Culture conditions for the production of pectinolytic enzymes by the white-rot fungus Trametes trogii on a laboratory scale
Different cultural parameters that regulate pectinolytic enzyme production in vitro by Trametes trogii were studied. When grown in a medium containing pectin, T. trogii produced extracellular polymethylgalacturonase, polygalacturonase and pectin lyase but no pectate lyase activity. No significant di...
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1998
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01384988_v18_n2_p157_Levin http://hdl.handle.net/20.500.12110/paper_01384988_v18_n2_p157_Levin |
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paper:paper_01384988_v18_n2_p157_Levin2023-06-08T15:10:58Z Culture conditions for the production of pectinolytic enzymes by the white-rot fungus Trametes trogii on a laboratory scale Levin, Laura Noemi Forchíassin, Flavia carboxymethylcellulose casamino acid glucose macrogol nitrogen pectate lyase pectin polygalacturonase polysorbate 20 polysorbate 80 triton x 100 xylan article enzyme activity enzyme induction enzyme synthesis fungus culture fungus growth laboratory ph phanerochaete Different cultural parameters that regulate pectinolytic enzyme production in vitro by Trametes trogii were studied. When grown in a medium containing pectin, T. trogii produced extracellular polymethylgalacturonase, polygalacturonase and pectin lyase but no pectate lyase activity. No significant differences in the maximum enzyme activities measured were observed with the addition of xylan, carboxymethylcellulose or both to the medium containing pectin. The addition of glucose to that medium considerably decreased all the activities studied, and in a medium with glucose as the sole carbon source no galacturonase activity could be measured, and pectin lyase activity was at its minimum. The low synthesis of pectin lyase in cultures containing glucose suggests that this enzyme is constitutive in contrast to the polygalacturonases that were not detected. The increase in pectin concentration stimulated growth and enzyme production. The highest specific activities were attained with the greatest concentration tested (15 g/l). Casamino acids were the best nitrogen source for enzyme production. Maximum growth was measured at pH 3.3; pH values of around 4.5 stimulated enzyme production, but high pectinase activities were also detected in media with more alkaline initial pH values (6.2 for galacturonases and 6.6 for lyases), probably owing to the specific induction of particular isoforms. In the range of 23 to 28°C, good results were obtained in growth as well as in enzyme production. The addition of Tween 80 promoted growth and Save the highest yield of polymethylgalacturonase and pectin lyase (0.37 and 36.2 E.U./ml, respectively). The highest polygalacturonase activity (1.1 E.U./ml) was achieved with polyethylene glycol. Tween 20 and Triton X-100 inhibited growth and pectinase production. Fil:Levin, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Forchiassin, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1998 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01384988_v18_n2_p157_Levin http://hdl.handle.net/20.500.12110/paper_01384988_v18_n2_p157_Levin |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
carboxymethylcellulose casamino acid glucose macrogol nitrogen pectate lyase pectin polygalacturonase polysorbate 20 polysorbate 80 triton x 100 xylan article enzyme activity enzyme induction enzyme synthesis fungus culture fungus growth laboratory ph phanerochaete |
| spellingShingle |
carboxymethylcellulose casamino acid glucose macrogol nitrogen pectate lyase pectin polygalacturonase polysorbate 20 polysorbate 80 triton x 100 xylan article enzyme activity enzyme induction enzyme synthesis fungus culture fungus growth laboratory ph phanerochaete Levin, Laura Noemi Forchíassin, Flavia Culture conditions for the production of pectinolytic enzymes by the white-rot fungus Trametes trogii on a laboratory scale |
| topic_facet |
carboxymethylcellulose casamino acid glucose macrogol nitrogen pectate lyase pectin polygalacturonase polysorbate 20 polysorbate 80 triton x 100 xylan article enzyme activity enzyme induction enzyme synthesis fungus culture fungus growth laboratory ph phanerochaete |
| description |
Different cultural parameters that regulate pectinolytic enzyme production in vitro by Trametes trogii were studied. When grown in a medium containing pectin, T. trogii produced extracellular polymethylgalacturonase, polygalacturonase and pectin lyase but no pectate lyase activity. No significant differences in the maximum enzyme activities measured were observed with the addition of xylan, carboxymethylcellulose or both to the medium containing pectin. The addition of glucose to that medium considerably decreased all the activities studied, and in a medium with glucose as the sole carbon source no galacturonase activity could be measured, and pectin lyase activity was at its minimum. The low synthesis of pectin lyase in cultures containing glucose suggests that this enzyme is constitutive in contrast to the polygalacturonases that were not detected. The increase in pectin concentration stimulated growth and enzyme production. The highest specific activities were attained with the greatest concentration tested (15 g/l). Casamino acids were the best nitrogen source for enzyme production. Maximum growth was measured at pH 3.3; pH values of around 4.5 stimulated enzyme production, but high pectinase activities were also detected in media with more alkaline initial pH values (6.2 for galacturonases and 6.6 for lyases), probably owing to the specific induction of particular isoforms. In the range of 23 to 28°C, good results were obtained in growth as well as in enzyme production. The addition of Tween 80 promoted growth and Save the highest yield of polymethylgalacturonase and pectin lyase (0.37 and 36.2 E.U./ml, respectively). The highest polygalacturonase activity (1.1 E.U./ml) was achieved with polyethylene glycol. Tween 20 and Triton X-100 inhibited growth and pectinase production. |
| author |
Levin, Laura Noemi Forchíassin, Flavia |
| author_facet |
Levin, Laura Noemi Forchíassin, Flavia |
| author_sort |
Levin, Laura Noemi |
| title |
Culture conditions for the production of pectinolytic enzymes by the white-rot fungus Trametes trogii on a laboratory scale |
| title_short |
Culture conditions for the production of pectinolytic enzymes by the white-rot fungus Trametes trogii on a laboratory scale |
| title_full |
Culture conditions for the production of pectinolytic enzymes by the white-rot fungus Trametes trogii on a laboratory scale |
| title_fullStr |
Culture conditions for the production of pectinolytic enzymes by the white-rot fungus Trametes trogii on a laboratory scale |
| title_full_unstemmed |
Culture conditions for the production of pectinolytic enzymes by the white-rot fungus Trametes trogii on a laboratory scale |
| title_sort |
culture conditions for the production of pectinolytic enzymes by the white-rot fungus trametes trogii on a laboratory scale |
| publishDate |
1998 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01384988_v18_n2_p157_Levin http://hdl.handle.net/20.500.12110/paper_01384988_v18_n2_p157_Levin |
| work_keys_str_mv |
AT levinlauranoemi cultureconditionsfortheproductionofpectinolyticenzymesbythewhiterotfungustrametestrogiionalaboratoryscale AT forchiassinflavia cultureconditionsfortheproductionofpectinolyticenzymesbythewhiterotfungustrametestrogiionalaboratoryscale |
| _version_ |
1768543174206160896 |