Phototriggered fibril-like environments arbitrate cell escapes and migration from endothelial monolayers
Cell detachment and migration from the endothelium occurs during vasculogenesis and also in pathological states. Here, we use a novel approach to trigger single cell release from an endothelial monolayer by in-situ opening of adhesive, fibril-like environment using light-responsive ligands and scann...
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2016
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01429612_v82_n_p113_Salierno http://hdl.handle.net/20.500.12110/paper_01429612_v82_n_p113_Salierno |
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paper:paper_01429612_v82_n_p113_Salierno2023-06-08T15:11:42Z Phototriggered fibril-like environments arbitrate cell escapes and migration from endothelial monolayers Cell migration Cyclic RGD Endothelial transition Photoactive materials Cytology Endothelial cells Ligands Monolayers Adhesive ligands Cell detachment Cell migration Cyclic RGD Endothelial transition Nuclear deformations Photoactive materials Scanning lasers Cells adhesive ligand blebbistatin ligand myosin myosin adenosine triphosphatase unclassified drug vasculotropin arginyl-glycyl-aspartic acid oligopeptide Article cell adhesion cell body cell density cell detachment cell fate cell interaction cell migration cell motion cell nucleus cell polarity cell proliferation cell shape controlled study endothelium cell focal adhesion lamellipodium light muscle contractility photoactivation priority journal adhesion cell culture cell motion chemistry cytology endothelium endothelium cell extracellular matrix human metabolism physiology radiation response tumor microenvironment Adhesiveness Cell Adhesion Cell Movement Cells, Cultured Cellular Microenvironment Endothelial Cells Endothelium Extracellular Matrix Humans Light Oligopeptides Cell detachment and migration from the endothelium occurs during vasculogenesis and also in pathological states. Here, we use a novel approach to trigger single cell release from an endothelial monolayer by in-situ opening of adhesive, fibril-like environment using light-responsive ligands and scanning lasers. Cell escapes from the monolayer were observed on the fibril-like adhesive tracks with 3-15 μm width. The frequency of endothelial cell escapes increased monotonically with the fibril width and with the density of the light-activated adhesive ligand. Interestingly, treatment with VEGF induced cohesiveness within the cell layer, preventing cell leaks. When migrating through the tracks, cells presented body lateral reduction and nuclear deformation imposed by the line width and dependent on myosin contractility. Cell migration mode changed from mesenchymal to amoeboid-like when the adhesive tracks narrowed (≤5 μm). Moreover, cell nucleus was shrunk showing packed DNA on lines narrower than the nuclear dimensions in a mechanisms intimately associated with the stress fibers. This platform allows the detailed study of escapes and migratory transitions of cohesive cells, which are relevant processes in development and during diseases such as organ fibrosis and carcinomas. © 2015 Elsevier Ltd. 2016 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01429612_v82_n_p113_Salierno http://hdl.handle.net/20.500.12110/paper_01429612_v82_n_p113_Salierno |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Cell migration Cyclic RGD Endothelial transition Photoactive materials Cytology Endothelial cells Ligands Monolayers Adhesive ligands Cell detachment Cell migration Cyclic RGD Endothelial transition Nuclear deformations Photoactive materials Scanning lasers Cells adhesive ligand blebbistatin ligand myosin myosin adenosine triphosphatase unclassified drug vasculotropin arginyl-glycyl-aspartic acid oligopeptide Article cell adhesion cell body cell density cell detachment cell fate cell interaction cell migration cell motion cell nucleus cell polarity cell proliferation cell shape controlled study endothelium cell focal adhesion lamellipodium light muscle contractility photoactivation priority journal adhesion cell culture cell motion chemistry cytology endothelium endothelium cell extracellular matrix human metabolism physiology radiation response tumor microenvironment Adhesiveness Cell Adhesion Cell Movement Cells, Cultured Cellular Microenvironment Endothelial Cells Endothelium Extracellular Matrix Humans Light Oligopeptides |
| spellingShingle |
