S-layer proteins from Lactobacillus sp. inhibit bacterial infection by blockage of DC-SIGN cell receptor
Many species of Lactobacillus sp. possess Surface(s) layer proteins in their envelope. Among other important characteristics S-layer from Lactobacillus acidophilus binds to the cellular receptor DC-SIGN (Dendritic Cell-Specific Intercellular adhesion molecule-3-Grabbing Non-integrin; CD209), which i...
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2016
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01418130_v92_n_p998_PradoAcosta http://hdl.handle.net/20.500.12110/paper_01418130_v92_n_p998_PradoAcosta |
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paper:paper_01418130_v92_n_p998_PradoAcosta2023-06-08T15:11:26Z S-layer proteins from Lactobacillus sp. inhibit bacterial infection by blockage of DC-SIGN cell receptor Prado Acosta, Mariano Ruzal, Sandra Mónica Cordo, Sandra M. DC-SIGN Lactobacillus S-layer bacterial protein CD209 antigen s layer protein unclassified drug antiinfective agent cell adhesion molecule cell surface receptor DC-specific ICAM-3 grabbing nonintegrin lectin membrane protein S-layer proteins Article bacterial strain bacterial viability bacterium adherence controlled study enzyme inhibition human human cell in vivo study Lactobacillus Lactobacillus acidophilus Lactobacillus brevis Lactobacillus helveticus Lactobacillus kefiri nonhuman protein function protein glycosylation protein protein interaction 3T3 cell line animal Bacterial Infections chemistry drug effects glycosylation metabolism mouse 3T3 Cells Animals Anti-Bacterial Agents Bacterial Adhesion Bacterial Infections Cell Adhesion Molecules Glycosylation Humans Lactobacillus Lectins, C-Type Membrane Glycoproteins Mice Receptors, Cell Surface Many species of Lactobacillus sp. possess Surface(s) layer proteins in their envelope. Among other important characteristics S-layer from Lactobacillus acidophilus binds to the cellular receptor DC-SIGN (Dendritic Cell-Specific Intercellular adhesion molecule-3-Grabbing Non-integrin; CD209), which is involved in adhesion and infection of several families of bacteria. In this report we investigate the activity of new S-layer proteins from the Lactobacillus family (Lactobacillus acidophilus, Lactobacillus brevis, Lactobacillus helveticus and Lactobacillus kefiri) over the infection of representative microorganisms important to human health. After the treatment of DC-SIGN expressing cells with these proteins, we were able to diminish bacterial infection by up to 79% in both gram negative and mycobacterial models. We discovered that pre-treatment of the bacteria with S-layers from Lactobacillus acidophilus and Lactobacillus brevis reduced bacteria viability but also prevent infection by the pathogenic bacteria. We also proved the importance of the glycosylation of the S-layer from Lactobacillus kefiri in the binding to the receptor and thus inhibition of infection. This novel characteristic of the S-layers proteins may contribute to the already reported pathogen exclusion activity for these Lactobacillus probiotic strains; and might be also considered as a novel enzymatic antimicrobial agents to inhibit bacterial infection and entry to host cells. © 2016 Elsevier B.V. Fil:Prado Acosta, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ruzal, S.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cordo, S.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2016 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01418130_v92_n_p998_PradoAcosta http://hdl.handle.net/20.500.12110/paper_01418130_v92_n_p998_PradoAcosta |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
DC-SIGN Lactobacillus S-layer bacterial protein CD209 antigen s layer protein unclassified drug antiinfective agent cell adhesion molecule cell surface receptor DC-specific ICAM-3 grabbing nonintegrin lectin membrane protein S-layer proteins Article bacterial strain bacterial viability bacterium adherence controlled study enzyme inhibition human human cell in vivo study Lactobacillus Lactobacillus acidophilus Lactobacillus brevis Lactobacillus helveticus Lactobacillus kefiri nonhuman protein function protein glycosylation protein protein interaction 3T3 cell line animal Bacterial Infections chemistry drug effects glycosylation metabolism mouse 3T3 Cells Animals Anti-Bacterial Agents Bacterial Adhesion Bacterial Infections Cell Adhesion Molecules Glycosylation Humans Lactobacillus Lectins, C-Type Membrane Glycoproteins Mice Receptors, Cell Surface |
| spellingShingle |
