Ligninolytic enzymes of the white rot basidiomycete Trametes trogii
The white rot fungus Trametes trogii strain BAFC 463 produced laccase, manganese peroxidase, lignin peroxidase and cellobiose dehydrogenase, as well as two hydrogen peroxide-producing activities: glucose oxidizing activity and glyoxal oxidase. In high-N (40 mM N) cultures, the titres of laccase, MnP...
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2001
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paper:paper_01384988_v21_n2_p179_Levin2023-06-08T15:10:58Z Ligninolytic enzymes of the white rot basidiomycete Trametes trogii Levin, Laura Noemi Forchíassin, Flavia fungal enzyme glucose hydrogen peroxide laccase lignin lignin peroxidase manganese peroxidase polysorbate 80 tryptophan veratryl alcohol article Basidiomycetes biotechnology controlled study culture medium enzyme activity enzyme metabolism enzyme synthesis fungus culture fungus growth nonhuman pH supplementation Trametes The white rot fungus Trametes trogii strain BAFC 463 produced laccase, manganese peroxidase, lignin peroxidase and cellobiose dehydrogenase, as well as two hydrogen peroxide-producing activities: glucose oxidizing activity and glyoxal oxidase. In high-N (40 mM N) cultures, the titres of laccase, MnP and GLOX were 27 (6.55 U/ml), 45 (403.00 mU/ml) and 8 (32,14 mU/ml) fold higher, respectively, than those measured in an N-limited medium. This is consistent with the fact that the ligninolytic system of T. trogii is expressed constitutively. Lower activities of all the enzymes tested were recorded upon decreasing the initial pH of the medium from 6.5 to 4.5. Adding veratryl alcohol improved GLOX production, while laccase activity was stimulated by tryptophan. Supplying Tween 80 strongly reduced the activity of both MnP and GLOX, but increased laccase production. The titre of MnP was affected by the concentration of Mn in the culture medium, the highest levels were obtained with 90 μM Mn (II). LiP activity, as CDH activity, were detected only in the medium supplemented with sawdust. In this medium, laccase production reached a maximum of 4.75 U/ml, MnP 747.60 mU/ml and GLOX 117.11 mU/ml. LiP, MnP and GLOX activities were co-induced, attaining their highest levels at the beginning of secondary metabolism, but while MnP, laccase, GLOX and CDH activities were also present in the primary growth phase, LiP activity appears to be idiophasic. The simultaneous presence of high ligninolytic and hydrogen peroxide producing activities in this fungus makes it an attractive microorganism for future biotechnological applications. Fil:Levin, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Forchiassin, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2001 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01384988_v21_n2_p179_Levin http://hdl.handle.net/20.500.12110/paper_01384988_v21_n2_p179_Levin |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
fungal enzyme glucose hydrogen peroxide laccase lignin lignin peroxidase manganese peroxidase polysorbate 80 tryptophan veratryl alcohol article Basidiomycetes biotechnology controlled study culture medium enzyme activity enzyme metabolism enzyme synthesis fungus culture fungus growth nonhuman pH supplementation Trametes |
| spellingShingle |
fungal enzyme glucose hydrogen peroxide laccase lignin lignin peroxidase manganese peroxidase polysorbate 80 tryptophan veratryl alcohol article Basidiomycetes biotechnology controlled study culture medium enzyme activity enzyme metabolism enzyme synthesis fungus culture fungus growth nonhuman pH supplementation Trametes Levin, Laura Noemi Forchíassin, Flavia Ligninolytic enzymes of the white rot basidiomycete Trametes trogii |
| topic_facet |
fungal enzyme glucose hydrogen peroxide laccase lignin lignin peroxidase manganese peroxidase polysorbate 80 tryptophan veratryl alcohol article Basidiomycetes biotechnology controlled study culture medium enzyme activity enzyme metabolism enzyme synthesis fungus culture fungus growth nonhuman pH supplementation Trametes |
| description |
The white rot fungus Trametes trogii strain BAFC 463 produced laccase, manganese peroxidase, lignin peroxidase and cellobiose dehydrogenase, as well as two hydrogen peroxide-producing activities: glucose oxidizing activity and glyoxal oxidase. In high-N (40 mM N) cultures, the titres of laccase, MnP and GLOX were 27 (6.55 U/ml), 45 (403.00 mU/ml) and 8 (32,14 mU/ml) fold higher, respectively, than those measured in an N-limited medium. This is consistent with the fact that the ligninolytic system of T. trogii is expressed constitutively. Lower activities of all the enzymes tested were recorded upon decreasing the initial pH of the medium from 6.5 to 4.5. Adding veratryl alcohol improved GLOX production, while laccase activity was stimulated by tryptophan. Supplying Tween 80 strongly reduced the activity of both MnP and GLOX, but increased laccase production. The titre of MnP was affected by the concentration of Mn in the culture medium, the highest levels were obtained with 90 μM Mn (II). LiP activity, as CDH activity, were detected only in the medium supplemented with sawdust. In this medium, laccase production reached a maximum of 4.75 U/ml, MnP 747.60 mU/ml and GLOX 117.11 mU/ml. LiP, MnP and GLOX activities were co-induced, attaining their highest levels at the beginning of secondary metabolism, but while MnP, laccase, GLOX and CDH activities were also present in the primary growth phase, LiP activity appears to be idiophasic. The simultaneous presence of high ligninolytic and hydrogen peroxide producing activities in this fungus makes it an attractive microorganism for future biotechnological applications. |
| author |
Levin, Laura Noemi Forchíassin, Flavia |
| author_facet |
Levin, Laura Noemi Forchíassin, Flavia |
| author_sort |
Levin, Laura Noemi |
| title |
Ligninolytic enzymes of the white rot basidiomycete Trametes trogii |
| title_short |
Ligninolytic enzymes of the white rot basidiomycete Trametes trogii |
| title_full |
Ligninolytic enzymes of the white rot basidiomycete Trametes trogii |
| title_fullStr |
Ligninolytic enzymes of the white rot basidiomycete Trametes trogii |
| title_full_unstemmed |
Ligninolytic enzymes of the white rot basidiomycete Trametes trogii |
| title_sort |
ligninolytic enzymes of the white rot basidiomycete trametes trogii |
| publishDate |
2001 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01384988_v21_n2_p179_Levin http://hdl.handle.net/20.500.12110/paper_01384988_v21_n2_p179_Levin |
| work_keys_str_mv |
AT levinlauranoemi ligninolyticenzymesofthewhiterotbasidiomycetetrametestrogii AT forchiassinflavia ligninolyticenzymesofthewhiterotbasidiomycetetrametestrogii |
| _version_ |
1768543987090587648 |