Cell migration Cyclic RGD Endothelial transition Photoactive materials Cytology Endothelial cells Ligands Monolayers Adhesive ligands Cell detachment Cell migration Cyclic RGD Endothelial transition Nuclear deformations Photoactive materials Scanning lasers Cells adhesive ligand blebbistatin ligand myosin myosin adenosine triphosphatase unclassified drug vasculotropin arginyl-glycyl-aspartic acid oligopeptide Article cell adhesion cell body cell density cell detachment cell fate cell interaction cell migration cell motion cell nucleus cell polarity cell proliferation cell shape controlled study endothelium cell focal adhesion lamellipodium light muscle contractility photoactivation priority journal adhesion cell culture cell motion chemistry cytology endothelium endothelium cell extracellular matrix human metabolism physiology radiation response tumor microenvironment Adhesiveness Cell Adhesion Cell Movement Cells, Cultured Cellular Microenvironment Endothelial Cells Endothelium Extracellular Matrix Humans Light Oligopeptides Phototriggered fibril-like environments arbitrate cell escapes and migration from endothelial monolayers |
| topic_facet |
Cell migration Cyclic RGD Endothelial transition Photoactive materials Cytology Endothelial cells Ligands Monolayers Adhesive ligands Cell detachment Cell migration Cyclic RGD Endothelial transition Nuclear deformations Photoactive materials Scanning lasers Cells adhesive ligand blebbistatin ligand myosin myosin adenosine triphosphatase unclassified drug vasculotropin arginyl-glycyl-aspartic acid oligopeptide Article cell adhesion cell body cell density cell detachment cell fate cell interaction cell migration cell motion cell nucleus cell polarity cell proliferation cell shape controlled study endothelium cell focal adhesion lamellipodium light muscle contractility photoactivation priority journal adhesion cell culture cell motion chemistry cytology endothelium endothelium cell extracellular matrix human metabolism physiology radiation response tumor microenvironment Adhesiveness Cell Adhesion Cell Movement Cells, Cultured Cellular Microenvironment Endothelial Cells Endothelium Extracellular Matrix Humans Light Oligopeptides |
| description |
Cell detachment and migration from the endothelium occurs during vasculogenesis and also in pathological states. Here, we use a novel approach to trigger single cell release from an endothelial monolayer by in-situ opening of adhesive, fibril-like environment using light-responsive ligands and scanning lasers. Cell escapes from the monolayer were observed on the fibril-like adhesive tracks with 3-15 μm width. The frequency of endothelial cell escapes increased monotonically with the fibril width and with the density of the light-activated adhesive ligand. Interestingly, treatment with VEGF induced cohesiveness within the cell layer, preventing cell leaks. When migrating through the tracks, cells presented body lateral reduction and nuclear deformation imposed by the line width and dependent on myosin contractility. Cell migration mode changed from mesenchymal to amoeboid-like when the adhesive tracks narrowed (≤5 μm). Moreover, cell nucleus was shrunk showing packed DNA on lines narrower than the nuclear dimensions in a mechanisms intimately associated with the stress fibers. This platform allows the detailed study of escapes and migratory transitions of cohesive cells, which are relevant processes in development and during diseases such as organ fibrosis and carcinomas. © 2015 Elsevier Ltd. |
| title |
Phototriggered fibril-like environments arbitrate cell escapes and migration from endothelial monolayers |
| title_short |
Phototriggered fibril-like environments arbitrate cell escapes and migration from endothelial monolayers |
| title_full |
Phototriggered fibril-like environments arbitrate cell escapes and migration from endothelial monolayers |
| title_fullStr |
Phototriggered fibril-like environments arbitrate cell escapes and migration from endothelial monolayers |
| title_full_unstemmed |
Phototriggered fibril-like environments arbitrate cell escapes and migration from endothelial monolayers |
| title_sort |
phototriggered fibril-like environments arbitrate cell escapes and migration from endothelial monolayers |
| publishDate |
2016 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01429612_v82_n_p113_Salierno http://hdl.handle.net/20.500.12110/paper_01429612_v82_n_p113_Salierno |
| _version_ |
1768545412720885760 |