p1029_Semino |
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paper:paper_01455680_v40_n7_p1029_Semino2023-06-08T15:12:19Z The in vitro biosynthesis of functional nodulation factors (Nod Rm) produced by Rhizobium meliloti 1021. Semino, Carlos Eduardo Dankert, Marcelo Alberto lipid linked oligosaccharides lipid-linked oligosaccharides lipopolysaccharide alfalfa article bacterial gene biosynthesis carbohydrate analysis chemical structure chemistry genetics metabolism microbiology molecular genetics Sinorhizobium meliloti Carbohydrate Sequence Genes, Bacterial Lipopolysaccharides Medicago sativa Molecular Sequence Data Molecular Structure Sinorhizobium meliloti Rhizobium meliloti associates symbiotically with alfalfa by forming root nodules in which the bacteria reduce atmospheric N2 into products useful to both organisms. Nod factors are signal molecules, lipooligosaccharides, produced by the bacteria that trigger nodule formation in the plant host. Nod Rm-1 consists of a beta-1,4-N-acetyl glucosamine tetrasaccharide from which the N-acetyl group at the non reducing end is replaced by a fatty acid and the N-acetyl glucosamine at the reducing end is sulfated at position 6. By in vitro incubation of electroporated cells in the presence of [35S]PAPS or UDP-[14C]GlcNAc a labelled compound has been obtained with the properties of the in vivo produced Nod Rm-1 factor, as judged by HPLC, TLC and HPTLC techniques. The [14C]GlcNAc labelled compound has also hair root deformation activity on alfalfa plantlets indicating that a functional Nod Rm-1 factor has been synthesized in vitro. Fil:Semino, C.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Dankert, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1994 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01455680_v40_n7_p1029_Semino http://hdl.handle.net/20.500.12110/paper_01455680_v40_n7_p1029_Semino |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
lipid linked oligosaccharides lipid-linked oligosaccharides lipopolysaccharide alfalfa article bacterial gene biosynthesis carbohydrate analysis chemical structure chemistry genetics metabolism microbiology molecular genetics Sinorhizobium meliloti Carbohydrate Sequence Genes, Bacterial Lipopolysaccharides Medicago sativa Molecular Sequence Data Molecular Structure Sinorhizobium meliloti |
spellingShingle |
lipid linked oligosaccharides lipid-linked oligosaccharides lipopolysaccharide alfalfa article bacterial gene biosynthesis carbohydrate analysis chemical structure chemistry genetics metabolism microbiology molecular genetics Sinorhizobium meliloti Carbohydrate Sequence Genes, Bacterial Lipopolysaccharides Medicago sativa Molecular Sequence Data Molecular Structure Sinorhizobium meliloti Semino, Carlos Eduardo Dankert, Marcelo Alberto The in vitro biosynthesis of functional nodulation factors (Nod Rm) produced by Rhizobium meliloti 1021. |
topic_facet |
lipid linked oligosaccharides lipid-linked oligosaccharides lipopolysaccharide alfalfa article bacterial gene biosynthesis carbohydrate analysis chemical structure chemistry genetics metabolism microbiology molecular genetics Sinorhizobium meliloti Carbohydrate Sequence Genes, Bacterial Lipopolysaccharides Medicago sativa Molecular Sequence Data Molecular Structure Sinorhizobium meliloti |
description |
Rhizobium meliloti associates symbiotically with alfalfa by forming root nodules in which the bacteria reduce atmospheric N2 into products useful to both organisms. Nod factors are signal molecules, lipooligosaccharides, produced by the bacteria that trigger nodule formation in the plant host. Nod Rm-1 consists of a beta-1,4-N-acetyl glucosamine tetrasaccharide from which the N-acetyl group at the non reducing end is replaced by a fatty acid and the N-acetyl glucosamine at the reducing end is sulfated at position 6. By in vitro incubation of electroporated cells in the presence of [35S]PAPS or UDP-[14C]GlcNAc a labelled compound has been obtained with the properties of the in vivo produced Nod Rm-1 factor, as judged by HPLC, TLC and HPTLC techniques. The [14C]GlcNAc labelled compound has also hair root deformation activity on alfalfa plantlets indicating that a functional Nod Rm-1 factor has been synthesized in vitro. |
author |
Semino, Carlos Eduardo Dankert, Marcelo Alberto |
author_facet |
Semino, Carlos Eduardo Dankert, Marcelo Alberto |
author_sort |
Semino, Carlos Eduardo |
title |
The in vitro biosynthesis of functional nodulation factors (Nod Rm) produced by Rhizobium meliloti 1021. |
title_short |
The in vitro biosynthesis of functional nodulation factors (Nod Rm) produced by Rhizobium meliloti 1021. |
title_full |
The in vitro biosynthesis of functional nodulation factors (Nod Rm) produced by Rhizobium meliloti 1021. |
title_fullStr |
The in vitro biosynthesis of functional nodulation factors (Nod Rm) produced by Rhizobium meliloti 1021. |
title_full_unstemmed |
The in vitro biosynthesis of functional nodulation factors (Nod Rm) produced by Rhizobium meliloti 1021. |
title_sort |
in vitro biosynthesis of functional nodulation factors (nod rm) produced by rhizobium meliloti 1021. |
publishDate |
1994 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01455680_v40_n7_p1029_Semino http://hdl.handle.net/20.500.12110/paper_01455680_v40_n7_p1029_Semino |
work_keys_str_mv |
AT seminocarloseduardo theinvitrobiosynthesisoffunctionalnodulationfactorsnodrmproducedbyrhizobiummeliloti1021 AT dankertmarceloalberto theinvitrobiosynthesisoffunctionalnodulationfactorsnodrmproducedbyrhizobiummeliloti1021 AT seminocarloseduardo invitrobiosynthesisoffunctionalnodulationfactorsnodrmproducedbyrhizobiummeliloti1021 AT dankertmarceloalberto invitrobiosynthesisoffunctionalnodulationfactorsnodrmproducedbyrhizobiummeliloti1021 |
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1768543272274231296 |