Tn 1000 (γδ) insertions stabilize pBR322-derived recombinant plasmids in Escherichia coli

Tn1000 (or 'γδ') insertions in a pBR322-recombinant plasmid carrying the threonine operon (pBE) were obtained either spontaneously during chemostat cultivation (pBE-2) or selected during transconjugation (pBE-3 and pBE-4). The different insertion derivatives were tested for their stability...

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Detalles Bibliográficos
Autor principal: Bellani, Marina
Publicado: 1997
Materias:
threonine
article
auxotrophy
chemostat
escherichia coli
gene insertion sequence
operon
recombinant plasmid
temperature
Escherichia coli
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01415492_v19_n1_p1_Bellani
http://hdl.handle.net/20.500.12110/paper_01415492_v19_n1_p1_Bellani
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_01415492_v19_n1_p1_Bellani
record_format dspace
spelling paper:paper_01415492_v19_n1_p1_Bellani2023-06-08T15:11:16Z Tn 1000 (γδ) insertions stabilize pBR322-derived recombinant plasmids in Escherichia coli Bellani, Marina threonine article auxotrophy chemostat escherichia coli gene insertion sequence operon recombinant plasmid temperature Escherichia coli Tn1000 (or 'γδ') insertions in a pBR322-recombinant plasmid carrying the threonine operon (pBE) were obtained either spontaneously during chemostat cultivation (pBE-2) or selected during transconjugation (pBE-3 and pBE-4). The different insertion derivatives were tested for their stability in different host genetic backgrounds (in relation to threonine auxotrophy and recA function), various media composition (liquid or solid, rich or minimal) and growth temperatures (30°C, 37°C and 42°C). All the derivatives carrying the γδ sequence, albeit increasing their size from 9.7 to 15.6 Kb, significantly enhanced segregational and structural plasmid stability in every condition tested. Fil:Bellani, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1997 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01415492_v19_n1_p1_Bellani http://hdl.handle.net/20.500.12110/paper_01415492_v19_n1_p1_Bellani
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic threonine
article
auxotrophy
chemostat
escherichia coli
gene insertion sequence
operon
recombinant plasmid
temperature
Escherichia coli
spellingShingle threonine
article
auxotrophy
chemostat
escherichia coli
gene insertion sequence
operon
recombinant plasmid
temperature
Escherichia coli
Bellani, Marina
Tn 1000 (γδ) insertions stabilize pBR322-derived recombinant plasmids in Escherichia coli
topic_facet threonine
article
auxotrophy
chemostat
escherichia coli
gene insertion sequence
operon
recombinant plasmid
temperature
Escherichia coli
description Tn1000 (or 'γδ') insertions in a pBR322-recombinant plasmid carrying the threonine operon (pBE) were obtained either spontaneously during chemostat cultivation (pBE-2) or selected during transconjugation (pBE-3 and pBE-4). The different insertion derivatives were tested for their stability in different host genetic backgrounds (in relation to threonine auxotrophy and recA function), various media composition (liquid or solid, rich or minimal) and growth temperatures (30°C, 37°C and 42°C). All the derivatives carrying the γδ sequence, albeit increasing their size from 9.7 to 15.6 Kb, significantly enhanced segregational and structural plasmid stability in every condition tested.
author Bellani, Marina
author_facet Bellani, Marina
author_sort Bellani, Marina
title Tn 1000 (γδ) insertions stabilize pBR322-derived recombinant plasmids in Escherichia coli
title_short Tn 1000 (γδ) insertions stabilize pBR322-derived recombinant plasmids in Escherichia coli
title_full Tn 1000 (γδ) insertions stabilize pBR322-derived recombinant plasmids in Escherichia coli
title_fullStr Tn 1000 (γδ) insertions stabilize pBR322-derived recombinant plasmids in Escherichia coli
title_full_unstemmed Tn 1000 (γδ) insertions stabilize pBR322-derived recombinant plasmids in Escherichia coli
title_sort tn 1000 (γδ) insertions stabilize pbr322-derived recombinant plasmids in escherichia coli
publishDate 1997
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01415492_v19_n1_p1_Bellani
http://hdl.handle.net/20.500.12110/paper_01415492_v19_n1_p1_Bellani
work_keys_str_mv AT bellanimarina tn1000gdinsertionsstabilizepbr322derivedrecombinantplasmidsinescherichiacoli
_version_ 1768544951065378816

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