Oxygen radical formation in anoxic transgression and anoxia-reoxygenation: Foe or phantom? Experiments with a hypoxia tolerant bivalve
Intertidal blue mussels, Mytilus edulis, experience hypoxia reoxygenation during tidal emersion and resubmersion cycles, and this is often suggested to represent a major stress for the animals, especially for their respiratory tissues, the gills. We exposed mussels to experimental short and prolonge...
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2013
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01411136_v92_n_p110_RiveraIngraham http://hdl.handle.net/20.500.12110/paper_01411136_v92_n_p110_RiveraIngraham |
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paper:paper_01411136_v92_n_p110_RiveraIngraham2023-06-08T15:11:14Z Oxygen radical formation in anoxic transgression and anoxia-reoxygenation: Foe or phantom? Experiments with a hypoxia tolerant bivalve Rocchetta, Iara Blue mussel Live imaging Oxidative stress Reoxygenation Animals Cell death Histology Imaging techniques Metabolism Molluscs Oxidative stress Oxygen Proteins Tissue Antioxidant defense Blue mussels Experimental conditions Glutathione concentration Live imaging Reactive oxygen species Reoxygenation Respiratory response Tissue engineering caspase catalase glutathione hydrogen peroxide oxygen radical reactive oxygen metabolite succinic acid superoxide superoxide dismutase apoptosis bivalve hypoxia imaging method oxygen oxygenation respiration anaerobic growth animal experiment animal tissue anoxia article confocal microscopy controlled study gill hypoxia intertidal species lipid peroxidation metabolic rate Mytilus edulis nonhuman oxidation reduction state oxidative stress oxygen tension protein carbonylation reoxygenation Animalia Bivalvia Mytilus edulis Blue mussel Live imaging Oxidative stress Reoxygenation Animals Anoxia Antioxidants Caspases Gills Glutathione Hydrogen Peroxide Lipid Peroxidation Mytilus edulis Oxidative Stress Oxygen Reactive Oxygen Species Succinates Superoxides Intertidal blue mussels, Mytilus edulis, experience hypoxia reoxygenation during tidal emersion and resubmersion cycles, and this is often suggested to represent a major stress for the animals, especially for their respiratory tissues, the gills. We exposed mussels to experimental short and prolonged anoxia and subsequent reoxygenation and analyzed the respiratory response in excised gill tissue and the effects of treatment on reactive oxygen species (mainly ROS: superoxide anion, O2·- and hydrogen peroxide, H2O2), formation using live imaging techniques and confocal microscopy. Our aim was to understand if this "natural stress" would indeed produce oxidative damage and whether antioxidant defenses are induced under anoxia, to prevent oxidative damage during reoxygenation. Exposure to declining pO2 in the respiration chamber caused an increase of gill metabolic rate between 21 and 10kPa, a pO2 range in which whole animal respiration is reported to be oxyregulating. Exposure of the animals to severe anoxia caused an onset of anaerobiosis (succinate accumulation) and shifted high and low critical pc values (pc1: onset of oxyregulation in gills, pc2: switch from oxyregulation to oxyconformity) to higher pO2. Concentrations of both ROS decreased strongly during anoxic exposure of the mussels and increased upon reoxygenation. This ROS burst induced lipid peroxidation in the mantle, but neither were protein carbonyl levels increased (oxidative damage in the protein fraction), nor did the tissue glutathione concentration change in the gills. Further, analysis of apoptosis markers indicated no induction of cell death in the gills. To our knowledge, this is the first paper that directly measures ROS formation during anoxia reoxygenation in mussels. We conclude that hypoxia tolerant intertidal mussels do not suffer major oxidative stress in gill and mantle tissues under these experimental conditions. © 2013 Elsevier Ltd. Fil:Rocchetta, I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2013 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01411136_v92_n_p110_RiveraIngraham http://hdl.handle.net/20.500.12110/paper_01411136_v92_n_p110_RiveraIngraham |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Blue mussel Live imaging Oxidative stress Reoxygenation Animals Cell death Histology Imaging techniques Metabolism Molluscs Oxidative stress Oxygen Proteins Tissue Antioxidant defense Blue mussels Experimental conditions Glutathione concentration Live imaging Reactive oxygen species Reoxygenation Respiratory response Tissue engineering caspase catalase glutathione hydrogen peroxide oxygen radical reactive oxygen metabolite succinic acid superoxide superoxide dismutase apoptosis bivalve hypoxia imaging method oxygen oxygenation respiration anaerobic growth animal experiment animal tissue anoxia article confocal microscopy controlled study gill hypoxia intertidal species lipid peroxidation metabolic rate Mytilus edulis nonhuman oxidation reduction state oxidative stress oxygen tension protein carbonylation reoxygenation Animalia Bivalvia Mytilus edulis Blue mussel Live imaging Oxidative stress Reoxygenation Animals Anoxia Antioxidants Caspases Gills Glutathione Hydrogen Peroxide Lipid Peroxidation Mytilus edulis Oxidative Stress Oxygen Reactive Oxygen Species Succinates Superoxides |
| spellingShingle |
