The system of sulfated galactans from the red seaweed Gymnogongrus torulosus (Phyllophoraceae, Rhodophyta): Location and structural analysis
Sulfated polysaccharides were localized in the cuticle, cortex and medulla of the gametophyte thallus, being more concentrated in the intercellular matrix than in the cell walls. During the water extraction sequence, a small percentage of galactan sulfates (5.1% of dry seaweed) with average low Mr (...
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2008
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01448617_v73_n4_p594_Estevez http://hdl.handle.net/20.500.12110/paper_01448617_v73_n4_p594_Estevez |
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paper:paper_01448617_v73_n4_p594_Estevez2023-06-08T15:12:11Z The system of sulfated galactans from the red seaweed Gymnogongrus torulosus (Phyllophoraceae, Rhodophyta): Location and structural analysis Estevez, Jose Manuel Ciancia, Marina Cerezo, Alberto Saúl Agarans Carrageenans Cell wall Gymnogongrus torulosus (Phyllophoraceae) l-Galactose Red seaweed Sulfated galactans Algae Cell membranes Dialysis Extraction Molecular structure Tissue Agarans Carrageenans Gymnogongrus torulosus (Phyllophoraceae) l-Galactose Red seaweed Sulfated galactans Polysaccharides Gymnogongrus torulosus Phyllophoraceae Rhodophyta Sulfated polysaccharides were localized in the cuticle, cortex and medulla of the gametophyte thallus, being more concentrated in the intercellular matrix than in the cell walls. During the water extraction sequence, a small percentage of galactan sulfates (5.1% of dry seaweed) with average low Mr (6-11.4 kDa) were extracted at room temperature without disturbing the cellular arrangement, while sulfated galactans of average medium Mr (18-45 kDa) were obtained by further hot-water extractions (52.4% of dry seaweed), with diorganization of the tissue. The residue (40.0% of dry seaweed) still contained carrageenan-type (major) and agaran-type (minor) galactans. Part of these galactans was extracted with 8.4% LiCl solution in DMSO, from which "pure" κ/ι-carrageenans were isolated. Carrageenans and agarans were extracted in a ratio 1:0.5, showing the highest amount of agaran-structures for a carrageenophyte. The galactans comprise alternating 4-sulfated (major) and non-sulfated (minor) 3-linked β-d-galactopyranose units, and 4-linked α-galactopyranose units with the following substitutions: (i) non-sulfated and 2-sulfated 3,6-anhydro-α-d-galactopyranose residues in the carrageenan-structures, which belong to the κ-family (κ/ι-carrageenans); (ii) 3-sulfated α-l-galactopyranose units and 2-sulfated 3,6-anhydro-α-l-galactopyranose residues in the agaran-structures. Alkaline treatment and alkaline dialysis of the main extracts gave "pure" κ/ι-carrageenans, showing that carrageenan molecules are extracted together with low Mr agarans or agaran-dl-hybrids. © 2008 Elsevier Ltd. All rights reserved. Fil:Estevez, J.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ciancia, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cerezo, A.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2008 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01448617_v73_n4_p594_Estevez http://hdl.handle.net/20.500.12110/paper_01448617_v73_n4_p594_Estevez |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Agarans Carrageenans Cell wall Gymnogongrus torulosus (Phyllophoraceae) l-Galactose Red seaweed Sulfated galactans Algae Cell membranes Dialysis Extraction Molecular structure Tissue Agarans Carrageenans Gymnogongrus torulosus (Phyllophoraceae) l-Galactose Red seaweed Sulfated galactans Polysaccharides Gymnogongrus torulosus Phyllophoraceae Rhodophyta |
| spellingShingle |