DC-SIGN Lactobacillus S-layer bacterial protein CD209 antigen s layer protein unclassified drug antiinfective agent cell adhesion molecule cell surface receptor DC-specific ICAM-3 grabbing nonintegrin lectin membrane protein S-layer proteins Article bacterial strain bacterial viability bacterium adherence controlled study enzyme inhibition human human cell in vivo study Lactobacillus Lactobacillus acidophilus Lactobacillus brevis Lactobacillus helveticus Lactobacillus kefiri nonhuman protein function protein glycosylation protein protein interaction 3T3 cell line animal Bacterial Infections chemistry drug effects glycosylation metabolism mouse 3T3 Cells Animals Anti-Bacterial Agents Bacterial Adhesion Bacterial Infections Cell Adhesion Molecules Glycosylation Humans Lactobacillus Lectins, C-Type Membrane Glycoproteins Mice Receptors, Cell Surface Prado Acosta, Mariano Ruzal, Sandra Mónica Cordo, Sandra M. S-layer proteins from Lactobacillus sp. inhibit bacterial infection by blockage of DC-SIGN cell receptor |
| topic_facet |
DC-SIGN Lactobacillus S-layer bacterial protein CD209 antigen s layer protein unclassified drug antiinfective agent cell adhesion molecule cell surface receptor DC-specific ICAM-3 grabbing nonintegrin lectin membrane protein S-layer proteins Article bacterial strain bacterial viability bacterium adherence controlled study enzyme inhibition human human cell in vivo study Lactobacillus Lactobacillus acidophilus Lactobacillus brevis Lactobacillus helveticus Lactobacillus kefiri nonhuman protein function protein glycosylation protein protein interaction 3T3 cell line animal Bacterial Infections chemistry drug effects glycosylation metabolism mouse 3T3 Cells Animals Anti-Bacterial Agents Bacterial Adhesion Bacterial Infections Cell Adhesion Molecules Glycosylation Humans Lactobacillus Lectins, C-Type Membrane Glycoproteins Mice Receptors, Cell Surface |
| description |
Many species of Lactobacillus sp. possess Surface(s) layer proteins in their envelope. Among other important characteristics S-layer from Lactobacillus acidophilus binds to the cellular receptor DC-SIGN (Dendritic Cell-Specific Intercellular adhesion molecule-3-Grabbing Non-integrin; CD209), which is involved in adhesion and infection of several families of bacteria. In this report we investigate the activity of new S-layer proteins from the Lactobacillus family (Lactobacillus acidophilus, Lactobacillus brevis, Lactobacillus helveticus and Lactobacillus kefiri) over the infection of representative microorganisms important to human health. After the treatment of DC-SIGN expressing cells with these proteins, we were able to diminish bacterial infection by up to 79% in both gram negative and mycobacterial models. We discovered that pre-treatment of the bacteria with S-layers from Lactobacillus acidophilus and Lactobacillus brevis reduced bacteria viability but also prevent infection by the pathogenic bacteria. We also proved the importance of the glycosylation of the S-layer from Lactobacillus kefiri in the binding to the receptor and thus inhibition of infection. This novel characteristic of the S-layers proteins may contribute to the already reported pathogen exclusion activity for these Lactobacillus probiotic strains; and might be also considered as a novel enzymatic antimicrobial agents to inhibit bacterial infection and entry to host cells. © 2016 Elsevier B.V. |
| author |
Prado Acosta, Mariano Ruzal, Sandra Mónica Cordo, Sandra M. |
| author_facet |
Prado Acosta, Mariano Ruzal, Sandra Mónica Cordo, Sandra M. |
| author_sort |
Prado Acosta, Mariano |
| title |
S-layer proteins from Lactobacillus sp. inhibit bacterial infection by blockage of DC-SIGN cell receptor |
| title_short |
S-layer proteins from Lactobacillus sp. inhibit bacterial infection by blockage of DC-SIGN cell receptor |
| title_full |
S-layer proteins from Lactobacillus sp. inhibit bacterial infection by blockage of DC-SIGN cell receptor |
| title_fullStr |
S-layer proteins from Lactobacillus sp. inhibit bacterial infection by blockage of DC-SIGN cell receptor |
| title_full_unstemmed |
S-layer proteins from Lactobacillus sp. inhibit bacterial infection by blockage of DC-SIGN cell receptor |
| title_sort |
s-layer proteins from lactobacillus sp. inhibit bacterial infection by blockage of dc-sign cell receptor |
| publishDate |
2016 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01418130_v92_n_p998_PradoAcosta http://hdl.handle.net/20.500.12110/paper_01418130_v92_n_p998_PradoAcosta |
| work_keys_str_mv |
AT pradoacostamariano slayerproteinsfromlactobacillusspinhibitbacterialinfectionbyblockageofdcsigncellreceptor AT ruzalsandramonica slayerproteinsfromlactobacillusspinhibitbacterialinfectionbyblockageofdcsigncellreceptor AT cordosandram slayerproteinsfromlactobacillusspinhibitbacterialinfectionbyblockageofdcsigncellreceptor |
| _version_ |
1768544404185808896 |