Blue mussel Live imaging Oxidative stress Reoxygenation Animals Cell death Histology Imaging techniques Metabolism Molluscs Oxidative stress Oxygen Proteins Tissue Antioxidant defense Blue mussels Experimental conditions Glutathione concentration Live imaging Reactive oxygen species Reoxygenation Respiratory response Tissue engineering caspase catalase glutathione hydrogen peroxide oxygen radical reactive oxygen metabolite succinic acid superoxide superoxide dismutase apoptosis bivalve hypoxia imaging method oxygen oxygenation respiration anaerobic growth animal experiment animal tissue anoxia article confocal microscopy controlled study gill hypoxia intertidal species lipid peroxidation metabolic rate Mytilus edulis nonhuman oxidation reduction state oxidative stress oxygen tension protein carbonylation reoxygenation Animalia Bivalvia Mytilus edulis Blue mussel Live imaging Oxidative stress Reoxygenation Animals Anoxia Antioxidants Caspases Gills Glutathione Hydrogen Peroxide Lipid Peroxidation Mytilus edulis Oxidative Stress Oxygen Reactive Oxygen Species Succinates Superoxides Rocchetta, Iara Oxygen radical formation in anoxic transgression and anoxia-reoxygenation: Foe or phantom? Experiments with a hypoxia tolerant bivalve |
| topic_facet |
Blue mussel Live imaging Oxidative stress Reoxygenation Animals Cell death Histology Imaging techniques Metabolism Molluscs Oxidative stress Oxygen Proteins Tissue Antioxidant defense Blue mussels Experimental conditions Glutathione concentration Live imaging Reactive oxygen species Reoxygenation Respiratory response Tissue engineering caspase catalase glutathione hydrogen peroxide oxygen radical reactive oxygen metabolite succinic acid superoxide superoxide dismutase apoptosis bivalve hypoxia imaging method oxygen oxygenation respiration anaerobic growth animal experiment animal tissue anoxia article confocal microscopy controlled study gill hypoxia intertidal species lipid peroxidation metabolic rate Mytilus edulis nonhuman oxidation reduction state oxidative stress oxygen tension protein carbonylation reoxygenation Animalia Bivalvia Mytilus edulis Blue mussel Live imaging Oxidative stress Reoxygenation Animals Anoxia Antioxidants Caspases Gills Glutathione Hydrogen Peroxide Lipid Peroxidation Mytilus edulis Oxidative Stress Oxygen Reactive Oxygen Species Succinates Superoxides |
| description |
Intertidal blue mussels, Mytilus edulis, experience hypoxia reoxygenation during tidal emersion and resubmersion cycles, and this is often suggested to represent a major stress for the animals, especially for their respiratory tissues, the gills. We exposed mussels to experimental short and prolonged anoxia and subsequent reoxygenation and analyzed the respiratory response in excised gill tissue and the effects of treatment on reactive oxygen species (mainly ROS: superoxide anion, O2·- and hydrogen peroxide, H2O2), formation using live imaging techniques and confocal microscopy. Our aim was to understand if this "natural stress" would indeed produce oxidative damage and whether antioxidant defenses are induced under anoxia, to prevent oxidative damage during reoxygenation. Exposure to declining pO2 in the respiration chamber caused an increase of gill metabolic rate between 21 and 10kPa, a pO2 range in which whole animal respiration is reported to be oxyregulating. Exposure of the animals to severe anoxia caused an onset of anaerobiosis (succinate accumulation) and shifted high and low critical pc values (pc1: onset of oxyregulation in gills, pc2: switch from oxyregulation to oxyconformity) to higher pO2. Concentrations of both ROS decreased strongly during anoxic exposure of the mussels and increased upon reoxygenation. This ROS burst induced lipid peroxidation in the mantle, but neither were protein carbonyl levels increased (oxidative damage in the protein fraction), nor did the tissue glutathione concentration change in the gills. Further, analysis of apoptosis markers indicated no induction of cell death in the gills. To our knowledge, this is the first paper that directly measures ROS formation during anoxia reoxygenation in mussels. We conclude that hypoxia tolerant intertidal mussels do not suffer major oxidative stress in gill and mantle tissues under these experimental conditions. © 2013 Elsevier Ltd. |
| author |
Rocchetta, Iara |
| author_facet |
Rocchetta, Iara |
| author_sort |
Rocchetta, Iara |
| title |
Oxygen radical formation in anoxic transgression and anoxia-reoxygenation: Foe or phantom? Experiments with a hypoxia tolerant bivalve |
| title_short |
Oxygen radical formation in anoxic transgression and anoxia-reoxygenation: Foe or phantom? Experiments with a hypoxia tolerant bivalve |
| title_full |
Oxygen radical formation in anoxic transgression and anoxia-reoxygenation: Foe or phantom? Experiments with a hypoxia tolerant bivalve |
| title_fullStr |
Oxygen radical formation in anoxic transgression and anoxia-reoxygenation: Foe or phantom? Experiments with a hypoxia tolerant bivalve |
| title_full_unstemmed |
Oxygen radical formation in anoxic transgression and anoxia-reoxygenation: Foe or phantom? Experiments with a hypoxia tolerant bivalve |
| title_sort |
oxygen radical formation in anoxic transgression and anoxia-reoxygenation: foe or phantom? experiments with a hypoxia tolerant bivalve |
| publishDate |
2013 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01411136_v92_n_p110_RiveraIngraham http://hdl.handle.net/20.500.12110/paper_01411136_v92_n_p110_RiveraIngraham |
| work_keys_str_mv |
AT rocchettaiara oxygenradicalformationinanoxictransgressionandanoxiareoxygenationfoeorphantomexperimentswithahypoxiatolerantbivalve |
| _version_ |
1768546716523429888 |