Agarans Carrageenans Cell wall Gymnogongrus torulosus (Phyllophoraceae) l-Galactose Red seaweed Sulfated galactans Algae Cell membranes Dialysis Extraction Molecular structure Tissue Agarans Carrageenans Gymnogongrus torulosus (Phyllophoraceae) l-Galactose Red seaweed Sulfated galactans Polysaccharides Gymnogongrus torulosus Phyllophoraceae Rhodophyta Estevez, Jose Manuel Ciancia, Marina Cerezo, Alberto Saúl The system of sulfated galactans from the red seaweed Gymnogongrus torulosus (Phyllophoraceae, Rhodophyta): Location and structural analysis |
| topic_facet |
Agarans Carrageenans Cell wall Gymnogongrus torulosus (Phyllophoraceae) l-Galactose Red seaweed Sulfated galactans Algae Cell membranes Dialysis Extraction Molecular structure Tissue Agarans Carrageenans Gymnogongrus torulosus (Phyllophoraceae) l-Galactose Red seaweed Sulfated galactans Polysaccharides Gymnogongrus torulosus Phyllophoraceae Rhodophyta |
| description |
Sulfated polysaccharides were localized in the cuticle, cortex and medulla of the gametophyte thallus, being more concentrated in the intercellular matrix than in the cell walls. During the water extraction sequence, a small percentage of galactan sulfates (5.1% of dry seaweed) with average low Mr (6-11.4 kDa) were extracted at room temperature without disturbing the cellular arrangement, while sulfated galactans of average medium Mr (18-45 kDa) were obtained by further hot-water extractions (52.4% of dry seaweed), with diorganization of the tissue. The residue (40.0% of dry seaweed) still contained carrageenan-type (major) and agaran-type (minor) galactans. Part of these galactans was extracted with 8.4% LiCl solution in DMSO, from which "pure" κ/ι-carrageenans were isolated. Carrageenans and agarans were extracted in a ratio 1:0.5, showing the highest amount of agaran-structures for a carrageenophyte. The galactans comprise alternating 4-sulfated (major) and non-sulfated (minor) 3-linked β-d-galactopyranose units, and 4-linked α-galactopyranose units with the following substitutions: (i) non-sulfated and 2-sulfated 3,6-anhydro-α-d-galactopyranose residues in the carrageenan-structures, which belong to the κ-family (κ/ι-carrageenans); (ii) 3-sulfated α-l-galactopyranose units and 2-sulfated 3,6-anhydro-α-l-galactopyranose residues in the agaran-structures. Alkaline treatment and alkaline dialysis of the main extracts gave "pure" κ/ι-carrageenans, showing that carrageenan molecules are extracted together with low Mr agarans or agaran-dl-hybrids. © 2008 Elsevier Ltd. All rights reserved. |
| author |
Estevez, Jose Manuel Ciancia, Marina Cerezo, Alberto Saúl |
| author_facet |
Estevez, Jose Manuel Ciancia, Marina Cerezo, Alberto Saúl |
| author_sort |
Estevez, Jose Manuel |
| title |
The system of sulfated galactans from the red seaweed Gymnogongrus torulosus (Phyllophoraceae, Rhodophyta): Location and structural analysis |
| title_short |
The system of sulfated galactans from the red seaweed Gymnogongrus torulosus (Phyllophoraceae, Rhodophyta): Location and structural analysis |
| title_full |
The system of sulfated galactans from the red seaweed Gymnogongrus torulosus (Phyllophoraceae, Rhodophyta): Location and structural analysis |
| title_fullStr |
The system of sulfated galactans from the red seaweed Gymnogongrus torulosus (Phyllophoraceae, Rhodophyta): Location and structural analysis |
| title_full_unstemmed |
The system of sulfated galactans from the red seaweed Gymnogongrus torulosus (Phyllophoraceae, Rhodophyta): Location and structural analysis |
| title_sort |
system of sulfated galactans from the red seaweed gymnogongrus torulosus (phyllophoraceae, rhodophyta): location and structural analysis |
| publishDate |
2008 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01448617_v73_n4_p594_Estevez http://hdl.handle.net/20.500.12110/paper_01448617_v73_n4_p594_Estevez |
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| _version_ |
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